Abstract：
:Airway mucus hyperproduction is a common feature of chronic airway diseases such as severe asthma, chronic obstructive pulmonary disease and cystic fibrosis, which are closely associated with neutrophilic airway inflammation. S100A8, S100A9 and S100A12 are highly abundant proteins released by neutrophils and have been identified as important biomarkers in many inflammatory diseases. Herein, we report a new role for S100A8, S100A9 and S100A12 for producing MUC5AC, a major mucin protein in the respiratory tract. All three S100 proteins induced MUC5AC mRNA and the protein in normal human bronchial epithelial cells as well as NCI-H292 lung carcinoma cells in a dose-dependent manner. A Toll-like receptor 4 (TLR4) inhibitor almost completely abolished MUC5AC expression by all three S100 proteins, while neutralization of the receptor for advanced glycation end-products (RAGE) inhibited only S100A12-mediated production of MUC5AC. The S100 protein-mediated production of MUC5AC was inhibited by the pharmacological agents that block prominent signalling molecules for MUC5AC expression, such as mitogen-activated protein kinases, nuclear factor-κB (NF-κB) and epidermal growth factor receptor. S100A8, S100A9 and S100A12 equally elicited both phosphorylation of extracellular signal-regulated kinase (ERK) and nuclear translocation of NF-κB/degradation of cytosolic IκB with similar kinetics through TLR4. In contrast, S100A12 preferentially activated the ERK pathway rather than the NF-κB pathway through RAGE. Collectively, these data reveal the capacity of these three S100 proteins to induce MUC5AC production in airway epithelial cells, suggesting that they all serve as key mediators linking neutrophil-dominant airway inflammation to mucin hyperproduction.
journal_name
Immunologyjournal_title
Immunologyauthors
Kang JH,Hwang SM,Chung IYdoi
10.1111/imm.12352subject
Has Abstractpub_date
2015-01-01 00:00:00pages
79-90issue
1eissn
0019-2805issn
1365-2567journal_volume
144pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:Neuropeptides can influence immune effector cell function at both systemic and mucosal immune sites. We examined the ability of substance P (SP) to modulate the natural killer (NK) activity of intestinal intraepithelial leucocytes (IEL). Yac-1 killing by IEL but not splenic cells was increased after either 18 hr prein...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Expression of Fc gamma receptors on human placental trophoblast was investigated by immunostaining and immunoblotting using a panel of Fc gamma receptor monoclonal antibodies (mAb). Fc gamma receptors typical of other cell types were not detected on syncytiotrophoblast in term placentae when transplacental IgG transpo...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:The linker protein LAT is expressed mainly in T and natural killer (NK) cells. LAT-deficient mice have an arrest of intrathymic T-cell development at the CD4+ CD8+ stage and lack mature T cells in the periphery. However, no gross abnormality in development and function of the B and NK cells has been described. Here we...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2003.01671.x
更新日期：2003-07-01 00:00:00
abstract：:Virus plaque assay (VPA) was utilized for the quantitative evaluation of activated lymphocytes. We examined what types of cells, especially which of activated T and non-T lymphocytes, were detected as infective centres after infection with vesicular stomatitis virus. Marked increases in DNA synthesis and in virus-plaq...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:The migration of immune cells through the extracellular matrix (ECM) towards inflammatory sites is co-ordinated by receptors recognizing ECM glycoproteins, chemokines and proinflammatory cytokines. In this context, galectins are secreted to the extracellular milieu, where they recognize poly-N-acetyllactosamine chains...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00746.x
更新日期：1999-05-01 00:00:00
abstract：:Goats were immunized with membrane fractions of the L5178Y tumour syngenic to DBA/2 mice. IgG fractions of this antiserum were made tumour specific by repeated in vitro and in vivo absorptions with normal cells and concentrated by adsorption to and elution from tumour cells. In vivo studies indicated that the accumula...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:CD4 T cells help immune responses, but knowledge of how memory CD4 T cells are regulated and how they regulate adaptive immune responses and induce immunopathology is limited. Using adoptive transfer of virus-specific CD4 T cells, we show that naive CD4 T cells undergo substantial expansion following infection, but ca...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12771
更新日期：2017-10-01 00:00:00
abstract：:A common factor existing among autoimmune target antigens was sought in association with their developmental expression during organogenesis. Autoimmunity against a certain organ was experimentally induced in rats by deliberate immunization with whole tissue extract of the respective organ. Histopathological changes i...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:It is shown that the binding of bivalent anti-immunoglobulin preparations to the surface immunoglobulin of murine B lymphocytes as well as the binding of aggregates of IgG or immune complexes does not lead to detectable increase in cell division in microcultures. Treating lymphocytes with immune complexes and aggregat...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：:While many aspects of memory T-cell immunobiology have been characterized, we suggest that we know only a fraction of the effector functions that CD4 T cells can bring to bear during secondary challenges. Exploring the full impact of memory CD4 T-cell responses is key to the development of improved vaccines against ma...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2010.03259.x
更新日期：2010-05-01 00:00:00
abstract：:An autoreactive T-cell clone, MTg9B3, specific for mouse thyroglobulin, has been characterized. This clone has properties consistent with it an being autoreactive T helper cell. It is I-A restricted, with a surface phenotype of Thy 1+, L3T4+, Ly2-, and produces a number of non-specific lymphokine activities following ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:The effect of intravenous (i.v.) administration of bacterial alpha-amylase (B alpha A) on the IgG antibody response to a subsequent challenge with B alpha A in incomplete Freund's adjuvant (IFA) varied with the difference in responsiveness of the parental strains. High-responder C3H/He (C3) mice given injections of ei...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:We report that interleukin (IL)-4 and IL-10 can significantly up- or down-regulate CXC chemokine receptor 4 (CXCR4) expression on CD4+ T lymphocytes, respectively. Stromal cell-derived factor-1alpha (SDF-1alpha)-induced CD4+ T-lymphocyte chemotaxis was also correspondingly regulated by IL-4 and IL-10. IL-4 and IL-10 u...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00954.x
更新日期：2000-03-01 00:00:00
abstract：:Cocultures of dispersed human fetal liver cells with murine Swiss 3T3 fibroblasts resulted in the development of human mast cells after 1 to 4 weeks of culture. Mast cells were detected by immunohistochemistry using a murine monoclonal anti-tryptase antibody, before metachromasia appeared with toluidine blue. When sub...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Purified pertussis toxin (PPT) is a potent mitogen for human T lymphocytes and is shown to cause rapid calcium mobilization in resting T cells, a T-cell line and CD3- lymphocytes with natural killer (NK) activity. In resting T cells the PPT activation is associated with cytoplasmic alkalinization. A similar rise in in...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:We have established lines and clones of helper T cells specific for the antigen fowl gamma globulin (FGG) and maintained these cells in continuous proliferation in vitro for up to 8 months. During this period the specific helper activity was assessed in vitro in T cell-dependent anti-hapten responses. These pure popul...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:Activation of human eosinophils by platelet-activating factor (PAF) involves multiple signal transduction pathways. Among these, protein kinase C has been demonstrated both to mediate respiratory burst and to suppress an alternative pathway of activation of respiratory burst and arachidonic acid metabolism in eosinoph...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00037.x
更新日期：2000-06-01 00:00:00
abstract：:When Trypanosoma cruzi is co-cultured with activated human lymphocytes the latter manifest a marked level of suppression in their capacity to express high-affinity interleukin-2 receptors (IL-2R). In this study we asked whether this effect could result from reduced expression of the p75 component of the IL-2R, a condi...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Much of the pathology of malaria may be due to the interactions of cytokines, especially tumour necrosis factor (TNF), with various cell types, including endothelial cells, with consequent widespread systemic effects. It has been shown previously that heat-stable exoantigens in the supernatants of blood-stage parasite...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
abstract：:Several cloned, non-leukaemic mouse haemopoietic cell lines are believed to be uniquely dependent on interleukin-3 (IL-3) for their survival. All of these lines require the addition of WEHI-3B cell-conditioned medium for their growth. We have examined the growth requirements of four IL-3-dependent cell lines and found...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:The generation of effective immunity requires that antigen-specific T cells are activated, clonally expanded and ultimately eliminated by apoptosis. The involvement of CD95-mediated apoptosis in T-cell elimination is well established, but the conditions which regulate the death pathway under normal circumstances are s...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00925.x
更新日期：1999-12-01 00:00:00
abstract：:A murine anti-B monoclonal antibody was obtained by the hybridoma technique. This antibody called anti-B (b-183) is of IgM nature; it is capable of agglutinating normal B, B3, Bx, cis AB and some acquired B red cells. Its association constant is 1.1 X 10(8) l/mol, and appears high compared to those of the monoclonal a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Detection of immune complexes by inhibition of antibody-dependent cellular cytotoxicity (ADCC) is based on the principle that soluble complexes can compete with target cell-bound antibody for receptors (FcR) on cytotoxic lymphocytes. The objective of this study was to define a cytotoxicity system for the determination...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:Newborn mammals are highly susceptible to respiratory infections. Although maternal antibodies (MatAb) offer them some protection, they may also interfere with their systemic immune response to vaccination. However, the impact of MatAb on the neonatal mucosal immune response remains incompletely described. This study ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12222
更新日期：2014-04-01 00:00:00
abstract：:Following immunization with various lambda Bence-Jones proteins, over 100 hybridomas were established. A majority of the monoclonal antibodies (McAb) produced were demonstrated to recognize epitopes common to all lambda chains or unique to the immunogen. Twenty-nine McAb exhibited profiles of 'restricted' reactivity. ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：:Specific pathogen-free B6D2 mice were infected intravenously with 10(8) viable BCG, M. habana or M. simiae and the level of tuberculin hypersensitivity to 2.5 micrograms PPD or cytoplasmic protein antigens (CPA) prepared from the other organisms was determined using the footpad swelling test with increasing time after...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:The role of Foxp3(+) regulatory T (Treg) cells in the course of the early hyper-inflammatory and subsequent hypo-inflammatory phases of sepsis is ambiguous. Whereas Nrp1 expression has been reported to discriminate natural Treg cells from induced Treg cells, the Treg cell stability depends on the methylation status of...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12490
更新日期：2015-09-01 00:00:00
abstract：:The effects of anti-Ia antibodies and cyclosporin A (CsA) on phenotypic differentiation of murine thymocytes were assessed in organ-cultured fetal thymuses. Both agents specifically abrogated the generation of CD4+8- thymocytes. Immunohistochemical studies revealed that Ia antigen on the organ-cultured thymic epitheli...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Previous studies in our laboratory have shown that bone-marrow-derived mast cells (BMMC) could present immunogenic peptides, from soluble antigens endocytosed through fluid phase, only if they were subjected to a 48-hr treatment with interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF). I...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00789.x
更新日期：1999-06-01 00:00:00
abstract：:Preincubation of macrophages with immune complexes suppresses the interferon-gamma (IFN-gamma) induction of tumouricidal activity and Ia surface expression. The studies reported in this manuscript were designed to test if immune complexes alter the interaction of IFN-gamma with macrophages. The binding of IFN-gamma to...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
