Abstract：
:Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) catalyse the formation of hypochlorite (OCl-) from chloride ions (OCl-) and hydrogen peroxide (H2O2). OCl- proved to be highly toxic for Trichinella spiralis newborn larvae (NBL) in in vitro assays. Using purified human MPO and EPO it was found that even at neutral pH both enzymes under appropriate conditions are able to kill NBL. The rate at which OCl- is produced is much lower in the EPO- than in the MPO-mediated reaction. This difference in enzymic activity may explain why in the MPO-mediated reaction half the amount of OCl- was sufficient to kill 50% of the NBL, as compared to the EPO-mediated reaction. Purified human eosinophil major basic protein showed excellent OCl- scavenging properties, resulting in a significant decrease in the EPO-mediated NBL killing. Addition of ammonium ions [(NH4)2SO4] to the EPO-mediated reaction increased the NBL killing remarkably. It was concluded that in vitro MPO is more efficient than EPO in killing NBL. Furthermore, it was suggested that although eosinophils show marked parasiticidal effects in various in vitro systems, their primary biological role might be the regulation of the inflammatory reactions.
journal_name
Immunologyjournal_title
Immunologyauthors
Buys J,Wever R,Ruitenberg EJsubject
Has Abstractpub_date
1984-03-01 00:00:00pages
601-7issue
3eissn
0019-2805issn
1365-2567journal_volume
51pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:The decay-accelerating factor (DAF) contains four complement control protein repeats (CCPs) with a single N-linked glycan positioned between CCPs 1 and 2. In previous studies we found that the classical pathway regulatory activity of DAF resides in CCPs 2 and 3 while its alternative pathway regulatory activity resides...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00086.x
更新日期：2000-09-01 00:00:00
abstract：:The tumour microenvironment is complex containing not only neoplastic cells but also a variety of host cells. The heterogeneous infiltrating immune cells include subsets of cells with opposing functions, whose activities are mediated either directly or through the cytokines they produce. Systemic delivery of cytokines...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12037
更新日期：2013-03-01 00:00:00
abstract：:Although it is clear that the CD45 tyrosine phosphatase is required for efficient T-cell activation and T-cell development, the factors that regulate CD45 function remain uncertain. Previous data have indicated that there is an association of CD45 with CD4 and the T-cell receptor (TCR) complex controlled by the variab...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02602.x
更新日期：2007-08-01 00:00:00
abstract：:The mechanisms of action and the nature of the co-operating cells (CC) controlling human T-lymphocyte-colony formation were investigated. Media conditioned by PHA-stimulated blood mononuclear cells (MC) were tested for their capacity to induce T-colony formation in the effluent cell population, obtained after anti (Fa...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:Rabbit spleen and mesenteric lymph node cells were treated with a monoclonal anti-rabbit T-lymphocyte antibody (MAb) and complement and the effect of the treatment on various lymphocyte functions was determined. Lysis of spleen and mesenteric lymph node cells reactive with this MAb, 9AE10, essentially eliminated their...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:The molecular mechanisms underlying B- and T-cell development are, as yet, poorly understood. However, as G proteins regulate a diverse range of biological responses including growth, proliferation and differentiation, we have investigated differential expression of G proteins during B- and T-cell development with the...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00196.x
更新日期：1997-04-01 00:00:00
abstract：:The classical acute phase reactant, C-reactive protein (CRP), appears in markedly elevated concentration in the sera of individuals undergoing reactions of acute inflammation and tissue degradation. We previously demonstrated that like IgG, appropriately purified CRP could be thermally modified (H-CRP) such that it en...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:Class switch recombination (CSR) is a T-cell-dependent mechanism regulating isotype switching in activated mature B cells. Recently we showed that T-cell-independent CSRs occur spontaneously during B lymphopoiesis, but such cells are negatively selected by Fas signalling. In immunoglobulin mu-deficient mice, lack of F...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2004.01883.x
更新日期：2004-06-01 00:00:00
abstract：:Blood dendritic cells carry the terminal complement complex (TCC) on their surface, as detected by three monoclonal antibodies (mAb). Two of these mAb were generated by immunizing mice with the terminal complement complex, whereas the third was generated by immunizing mice with blood dendritic cells. All three mAb are...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:Intraepithelial lymphocytes (IEL) in normal human small intestine exhibit cytotoxicity. This study was undertaken to characterize the effector cells and their mode of action. Freshly isolated jejunal IEL and lamina propria lymphocytes (LPL), as well as IEL and LPL depleted of CD4+, CD8+ and T-cell receptor (TCR)-gamma...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2002.01461.x
更新日期：2002-08-01 00:00:00
abstract：:Immununoelectron microscopy of human peripheral blood mononuclear cells enriched for the presence of antigen-presenting dendritic cells (DC) has revealed two morphologically distinct cell types both expressing DR and DQ major histocompatibility complex (MHC) class II antigens but lacking T, B, natural killer (NK) and ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:Evidence suggests that the immune and neuroendocrine systems cross talk by sharing ligands and receptors. Hormones and neuropeptides produced by the neuroendocrine system often modulate the function of lymphoid organs and immune cells. We have previously reported the intrathymic expression of somatostatin (SOM) in the...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2006.02351.x
更新日期：2006-05-01 00:00:00
abstract：:Virus-specific cytotoxic T-cell (Tc) clones can lyse target cells in vitro in the presence of their specific peptide epitopes. The lytic potency of murine influenza nucleoprotein (NP)-specific Tc clones was investigated after observing that target cell killing was reduced in the presence of high (greater than 0.2 micr...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:The plant lectin concanavalin A (Con A) has been used in an invertebrate model of lectin-dependent cell-mediated cytotoxicity (LDCC). Macrophage-like cells from the susceptible host snail Biomphalaria glabrata become cytotoxic effectors when they encounter sporocysts of the parasitic trematode Schistosoma mansoni that...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:We have identified two components of human seminal plasma which suppress natural killer (NK) cell activity in vitro. Dialysis and gel filtration experiments have shown both components to be of low molecular weight. The first will suppress NK cells following a short period of pretreatment, but this suppression is depen...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:The molecular characteristics of a serum IL-2 inhibitor were determined by fractionating active sera from normal and malaria-infected mice, and assaying inhibitory activity in blocking production and expression of IL-2 activity. Using isoelectric focusing and ion exchange chromatography, the activity resolved in a sin...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:The aim of this study was to characterize the epitope specificity and the idiotype(s) of gp330-specific autoantibodies in active Heymann nephritis (HN). To study this, two monoclonal autoantibodies (mAb), 3D9B and 5B8C, derived from Lewis rats with HN, were generated. These antibodies bound to different epitopes on th...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
abstract：:The IgG and IgA subclass distribution of anti-carbohydrate antibodies in normal and immunodeficient donors was investigated. In normal donors, the specific anti-dextran antibodies were mainly of the IgG2 and IgA2 subclass, although substantial amounts of antibodies could also be of the IgG1 subclass. In children, IgG1...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:The intestinal lamina propria (LP) contains antigen-presenting cells with features of dendritic cells and macrophages, collectively referred to as mononuclear phagocytes (MNPs). Association of MNPs with the epithelium is thought to play an important role in multiple facets of intestinal immunity including imprinting M...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12801
更新日期：2017-12-01 00:00:00
abstract：:Haptoglobin (Hp), a major acute-phase plasma protein, has been found in arthritic synovial fluid (SF). However, the function and structural modifications of Hp in arthritic SF are unknown. To investigate in vivo generation of modified Hp associated with inflammatory disease, we examined a new Hp isoform in SF from pat...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12125
更新日期：2013-09-01 00:00:00
abstract：:The effects of a variety of agents on the mouse antibody response to sheep red cells (SRC) and Escherichia coli lipopolysaccharide (LPS) were investigated. Agents formed two broad groups according to the time-dependence of their effects. Class I agents inhibited responses if given at any time over a broad period from ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
abstract：:The interaction of mouse intestinal mast cell proteinase (IMCP) with serine proteinase inhibitors (serpins) in blood was analysed: (i) by examining the capacity of the inhibitors in blood to block the binding of the irreversible serine esterase inhibitor [3H]diisopropyl fluorophosphate (DFP); (ii) by Western blotting....
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Immunoglobulin D (IgD) is normally present in only low concentrations in serum. In the hyper-IgD and periodic fever syndrome (HIDS), however, serum levels exceed 140 mg/l. This syndrome is further characterized by recurrent inflammatory febrile attacks together with an acute phase response and appearance of cytokines ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1996.d01-672.x
更新日期：1996-07-01 00:00:00
abstract：:We previously identified a cis-acting element within the 3' untranslated region of CD40 ligand messenger RNA (mRNA) that is composed of three complex binding sites and acts to increase mRNA stability in both in vitro and in vivo systems. We now demonstrate the functional consequences of the three binding sites with re...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02783.x
更新日期：2008-07-01 00:00:00
abstract：:In order to elucidate possible roles of thymic nurse cells (TNC) we isolated them as lympho-epithelial cell complexes from spontaneous thymomas of BUF/Mna rats and characterized them using immuno- and enzyme-histochemical techniques. A remarkable increase in the number of TNC was seen at 8 months of age, immediately b...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Ribonucleic acid (immune RNA, iRNA), extracted from the spleens of mice immunized with sheep red blood cells (SRBC), induced specific immunological memory. The humoral immune responses of iRNA-treated animals were compared with in vivo and in vitro primary and secondary responses, using the haemolytic plaque-forming c...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:The absence of complement receptor 1 (CR1) related gene/protein y (Crry) leads to embryonic lethality as a result of unrestricted complement activation and concomitant neutrophil infiltration. Here we used Crry(-/-)C3(+/-) mice to investigate the role of Crry in the pathogenesis of immune complex glomerulonephritis (G...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2009.03102.x
更新日期：2009-09-01 00:00:00
abstract：:Young (15-week-old) CBA/Ca mice were injected intravenously with spleen cells from individual young (15-week-old) or old (18-24-month-old) CBA/T6T6 mice. Samples of peripheral blood were taken at monthly intervals and cultured with phytohaemagglutinin (PHA) to stimulate T lymphocytes into mitosis. In the recipients of...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:When cultured on a collagen matrix at a density of 7 X 10(4) cells/cm2 for 48 hr, 80 +/- 10% of unfractionated and 70 +/- 14% of T-enriched human blood lymphocytes from eight healthy individuals developed a motile morphology defined as the presence of lamellar surface activity and cytoplasmic flattening. During cultur...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:A rat thymic epithelial cell (TEC) line (R-TNC.1) was established from a long-term TEC culture. Based on its ultrastructure, phenotype and cytokeratin profile, this line was characterized as a type of cortical TEC. R-TNC.1 cells had nursing activity which was manifested by the binding and subsequent engulfment of thym...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
