Abstract:
:The anti-C6 monoclonal antibody WU 6-4 was shown to be unequivocally native restricted since it neither binds to the terminal complement complex (TCC) nor to C5b6. In addition, it was shown to inhibit TCC formation by interfering with C5b6 generation. Using the pUEX expression system and C6 cDNA the WU 6-4 epitope was mapped to the third thrombospondin repeat of C6. This protein domain may therefore contribute to the C5 binding site of C6 and be involved in terminal complement complex assembly. The presence of the epitope in rabbit C6 indicates a useful model for studying inhibition of TCC formation in vivo.
journal_name
Immunologyjournal_title
Immunologyauthors
Würzner R,Mewar D,Fernie BA,Hobart MJ,Lachmann PJsubject
Has Abstractpub_date
1995-06-01 00:00:00pages
214-9issue
2eissn
0019-2805issn
1365-2567journal_volume
85pub_type
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