Abstract:
:BLT mice, constructed by surgical implantation of human fetal thymus-liver tissues and intravenous delivery of autologous CD34+ haematopoietic stem cells into adult non-obese diabetic/severe combined immunodeficiency mice, were evaluated for vaccine-induced humoral immune responses. Following engraftment, these mice developed a human lymphoid system; however, the majority of the peripheral human B lymphocytes displayed an immature phenotype as evidenced by surface CD10 expression. Over 50% of the human B cells in the periphery but not in the bone marrow also expressed the CD5 antigen, which is found only infrequently on mature follicular B cells in humans. A single intramuscular immunization with recombinant viral envelope antigens, e.g., HIVgp140 and West Nile Virus envelope proteins, together with the immune stimulatory KLK/ODN1a composition) [corrected] adjuvant resulted in seroconversion characterized by antigen-specific human antibodies predominantly of the IgM isotype. However, repeated booster immunizations did not induce secondary immune responses as evidenced by the lack of class switching and specific IgM levels remaining relatively unchanged. Interestingly, the peripheral CD19+ CD5+ but not the CD19+ CD5- human B lymphocytes displayed a late developing CD27+ IgM+ memory phenotype, suggesting that the CD5+ B-cell subset, previously implicated in 'natural antibody' production, may play a role in the vaccine-induced antibody response. Furthermore, human T lymphocytes from these mice demonstrated suboptimal proliferative responses and loss of co-stimulatory surface proteins ex vivo that could be partially reversed with human interleukin-2 and interleukin-7. Therefore, vaccine-induced immune responses in BLT mice resemble a T-cell-independent pathway that can potentially be modulated in vivo by the exogenous delivery of human cytokines/growth factors.
journal_name
Immunologyjournal_title
Immunologyauthors
Biswas S,Chang H,Sarkis PT,Fikrig E,Zhu Q,Marasco WAdoi
10.1111/j.1365-2567.2011.03501.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
419-33issue
4eissn
0019-2805issn
1365-2567journal_volume
134pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Extracorporeal photochemotherapy (ECP) has been shown to be a potent activator of peripheral blood macrophages because it causes a marked release of macrophage-dependent proinflammatory cytokines, and it is therefore currently considered to be a safe and non-toxic immunomodulatory treatment. On this basis we studied t...
journal_title:Immunology
pub_type: 临床试验,杂志文章
doi:10.1046/j.1365-2567.1997.00325.x
更新日期:1997-09-01 00:00:00
abstract::We studied the generation and metabolism of leukotrienes (LTs) in human lung macrophages obtained from lung tissue of patients with central bronchial carcinoma. By counterflow centrifugation macrophages were enriched with a purity of more than 95-100%. A time and dose dependent generation of LTB4 and LTC4 was determin...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::Among their diverse roles as transcriptional regulators during development and cell fate specification, the RUNX transcription factors are best known for the parts they play in haematopoiesis. RUNX proteins are expressed throughout all haematopoietic lineages, being necessary for the emergence of the first haematopoie...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12535
更新日期:2015-12-01 00:00:00
abstract::Over the last few years, natural killer (NK) cells have been shown to express major histocompatibility complex (MHC) molecules recognizing receptors that are thought to function primarily as negative signalling receptors. Much attention has been focused on the NK cell receptors CD158a (EB6) and CD158b (GI 183), which ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00374.x
更新日期:1997-12-01 00:00:00
abstract::The metabolic consequences of non-lethal complement-membrane attack in neutrophils have been investigated by the measurement of cellular ATP content and functional parameters, including chemotactic and phagocytic responses and the capacity to secrete reactive oxygen metabolites, in cells before and after attack. Immed...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Although it is clear that the CD45 tyrosine phosphatase is required for efficient T-cell activation and T-cell development, the factors that regulate CD45 function remain uncertain. Previous data have indicated that there is an association of CD45 with CD4 and the T-cell receptor (TCR) complex controlled by the variab...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2007.02602.x
更新日期:2007-08-01 00:00:00
abstract::Over the past 10 years we have made great strides in our understanding of T helper cell differentiation, expansion and effector functions. Within the context of T helper type 2 (Th2) cell development, novel innate-like cells with the capacity to secrete large amounts of interleukin-5 (IL-5), IL-13 and IL-9 as well as ...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2011.03497.x
更新日期:2011-12-01 00:00:00
abstract::Silica, or silicon dioxide, has been shown to be toxic for macrophages. This is probably because it damages phagolysosomal membranes, allowing lysosomal enzymes to disrupt the cell. Neutrophils also take up particles such as silica and in addition they contain lysosomes. The purpose of this study was to determine whet...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::Peripheral blood lymphocytes (PBL) from non-immunized rabbits or from rabbits immunized several months previously with a single dose of sheep red blood cells (SRBC) were cultured at several different cell densities in the presence of antigen for 5 days. The cells were then distributed in 10 microliter microcultures at...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-12-01 00:00:00
abstract::We have investigated the potential of CD4+ T cells isolated from Peyer's patches and mesenteric lymph nodes (mucosa-associated lymphoid tissues) and from spleens and peripheral lymph nodes (systemic lymphoid tissues) to secrete a variety of lymphokines. The results show several pronounced differences in the kinetics o...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::The interaction between living groups A, C and G streptococci and IgG Fc was studied using human IgG, IgG Fc and IgG Fc-intermediate (Fci) fragments, chemically modified human IgG and fragment D of staphylococcal protein A (SPA). Diethylpyrocarbonate modification of His or N-acetylimidazole modification of Tyr of huma...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::Previous studies have shown that no cell-mediated immunity against LCM virus-infected cells can be detected in neonatally established LCM virus carrier mice suggesting that they are immunologically tolerant to virally-altered cell membrane antigens. In this communication experiments are described aimed at analyzing th...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::Following prescapular lymphadenectomy, the cellular composition of afferent (peripheral) lymph from eight out of 13 calves in which the afferent and efferent lymphatic ducts had anastomosed was examined using monoclonal antibodies (mAb) specific for bovine lymphoid populations. Afferent lymph leucocytes (ALL) consiste...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::In recent years, nitric oxide (NO), a gas previously considered to be a potentially toxic chemical, has been established as a diffusible universal messenger that mediates cell-cell communication throughout the body. Constitutive and inducible NO production regulate numerous essential functions of the gastrointestinal ...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2004.01984.x
更新日期:2004-12-01 00:00:00
abstract::After a primary infection of congenitally athymic nu/nu mice with Strongyloides ratti, worms were not expelled and the number of intestinal mucosal mast cells (MMC) remained at a low level. When S. ratti-infected nu/nu mice were treated by repetitive injections of semi-purified IL-3 from Day 5 to Day 10 post-infection...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract::Thoracic duct lymphocytes (TDL) from (AS2 x AS)F1 rats previously injected with human serum albumin (HSA) were transferred to 900 r irradiated syngeneic recipients which were challenged with various doses of soluble HSA (s-HSA). TDL from partially tolerant rats, which were deficient in high avidity B cells, produced a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-10-01 00:00:00
abstract::We have previously reported that human granulocyte-macrophage colony-stimulating factor (hGM-CSF) causes a stage-specific inhibition of T-cell receptor (TCR) alphabeta cell development in the thymus of transgenic mice constitutively expressing the hGM-CSF receptor. Since it has been reported that the addition of inter...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01402.x
更新日期:2002-06-01 00:00:00
abstract::Following exposure of mice to contact sensitizing chemicals, dendritic cells (DC) rapidly accumulate in the draining lymph nodes. A proportion, at least, of the DC which arrive in the nodes bear significant amounts of antigen and are derived from epidermal Langerhans' cells (LC). It is of interest that although LC are...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::We investigated the individual CD8+ populations with natural killer (NK) cell markers (NK-type T cell); CD56 single positive (CD56)-T cells, CD56/CD57 double positive (DP)-T cells and CD57 single positive (CD57)-T cells in the peripheral blood. All NK-type T-cell populations expressed CD122 and intermediate levels of ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01575.x
更新日期:2003-02-01 00:00:00
abstract::Interleukin-19 (IL-19) plays an important role in asthma by stimulating T helper type 2 (Th2) cytokine production. Interestingly, IL-4, a key Th2 cytokine, in turn up-regulates IL-19 expression in bronchial epithelial cells, so forming a positive feedback loop. In atopic dermatitis (AD), another Th2 disease closely re...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12339
更新日期:2014-12-01 00:00:00
abstract::A subpopulation of human peripheral blood natural killer (NK) cells, defined by sedimentation at Percoll high buoyant densities (P greater than 1.0635-1.0640 g/ml) and unresponsiveness to interleukin-2 (IL-2), contained two distinct populations based on the intensity of CD16 (FcR gamma III) expression, namely CD16dim ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Interleukin 27 (IL-27) has been identified as a potent cytokine in the differentiation of type 1 regulatory T (Tr1) cells through interactions with several key elements, including transcription factors such as aryl hydrocarbon receptor and IL-21. Autocrine production of IL-21 is known to be important for maintaining I...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12789
更新日期:2017-11-01 00:00:00
abstract::Free fatty acids (FFA) were tested for their effects on in vitro thymus-dependent (TD) and thymus-independent (TI) immune responses. Murine T cells proliferating in one-way mixed leucocyte reactions (MLR) were extremely sensitive to inhibition by exogenous stearic acid (18:0) but were only moderately affected by oleic...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-12-01 00:00:00
abstract::2B4 (CD244) is a member of the CD2 subset of the immunoglobulin superfamily and functions as a triggering molecule on natural killer (NK) cells. Previously, we have found that 2B4-mediated activation of NK cells involves complex interactions involving LAT, Ras, Raf, ERK and p38 and that cytolytic function and cytokine...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01662.x
更新日期:2003-07-01 00:00:00
abstract::The role of the mouse homocytotropic antibodies in passive cutaneous anaphylaxis reaction was investigated. One class of antibody was heat stable, detected at 2 h but not at 48 h after passive transfer, and belonged to a sublcass of mouse IgG. The other was heat labile, detected at 2 h and 48 h after passive transfer,...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-04-01 00:00:00
abstract::Human haematopoietic progenitor/stem cells (HPCs) differentiate into functional T cells in the thymus through a series of checkpoints. A convenient in vitro system will greatly facilitate the understanding of T-cell development and future engineering of therapeutic T cells. In this report, we established a lentiviral ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.03013.x
更新日期:2009-09-01 00:00:00
abstract::Dendritic cells (DC) are the initiators of immune responses and are present in most tissues in vivo. To generate myeloid DC from monocytes (MoDC) in vitro the necessary cytokines are granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Using degenerated primers delineated from other spec...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02319.x
更新日期:2006-04-01 00:00:00
abstract::Monocyte-derived macrophages (MDMs) generated from peripheral blood monocytes are widely used to model human macrophages for in vitro studies. However, the possible impact of different isolation methods on the resulting MDM phenotype is poorly described. We aimed to investigate the effects of three commonly used monoc...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13125
更新日期:2020-01-01 00:00:00
abstract::CD45 is known to regulate signalling through many different surface receptors in diverse haemopoietic cell types. Here we report for the first time that CD45-/- bone marrow dendritic cells (BMDC) are more activated than CD45+/+ cells and that tumour necrosis factor (TNF) and interleukin-6 (IL-6) production by BMDC and...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02363.x
更新日期:2006-06-01 00:00:00
abstract::Excessive and dysregulated inflammation is known to contribute to disease progression. HSP90 is an intracellular chaperone known to regulate inflammatory processes including the NLRP3 inflammasome and secretion of the pro-inflammatory cytokine interleukin(IL)-1β. Here, primarily using an in vitro inflammasome ASC spec...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13267
更新日期:2021-01-01 00:00:00