Abstract:
:To determine the influence of anti-idiotypic antibodies on the complementary idiotype (Id), the clearance kinetics, tissue distribution and fate of idiotype-anti-idiotype complexes were investigated in mice. The complexes were prepared by mixing purified radiolabelled monomeric (mIgA) or polymeric IgA (pIgA) with monoclonal anti-T15 idiotype (B39-38). The 24 hr clearance from circulation of intravenously administered mIgA, mIgA-anti-Id complexes, pIgA and pIgA-anti-Id complexes showed three exponential phases. There was no significant difference in the amount, half-life (t 1/2), or organ distribution of mIgA and mIgA-anti-Id during the first two phases. The mIgA-anti-Id complexes were removed at a faster rate (9.2 +/- 0.5 hr) than the mIgA alone (17.7 +/- 1.3 hr) during the third phase. In contrast, anti-Id affected the clearance of pIgA during the first phase whereby 75% of the administered pIgA-anti-Id complexes, compared with 50% of the pIgA, cleared from the circulation with a t 1/2 of 3 min. This rapid removal from circulation was mediated predominantly by the liver, where 66% of pIgA-anti-Id and 40% of pIgA were detected 10 min after administration of the radiolabelled material. In the second phase, pIgA-anti-Id were removed from circulation at a faster rate (t 1/2 = 28 min) than pIgA (t 1/2 = 43 min). During this phase, 1 hr after administration of radiolabelled material, significantly more pIgA-anti-Id than pIgA were localized in the liver, kidneys, skin and spleen. In contrast, the digestive tract contained more pIgA (22%) than pIgA-anti-Id (11%). Analysis of the mIgA-anti-Id and pIgA-anti-Id by gradient polyacrylamide gel electrophoresis indicated that mIgA-anti-Id were composed of small-sized complexes, while intermediate-sized complexes constituted the majority of the pIgA-anti-Id complexes. These results suggest that the modulatory effect of anti-Id on the complementary IgA idiotype is determined largely by the molecular form of the IgA and the size of the Id-anti-Id complexes.
journal_name
Immunologyjournal_title
Immunologyauthors
Rifai A,Wu CGsubject
Has Abstractpub_date
1990-04-01 00:00:00pages
610-5issue
4eissn
0019-2805issn
1365-2567journal_volume
69pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Chicken antibodies (immunoglobulin Y; IgY) to the alpha Gal epitope (galactose alpha-1,3-galactose) bind to alpha Gal antigens of mouse and porcine tissues and endothelial cells in vitro and block human anti-alpha Gal antibody binding, complement activation and antibody-dependent cell-mediated lysis mechanisms. The ac...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00136.x
更新日期:2000-12-01 00:00:00
abstract::Despite the capacity for antigen-specific activation and rapid clonal expansion, homeostatic mechanisms ensure that the mature immune system contains a relatively stable number of T cells. In recent years, it has become apparent that this stability is a consequence of apoptotic death of most of the specific T cells ge...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00048.x
更新日期:2000-07-01 00:00:00
abstract::Trypanosoma cruzi, the causative agent of Chagas' disease, is a protozoan parasite that infects humans and other mammals in Central and Latin America. Several alterations of the immune response after infection have been described, such as severe immunosuppression of both cellular and humoral responses and massive poly...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00498.x
更新日期:1998-06-01 00:00:00
abstract::Clonal deletion of T cells capable of recognizing both host-type Mls and donor-type Mls occurred in the peripheral mature T-cell pool in radiation bone marrow chimeras of two H-2-compatible Mls-disparate strain combinations of AKR/J(H-2k,Thy-1.1,Mls-1a) and C3H/He(H-2k,Thy-1.2,Mls-1b). In order to determine further th...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::The specificities of antibodies raised in rabbits against rat mast cell proteinase 1 (RMCP 1) from connective tissue mast cells (CTMC), and against RMCP II from mucosal mast cells (MMC), were analysed by SDS-PAGE and Western blotting. Significant cross-reactivity was detected, and was eliminated by affinity purificati...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::Among their diverse roles as transcriptional regulators during development and cell fate specification, the RUNX transcription factors are best known for the parts they play in haematopoiesis. RUNX proteins are expressed throughout all haematopoietic lineages, being necessary for the emergence of the first haematopoie...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12535
更新日期:2015-12-01 00:00:00
abstract::This study addresses the question of whether human peripheral CD4+ CD45RA+ T cells possess antigen-specific immune memory. CD4+ CD45RA+ T cells were isolated by a combination of positive and negative selection. Putative CD4+ CD45RA+ cells expressed CD45RA (98.9%) and contained < 0.1% CD4+ CD45RO+ and < 0.5% CD4+ CD45R...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00274.x
更新日期:1997-07-01 00:00:00
abstract::Epididymitis represents a serious threat to male fertility and usually develops following secondary bacterial infection of the epididymis such as urinary tract infections or sexually transmitted diseases. Surprisingly, very little is known about the innate host response triggered by bacterial infection in the male rep...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2007.02763.x
更新日期:2008-06-01 00:00:00
abstract::The CTLL-2 bioassay is used frequently to determine interleukin-2 (IL-2) concentrations in experimental samples, including samples that contain reagents which affect the CD28-B7 interaction. We therefore evaluated whether the CD28-B7 pathway plays a role in the growth of CTLL-2 cells. Flow cytometry demonstrated that ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.461532.x
更新日期:1996-02-01 00:00:00
abstract::A monolayer depletion/adoptive immunization protocol that biased the immune response towards recognition of placental macrophage (pMO) antigens was established. BALB/c spleen cells immune to human pMO were adsorbed onto monolayers of the B-cell line QIMR-WIL. Monolayer-depleted or unfractionated cells were transferred...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Macrophages play important roles in the clearance of dying and dead cells. Typically, and perhaps simplistically, they are viewed as the professional phagocytes of apoptotic cells. Clearance by macrophages of cells undergoing apoptosis is a non-phlogistic phenomenon which is often associated with actively anti-inflamm...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2004.01959.x
更新日期:2004-09-01 00:00:00
abstract::Cellular adhesion molecules play a central role in leucocyte migration through peripheral blood and tissues. A crucial stage in these events in selectin-mediated adhesion involving E-selectin expressed on activated endothelium interacting with a range of carbohydrate ligands expressed by specific subpopulations of leu...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::alpha 1-Acid glycoprotein (AG), a serum component elevated during acute inflammation, has been implicated in the suppression of various immunological responses. Pretreatment of lymphoid cells with AG at a concentration commonly found in patients with acute inflammation results in the inhibition of mitogen induced lymp...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::The effect of long chain and medium chain fatty acid absorption on transport and mitogen-induced blast transformation of lymphocytes in intestinal lymphatics was investigated. Intestinal lymph was collected from mesenteric lymph duct cannulated rats maintained in Bollman's cage. Following the intraduodenal administrat...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Osteopontin (OPN) is a secreted phosphoprotein found in body fluids (e.g. plasma, urine, milk) and in mineralized tissues. Its expression is increased in many transformed cells and in normal cells exposed to various cytokines. When stimulated with the inflammatory mediators lipopolysaccharide and interferon-gamma, mou...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-691.x
更新日期:1996-08-01 00:00:00
abstract::The Fas/Fas-ligand (FasL) system plays an important role in regulation of apoptosis and the immune response, and is exploited by mycobacteria to evade the immune response. This study was performed to investigate the distribution and levels of FasL and Fas in lymph node granulomas and sera of tuberculous lymphadenitis ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.02080.x
更新日期:2005-02-01 00:00:00
abstract::This study reports the expression and functional characterization of the rat analogue of the human complement regulatory molecule CD59. We here describe the expression in chinese hamster ovary (CHO) cells of rat CD59 and a modified rat CD59 in which an N-glycosylation site at Asn-16 has been deleted by point mutation....
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00200.x
更新日期:1997-04-01 00:00:00
abstract::The expression of Fc mu receptors was investigated using spleen cells from (LOU/M/Wsl X CFY) F1 rats bearing the IR202 tumour, an IgM-producing immunocytoma. It was found that the progressively growing solid tumour was accompanied by an extraordinary expansion of host splenocytes with Fc mu receptors. These cells were...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::Neuropeptides can influence immune effector cell function at both systemic and mucosal immune sites. We examined the ability of substance P (SP) to modulate the natural killer (NK) activity of intestinal intraepithelial leucocytes (IEL). Yac-1 killing by IEL but not splenic cells was increased after either 18 hr prein...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::Functional studies on heteroantisera raised against mouse B cells in rabbits suggest a correlation between thymus-independent B cells and LPS-responsive B cells, and differences in either from thymus-dependent B cells. Furthermore it seems possible to follow B-lymphocyte differentiation from antigen-uncommitted precur...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-05-01 00:00:00
abstract::Prostaglandins are some of the main mediators which control parturition, and their production by intrauterine tissues can be up-regulated by pro-inflammatory cytokines. Anti-inflammatory cytokines may oppose these effects, and in this study we have investigated how two such cytokines affected fetal membrane function. ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00942.x
更新日期:2000-01-01 00:00:00
abstract::Thoracic duct lymphocytes (TDL) from (AS2 x AS)F1 rats previously injected with human serum albumin (HSA) were transferred to 900 r irradiated syngeneic recipients which were challenged with various doses of soluble HSA (s-HSA). TDL from partially tolerant rats, which were deficient in high avidity B cells, produced a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-10-01 00:00:00
abstract::Despite the current availability of Salmonella vaccines, typhoid fever remains a significant public health problem in developing countries. A greater understanding of T-cell activation and the development of immunological memory during Salmonella infection should lead to the development of more effective prophylactic ...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2005.02140.x
更新日期:2005-04-01 00:00:00
abstract::Isolated human mononuclear cells were fractionated according to their membrane characteristics or physical properties. Adherent cells were depleted by filtration through glass columns; phagocytic cells were removed by iron treatment and cell subpopulations capable of forming rosettes with sheep erythrocytes (E), eryth...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::Bursal and splenic lymphocytes in developing chick embryos were examined for the number of antigen binding cells (ABC) which could form rosettes with red blood cells from sheep, guinea-pig, rabbit or dinitrophenylated sheep red blood cells (DNP-SRBC). The kinetics of the development of bursal ABC to rabbit red blood c...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::T lymphocytes are a critical component of the adaptive immune system mediating protection against infection and malignancy, but also implicated in many immune pathologies. Upon recognition of specific antigens T cells clonally expand, traffic to inflamed sites and acquire effector functions, such as the capacity to ki...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12655
更新日期:2017-01-01 00:00:00
abstract::Colorectal cancer is considered a non-immunogenic malignany. One strategy to augment the immunogenicity of such tumours is represented by the expression of costimulatory molecules by gene transfer. Using transfected variants of the human colorectal cancer cell line SW480 we tested various costimulatory molecules (CD80...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00450.x
更新日期:1998-03-01 00:00:00
abstract::It has been known for 15 years that the chicken epidermis contains ATPase+ and major histocompatibility complex class II-positive (MHCII+) dendritic cells. These cells were designated as Langerhans cells but neither their detailed phenotype nor their function was further investigated. In the present paper we demonstra...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02432.x
更新日期:2006-10-01 00:00:00
abstract::The discovery of host-encoded gene products that sense molecular patterns in infectious microbes, and the demonstration of their role in triggering innate and adaptive immune responses, has been a key milestone in our understanding of immunology. Twenty-three years after Janeway first outlined the fundamental concepts...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2012.03587.x
更新日期:2012-08-01 00:00:00
abstract::It has been suggested that tumour cell lysis by gamma-radiation induces a tumoral antigen release eliciting an immune response. It is not clear how a specific immune response in cervical cancer patients is developed after radiotherapy. This study is an attempt to investigate the role of the human papillomavirus type 1...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02912.x
更新日期:2009-04-01 00:00:00