Abstract:
:Individual young adult, middle-aged, and old C57BL/6J male mice were tested for in vitro generated proliferative and cytotoxic responses to H-2 alloantigens under a variety of sensitization conditions. Proliferation in mixed lymphocyte culture (MLC) had decreased by 14 months of age (middle-aged), whether measured by directly assaying cultures in microtitre plates (micro MLC) or by labelling aliquots taken from large culture tubes (macro MLC). Cytotoxicity did not decline until a later age if sensitization was done in large tubes (macro cell-mediated lympholysis, CML). When cytotoxic activity was assayed by measuring lysis after addition of chromated cells to MLCs in microtitre plates (micro CML), differences were revealed between young and middle-aged animals. However, these conditions were suboptimal for generation of cytotoxicity even in young controls and showed even lower responses in the middle-aged group. It was concluded that proliferation showed an earlier, more severe decline than cytotoxicity with age as the proliferative response had declined by middle-age under all sensitization conditions used. With optimal sensitization conditions, senescent mice (26--30 months) showed a four- to ten-fold decrease in cytotoxicity compared with young adult mice.
journal_name
Immunologyjournal_title
Immunologyauthors
Gottesman SR,Kristie JA,Walford RLsubject
Has Abstractpub_date
1981-11-01 00:00:00pages
607-16issue
3eissn
0019-2805issn
1365-2567journal_volume
44pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::To evaluate the T-cell large-scale differential gene expression in systemic lupus erythematosus (SLE) patients presenting with glomerulonephritis we studied SLE patients before and after immunosuppressive treatment. Large-scale gene expression of peripheral blood mononuclear T cells was evaluated using cDNA microarray...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01929.x
更新日期:2004-09-01 00:00:00
abstract::The investigation of the role of the macrophage in cutaneous leishmaniasis has been prompted by observations of the clinical behaviour of the infection. In contrast to the self-healing oriental sore, chronic leishmaniasis is characterized by persistent lesions and leishmania recidiva by lesions that flare up locally l...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-10-01 00:00:00
abstract::Specific pathogen-free B6D2 mice were infected intravenously with 10(8) viable BCG, M. habana or M. simiae and the level of tuberculin hypersensitivity to 2.5 micrograms PPD or cytoplasmic protein antigens (CPA) prepared from the other organisms was determined using the footpad swelling test with increasing time after...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
abstract::Primary contact sensitivity (CS) to Ox and non-specific unresponsiveness to other unrelated delayed-type hypersensitivity (DTH) containing CS were induced simultaneously by i.v. administration of oxazolone(Ox)-conjugated spleen cells. The subpopulation of spleen cells which serves as carriers for the induction of prim...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::Veiled cells (VC), isolated from the thoracic duct of irradiated lymphadenectomized mice (MLNX) or peritoneal macrophages (PM phi were incubated with isotope-labelled hen egg lysozyme (HEL), purified protein derivative (PPD) or keyhole limpet haemocyanin (KLH) in vitro. About 2-10 times less antigen was associated wit...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Studies in long-term non-progressors (LTNP) have suggested that the quality of the CD8(+) response may involve protective human leucocyte antigen (HLA) class I alleles. However, studies examining the expansion ability of different functional CD8(+) T cells and their association with HLA class I alleles are lacking. LT...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03490.x
更新日期:2011-11-01 00:00:00
abstract::In the study described here, the effect of human tears and purified lactoferrin was investigated on the deposition of complement components on solid-phase bound immune complexes. After incubating immune complexes with fresh normal human serum, the deposition of complement components (C3, C4 and C5) was measured with a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::Mice injected with rat erythrocytes developed anti-erythrocyte autoantibodies which reached a plateau at 4-12 weeks, then gradually declined until at about 24 weeks the majority of mice were negative. In such recovered mice re-challenge with rat erythrocytes produced an accelerated peak of autoantibody and a much more...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::The influence of the thymus and the possible requirement for surface immunoglobulin expression for the development of K and NK activity were assessed in rats. This species was chosen in preference to mice as they show good levels of K-cell activity. Studies were carried out in athymic (rnu/rnu) rats some of which were...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::The negative role exerted by cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) in the regulation of T-cell activity, as induced by T-cell receptor (TCR)/CD3 and CD28 costimulation, has been widely described. In the present work we investigated the role of CTLA-4 in the control of cell activation, as induced by cost...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01493.x
更新日期:2002-10-01 00:00:00
abstract::Vitamin A and its active metabolite retinoic acid are essential for the development and function of many tissues including the immune system. The induction of mucosal homing receptors on T and B cells by mucosal dendritic cells (DC) depends on the presence of vitamin A. Recent studies indicate that also the differenti...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12228
更新日期:2014-05-01 00:00:00
abstract::The output and immunoglobulin class of antibody-containing cells (ACC) in lymph from different regions of adult sheep has been compared following intraperitoneal or subcutaneous injection of ovalbumin in Freund's complete adjuvant (FCA). Intraperitoneal injection of antigen in FCA elicited no response at all in poplit...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-04-01 00:00:00
abstract::Despite the current availability of Salmonella vaccines, typhoid fever remains a significant public health problem in developing countries. A greater understanding of T-cell activation and the development of immunological memory during Salmonella infection should lead to the development of more effective prophylactic ...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2005.02140.x
更新日期:2005-04-01 00:00:00
abstract::We have used in situ hybridization (ISH) and immunohistochemistry (IHC) to investigate the kinetics of the expression for Fc epsilon RI mRNA (alpha-, beta- and gamma-chains), the alpha-chain protein product, as well as the phenotype of the mRNA- or protein-positive cells in allergen-induced late-phase skin reactions i...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00418.x
更新日期:1998-02-01 00:00:00
abstract::Tick larvae (Boophilus microplus) were extracted, fractionated by gel filtration and ion-exchange chromatography, and the fractions assayed by enzyme-linked immunosorbent assay (ELISA) against sera from cattle immunized and protected by antigens from the mid-gut of adult ticks. Unfractionated, whole larval extracts an...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract::Various strains of the murine mycoplasma M. neurolyticum have been shown to induce extensive blast transformation of mouse lymphocytes, comparable in strength to mitogenicity exerted by these mycoplasma species on rat lymphocytes. The data summarized in this report demonstrate that this mitogenic effect is non-specifi...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-04-01 00:00:00
abstract::The suppression of immune responsiveness to heterologous antigenic stimulation during concurrent infection with Nematospiroides dubius was reproduced using soluble antigens derived from adult parasites. Immunosuppression appeared to be selective in that the administration of equivalent quantities of an irrelevant hete...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::All but one of nine Acholeplasma strains were susceptible to killing by gnotobiotic-calf serum while all but one of fifteen Mycoplasma strains were resistant. The mechanism of killing was examined using inhibitors of complement, viz. EDTA. MgEGTA. epsilon-amino caproic acid, and also by desalting serum and adding MgCl...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::Interleukin-12 (IL-12) is important in the regulation of resistance to Toxoplasma gondii in mice with severe combined immunodeficiency (SCID). The protective ability of IL-12 in SCID mice appears to be through its activity on natural killer (NK) cells to induce production of interferon-gamma (IFN-gamma). In this study...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Immunohistological techniques have been used to study the stromal cells of the human placenta in both the chorionic villous mesenchyme and the connective tissue underlying the amnion. Throughout gestation many of these cells express an antigen (3C10) that is found on mononuclear phagocytes but not on dendritic cells o...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::Activation of lymph nodes by the T-cell mitogens PHA and Con A is correlated with a depletion of lymphatic mast cells. This result, and our earlier reports on the depletion of mast cells in lymph nodes stimulated by allogeneic and tumour cells, as well as on the elevation of mast cell number in thymus-less 'nude' mice...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::Thymocytes incubated with either ATP or GTP exhibit a brief period of enhanced DNA synthesis in contrast to the prolonged period of enhanced synthesis observed when thymocytes were incubated with concanavalin A. The cells responding to nucleotides represent a sub-population of theta antigen-bearing T cells comprising ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract::In order to analyse the molecular mechanism of lymphocyte activation defect in MRL/MP-lpr (MRL/1) mice, c-myc proto-oncogene expression was examined in MRL/1 lymph node cells stimulated by various agents. Since a transient increase of c-myc RNA in early hours is required for lymphocyte activation, detection of c-myc m...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::The resistance and susceptibility of T cells to human immunodeficiency virus (HIV)-gp120 induced anergy was examined. Antigen-dependent proliferation of polyclonal T cells was markedly inhibited by gp120, whereas from the analysis of monoclonal populations, T cells resistant to the effects of gp120 could be identified...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Trinitrophenyl (TNP)-specific humoral response was tested after immunization with TNP-protein conjugates composed of either native proteins or proteins modified by chlorination. Significant enhancement of the hapten-specific response was observed as a result of carrier chlorination. This effect was dose dependent and ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract::Sodium penicillin was conjugated to sheep erythrocytes and optimal quantities, added to a 5% SRBC suspension, were determined for haemagglutination (12-5 mg/ml) and for haemolysis (50 mg/ml) using carp antibodies and carp complement. The epitope density on the BSA molecule was gradually increased, when increasing amou...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::The role of the mouse homocytotropic antibodies in passive cutaneous anaphylaxis reaction was investigated. One class of antibody was heat stable, detected at 2 h but not at 48 h after passive transfer, and belonged to a sublcass of mouse IgG. The other was heat labile, detected at 2 h and 48 h after passive transfer,...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-04-01 00:00:00
abstract::In this study we followed the in vivo fate and distribution of hapten-coupled sheep red blood cells (TNP-SRBC) coated with 125I-labelled anti-hapten antibody in mice. The majority of these complexes was rapidly taken up by the liver, and by macrophages in the marginal zone of the spleen. Within a few hours, however, m...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Trypanosoma cruzi, the causative agent of Chagas' disease, is a protozoan parasite that infects humans and other mammals in Central and Latin America. Several alterations of the immune response after infection have been described, such as severe immunosuppression of both cellular and humoral responses and massive poly...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00498.x
更新日期:1998-06-01 00:00:00
abstract::Toll-like receptors (TLR) are sentinel receptors capable of recognizing pathogen-associated molecule patterns (PAMP) such as lipopolysaccharide (LPS) and CpG-containing oligonucleotides (CpG ODN). TLR2 and TLR4 are major receptors for Gram-positive and Gram-negative bacterial cell wall components, respectively. TLR9 i...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01401.x
更新日期:2002-05-01 00:00:00