Abstract:
:The role of Foxp3(+) regulatory T (Treg) cells in the course of the early hyper-inflammatory and subsequent hypo-inflammatory phases of sepsis is ambiguous. Whereas Nrp1 expression has been reported to discriminate natural Treg cells from induced Treg cells, the Treg cell stability depends on the methylation status of foxp3-TSDR. To specifically evaluate the role of Foxp3(+) Treg cells in the early and late phases of sepsis, we induced sepsis by caecal ligation and puncture and subsequent Pseudomonas aeruginosa lung infection in a DEREG (DEpletion of REGulatory T cells) mouse model. We found an increase of Foxp3(+) Treg cells to all CD4(+) T cells during murine sepsis. Using a new methylation-sensitive quantitative RT-PCR method and deep amplicon sequencing, we demonstrated that natural (Nrp1(+) Foxp3(+) ) Treg cells and most induced (Nrp1(-) Foxp3(+) ) Treg cells are stable and exhibit unmethylated foxp3-TSDR, and that both Treg populations are functionally suppressive in healthy and septic mice. DEREG mice depleted of Foxp3(+) Treg cells exhibit higher disease scores, mortality rates and interleukin-6 expression levels than do non-depleted DEREG mice in early-phase sepsis, a finding indicating that Foxp3(+) Treg cells limit the hyper-inflammatory response and accelerate recovery. Treg cell depletion before secondary infection with P. aeruginosa 1 week after caecal ligation and puncture does not influence cytokine levels or the course of secondary infection. However, a moderate Treg cell recurrence, which we observed in DEREG mice during secondary infection, may interfere with these results. In summary, Treg cells contribute to a positive outcome after early-phase sepsis, but the data do not support a significant role of Treg cells in immune paralysis during late-phase sepsis.
journal_name
Immunologyjournal_title
Immunologyauthors
Tatura R,Zeschnigk M,Hansen W,Steinmann J,Vidigal PG,Hutzler M,Pastille E,Westendorf AM,Buer J,Kehrmann Jdoi
10.1111/imm.12490subject
Has Abstractpub_date
2015-09-01 00:00:00pages
144-56issue
1eissn
0019-2805issn
1365-2567journal_volume
146pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Factor B-like activity associated with human peripheral blood lymphocytes was first described by Halbwachs & Lachmann (1976), who employed a functional assay. In this present study, clones of B cells from patients with chronic lymphatic leukaemia (CLL) and lymphoblastoid 'Raji' cells were used. The surface of these ce...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::Membrane attack complexes of complement occur around thyroid follicles in Graves' disease and Hashimoto's thyroiditis. The lytic potential of such complexes is controlled by membrane-bound and fluid phase regulators and we have investigated the role of these in autoimmune thyroid disease. By immunohistochemical staini...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Recently it has been shown that selective subconjunctival macrophage depletion reduced the incidence and severity of stromal herpes simplex virus (HSV) keratitis in mice. In this study, we examined the effect of conjunctival macrophage depletion on the corneal and systemic T-cell-mediated immune response. BALB/c mice ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01477.x
更新日期:2002-09-01 00:00:00
abstract::Using KOLT-2 monoclonal antibody (mAb) recognizing a 44,000 molecular weight (MW) T-cell activation antigen (CD28), we observed the co-expression of KOLT-2 (CD28) antigen and Tac (CD25) antigen, associated with a 55,000 MW chain of the interleukin-2 receptor (IL-2R) complex. IL-2R (p55), on several T-cell lines transf...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::Spleen cells from LAF1 mice primed with photo-oxidized antigen B exhibit helper T-cell activity when assayed in an adoptive transfer system with spleen cells from mice primed with timothy pollen extract and treated with anti-thy 1.2 serum and complement. These helper cells, when placed in culture with antigen B, secre...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Infection of susceptible mice with murine cytomegalovirus (MCMV) induces persistent inflammation, and the production of autoantibodies reactive with large numbers of proteins from all major organs. However the roles of polyclonal B-cell activation, autoreactive T-helper cells and host-virus cross-reactions in these ph...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Monoclonal antibodies were produced following immunization of mice with live infective larvae of Brugia malayi. One of these, 46.08.76, is an antibody that promotes adherence of mouse peritoneal macrophages and human peripheral blood leucocytes to the infective larvae of B. malayi and Wuchereria bancrofti, respectivel...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::The kinetics of ingestion and degradation of guinea-pig IgG2-containing soluble immune complexes by guinea-pig macrophages and neutrophils have been compared and aspects of the biochemical control of these events in neutrophils have been investigated using a selection of inhibitors. Differences were observed between t...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract::The role of gut-derived CD4+ T cells in clearance of Pseudomonas aeruginosa from the lung was studied by cell transfer experiments. Mesenteric lymph node cells from unimmunized rats, or rats orally immunized with either killed P. aeruginosa or Helicobacter pylori, were transferred to naive rats which were subsequently...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::Human β-defensin 3 (hBD-3) activates antigen-presenting cells through Toll-like receptors (TLRs) 1/2. Several TLR1/2 agonists have been identified but little is known about how they might differentially affect cellular activation. We compared the effects of hBD-3 with those of another TLR1/2 agonist, Pam(3) CSK(4) , i...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03475.x
更新日期:2011-10-01 00:00:00
abstract::The shedding and resynthesis of Fc, C3 receptors, and receptors binding SRBC has been studied in normal peripheral lymphocytes, in lymphocytes from CLL patients and in a Raji cell line during 4 h incubation at 37 degrees. During the first part of the incubation, an increased release of receptors was observed. The shed...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::In our efforts to identify products that might be used for active immunotherapy in human immunodeficiency virus (HIV) infection, we have studied synthetic peptides derived from the CD4 attachment site of gp120. Two peptides have emerged with particularly interesting properties. The first (B138) is linear and spans the...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::Studies of tuberculosis have suggested a shift in dominance from a T helper type 1 (Th1) towards a Th2 immune response that is associated with suppressed cell-mediated immune (CMI) responses and increased humoral responses as the disease progresses. In this study a natural host disease model was used to investigate th...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.02003.x
更新日期:2005-01-01 00:00:00
abstract::Immunohistological techniques have been used to study the stromal cells of the human placenta in both the chorionic villous mesenchyme and the connective tissue underlying the amnion. Throughout gestation many of these cells express an antigen (3C10) that is found on mononuclear phagocytes but not on dendritic cells o...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::The migration of lymphocytes and neutrophils into skin sites stimulated with chemotactic agonists or with cytokines known to induce leucocyte-endothelial adhesion molecules was examined in sheep. Lymphocytes, collected from efferent prefemoral lymph, labelled in vitro with [111In]oxine and reinjected intravenously, mi...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Trypanosoma cruzi, the causative agent of Chagas' disease, suppresses immune responses during the acute phase and has been shown to induce multiple cellular alterations in activated human T lymphocytes. However, no information is available regarding the effects of this parasite on human B cells. Using an in vitro cult...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::The in vitro treatment with Fusarenon-X, a mycotoxin produced by Fusarium nivale Fn 2B, depressed the mitogenic responses of mouse lymphocytes to the T-cell mitogens, phytohaemagglutinin (PHA) and concanavalin A (Con A), but to a lesser extent to B-cell mitogen, a bacterial lypopolysaccharide (LPS). The in vitro treat...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
abstract::The purpose of this work was to determine if the fine specificity of T cells differed between mice immunized with an antigen in a T helper 1 (Th1) cytokine-dominated environment as compared with a T helper 2 (Th2) cytokine-dominated environment. It was found that splenic T cells from mice immunized with mycobacterial ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00007.x
更新日期:1998-04-01 00:00:00
abstract::Expression of the lymph node homing and CC-chemokine receptor 7 (CCR7), with L-selectin (CD62L), has been shown to divide human memory T cells into two functionally distinct subsets. We generated a polyclonal antibody against murine CCR7 and used this antibody to study CCR7 expression on murine T-cell subsets. Using f...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01727.x
更新日期:2003-10-01 00:00:00
abstract::Membrane sialoglycoprotein (GP) of mouse erythrocytes were incorporated into artificial membranes of liposomes which also contained DNP-aminocaproylphosphatidylethanolamine hapten. The liposomes were tested for their potential to modulate the anti-hapten antibody response in mice as a plausible model of haptenated iso...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
abstract::Guinea-pigs immunized with homogenates of unfed larvae and nymphs of the tick Rhipicephalus appendiculatus developed significant levels of protective immunity to infestation with adults of this species. The mean engorged weight of female ticks feeding on immunized animals (181.96 +/- 05.63 mg and 170.11 +/- 11.54 mg) ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::The migration of leucocytes into tissues is a process mediated by leucocyte endothelial interactions, in which adhesion receptors play a crucial role. Recently, it was found that 80-90% of T cells in inflammatory skin diseases were reactive to the monoclonal antibody (mAb) HECA-452+ in contrast to inflamed non-cutaneo...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::The immunological basis for the production of autoantibodies characteristic of systemic lupus erythematosus (SLE) against a wide range of antigens remains obscure. The specificity of (NZB x NZW)F1 (BWF1) or MRL/Mp-lpr/lpr (MRL/lpr) mouse monoclonal antibodies (mAb) was examined by immunofluorescence, immunoblotting an...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::The influence of changes of the cation-transporting activity of the membrane Na,K ATP-ase of human peripheral blood lymphocytes on their ability to form 'early' and 'late' rosettes with SRBC was investigated. It was shown that inhibition of the Na,K ATP-ase-dependent cation transport through the lymphocyte membrane by...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::The ability of freshly isolated primary human alveolar epithelial cells (type II pneumocytes) to induce leucocyte migration across an endothelial monolayer was investigated. Three-way factorial analysis of variance (ANOVA) demonstrated that resting alveolar endothelial cells (AEC) could produce detectable quantities o...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01172.x
更新日期:2001-02-01 00:00:00
abstract::The ability to expand tumour-infiltrating lymphocytes in vitro has been greatly enhanced by the use of antigen-independent mechanisms of immune cell costimulation. We have produced human, using the K562 cell line, and murine, using YAC-1 cells, artificial antigen presenting cells (aAPC) and demonstrate that these cell...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01853.x
更新日期:2004-05-01 00:00:00
abstract::Francisella tularensis is a Gram-negative intracellular bacterium that is the causative agent of tularaemia. Concerns regarding its use as a bioterrorism agent have led to a renewed interest in the biology of infection, host response and pathogenesis. A robust T-cell response is critical to confer protection against F...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03387.x
更新日期:2011-03-01 00:00:00
abstract::The delayed-type-hypersensitivity (DTH) reaction observed in mice primed i.v. with low doses of sheep red blood cells is not detectable after priming with large doses. Estimation of the number of DTH mediating cells (DTH-C) was performed by titration (D50 determination) using local adoptive transfer of these cells mix...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract::We produced three murine monoclonal antibodies (mAb) directed against the human T-cell differentiation antigen CD2 (formerly T11) and analysed their epitope specificity by E-rosette inhibition, cross-blocking and proliferation-induction experiments. When added together, two mAb (both IgG1 kappa) were able to induce pr...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract::CD59 is the sole characterized regulator of the complement membrane attack complex in humans. It is very widely and abundantly distributed, being present on all circulating cells, endothelia and epithelia, and in most tissues. CD59 analogues in rodents are distributed similarly. Interest in complement regulation in th...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00623.x
更新日期:1998-11-01 00:00:00