当前位置： SCI文献检索 > IMMUNOLOGY期刊下所有文献 > Kinetics of thrombomodulin release and endothelial cell injury by neutrophil-derived proteases and oxygen radicals.

Kinetics of thrombomodulin release and endothelial cell injury by neutrophil-derived proteases and oxygen radicals.

Abstract：

:Thrombomodulin is a transmembranous glycoprotein of endothelial cells. In vitro it is a marker of endothelial cell injury. In vivo the levels of serum thrombomodulin are regarded as a parameter of activity in vasculitides. The latter are pathophysiologically determined by neutrophil-derived inflammation and endothelial cell injury caused by secretion of proteases and hydrogen peroxide. It was the objective of this study to determine whether thrombomodulin is only a late marker of advanced endothelial cell injury or whether it indicates also earlier stages of cell alterations. Over 24 hr endothelial cell cultures were incubated with hydrogen peroxide or the neutrophil proteases proteinase-3, elastase and cathepsin G. The time-dependent increase of thrombomodulin in the supernatant was determined by enzyme-linked immunosorbent assay and immunoblot. In addition the viability (eosin, tetrazolium dye assay), detachment (crystal-violet assay), and apoptosis (4',6-diamine-2'-phenylindole-dihydrochloride assay) of the respective endothelial cells were determined for adherent and non-adherent cells. A rapid thrombomodulin increase was found under all experimental conditions. The additional immunoblotting analysis showed the pattern of proteolytic cleavage caused by the protease reactivity. In case of hydrogen peroxide the thrombomodulin increase was closely correlated with the loss of cell viability and lysis. The incubation of endothelial cells with the different proteases resulted in a time-dependent detachment of primarily viable cells. In addition to cell necrosis apoptotic cell death was found in the subgroup of detached endothelial cells after prolonged incubation over 24 hr with proteinase-3 (23%), elastase (31%), and cathepsin G (19%). In contrast, still adhering cells did not show any signs of necrosis or apoptosis. In summary these studies confirm in vitro that soluble thrombomodulin is not only a parameter of advanced endothelial cell destruction itself but also in addition an early marker of initial endothelial cell membrane changes induced by neutrophil derived proteases and oxygen radicals.

journal_name

Immunology

journal_title

Immunology

authors

Boehme MW,Galle P,Stremmel W

doi

10.1046/j.1365-2567.2002.01469.x

keywords:

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

340-9

issue

3

eissn

0019-2805

issn

1365-2567

pii

1469

journal_volume

107

pub_type

杂志文章

相关文献

IMMUNOLOGY文献大全
  • Production of interleukin-1-like cytokine by cultured rat glomerular macrophages.

    abstract：:Resident glomerular macrophages from normal rats were isolated and grown through long-term cultures in order to obtain confluent cell monolayers. These cells have a secretory function as revealed by the production of a cytokine with a molecular weight similar to interleukin-1 (IL-1). The demonstration that rat glomeru...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Mampaso F,Bricio T,Martin A,Molina A

    更新日期：1992-07-01 00:00:00

  • Molecular cloning and functional characterization of the pig homologue of integrin-associated protein (IAP/CD47).

    abstract：:We report the cloning of cDNA encoding the pig homologue of human integrin-associated protein (IAP or CD47). A pig CD47-specific probe was generated by polymerase chain reaction (PCR) amplification of pig leucocyte cDNA, using primers based on consensus regions among the known sequences of CD47 from different species....

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.2002.01465.x

    authors： Shahein YE,de Andrés DF,Pérez de la Lastra JM

    更新日期：2002-08-01 00:00:00

  • Mechanisms of resistance against experimental Trypanosoma cruzi infection. Requirements for cellular destruction of circulating forms of T. cruzi in human and murine in vitro systems.

    abstract：:The sensitivity of host (circulating) forms of T. cruzi to cell-mediated immunological destruction and requirements for the reaction were examined. Both human and mouse leucocytes were found to kill significant numbers of parasites in the presence of specific antibodies against the flagellates. Antibody involvement wa...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Kierszenbaum F,Hayes MM

    更新日期：1980-05-01 00:00:00

  • CD44 isoforms containing exons V6 and V7 are differentially expressed on mitogenically stimulated normal and Epstein-Barr virus-transformed human B cells.

    abstract：:CD44, a cell adhesion molecule, exists in multiple isoforms that are generated by RNA alternative splicing. CD44 isoforms containing exon V6 (CD44 V6) have been associated with tumorigenesis and metastasis. We investigated the association between human B-cell activation and CD44 V6 isoform expression by analysing its ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Kryworuckho M,Diaz-Mitoma F,Kumar A

    更新日期：1995-09-01 00:00:00

  • Various members of the Toll-like receptor family contribute to the innate immune response of human epidermal keratinocytes.

    abstract：:Toll-like receptors (TLRs) are important pattern recognition molecules that activate the nuclear factor (NF)-kappaB pathway leading to the production of antimicrobial immune mediators. As keratinocytes represent the first barrier against exogenous pathogens in human skin, we investigated their complete functional TLR1...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2005.02122.x

    authors： Köllisch G,Kalali BN,Voelcker V,Wallich R,Behrendt H,Ring J,Bauer S,Jakob T,Mempel M,Ollert M

    更新日期：2005-04-01 00:00:00

  • Evidence for a novel HLA antigen found on human extravillous trophoblast and a choriocarcinoma cell line.

    abstract：:We describe the characterization of a novel HLA Class I molecule, which we have isolated from chorionic cytotrophoblast cell membranes, and from a trophoblast-derived choriocarcinoma cell line, BeWo; classical HLA Class I antigens are not expressed on these cells. This antigen is an electrophoretically non-polymorphic...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Ellis SA,Sargent IL,Redman CW,McMichael AJ

    更新日期：1986-12-01 00:00:00

  • The influence of cigarette tobacco smoke products on the immune response. The cellular basis of immunosuppression by a water-soluble condensate of tobacco smoke.

    abstract：:The immunosuppression exhibited by a water-soluble condensate of tobacco smoke (WSC) has been studied in vivo and in vitro. When multiple sublethal doses of WSC were injected into C57Bl/6 mice, their ability to respond to immunization with sheep erythrocytes by the formation of plaque-forming cells was severely inhibi...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Jacob CV,Stelzer GT,Wallace JH

    更新日期：1980-08-01 00:00:00

  • Mycobacterial antigen-specific human T-cell clones secreting macrophage activating factors.

    abstract：:Macrophage activating factor (MAF) is produced by antigen-stimulated lymphocytes and activates macrophages for antimicrobial function. The capacity of individual microbial antigens to evoke and regulate this response has been explored using an affinity purified antigen (TB68) of Mycobacterium tuberculosis in combinati...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Matthews R,Scoging A,Rees AD

    更新日期：1985-01-01 00:00:00

  • mTOR: taking cues from the immune microenvironment.

    abstract：:The ultimate outcome of T cell receptor recognition is determined by the context in which the antigen is encountered. In this fashion both antigen-presenting cells and T cells must integrate multiple environmental cues in the form of pathogen-associated molecular patterns, cytokines and accessory molecule signals. The...

    journal_title：Immunology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2567.2009.03125.x

    authors： Delgoffe GM,Powell JD

    更新日期：2009-08-01 00:00:00

  • Toll-like receptor 4 signalling through MyD88 is essential to control Salmonella enterica serovar typhimurium infection, but not for the initiation of bacterial clearance.

    abstract：:Toll-like receptor-4 (TLR4) is important in protection against lethal Salmonella enterica serovar Typhimurium (S. Typhimurium) infection. Control of the early stages of sublethal S. Typhimurium infection in mice depends on TLR4-dependent activation of macrophages and natural killer (NK) cells to drive an inflammatory ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2009.03146.x

    authors： Talbot S,Tötemeyer S,Yamamoto M,Akira S,Hughes K,Gray D,Barr T,Mastroeni P,Maskell DJ,Bryant CE

    更新日期：2009-12-01 00:00:00

  • The genetic contribution to human T-cell receptor repertoire.

    abstract：:Recent studies in mice have highlighted the importance of polymorphic genetic loci such as the major histocompatibility complex (MHC) or minor lymphocyte-stimulating antigen (Mls) in determining the nature of the peripheral T-cell receptor (TcR) population. As our knowledge of the equivalent process in humans is incom...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Loveridge JA,Rosenberg WM,Kirkwood TB,Bell JI

    更新日期：1991-10-01 00:00:00

  • Following TRAIL's path in the immune system.

    abstract：:The members of the tumour necrosis factor (TNF) superfamily of cytokines play important roles in the regulation of various immune-cell functions. Likewise, induction of cell death by apoptosis is indispensable for the normal functioning of the immune system. There are two major pathways of apoptosis induction. The int...

    journal_title：Immunology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2567.2009.03058.x

    authors： Falschlehner C,Schaefer U,Walczak H

    更新日期：2009-06-01 00:00:00

  • Proline-rich polypeptide from ovine colostrum: its effect on skin permeability and on the immune response.

    abstract：:The effect of a proline-rich polypeptide from ovine colostrum on the humoral immune response in mice against sheep red blood cells (SRBC) was studied. It was shown that the polypeptide had a regulatory activity stimulating or suppressing the immune response. The proline-rich polypeptide also increased the permeability...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Wieczorek Z,Zimecki M,Janusz M,Staroscik K,Lisowski J

    更新日期：1979-04-01 00:00:00

  • Binding of human IgA1 to rat peritoneal macrophages.

    abstract：:In the present study we have investigated whether bovine erythrocytes (Eb) specifically sensitized with human polyclonal IgA1 (Eb-IgA1) are able to bind to resident adherent rat peritoneal cells (PM phi). Rat PM phi formed rosettes with Eb-IgA1 at room temperature and at 37 degrees. The formation of these rosettes cou...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Gorter A,Hiemstra PS,van der Voort EA,van Es LA,Daha MR

    更新日期：1988-06-01 00:00:00

  • IL-13 has only a subset of IL-4-like activities on B chronic lymphocytic leukaemia cells.

    abstract：:The recently described interleukin-13 (IL-13) has been shown to share many of the effects of IL-4 on normal B cells, including growth-promoting activity and induction of CD23. In this study, we compared the effects of IL-13 and IL-4 on B chronic lymphocytic leukaemias (B-CLL) cells. After anti-CD40 activation, both IL...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Fluckiger AC,Brière F,Zurawski G,Bridon JM,Banchereau J

    更新日期：1994-11-01 00:00:00

  • Inhibition of interleukin-2 by a Gram-positive bacterium, Streptococcus mutans.

    abstract：:Generation of an effective cellular immune response is key to the successful development of both humoral and cellular immune defences against most pathogens. However, while the type of cellular immune response elicited by any given pathogen is dictated by the entire array of antigens and molecules which comprise that ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.1998.00631.x

    authors： Plitnick LM,Banas JA,Jelley-Gibbs DM,O'neil J,Christian T,Mudzinski SP,Gosselin EJ

    更新日期：1998-12-01 00:00:00

  • Interleukin-7 inhibits pre-T-cell differentiation induced by the pre-T-cell receptor signal and the effect is mimicked by hGM-CSF in hGM-CSF receptor transgenic mice.

    abstract：:We have previously reported that human granulocyte-macrophage colony-stimulating factor (hGM-CSF) causes a stage-specific inhibition of T-cell receptor (TCR) alphabeta cell development in the thymus of transgenic mice constitutively expressing the hGM-CSF receptor. Since it has been reported that the addition of inter...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.2002.01402.x

    authors： Yasuda Y,Kaneko A,Nishijima I,Miyatake S,Arai K

    更新日期：2002-06-01 00:00:00

  • The T-cell response to haptenated insulins. I. The proliferative response.

    abstract：:Mice were primed with TNP-derivatized insulin, or TNP-Mycobacteria, and lymph node cells were challenged in vitro with haptenated and unhaptenated antigens. Using either priming antigen, T-cell proliferative responses could be obtained to TNP-insulin. In B10 (H-2b), mice, which are responders to beef insulin (BI), but...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Wallace GR,Briffa J,McCafferty I,Askenase PW,Chain BM

    更新日期：1989-01-01 00:00:00

  • The effect of various immunosuppressive agents on mouse peritoneal macrophages and on the in vitro phagocytosis of Escherichia coli O4:K3:H5 and degradation of 125I-labelled HSA-antibody complexes by these cells.

    abstract：:Large doses of hydrocortisone, cyclophosphamide, and methotrexate injected subcutaneously, and whole-body irradiation (500 rads) caused a reduction in the number of peritoneal cells (PE cells) obtained after intraperitoneal injection of the treated mice with proteose-peptone. The same dose of cyclophosphamide and irra...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Gadeberg OV,Rhodes JM,Larsen SO

    更新日期：1975-01-01 00:00:00

  • Characterization of a cell population in thoracic duct lymph that adoptively transfers rejection of adult Trichinella spiralis to normal rats.

    abstract：:In Trichinella spiralis-infected rats, a population of cells in thoracic duct lymph (TDL) that can adoptively transfer protection to naive rats was identified and characterized. During the course of T. spiralis infection, blast cells appeared in lymph from Day 3-4, and only Day 3-4 TDL cells had protective properties ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Bell RG,Korenaga M,Wang CH

    更新日期：1987-06-01 00:00:00

  • Trypanosoma cruzi induces suppression of DNA synthesis and inhibits expression of interleukin-2 receptors by stimulated human B lymphocytes.

    abstract：:Trypanosoma cruzi, the causative agent of Chagas' disease, suppresses immune responses during the acute phase and has been shown to induce multiple cellular alterations in activated human T lymphocytes. However, no information is available regarding the effects of this parasite on human B cells. Using an in vitro cult...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Kierszenbaum F,Moretti E,Sztein MB

    更新日期：1991-10-01 00:00:00

  • Impact of 5-aza-2'-deoxycytidine and epigallocatechin-3-gallate for induction of human regulatory T cells.

    abstract：:The epigenetic regulation of transcription factor genes is critical for T-cell lineage specification. A specific methylation pattern within a conserved region of the lineage specifying transcription factor gene FOXP3, the Treg-specific demethylated region (TSDR), is restricted to regulatory T (Treg) cells and is requi...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/imm.12261

    authors： Kehrmann J,Tatura R,Zeschnigk M,Probst-Kepper M,Geffers R,Steinmann J,Buer J

    更新日期：2014-07-01 00:00:00

  • Spontaneous priming for anti-viral envelope cytotoxic T lymphocytes in mice transgenic for a murine leukaemia virus envelope gene (Fv4).

    abstract：:Compared with non-transgenic controls, mice bearing an Fv4 murine retroviral env transgene resist infection and do not become immunosuppressed when inoculated with Friend virus (FV). When immunized with FV antigens in the absence of infectious virus, they make antibodies and cytotoxic lymphocytes (CTL) to FV comparabl...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.1997.00157.x

    authors： Nihrane A,Silver J

    更新日期：1997-02-01 00:00:00

  • Human T-cell responses to ragweed allergens: Amb V homologues.

    abstract：:Specific IgE and IgG responses to highly purified Ambrosia (ragweed) allergens, Amb a V, Amb t V and Amb p V from the artemisiifolia (short), trifida (giant) and psilostachya (western) species are strongly associated with HLA-DR2 and Dw2 (DR2.2) in allergic Caucasoid individuals. To investigate the molecular basis of ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Huang SK,Marsh DG

    更新日期：1991-07-01 00:00:00

  • Preparation of interstitial lung cells by enzymatic digestion of tissue slices: preliminary characterization by morphology and performance in functional assays.

    abstract：:A technique is reported here for the quantitative extraction of live cells from the lung interstitium; it involves the incubation of slices of perfused lung in a mixture containing optimal concentrations of collagenase, DNAse, and fetal calf serum, followed by the simultaneous recovery and fractionation of cells relea...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Holt PG,Degebrodt A,Venaille T,O'Leary C,Krska K,Flexman J,Farrell H,Shellam G,Young P,Penhale J

    更新日期：1985-01-01 00:00:00

  • H-2Dd engagement of Ly49A leads directly to Ly49A phosphorylation and recruitment of SHP1.

    abstract：:We have used a number of in vitro and in vivo techniques to identify the molecules that can bind to the cytoplasmic tail of the Ly49A receptor. Affinity chromatography using peptides corresponding to the N-terminal 18 amino acids of Ly49A allowed the recovery of a number of proteins that bound preferentially to the ty...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.1999.00825.x

    authors： Daws MR,Eriksson M,Oberg L,Ullén A,Sentman CL

    更新日期：1999-08-01 00:00:00

  • Goblet cell mucins as the selective barrier for the intestinal helminths: T-cell-independent alteration of goblet cell mucins by immunologically 'damaged' Nippostrongylus brasiliensis worms and its significance on the challenge infection with homologous a

    abstract：:The aim of this study was to examine the role of T cells on the alteration of terminal sugars of goblet cell mucins in the small intestinal mucosa of parasitized rats and to clarify the biological significance of the altered mucins in the mucosal defence against intestinal helminths. For this purpose, Nippostrongylus ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Ishikawa N,Horii Y,Oinuma T,Suganuma T,Nawa Y

    更新日期：1994-03-01 00:00:00

  • Histamine H4 receptor agonists have more activities than H4 agonism in antigen-specific human T-cell responses.

    abstract：:Histamine not only mediates immediate allergic reactions, it also regulates cellular immune responses. H4R is the most recently identified histamine receptor (HR). In the present study, we examined the in vitro effect of histamine and H4R agonists on the responses of human T cells to purified protein derivative from M...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2007.02574.x

    authors： Sugata Y,Okano M,Fujiwara T,Matsumoto R,Hattori H,Yamamoto M,Nishibori M,Nishizaki K

    更新日期：2007-06-01 00:00:00

  • Hepatic uptake of small-latticed immune complexes does not alter mononuclear phagocyte system function.

    abstract：:The hepatic and splenic uptake of circulating, small-latticed immune complexes and the effect of these complexes on the hepatic mononuclear phagocyte system (MPS) were examined in mice. The small-latticed immune complexes were prepared at fifty-fold antigen excess. The clearance from circulation and uptake by the live...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Jimenez RA,Haakenstad AO,Mannik M

    更新日期：1983-02-01 00:00:00

  • Effect of oestradiol and pathogen-associated molecular patterns on class II-mediated antigen presentation and immunomodulatory molecule expression in the mouse female reproductive tract.

    abstract：:Cells of the female reproductive tract (FRT) can present antigen to naive and memory T cells. However, the effects of oestrogen, known to modulate immune responses, on antigen presentation in the FRT remain undefined. In the present study, DO11.10 T-cell antigen receptor transgenic mice specific for the class II MHC-r...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2011.03512.x

    authors： Ochiel DO,Rossoll RM,Schaefer TM,Wira CR

    更新日期：2012-01-01 00:00:00