Abstract:
:We have used a number of in vitro and in vivo techniques to identify the molecules that can bind to the cytoplasmic tail of the Ly49A receptor. Affinity chromatography using peptides corresponding to the N-terminal 18 amino acids of Ly49A allowed the recovery of a number of proteins that bound preferentially to the tyrosine-phosphorylated peptide, including SH2-containing phosphatase-1 (SHP1) and the SH2-containing inositol 5' phosphatase (SHIP). In another approach, using the entire cytoplasmic domain of the Ly49A receptor, we found that SHP2 also interacted with the tyrosine-phosphorylated form of the Ly49A cytoplasmic tail. Using BIACORE(R)2000 analysis, we determined that both SHP1 and SHP2 bound to the tyrosine-phosphorylated cytoplasmic tail of Ly49A with affinities in the nanomolar range, whilst SHIP showed no binding. Mutation of tyrosine-36 to phenylalanine did not significantly affect the affinities of these proteins for the tyrosine-phosphorylated cytoplasmic tail of Ly49A. In addition, using a whole-cell system with T-cell lymphoma cell lines that expressed the Ly49A receptor or its H-2Dd ligand, we determined that engagement of Ly49A by its major histocompatibility complex (MHC) ligand leads to tyrosine-phosphorylation events and recruitment of SHP1. Recruitment of SHP1 was rapid and transient, reaching a maximum after 5 min. These data suggest that mechanisms for the inhibitory signal are generated following receptor engagement. They also provide direct evidence that ligand engagement of the Ly49A receptor is responsible for recruitment of downstream signalling molecules.
journal_name
Immunologyjournal_title
Immunologyauthors
Daws MR,Eriksson M,Oberg L,Ullén A,Sentman CLdoi
10.1046/j.1365-2567.1999.00825.xkeywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
656-64issue
4eissn
0019-2805issn
1365-2567pii
imm825journal_volume
97pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Signal transducers and activators of transcription (STATs) are key molecular determinants of T-cell fate and effector function. Several inflammatory diseases are characterized by an altered balance of T-cell phenotypes and cytokine secretion. STATs, therefore, represent viable therapeutic targets in numerous pathologi...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12851
更新日期:2018-04-01 00:00:00
abstract::MUC1 is a transmembrane mucin that is expressed on ductal epithelial cells and epithelial malignancies and has been proposed as a target antigen for immunotherapy. The expression of MUC1 has recently been reported on T and B cells. In this study we demonstrate that following activation in vivo or activation by differe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01562.x
更新日期:2003-01-01 00:00:00
abstract::It has previously been shown in mice that activated peritoneal macrophages, when first removed from the peritoneal cavity, suppress mitogen-stimulated lymphocyte transformation in vitro as measured by [3H]-thymidine incorporation. In this study, it is demonstrated that activated macrophages that have been incubated al...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::The influence of antigens of the major histocompatibility complex (MHC) on the production of interferon (IFN) alpha or IFN gamma by human peripheral blood leucocytes (PBL) in vitro has been studied. Synthesis of IFN gamma by PBL stimulated with purified phytohaemagglutinin (PHA-P) or protein A of Staphylococcus aureus...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract::The influence of the synthetic corticosteroid dexamethasone sodium phosphate (DEXA) upon mouse B cells was studied. This was done by in vivo treatment of mice with a single or multiple injection of DEXA, and by culturing murine spleen cells and bone marrow cells in vitro in the presence of different concentrations of ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::Murine lymphokine-activated killer (LAK) cells induced by systemic high-dose recombinant human interleukin 2 (IL-2) administration lysed fresh syngeneic peritoneal macrophages (M phi). LAK cells lysed resident peritoneal M phi and M phi activated in vivo with thioglycollate (TG), Corynebacterium parvum (C. parvum), or...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::Crohn's disease (CD) is a chronic inflammatory disease of the gastrointestinal tract. Its pathogenesis is not completely understood, though the prevailing model is that the intestinal flora drives a strong intestinal T helper 1 (Th1)/Th17 type immune response and inflammation in the genetically susceptible host. This ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02839.x
更新日期:2008-10-01 00:00:00
abstract::To further investigate the immunomodulatory effects of dietary lipids, rats were fed on a low-fat diet or on high-fat diets that contained hydrogenated coconut, olive, safflower, evening primrose or fish oil as the principal fat source. The fish oil diet decreased the level of expression of CD2, CD11a, CD18 and CD44 o...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00474.x
更新日期:1998-05-01 00:00:00
abstract::Following exposure of mice to contact sensitizing chemicals, dendritic cells (DC) rapidly accumulate in the draining lymph nodes. A proportion, at least, of the DC which arrive in the nodes bear significant amounts of antigen and are derived from epidermal Langerhans' cells (LC). It is of interest that although LC are...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::Lipoprotein lipase (LPL) is the key enzyme in the metabolism of triglyceride-rich lipoproteins. The patterns of LPL mRNA expression and secretion of the enzyme have not yet been established in natural killer (NK) cells. We show in the present communication that CD3- CD16+ cells (NK cells) transcribe LPL mRNA, express ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Successful elimination of the hepatitis C virus (HCV) during acute infection has been linked to strong HCV-specific in vitro T-cell proliferation, whereas T cells from patients with chronic hepatitis C respond only weakly to HCV antigens. Lipid-coupled peptides are immunostimulants, which might provide a basis for nov...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01209.x
更新日期:2001-04-01 00:00:00
abstract::One of the hallmarks of systemic autoimmune diseases, such as systemic lupus erythematosus (SLE), is the immune response to nuclear autoantigens. Several studies have proposed that dendritic cells may acquire the nuclear autoantigens from the apoptotic cells to initiate the systemic autoimmune responses. To examine th...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01438.x
更新日期:2002-07-01 00:00:00
abstract::Experiments were conducted to test the hypothesis that opsonic and non-opsonic phagocytic capacities are differentially regulated by resting and wound-derived macrophages. Furthermore, the phagocytosis of non-opsonized zymosan and beta-glucan particles was quantified to determine whether cells differentially regulate ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01291.x
更新日期:2001-10-01 00:00:00
abstract::The gut microbiota provides an important stimulus for the induction of regulatory T (Treg) cells in mice, whether this applies to newborn children is unknown. In Swedish children, Staphylococcus aureus has become a common early colonizer of the gut. Here, we sought to study the effects of bacterial stimulation on neon...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12209
更新日期:2014-03-01 00:00:00
abstract::Activation of self-reactive T cells in healthy adults is prevented by the presence of autoantigen-specific CD4+CD25+ regulatory T cells (CD25+ Treg). To explore the functional development of autoantigen-reactive CD25+ Treg in humans we investigated if thymic CD25+ Treg from children aged 2 months to 11 years and cord ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02186.x
更新日期:2005-08-01 00:00:00
abstract::The in vitro frequency and proliferative capacity of B cells and B-cell precursors in old mice was assessed. The mitogenic response to the B-cell mitogens lipopolysaccharide (LPS) and dextran sulphate decline later and at a slower rate than the T-cell response to phytohaemagglutinin and concanavalin A. The response to...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Antibody-dependent cellular cytotoxicity (ADCC) and Fc receptor-mediated phagocytosis were determined with human monocytes, monocyte-derived macrophages and activated macrophages, using rabbit IgG-covered sheep red blood cells (EAs) and anti-D-treated human erythrocytes (EAhu) as target cells. Monocyte and macrophage-...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::In this report the antigen and restriction specificity of human T-cell clones induced with Dermatophagoides farinae (D. farinae) and isolated from an atopic individual with perennial rhinitis has been investigated. Of the six clones analysed, four were species specific and two showed cross-reactivity for the closely r...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::The requirement for activity of the enzyme ADP-ribosyl transferase (ADPRT) and changes in single-strand DNA breaks were assessed during the initial stimulation of quiescent murine splenic lymphocytes with mitogen alone, the stimulation of activated blasts with IL-2-containing medium and, for comparison, the serum stim...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-08-01 00:00:00
abstract::We established in previous studies that the binding of Salmonella lipopolysaccharide (LPS) to constitutive receptors of low affinity triggers the expression of the inducible LPS-binding molecule CD14 in bone marrow cells (BMC) of C3H/HeOU mice, but not in BMC from C3H/HeJ mice. We show in this study that BMC from C3H/...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00107.x
更新日期:2000-10-01 00:00:00
abstract::Undesired immunological responses to products of therapeutic gene replacement have been obstacles to successful gene therapy. Understanding such responses of the host immune system to achieve immunological tolerance to a transferred gene product is therefore crucial. In this article, we review relevant studies of immu...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2009.03069.x
更新日期:2009-05-01 00:00:00
abstract::Haemolymph of the American lobster, Homarus americanus, contains several lectins. One of these, lobster agglutinin 1 (LAg1) is specific for N-acetylneuraminic acid and agglutinates mouse and human erythrocytes. In addition, this lectin agglutinates peripheral T cells and cortisone-resistant thymocytes, but agglutinate...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::The results presented in this paper demonstrate that a mouse IgM monoclonal antibody (T-80) recognizes an antigen on cells of the T-lymphocyte lineage of sheep. However, this antibody does not identify all T cells, as 10-20% of thymocytes and some peripheral-blood T cells are negative. T-80- thymocytes reside in the m...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract::This study describes a subtractive hybridization analysis to identify differences in gene expression between sibling Th memory clones, elicited by virus infection and expressing identical T-cell receptor (TCR)-alphabeta rearrangements but distinct lymphokine phenotype: clone Bpp9 secretes interleukin (IL)-4, IL-5 and ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01953.x
更新日期:2004-10-01 00:00:00
abstract::Gene ablation studies in mice indicate that lymphotoxin (LT)alpha, LTbeta and LTbetaR are essential for the genesis of lymph nodes (LN), normal structural development of peripheral lymphoid tissues and the differentiation of natural killer (NK) cells. LTbetaR binds to the heterotrimeric cytokines LTalpha1beta2 and LIG...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01586.x
更新日期:2003-03-01 00:00:00
abstract::Major histocompatibility complex (MHC) alleles acting as immune response genes are coexpressed in heterozygous individuals and therefore control of immune responses is usually codominant. As an exception to this rule, however, several examples of recessive immune responses have been ascribed to regulatory, e.g. suppre...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00956.x
更新日期:2000-02-01 00:00:00
abstract::Chlamydia trachomatis is a major cause of sexually transmitted disease, infertility and reactive arthritis in the Western world, and of trachoma in the developing world. There is evidence that the chronic inflammatory reaction seen in diseases associated with chlamydiae represents a delayed-type hypersensitivity respo...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::In tumour (fibrosarcoma)-inoculated mice, alterations of host resistance to Listeria monocytogenes following tumour growth were examined. Non-immune macrophage-mediated antibacterial resistance was severely suppressed up to day 4 or so after tumour inoculation, but was enhanced thereafter. On the other hand, T cell-me...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Goats were immunized with membrane fractions of the L5178Y tumour syngenic to DBA/2 mice. IgG fractions of this antiserum were made tumour specific by repeated in vitro and in vivo absorptions with normal cells and concentrated by adsorption to and elution from tumour cells. In vivo studies indicated that the accumula...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::Vaccination strategies that can block or limit heterosexual human immunodeficiency virus (HIV) transmissions to local and systemic tissues are the goal of much research effort. Herein, in a mouse model, we aimed to determine whether the enhancement of antibody responses through mucosal and systemic immunizations, prev...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2007.02702.x
更新日期:2008-03-01 00:00:00