Abstract:
:The effect of invasive bacteria on the release of proinflammatory mediators (oxygen radicals, leukotriene release) from human polymorphonuclear neutrophils was studied. Bacterial stimuli were used including genetically cloned invasive Yersinia enterocolitica strains 108-P (bearing the phagocytosis-resistance plasmid) and 108-C (plasmidless variant), Listeria monocytogenes [SLCC 5779 (inv-) and NCTC 7973 (inv+)] as well as an Escherichia coli K 12 strain (pRI 203) in which the inv gene of Y. pseudotuberculosis was cloned. When human polymorphonuclear granulocytes were studied as target cells the inv+ as well as the inv- strains were phagocytosed to a comparable amount with the exception of the L. monocytogenes strain (inv+). Among the invasive strains E. coli HB 101 (pRI 203) was the most active to trigger polymorphonuclear leucocytes (PMN) for oxygen radical production. Preincubation of the cells with bacteria and subsequent stimulation with the Ca ionophore A23187 or opsonized zymosan suppressed the chemiluminescence response to a different degree. The various bacterial strains did not induce leukotriene release from endogenous arachidonic acid. Subsequent stimulation of the infected cells with Ca ionophore or opsonized zymosan led to an altered pattern of the combined amounts of leukotriene B4 (LTB4), 20-OH- and 20-COOH-LTB4 as well as the ratio of LTB4 versus 20-OH and 20-COOH-LTB4. Infection of the cells also reduced strain dependently the number of LTB4-receptor sites. Our data suggest that bacterial uptake modulates the inflammatory response of granulocytes (e.g. chemiluminescence response, leukotriene generation).
journal_name
Immunologyjournal_title
Immunologyauthors
Gröne M,Scheffer J,König Wkeywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
400-7issue
3eissn
0019-2805issn
1365-2567journal_volume
77pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Tumour necrosis factor-alpha (TNF-alpha) stimulated invasion by mouse T-cell hybridomas and cytotoxic T-lymphocyte clones into rat embryo fibroblast monolayers. The effect on these highly invasive cells was limited: invasion was stimulated maximally to 130% of controls. However, when cells were pretreated with pertuss...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
abstract::Cells expressing indoleamine 2,3-dioxygenase (IDO), an enzyme which catabolizes tryptophan, prevent T-cell proliferation in vitro, suppress maternal antifetal immunity during pregnancy and inhibit T-cell-mediated responses to tumour-associated antigens. To examine the mechanistic basis of these phenomena we activated ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01526.x
更新日期:2002-12-01 00:00:00
abstract::Mice fed a protein-deficient diet containing 2% ovalbumin for 6 weeks (PD) show a reduced spleen IgA plaque-forming cell (PFC) response after oral immunization with sheep red blood cells (SRBC), compared to the response of mice fed a control diet containing 20% ovalbumin (C). This reduced IgA PFC response was partiall...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::Autoimmune diabetes is predominated by a T helper 1 (Th1) response at the expense of an impaired Th2 response. Although T cells producing Th2 cytokines are generally thought to counter a Th1 response, there have been reports of Th2 T-cell clones with pathogenic activity, including one previously reported by us in whic...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2007.02715.x
更新日期:2008-01-01 00:00:00
abstract::Dengue virus (DENV) infection causes dengue fever, dengue haemorrhagic fever, or dengue shock syndrome. Mast cells have been speculated to play a role in DENV disease although their precise roles are unclear. In this study, we used mast cell-deficient Kit(W-sh/W-sh) mice to investigate the involvement of mast cells af...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12492
更新日期:2015-09-01 00:00:00
abstract::Isolated human mononuclear cells were fractionated according to their membrane characteristics or physical properties. Adherent cells were depleted by filtration through glass columns; phagocytic cells were removed by iron treatment and cell subpopulations capable of forming rosettes with sheep erythrocytes (E), eryth...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::Herpes simplex virus (HSV) encodes a protein, glycoprotein C (gC), which binds to the third complement component, the central mediator of complement activation. In this study the structural and functional relationships of gC from HSV type 1 (HSV-1) and known human complement regulatory proteins factor H, properdin, fa...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::with AgB-primed B or T cells and injected into syngeneic X-irradiated recipients. Anti-THF and anti-Eid purified by an AgB-specific T suppressor factor (TSF) affi-gel adsorbent retain their ability to specifically initiate [3H]-thymidine incorporation of AgB-primed T cells. The data indicate that both anti-THF and ant...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::Mouse lymph node cells cultured with Concanavalin A (Con A) in serum-free medium containing 59Fe-transferrin took up 59Fe more rapidly than cells cultured without Con-A. Uptake of iron commenced rapidly and preceded the onset of DNA synthesis in stimulated cells. Total uptake of transferrin during culture was much low...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::In the rat strain combination of DA into PVG, an orthotopic liver graft has the ability to abrogate an existing state of sensitization against donor (DA) antigens. Fifty-four percent of PVG rats sensitized against DA by skin grafting accepted a DA liver graft permanently, and about half of these became systemically to...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::We have established lines and clones of helper T cells specific for the antigen fowl gamma globulin (FGG) and maintained these cells in continuous proliferation in vitro for up to 8 months. During this period the specific helper activity was assessed in vitro in T cell-dependent anti-hapten responses. These pure popul...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::The effector CD8(+) T cells recognize major histocompatibility complex (MHC) class I binding altered self-peptides expressed in tumour cells. Although the requirement for CD4(+) T helper type 1 (Th1) cells in regulating CD8(+) T cells has been documented, their target epitopes and functional impact in antitumour respo...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02262.x
更新日期:2006-01-01 00:00:00
abstract::The role of reactive nitrogen intermediates (RNI) in the antimicrobial activities of neutrophils from various mammalian species is unclear. However, it has been reported that rodent neutrophils possess the inducible form of nitric oxide synthase and that inflammatory neutrophils from rats produce potentially antimicro...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Cytotoxic T-lymphocytes (CTL) play an important role in the control of human immunodeficiency virus (HIV) and of human cytomegalovirus (HCMV) infection. Following highly active antiretroviral therapy (HAART), most studies have demonstrated a decline in the frequency of HIV-specific CTL. We analysed the effect of HAART...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02334.x
更新日期:2006-05-01 00:00:00
abstract::In recent years, nitric oxide (NO), a gas previously considered to be a potentially toxic chemical, has been established as a diffusible universal messenger that mediates cell-cell communication throughout the body. Constitutive and inducible NO production regulate numerous essential functions of the gastrointestinal ...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2004.01984.x
更新日期:2004-12-01 00:00:00
abstract::Trypanosoma cruzi, the causative agent of Chagas' disease, suppresses immune responses during the acute phase and has been shown to induce multiple cellular alterations in activated human T lymphocytes. However, no information is available regarding the effects of this parasite on human B cells. Using an in vitro cult...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::Programmed death-1 (PD-1) plays an important role in protecting against inflammation and myocyte damage in T-cell-mediated myocarditis. To understand whether fibrinogen-like protein-2 (FGL2) can affect the role of the PD-1/PD-L1 pathway in experimental autoimmune myocarditis (EAM), we investigated cardiac function in ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12837
更新日期:2018-02-01 00:00:00
abstract::Previously, a putative immunosuppressant-coding gene was identified from a complementary DNA library derived from the salivary glands of partially-fed Haemaphysalis longicornis. Using real-time polymerase chain reaction, the gene was shown to be predominantly expressed during blood feeding with the site of expression ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02890.x
更新日期:2009-02-01 00:00:00
abstract::This study investigates the effect of feeding fermented milks with Lactobacillus casei, Lactobacillus acidophilus and a mixture of both micro-organisms on the specific and non-specific host defence mechanisms in Swiss mice. Animals fed with fermented milk for 8 days (100 micrograms/day) showed an increase in both phag...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Evidence is accumulating that dietary iodine intake is an important modulator of autoimmune thyroid reactions. To study this role of iodine intake further, female Wistar rats were kept on an enriched iodine diet (EID, iodine intake 100 micrograms iodine/day) for a period of up to 18 weeks. Control rats were either on ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Culture dishes precoated with thin layers of acid soluble rat tail collagen simplify conditions necessary to obtain in vitro high IgG anti-DNP responses from primed and boosted mice. In contrast to Mishell-Dutton type cultures the use of collagen precludes the need to continually rock cultures and to add nutrients. Re...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::The cell-cell interactions for CD23 expression and soluble (s)CD23 release from peripheral blood lymphocytes (PBL), as well as purified cells (B, T cells, monocytes) of atopic donors, were studied. Cells either stimulated combined and subsequently separated or stimulated after separation were analysed. IL-4, IL-2, phy...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::Effect of macrophage culture fluid (MF) on thymic independent (TI) antibody responses was examined. MF potentiated antibody responses of spleen cells to dinitrophenyl (DNP)-Ficoll and DNP-liposome, TI-2 antigens, but not to trinitrophenyl (TNP)-BA and TNP-LPS, TI-1 antigens. The enhancing effect of MF on the anti-DNP-...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-09-01 00:00:00
abstract::Colorectal cancer is considered a non-immunogenic malignany. One strategy to augment the immunogenicity of such tumours is represented by the expression of costimulatory molecules by gene transfer. Using transfected variants of the human colorectal cancer cell line SW480 we tested various costimulatory molecules (CD80...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00450.x
更新日期:1998-03-01 00:00:00
abstract::Antigen-induced allergic airway inflammation is mediated by T helper type 2 (Th2) cells and their cytokines, but the mechanism that initiates the Th2 immunity is not fully understood. Recent studies show that basophils play important roles in initiating Th2 immunity in some inflammatory models. Here we explored the ro...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12240
更新日期:2014-06-01 00:00:00
abstract::The interaction between living groups A, C and G streptococci and IgG Fc was studied using human IgG, IgG Fc and IgG Fc-intermediate (Fci) fragments, chemically modified human IgG and fragment D of staphylococcal protein A (SPA). Diethylpyrocarbonate modification of His or N-acetylimidazole modification of Tyr of huma...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::A murine model was used to study the respective roles of L3T4+ and Lyt-2+ T cells in protection against Chlamydia psittaci. Donor mice were intravenously (i.v.) infected with 1 x 10(5) plaque-forming units (PFU) per mice of live C. psittaci. One month after inoculation, splenic cells from donors were transferred into ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::The negative role exerted by cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) in the regulation of T-cell activity, as induced by T-cell receptor (TCR)/CD3 and CD28 costimulation, has been widely described. In the present work we investigated the role of CTLA-4 in the control of cell activation, as induced by cost...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01493.x
更新日期:2002-10-01 00:00:00
abstract::Mice homozygous for the gld (generalized lymphoproliferative disease) and lpr (lymphoproliferation) mutations display similar autoimmune and lymphoproliferative diseases. Both result from defective apoptosis, the targets of the lpr and gld mutations being the genes for, respectively, an apoptosis-signalling receptor [...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::The role of anti-idiotypic subclass-specific antibodies was analysed in the regulation of the immune response to BCG in the guinea-pig. The idiotypes to BCG were separated into subclasses and anti-idiotypes were carried out by immunizing with the purified IgG1 and IgG2 anti-idiotypes. The in vivo T cell response was r...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00