Abstract:
:Trypanosoma cruzi, the causative agent of Chagas' disease, suppresses immune responses during the acute phase and has been shown to induce multiple cellular alterations in activated human T lymphocytes. However, no information is available regarding the effects of this parasite on human B cells. Using an in vitro culture system, in which purified T. cruzi are co-cultured with either peripheral blood mononuclear cells (PBMC) or B-cell-enriched preparations (BCE), we studied whether the organism can induce alterations in DNA synthesis after stimulation with Pansorbin (PS). This response was markedly reduced by the parasite at both suboptimal and optimal PS concentrations, and the extent of the inhibition was augmented as the parasite concentration was increased. Maximal reduction in DNA synthesis was observed when the trypanosomes were incorporated into the cultures at 0 time (i.e. together with PS); the effect was of a much lesser magnitude and undetectable when the parasites were added at 24 and 48 hr, respectively. These results imply that T. cruzi affects a relatively early event during B-cell stimulation. This inference was confirmed by the finding that the proportion of PS-stimulated B cells expressing interleukin-2 (IL-2) receptors was significantly reduced when the parasite was present in the culture. Addition of recombinant human IL-2 did not restore B-cell responsiveness to normal levels. Suppressed B-cell responses were also observed when T. cruzi was separated from the PBMC or the BCE by a cell-impermeable filter, indicating that a soluble factor(s) released by the organism mediated the effect. Accordingly, supernatants of T. cruzi suspensions were found to be suppressive. These results demonstrate for the first time that T. cruzi can affect human B-cell responses and that the mechanism involves inhibition of IL-2 receptor expression.
journal_name
Immunologyjournal_title
Immunologyauthors
Kierszenbaum F,Moretti E,Sztein MBsubject
Has Abstractpub_date
1991-10-01 00:00:00pages
317-22issue
2eissn
0019-2805issn
1365-2567journal_volume
74pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Homogenates from human lung contained a preformed slow reacting substance (pSRS). The pattern of contraction on the guinea-pig ileum by pSRS was indistinguishable from that of SRS-A. The activity of pSRS could not be attributed to the presence of K+, Na+, Ca2+ and Mg2+ ions, or any prostaglandin including PGF2 or its ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::To evaluate the role of the high-affinity monocyte receptor for lipopolysaccharide (LPS), CD14, in the process of tolerance to LPS, the human monocytic cell line Mono-Mac-6 was cultured in the absence or presence of different amounts of LPS. The kinetics of CD14 modulation in these cells showed an initial 4-day period...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::In our efforts to identify products that might be used for active immunotherapy in human immunodeficiency virus (HIV) infection, we have studied synthetic peptides derived from the CD4 attachment site of gp120. Two peptides have emerged with particularly interesting properties. The first (B138) is linear and spans the...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::BALB/c mice develop myasthenic symptoms after immunization with rodent acetylcholine receptor (AChR). After immunization with Torpedo AChR (TAChR), their CD4+ cells become strongly sensitized against a conserved region of the TAChR alpha subunit sequence (residues alpha 304-322), and cross-react vigorously with the ho...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Flow cytometric analyses of the surface immunoglobulins of murine memory B cells revealed the existence of populations expressing multiple isotypes, including an IgM+/IgG+ population that could be stimulated in vitro with antigen to secrete both IgM and IgG. Female BALB/c mice were immunized with R-phycoerythrin (RPE)...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Airway mucus hyperproduction is a common feature of chronic airway diseases such as severe asthma, chronic obstructive pulmonary disease and cystic fibrosis, which are closely associated with neutrophilic airway inflammation. S100A8, S100A9 and S100A12 are highly abundant proteins released by neutrophils and have been...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12352
更新日期:2015-01-01 00:00:00
abstract::The Ager assay was adapted to a porcine lymphocyte-rat high endothelial cell (HEC) system. Using this in vitro assay, the role of porcine CD44 in lymphocyte binding to HEC was examined. The results show that the presence of soluble CD44 molecules did not inhibit the binding of porcine lymphocytes to the cultured rat H...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::In the chicken spleen, certain aspects of the complex of ellipsoid, peri-ellipsoid lymphocyte sheath (PELS), and ring of macrophages are morphologically and functionally similar to the marginal zone in the mammalian spleen. In this study, the role of this marginal zone-like complex was examined during the humoral immu...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::We have used in situ hybridization (ISH) and immunohistochemistry (IHC) to investigate the kinetics of the expression for Fc epsilon RI mRNA (alpha-, beta- and gamma-chains), the alpha-chain protein product, as well as the phenotype of the mRNA- or protein-positive cells in allergen-induced late-phase skin reactions i...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00418.x
更新日期:1998-02-01 00:00:00
abstract::We studied the hs1,2 transcriptional enhancer identified downstream of the human alpha1 gene of the immunoglobulin H (IgH) locus, for which two different allelic configurations (a and b) were previously reported by Southern blotting. By using a polymerase chain reaction (PCR) method we amplified minisatellites within ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01217.x
更新日期:2001-05-01 00:00:00
abstract::Dendritic cells (DC) are the initiators of immune responses and are present in most tissues in vivo. To generate myeloid DC from monocytes (MoDC) in vitro the necessary cytokines are granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Using degenerated primers delineated from other spec...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02319.x
更新日期:2006-04-01 00:00:00
abstract::The adoptive transfer of immunity by means of spleen and lymph node cells has been analysed in rabbits of defined major histocompatibility (RLA) types and immunoglobulin (Ig) allotypes. Previous results had shown that chimaeras formed by transplanting adult cells into RLA-matched newborn hosts become stable B-lymphocy...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Class switching and plasma cell differentiation occur at a high level within all mucosa-associated lymphoid tissues. The different classes of membrane immunoglobulin heavy chains are associated with the Igα/Igβ heterodimer within the B-cell receptor (BCR). Whether BCR isotypes convey specific signals adapted to the co...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2012.03557.x
更新日期:2012-05-01 00:00:00
abstract::Immunohistological techniques have been used to study the stromal cells of the human placenta in both the chorionic villous mesenchyme and the connective tissue underlying the amnion. Throughout gestation many of these cells express an antigen (3C10) that is found on mononuclear phagocytes but not on dendritic cells o...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::The effect on murine immunoglobulin G (IgG) glycosylation of altering IgG production in vivo was assessed in interleukin (IL)-6 transgenic and CD4 knockout mice. C57BL/6 mice carrying the IL-6 transgene showed increased levels of circulating IgG. This was associated with decreased levels of galactose on the IgG oligos...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00896.x
更新日期:1999-11-01 00:00:00
abstract::The ability of freshly isolated primary human alveolar epithelial cells (type II pneumocytes) to induce leucocyte migration across an endothelial monolayer was investigated. Three-way factorial analysis of variance (ANOVA) demonstrated that resting alveolar endothelial cells (AEC) could produce detectable quantities o...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01172.x
更新日期:2001-02-01 00:00:00
abstract::Mice fed a protein-deficient diet containing 2% ovalbumin for 6 weeks (PD) show a reduced spleen IgA plaque-forming cell (PFC) response after oral immunization with sheep red blood cells (SRBC), compared to the response of mice fed a control diet containing 20% ovalbumin (C). This reduced IgA PFC response was partiall...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::Immunopotentiation has been demonstrated when Klebsiella O3 lipopolysaccharide (KO3 LPS), which possesses a linear mannan as the O-specific side chain, was injected subcutaneously into endotoxin resistant C3H/HeJ mice together with soluble protein antigens. The LPS exhibited significantly positive adjuvant effects on ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::Metabolism of ss-DNA in Swiss Webster mice and NZB/W mice was studied. The delay in intra-hepatic catabolism of ssDNA in old NZB/W female mice seems to correlate with the presence of increased anti-DNA antibodies; similar to effects produced in Swiss Webster mice that have been pretreated with a small dose of carbon b...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::The phospholipid platelet-activating factor 1-0-hexadecyl-2-acetyl-SN-glycero-3-phosphorylcholine (AGEPC) initiates platelet function by interacting specifically with 1399 +/- 498 (mean +/- SD) high-affinity membrane receptors per platelet. In studies designated to characterize the high affinity binding site, AGEPC-hu...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::We investigated the individual CD8+ populations with natural killer (NK) cell markers (NK-type T cell); CD56 single positive (CD56)-T cells, CD56/CD57 double positive (DP)-T cells and CD57 single positive (CD57)-T cells in the peripheral blood. All NK-type T-cell populations expressed CD122 and intermediate levels of ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01575.x
更新日期:2003-02-01 00:00:00
abstract::As the burden of infectious diseases becomes reduced in many countries, a remarkable increase in the incidence of allergies has occurred. The basis for the rise in atopic disorders as a correlate of the decline in infectious diseases has not been defined. In the present study, we tested experimentally whether prior sy...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00856.x
更新日期:1999-11-01 00:00:00
abstract::Guinea-pig erythrocytes that had been exposed to influenza A virus or Vibrio cholerae neuraminidase activated the classical complement pathway in autologous serum. Because all viral particles were eluted from the treated cells, activation was not dependent on anti-viral antibodies or on the particles themselves. After...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract::The relationship between allograft rejection and the response in the one-way mixed leucocyte reaction (MLR) of the WBC of allograft recipients was investigated. The responses of the WBC were assessed prior to skin allografting and subsequent to rejection of the allograft. The optimal MLR response of the WBC from the a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Over the past 10 years we have made great strides in our understanding of T helper cell differentiation, expansion and effector functions. Within the context of T helper type 2 (Th2) cell development, novel innate-like cells with the capacity to secrete large amounts of interleukin-5 (IL-5), IL-13 and IL-9 as well as ...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2011.03497.x
更新日期:2011-12-01 00:00:00
abstract::This study examines the expression of mRNA for the Th2 cytokine, interleukin-4 (IL-4). Peripheral blood mononuclear cells from deer infected with Mycobacterium bovis or vaccinated with live or killed M. bovis bacillus Calmette-Guérin (BCG) were cultured with mycobacterial antigens. IL-4 mRNA production was assayed usi...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.1996.tb00014.x
更新日期:1996-06-01 00:00:00
abstract::Docosahexaenoic (22:6 n-3) and eicosapentaenoic acid (20:5 n-3) stimulated the oxygen-dependent respiratory burst in intact neutrophils in a dose-dependent manner as measured by either superoxide dismutase (SOD)-inhibitable cytochrome c reduction and lucigenin-dependent chemiluminescence. A number of longer chain hexa...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Heat or chemically aggregated IgA or IgG stimulated degranulation of neutrophils with comparable efficiency. The same aggregates induced a neutrophil respiratory burst which could be measured by lucigenin-enhanced chemiluminescence. Serum IgA1 or IgA2 coated onto microtitre plates were both capable of inducing a respi...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::Currently, the limited supply and stability of some human autoantigens pose formidable difficulties in characterizing patients' T cells specific for them; recombinant preparations may contain bacterial contaminants, and synthetic peptides have arbitrarily chosen start and stop points. In order to provide a stable anti...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::The current study sought to delineate the gene expression profile of the host response in the caecum and colon during acute infection with Clostridium difficile in a mouse model of infection, and to investigate the nature of the unfolded protein response in this process. The infected mice displayed a significant up-re...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12122
更新日期:2013-09-01 00:00:00