Abstract:
:Crohn's disease (CD) is a chronic inflammatory disease of the gastrointestinal tract. Its pathogenesis is not completely understood, though the prevailing model is that the intestinal flora drives a strong intestinal T helper 1 (Th1)/Th17 type immune response and inflammation in the genetically susceptible host. This leads to overly aggressive T-cell responses to normal bacteria causing tissue damage. Intestinal homeostasis and maintenance of tolerance to harmless antigens in the intestine has been shown to be maintained by CD4+ CD25+ T regulatory cells (Treg) in animal models of inflammatory bowel diseases. Here we investigated whether Infliximab, a chimeric monoclonal antibody directed against tumour necrosis factor (TNF)-alpha shown to be highly effective in the treatment of CD, has any effect on mucosal CD4+ CD25+ (FOXP3+) Tregs. Colonic mucosal biopsies from children with active Crohn's disease treated in vivo with Infliximab and controls were analysed to determine FOXP3 expression by immunofluorescence and reverse transcription-polymerase chain reaction. We observed that FOXP3+ T cells were significantly reduced in mucosa of CD patients with active disease compared with controls and restored to normal following Infliximab treatment. The frequency of FOXP3+ cells and mRNA expression was significantly increased in CD mucosa from patients treated in vivo with Infliximab compared with CD patients treated with conventional therapies. In conclusion, we show that Infliximab treatment does not solely neutralize soluble TNF-alpha, but also affects activation and possibly expansion of mucosal regulatory T cells. We suggest that anti TNF-alpha immunotherapy can also restore mucosal homeostasis in Crohn's disease.
journal_name
Immunologyjournal_title
Immunologyauthors
Ricciardelli I,Lindley KJ,Londei M,Quaratino Sdoi
10.1111/j.1365-2567.2008.02839.xsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
178-83issue
2eissn
0019-2805issn
1365-2567pii
IMM2839journal_volume
125pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Selective Alzheimer disease indicator-1 (seladin-1) is a broadly expressed oxidoreductase and is related to Alzheimer disease, cholesterol metabolism and carcinogenesis. The effect of lipopolysaccharide (LPS) on the expression of seladin-1 was examined using RAW 264.7 macrophage-like cells and murine peritoneal macrop...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03274.x
更新日期:2010-09-01 00:00:00
abstract::Forssman antiserum produced in rabbits immunized with sheep erythrocyte stromata was found to contain an IgG antibody which inhibited both passive anaphylactic sensitization of guinea-pig lung and also histamine-releasing activity of soluble immune complexes. This Forssman antibody did not itself cause histamine rele...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) catalyse the formation of hypochlorite (OCl-) from chloride ions (OCl-) and hydrogen peroxide (H2O2). OCl- proved to be highly toxic for Trichinella spiralis newborn larvae (NBL) in in vitro assays. Using purified human MPO and EPO it was found that even at neutral...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::To determine the influence of anti-idiotypic antibodies on the complementary idiotype (Id), the clearance kinetics, tissue distribution and fate of idiotype-anti-idiotype complexes were investigated in mice. The complexes were prepared by mixing purified radiolabelled monomeric (mIgA) or polymeric IgA (pIgA) with mono...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::The ultimate outcome of T cell receptor recognition is determined by the context in which the antigen is encountered. In this fashion both antigen-presenting cells and T cells must integrate multiple environmental cues in the form of pathogen-associated molecular patterns, cytokines and accessory molecule signals. The...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2009.03125.x
更新日期:2009-08-01 00:00:00
abstract::It has been demonstrated that the single autosomal recessive lpr and gld genes are responsible for the accumulation of unusual T-cell subsets. Although these subsets have been assigned to the T-cell lineage, they share certain antigenic cell surface markers with mature B lymphocytes. Consequently the maturational path...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::The specific recognition of the acetylcholine receptor and its alpha-subunit by T cells derived from patients with myasthenia gravis or mice with experimental autoimmune myasthenia gravis raises the question of the role of autoreactive T cells in the myasthenic process. Sequences of the acetylcholine receptor alpha-su...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Silica, or silicon dioxide, has been shown to be toxic for macrophages. This is probably because it damages phagolysosomal membranes, allowing lysosomal enzymes to disrupt the cell. Neutrophils also take up particles such as silica and in addition they contain lysosomes. The purpose of this study was to determine whet...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::A common factor existing among autoimmune target antigens was sought in association with their developmental expression during organogenesis. Autoimmunity against a certain organ was experimentally induced in rats by deliberate immunization with whole tissue extract of the respective organ. Histopathological changes i...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::Natural cell-mediated cytotoxicity is mediated by a family of effector cells that express cytolytic activities distinct from those generally attributed to B cells, T cells and macrophages; it includes both natural killer (NK) and natural cytotoxic (NC) activities. There is now convincing evidence to show that NC activ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is an important molecule in the down-regulation of T-cell activation. A study was undertaken to evaluate the association of the CTLA-4 gene polymorphisms -319C/T, +49A/G, (AT)(n), CT60A/G and Jo31G/T with the levels of membrane CTLA-4 (mCTLA-4) and cytoplasmic CTLA-4 (cCTLA-4)...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03083.x
更新日期:2009-09-01 00:00:00
abstract::The class distribution of 4193 monoclonal gammopathies (MG), detected over approximately 10 years in western France (WF), was compared to that established by a compilation of twenty-seven series from the literature. Several points emerged from this study: the incidence of the light chain MG (4.8%) was slightly lower t...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::It has been reported previously that environmental antigens such as intestinal microflora play an important role in an age-associated increase in number of T-cell receptor gamma/delta-bearing T cells. To extend the scope of this finding, this study examined the influences of local inflammation on the accumulation and/...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::This study examines the expression of mRNA for the Th2 cytokine, interleukin-4 (IL-4). Peripheral blood mononuclear cells from deer infected with Mycobacterium bovis or vaccinated with live or killed M. bovis bacillus Calmette-Guérin (BCG) were cultured with mycobacterial antigens. IL-4 mRNA production was assayed usi...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.1996.tb00014.x
更新日期:1996-06-01 00:00:00
abstract::Although interleukin-21 (IL-21) potently activates and controls the differentiation of immune cells after stimulation in vitro, the role for this pleiotropic cytokine during in vivo infection remains poorly defined. Herein, the requirement for IL-21 in innate and adaptive host defence after Listeria monocytogenes infe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03287.x
更新日期:2010-10-01 00:00:00
abstract::The Mycobacterium leprae and M. tuberculosis 10,000 MW heat-shock protein homologues of GroES have previously been identified as major immunogens for human T cells. We used synthetic peptides to characterize the determinants recognized by murine T cells. The findings suggest that, despite 90% sequence identity between...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00400.x
更新日期:1998-01-01 00:00:00
abstract::Leukosialin or CD43 is a heavily O-glycosylated transmembrane protein expressed on all cells of the haematopoietic cell lineage with the exception of red blood cells and mature B cells. This antigen has been identified in human, mouse and rat with monoclonal antibodies. Although orthologues of many human and rodent le...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00604.x
更新日期:1999-01-01 00:00:00
abstract::Synthesis of interferon (IFN)-gamma by natural killer (NK) cells is an important pro-inflammatory event with interleukin (IL)-12 and IL-18 playing major inductive roles. However, other temporal events are likely to regulate such processes and as prostaglandin E2 (PGE2) is ubiquitous during inflammation this study test...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01810.x
更新日期:2004-03-01 00:00:00
abstract::In addition to macromolecular interactions that provide co-stimulation during antigen-presenting cell (APC) and CD4+ T-cell conjugation, covalent chemical events between specialized ligands have been implicated in T-cell co-stimulation. These take the form of transient Schiff base formation between carbonyls and amine...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01290.x
更新日期:2001-09-01 00:00:00
abstract::Culture dishes precoated with thin layers of acid soluble rat tail collagen simplify conditions necessary to obtain in vitro high IgG anti-DNP responses from primed and boosted mice. In contrast to Mishell-Dutton type cultures the use of collagen precludes the need to continually rock cultures and to add nutrients. Re...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::The activation of guinea-pig eosinophils was studied by measuring the production of superoxide anion (O2-) and the secretion of eosinophil peroxidase (EPO). Phorbol myristate acetate (PMA), calcium ionophore, plasma-activated zymosan, concanavalin A and recombinant human anaphylatoxin C5a induced the release of varyin...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::Interleukin-15 (IL-15) is a proinflammatory cytokine that is overexpressed in rheumatoid arthritis (RA), a disease characterized by activation of monocytes/macrophages (MPhi), and by expansion of autoreactive CD4(+) T cells. We hypothesized that IL-15 plays a major role for this expansion of CD4(+) T cells and modulat...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02878.x
更新日期:2009-01-01 00:00:00
abstract::Mice infected by intraperitoneal injection of Mycobacterium tuberculosis were studied over a 23-week period. They showed progressive infection in the lung (with increasing microbial count and granuloma size) whereas viable bacillary counts remained largely stationary in the spleen and in the liver. The influence of H-...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::Interleukin-2 (IL-2) is a critical regulator of immune homeostasis through its non-redundant role in regulatory T (Treg) cell biology. There is major interest in therapeutic modulation of the IL-2 pathway to promote immune activation in the context of tumour immunotherapy or to enhance immune suppression in the contex...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12609
更新日期:2016-07-01 00:00:00
abstract::Dendritic cells (DC) are the initiators of immune responses and are present in most tissues in vivo. To generate myeloid DC from monocytes (MoDC) in vitro the necessary cytokines are granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Using degenerated primers delineated from other spec...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02319.x
更新日期:2006-04-01 00:00:00
abstract::The molecular characteristics of a serum IL-2 inhibitor were determined by fractionating active sera from normal and malaria-infected mice, and assaying inhibitory activity in blocking production and expression of IL-2 activity. Using isoelectric focusing and ion exchange chromatography, the activity resolved in a sin...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::The spontaneous regression of AK-5 histiocytoma is mediated by natural killer (NK) cells through antibody-dependent cell-mediated cellular cytotoxicity (ADCC) and the target cell death involves necrosis and apoptosis. We have studied the NK cell activation and the associated induction of apoptosis in the AK-5 tumour i...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Goats were immunized with membrane fractions of the L5178Y tumour syngenic to DBA/2 mice. IgG fractions of this antiserum were made tumour specific by repeated in vitro and in vivo absorptions with normal cells and concentrated by adsorption to and elution from tumour cells. In vivo studies indicated that the accumula...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::Gene transfer to create tumour epitope-specific cytolytic T cells for adoptive immunotherapy of cancer remains an area of active inquiry. When the Mart-127-35 -specific DMF5 T-cell receptor (TCR) is transferred into peripheral human CD4(+) T cells, the reprogrammed cells exhibit a T helper type 1 (Th1) phenotype with ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12431
更新日期:2015-05-01 00:00:00
abstract::Activation of human eosinophils by platelet-activating factor (PAF) involves multiple signal transduction pathways. Among these, protein kinase C has been demonstrated both to mediate respiratory burst and to suppress an alternative pathway of activation of respiratory burst and arachidonic acid metabolism in eosinoph...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00037.x
更新日期:2000-06-01 00:00:00