Abstract：
:Following immunization with various lambda Bence-Jones proteins, over 100 hybridomas were established. A majority of the monoclonal antibodies (McAb) produced were demonstrated to recognize epitopes common to all lambda chains or unique to the immunogen. Twenty-nine McAb exhibited profiles of 'restricted' reactivity. Analysis with a panel of serologically defined antigens allowed McAb recognizing the lambda II subgroup to be identified. Other McAb recognized epitopes selectively expressed by lambda II and lambda III subgroup proteins. A further twenty-one McAb exhibited individual specificity profiles, some of which detect minor lambda chain subpopulations. Several of these McAb have potential for analysis of the clonality of specific antibody responses and for detecting monoclonal B-cell proliferations.
journal_name
Immunologyjournal_title
Immunologyauthors
Walker MR,Solomon A,Ling NR,Brown B,Lowe JA,Hardie D,Jefferis Rsubject
Has Abstractpub_date
1986-11-01 00:00:00pages
467-71issue
3eissn
0019-2805issn
1365-2567journal_volume
59pub_type
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