Abstract:
:We describe a high-throughput screening system to detect interactions between leucocyte surface proteins, taking into account that these interactions are usually of very low affinity. The method involves producing the extracellular regions of leucocyte proteins with tags so that they can be bound to nanoparticles to provide an avid reagent to screen over an array of 36 similar proteins immobilized using the Proteon XPR36 with detection by surface plasmon resonance. The system was tested using established interactions that could be detected without spurious binding. The ability to detect new interactions was shown by identifying a new interaction between carcinoembryonic antigen-related cell adhesion molecule 1 and carcinoembryonic antigen-related cell adhesion molecule 8.
journal_name
Immunologyjournal_title
Immunologyauthors
Jiang L,Barclay ANdoi
10.1111/j.1365-2567.2009.03153.xsubject
Has Abstractpub_date
2010-01-01 00:00:00pages
55-61issue
1eissn
0019-2805issn
1365-2567journal_volume
129pub_type
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