Abstract:
:Glucosamine (GlcN) has been reported to possess several biomedical properties, and currently a great deal of attention has been focused on improving the functional properties of GlcN for different applications. Therefore, this study was conducted to introduce a carboxybutyryl functional group to GlcN and to find out the inhibitory mechanism of a novel GlcN derivative, carboxybutyrylated GlcN (CGlcN), on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in bacterial lipopolysaccharide (LPS)-induced mouse macrophages (RAW264.7 cells). In the initial experiments, the production of NO and prostaglandin E(2) (PGE(2)) was inhibited by CGlcN pretreatment and suggested the possibility of down-regulating their respective genes, iNOS and COX-2. Reverse transcription-polymerase chain reaction and Western blot analysis revealed that CGlcN can affect both transcriptional and translational levels of iNOS and COX-2 expression. The data from the nuclear factor-kappaB (NF-kappaB) promoter gene transfection experiment supported the idea that inhibition of iNOS and COX-2 is caused by the down-regulation of their transcription factor, NF-kappaB. Following stimulation with LPS, p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinase (JNK) present upstream of NF-kappaB signaling were also inhibited by CGlcN treatment. However, the protein level of another MAPK, extracellular signal-regulated kinase (ERK), remained unaffected. Moreover, following treatment with CGlcN, the protein expression of I-kappaB kinase (IKK) clearly confirmed that its down-regulation directly inhibited the degradation of IkappaB and release of NF-kappaB. Therefore, it can be concluded that CGlcN is capable of inhibiting iNOS and COX-2 expression in LPS-induced RAW264.7 cells via attenuation of NF-kappaB signaling by p38 MAPK and JNK, but not by ERK.
journal_name
Immunologyjournal_title
Immunologyauthors
Rajapakse N,Kim MM,Mendis E,Kim SKdoi
10.1111/j.1365-2567.2007.02683.xsubject
Has Abstractpub_date
2008-03-01 00:00:00pages
348-57issue
3eissn
0019-2805issn
1365-2567pii
IMM2683journal_volume
123pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::MUC1 is a transmembrane mucin that is expressed on ductal epithelial cells and epithelial malignancies and has been proposed as a target antigen for immunotherapy. The expression of MUC1 has recently been reported on T and B cells. In this study we demonstrate that following activation in vivo or activation by differe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01562.x
更新日期:2003-01-01 00:00:00
abstract::Receptors for the Fc fragment of IgG (Fc gamma R) have a well-documented role in the generation of oxidative burst. It is tempting to speculate that the type of interaction with Fc gamma R could be a mechanism of regulation of this process. Here we report on a comparative study of the induction of oxidative burst in h...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::We have studied the antibody response in vitro of spleen cells from C57BL/6 mice kept on a protein deficient (D) or a normal diet (N). Short or long term protein restriction initiated after weaning led to increased plaque forming cell (PFC) responses to sheep red blood cells (SRBC), TNP-ficoll and TNP lipopolysacchari...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::A superantigen (Streptococcus pyogenes mitogen-2; SPM-2) that stimulates human helper T cells bearing unique types of variable domains of T-cell receptor beta-chain (TCR V beta) was isolated from the culture supernatant of S. pyogenes strain T12. The active molecule isolated by diethylaminoethyl (DEAE)-cellulose chrom...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00277.x
更新日期:1997-07-01 00:00:00
abstract::Nod-like receptors are a family of innate immune receptors that link cytosolic sensing of microbial and danger stimuli to the activation of immune responses. Two Nod-like receptor family members, Nod1 and Nod2, recognize bacterial peptidoglycan and activate immune responses via nuclear factor-κB (NF-κB) and mitogen-ac...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12307
更新日期:2014-10-01 00:00:00
abstract::A panel of four mimotopes of the epitope recognized by the highly protective monoclonal antibody against Schistosoma mansoni (152-66-9B) was obtained by screening a solid-phase 8mer random peptide library. Three of the four mimotopes (p28, p29 and p30) were efficiently recognized in an in vitro radioimmunoassay by the...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00139.x
更新日期:2000-12-01 00:00:00
abstract::Passive transfer of immunity to Taenia taeniaeformis was achieved with serum taken 14, 21, 49 and 63 days after infection. The protective capacity of serum collected at 14 and 21 days resided in the 7Sgamma2 immunoglobulins and appeared to be partics the infection progressed the range of chromatographic fractions show...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-11-01 00:00:00
abstract::It was found that spleen cells from mice injected i.v. with large doses of BCG respond to PHA stimulation less intensely than do normal spleen cells. We were able to show that nylon wool column purified BCG treated T cells also had a low PHA reactivity. Unfractionated spleen cells, adherent cells or T-enriched populat...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::The defined antigen substrate spheres (DASS) system was employed for analysing reactions between solid state antibody or antigen and soluble immune complexes. Sepharose beads covalently coupled with ovalbumin were used to represent tumour cells and beads coupled with antibody against ovalbumin were used to represent a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
abstract::Thrombomodulin is a transmembranous glycoprotein of endothelial cells. In vitro it is a marker of endothelial cell injury. In vivo the levels of serum thrombomodulin are regarded as a parameter of activity in vasculitides. The latter are pathophysiologically determined by neutrophil-derived inflammation and endothelia...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01469.x
更新日期:2002-11-01 00:00:00
abstract::Cholera toxin (CT) or its subunits were given orally to mice and division of intestinal intraepithelial lymphocytes (IEL) in vivo measured by double immunofluorescence using 5-bromo-2'-deoxyuridine (BRdU) and membrane alpha beta T-cell receptors (TCR) or gamma delta TCR staining in frozen sections. Cholera toxin (10 m...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-721.x
更新日期:1996-09-01 00:00:00
abstract::Human polymorphonuclear granulocytes (PMN) generate the inflammatory mediator leukotriene B4 (LTB4) as a response to cell activation. In addition, PMN inactivate LTB4 by omega-oxidation resulting in the formation of 20-OH- and 20-COOH-LTB4. The transport of exogenous LTB4 to the metabolizing enzymes is mediated via hi...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::Staphylococcus aureus is a major pathogen that can cause a broad spectrum of serious infections including skin infections, pneumonia and sepsis. Peritoneal mast cells have been implicated in the host response towards various bacterial insults and to provide mechanistic insight into the role of mast cells in intraperit...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12297
更新日期:2014-10-01 00:00:00
abstract::Immunoglobulin E (IgE)-dependent mechanisms play a pivotal role in mediating allergic disease. Previously, VH-Cepsilon transcripts from blood or spleen of atopic asthmatics have been analysed for VH gene usage and patterns of somatic mutation. An over-representation of the minor VH5 family has been observed, consisten...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00910.x
更新日期:1999-12-01 00:00:00
abstract::It has previously been shown in mice that activated peritoneal macrophages, when first removed from the peritoneal cavity, suppress mitogen-stimulated lymphocyte transformation in vitro as measured by [3H]-thymidine incorporation. In this study, it is demonstrated that activated macrophages that have been incubated al...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::Urokinase-type plasminogen activator (u-PA), tumour necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) activities were measured for highly purified human monocytes cultured for 18 hr with recombinant human interleukin-3 (IL-3). IL-3 alone stimulated monocyte u-PA activity, but not TNF-alpha or IL-1 activity. H...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::Although it is well known that cases with silicosis exhibit various immunological abnormalities, the mechanisms involved in the occurrence of immuno-dysfunction or dysregulation induced by silica compounds have not yet been determined. Fas is a well-known cell surface molecule that is involved in the apoptosis pathway...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00509.x
更新日期:1998-06-01 00:00:00
abstract::Innate immune responses vary in a circadian manner, and more recent investigations aim to understand the underlying molecular mechanisms. Cytokine production varies significantly over the course of a day depending on the time of stimulation by pathogens or Toll-like receptor ligands, and multiple signaling pathways li...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13053
更新日期:2019-06-01 00:00:00
abstract::BLT mice, constructed by surgical implantation of human fetal thymus-liver tissues and intravenous delivery of autologous CD34+ haematopoietic stem cells into adult non-obese diabetic/severe combined immunodeficiency mice, were evaluated for vaccine-induced humoral immune responses. Following engraftment, these mice d...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03501.x
更新日期:2011-12-01 00:00:00
abstract::Supernatants of IL-2-activated mononuclear cells (MNC) that displayed an optimal lymphokine-activated killer (LAK) cell activity at 48-72 hr in culture were found to contain increased levels of tumour necrosis factor alpha (TNF alpha), interleukin-1 alpha (IL-1 alpha) and interferon-gamma (IFN-gamma) when compared wit...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::Prolonged exposure to ultraviolet light (UV) is known to lead to premature skin ageing, increased incidence of cataract and a high risk of developing skin cancers. UV-B irradiation, even if given as a single suberythemal dose, suppresses some immune responses, possibly reducing the production of T helper (Th) 1 cytoki...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.d01-2292.x
更新日期:1997-09-01 00:00:00
abstract::Initial separation and concentration of Clq from fresh, normal equine serum was accomplished by precipitation in 0.02 M acetate buffer, pH 5.5, at 4 degrees for 24 h. The re-dissolved precipitate was clarified by centrifugation at 80,000 g for 1 h and then dialysed against Tris-HCl buffer (0.05 M, pH 8.0) containing 1...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-07-01 00:00:00
abstract::It is increasingly becoming clear that various immune cells are infected by the very pathogens that they are supposed to attack. Although many mechanisms for microbial entry exist, it appears that a common route of entry shared by certain bacteria, viruses and parasites involves cellular lipid-rich microdomains someti...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2567.2001.01173.x
更新日期:2001-01-01 00:00:00
abstract::Activation of self-reactive T cells in healthy adults is prevented by the presence of autoantigen-specific CD4+CD25+ regulatory T cells (CD25+ Treg). To explore the functional development of autoantigen-reactive CD25+ Treg in humans we investigated if thymic CD25+ Treg from children aged 2 months to 11 years and cord ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02186.x
更新日期:2005-08-01 00:00:00
abstract::We have used a number of in vitro and in vivo techniques to identify the molecules that can bind to the cytoplasmic tail of the Ly49A receptor. Affinity chromatography using peptides corresponding to the N-terminal 18 amino acids of Ly49A allowed the recovery of a number of proteins that bound preferentially to the ty...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00825.x
更新日期:1999-08-01 00:00:00
abstract::Major histocompatibility complex (MHC) class I-specific inhibitory natural killer receptors (iNKRs) are expressed by subsets of T cells but the mechanisms inducing their expression are poorly understood, particularly for killer-cell immunoglobulin-like receptors (KIRs). The iNKRs are virtually absent from the surface ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03194.x
更新日期:2010-03-01 00:00:00
abstract::Vitamins A and E and select flavonoids in the family of catechins are well-defined small molecules that, if proven to possess immunomodulatory properties, hold promise as vaccine adjuvants and various therapies. In an effort to determine the in vivo immunomodulatory properties of these molecules, we found that althoug...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12614
更新日期:2016-08-01 00:00:00
abstract::Functionally defined mouse Lyt-1 and Lyt-2 cell sets were selected from peripheral lymph nodes by cytotoxic elimination with anti-Lyt monoclonal antibodies. The selected populations were labelled with 51Cr or [3H]-adenosine and traced in syngeneic recipients at 1, 24, 45 and 65 hr after intravenous injection. Recovere...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::We produced three murine monoclonal antibodies (mAb) directed against the human T-cell differentiation antigen CD2 (formerly T11) and analysed their epitope specificity by E-rosette inhibition, cross-blocking and proliferation-induction experiments. When added together, two mAb (both IgG1 kappa) were able to induce pr...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract::CD248 (endosialin) is a transmembrane glycoprotein that is dynamically expressed on pericytes and fibroblasts during tissue development, tumour neovascularization and inflammation. Its role in tissue remodelling is associated with increased stromal cell proliferation and migration. We show that CD248 is also uniquely ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03437.x
更新日期:2011-07-01 00:00:00