Abstract:
:The metabolic consequences of non-lethal complement-membrane attack in neutrophils have been investigated by the measurement of cellular ATP content and functional parameters, including chemotactic and phagocytic responses and the capacity to secrete reactive oxygen metabolites, in cells before and after attack. Immediately after non-lethal complement attack, cellular ATP content was reduced by more than 75%, although lactate dehydrogenase content was unaltered. Energy-requiring cell functions were similarly depressed. Incubation of cells in nutrient medium rapidly restored cell-energy stores and functions, demonstrating the completeness of recovery. Fluorescence-activated cell-sorter studies demonstrated that cells undergoing non-lethal complement attack underwent a reversible cell swelling, the cell diameter rapidly increasing from an average of 8.5 micron to 12 micron, then gradually shrinking back to a final average diameter of 8.2 micron. The results indicate that although non-lethal complement-membrane attack causes both metabolic and physical changes in neutrophils, these effects are transient and full functional recovery occurs.
journal_name
Immunologyjournal_title
Immunologyauthors
Morgan BPsubject
Has Abstractpub_date
1988-01-01 00:00:00pages
71-7issue
1eissn
0019-2805issn
1365-2567journal_volume
63pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Induction of autoimmune antibodies against antigens of glomerular basement membrane (GBM) was studied in nine inbred strains of rats each with a different major histocompatibility complex H-1. Brown-Norway (BN) (H-1n), Lewis (H-1(1)), PVG/c (H-1c), AS2 (H-1f), AVN (H-1a), BD V (H-1d), DA (H-1a) and F344 (H-1(1)) rats ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::To evaluate the T-cell large-scale differential gene expression in systemic lupus erythematosus (SLE) patients presenting with glomerulonephritis we studied SLE patients before and after immunosuppressive treatment. Large-scale gene expression of peripheral blood mononuclear T cells was evaluated using cDNA microarray...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01929.x
更新日期:2004-09-01 00:00:00
abstract::Thoracic duct lymphocytes (TDL) from (AS2 x AS)F1 rats previously injected with human serum albumin (HSA) were transferred to 900 r irradiated syngeneic recipients which were challenged with various doses of soluble HSA (s-HSA). TDL from partially tolerant rats, which were deficient in high avidity B cells, produced a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-10-01 00:00:00
abstract::Clonal deletion of T cells capable of recognizing both host-type Mls and donor-type Mls occurred in the peripheral mature T-cell pool in radiation bone marrow chimeras of two H-2-compatible Mls-disparate strain combinations of AKR/J(H-2k,Thy-1.1,Mls-1a) and C3H/He(H-2k,Thy-1.2,Mls-1b). In order to determine further th...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::The plaque-forming cell (PFC) responses to sheep red blood cell (SRBC) dinitrophenyl-lysine-Ficoll (DNP-lys-Ficoll), and dinitrophenylated bovine serum albumin (DNP-BSA) have been studied in both germ-free and conventionally reared ICR mice. In germ-free mice, the IgG response to SRBC and the IgM and IgG responses to ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Natural killer (NK) cells, which can exert early and powerful anti-tumour and anti-viral responses, are important components of the innate immune system. DNAX accessory molecule-1 (DNAM-1) is an activating receptor molecule expressed on the surface of NK cells. Recent findings suggest that DNAM-1 is a critical regulat...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12516
更新日期:2015-11-01 00:00:00
abstract::Cytokines and their receptors represent key targets for therapeutic intervention. Ligands are being used to supplement cell numbers that become depleted as a result of disease (organ failure, infection) or subsequent disease treatments (i.e. chemotherapy). Conversely, the inhibition of target cell binding by cytokines...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2004.01886.x
更新日期:2004-06-01 00:00:00
abstract::During acute Trypanosoma cruzi infection in mice, many leucocytes undergo apoptosis. Although apoptosis has been ascribed to increased levels of nitric oxide (NO) and Fas-FasL interaction, the importance of this phenomenon in modulating the host response against T. cruzi is unknown. Herein, the role of NO- and Fas-Fas...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01216.x
更新日期:2001-05-01 00:00:00
abstract::Histamine not only mediates immediate allergic reactions, it also regulates cellular immune responses. H4R is the most recently identified histamine receptor (HR). In the present study, we examined the in vitro effect of histamine and H4R agonists on the responses of human T cells to purified protein derivative from M...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2007.02574.x
更新日期:2007-06-01 00:00:00
abstract::We have previously shown that chlorpromazine (CPZ) inhibits tumour necrosis factor (TNF) production and protects against endotoxic shock in mice. In this paper we investigated the effect of pretreatment with CPZ, 4 mg/kg i.p. 30 min before, compared with dexamethasone (DEX; 3 mg/kg) on the induction of other endotoxin...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Different cell types, including dendritic cells, macrophages and Ia+ B cells, have been described to present soluble antigen (Ag) to T-cell hybridomas. However, it is still not clear whether these different cell types can act as antigen-presenting cells (APC) for complex and insoluble Ag such as viral particles. Using...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease with high incidence and mortality in South East Asia and northern Australia. To date there is no protective vaccine and antibiotic treatment is prolonged and not always effective. Most people living in endemic areas have been expose...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12709
更新日期:2017-05-01 00:00:00
abstract::The Ager assay was adapted to a porcine lymphocyte-rat high endothelial cell (HEC) system. Using this in vitro assay, the role of porcine CD44 in lymphocyte binding to HEC was examined. The results show that the presence of soluble CD44 molecules did not inhibit the binding of porcine lymphocytes to the cultured rat H...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Feeding antigens induces an immunological unresponsiveness termed oral tolerance but under some conditions, for example following the administration of cyclophosphamide (CY), immunity can be induced. These observations have usually been made by studying antibody production and delayed hypersensitivity with little atte...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::A decrease in the number of dendritic cells (DCs) is a major cause of post-sepsis immunosuppression and opportunistic infection and is closely associated with poor prognosis. Increasing the number of DCs to replenish their numbers post sepsis can improve the condition. This therapeutic approach could improve recovery ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12423
更新日期:2015-05-01 00:00:00
abstract::Polystyrene surfaces coated with proteins at alkaline pH were found to be useful to investigate the stimulation of neutrophils independently of phagocytosis. Human neutrophils, when exposed to appropriate stimuli, release superoxide anion (O2-). We have measured superoxide dismutase-inhibitable cytochrome c reduction ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract::Human granulocyte-macrophage colony-stimulating factor (GM-CSF) reporter constructs containing up to 3.3 kb of upstream promoter sequence were transiently transfected into both Jurkat and HUT78 human T-cell lines. In Jurkat cells, stimulation with phorbol 12-myristate 13-acetate (PMA) plus phytohemaglutinin (PHA) prod...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.0019-2805.2004.01833.x
更新日期:2004-04-01 00:00:00
abstract::NKG2D is one of the best characterized activating receptors and is expressed on natural killer cells and on various T-cell subsets. This receptor recognizes several different ligands that are induced by cellular stresses. In this review, we described the mechanisms controlling the expression of NKG2D ligands, with the...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2009.03147.x
更新日期:2009-09-01 00:00:00
abstract::HIV infection is associated with increased representation of T cells bearing an activated, memory (CD45RO+) phenotype. Although administration of antiretroviral agents and interleukin-2 (IL-2) augment depleted CD4+ T-cell numbers, such therapies have been preferentially beneficial for CD45RO+ T cells. Interleukin-15 (...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00807.x
更新日期:1999-06-01 00:00:00
abstract::Adoptive transfer of T-cells is a promising therapy for many cancers. To enhance tumour recognition by T-cells, chimeric antigen receptors (CARs) consisting of signalling domains fused to receptors that recognize tumour-associated antigens can be expressed in T-cells. While CAR T-cells have shown clinical success for ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13187
更新日期:2020-07-01 00:00:00
abstract::Previously, a putative immunosuppressant-coding gene was identified from a complementary DNA library derived from the salivary glands of partially-fed Haemaphysalis longicornis. Using real-time polymerase chain reaction, the gene was shown to be predominantly expressed during blood feeding with the site of expression ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02890.x
更新日期:2009-02-01 00:00:00
abstract::Mice homozygous for the gld (generalized lymphoproliferative disease) and lpr (lymphoproliferation) mutations display similar autoimmune and lymphoproliferative diseases. Both result from defective apoptosis, the targets of the lpr and gld mutations being the genes for, respectively, an apoptosis-signalling receptor [...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::The ultimate outcome of T-cell recognition of peptide-major histocompatibility complex (MHC) complexes is determined by the molecular context in which antigen presentation is provided. The paradigm is that, after exposure to peptides presented by steady-state dendritic cells (DCs), inhibitory signals dominate, leading...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03216.x
更新日期:2010-05-01 00:00:00
abstract::Different kinds of IgM antibodies were tested for their activity in antibody-dependent cellular cytotoxicity (ADCC): firstly an anti-benzylpenicilloyl (BPO) IgM antibody from immune rabbit serum purified by affinity, ion exchange, and molecular-sieving chromatography, secondly two monoclonal rat anti-BPO IgM antibodie...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::In our efforts to identify products that might be used for active immunotherapy in human immunodeficiency virus (HIV) infection, we have studied synthetic peptides derived from the CD4 attachment site of gp120. Two peptides have emerged with particularly interesting properties. The first (B138) is linear and spans the...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::The subcutaneous (s.c.) injection of sheep red blood cells (SRBC) without adjuvant into mice preferentially induced delayed hypersensitivity (DH) reaction, as measured by footpad swelling, while intraperitoneal (i.p.) measured by the haemagglutinin test. Under these conditions, the properties of the helper activities...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-05-01 00:00:00
abstract::The level of circulating thymic factor (FTS) tested by its action on spleen rosette-forming cells from adult thymectomized mice, has been shown to be stable in young animals. This stability suggests a regulatory mechanism. An approach of this regulation has been attempted by disrupting the FTS level either by neonatal...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::We have recently described a bone marrow culture system which is able to maintain a portion of the precursor-T (pre-T) cell compartment of adult murine marrow in vitro, in the presence of interleukin-3 (IL-3), for at least 2 weeks. However, because growth in IL-3 might also induce the differentiation of the pre-T cell...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::The biological effects of three thymosin fraction 5 polypeptides, designated as beta 10, beta 4, and alpha 1 were tested in the MLR of mouse splenocytes, lymph node cells and thymocytes against syngeneic or allogeneic stimulators. It was found that all three polypeptides, after in vivo and in vitro treatment of the re...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract::Porcine alveolar macrophages (AM) metabolize leukotriene D4 (LTD4) to leukotriene E4 (LTE4). In the present study, the ability of a fluid-phase AM stimulus (A23187) and a phagocytic stimulus (opsonized zymosan) to augment LTD4 metabolism was examined. Both stimuli increased the release of superoxide (O-2) anions. Howe...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00