Abstract：
:Platelet-derived growth factor (PDGF), as purified from fresh human platelets, is a protein of relative molecular mass (Mr) 30,000 composed of two disulphide-linked subunit chains of similar size, named A and B (ref. 1). The dimer structure of PDGRF seems to be important for its biological effects, as reduction irreversibly inactivates the factor; it is not known, however, whether PDGF exists as a heterodimer or as a mixture of homodimers. Amino-acid sequence analysis has revealed that the A- and B-chains of human PDGF are related to each other, and that the B-chain is almost identical to part of the v-sis gene product of simian sarcoma virus (SSV). There is experimental evidence that a PDGF-like protein is indeed operational in SSV-induced transformation and the biologically active v-sis product is probably structurally similar to a putative dimer of PDGF B-chains. PDGF-like growth factors and/or a 4.2-kilobase (kb) c-sis transcript are present in several transformed mammalian cell lines and in certain nontransformed cells; cloned c-sis complementary DNA from human T cells transformed with human T-lymphotropic virus (HTLV) or from human endothelial cells contains the coding sequence for a putative PDGF B-chain precursor, but apparently lacks PDGF A-chain sequences. We have previously partially purified and characterized a PDGF-like growth factor from U-2 OS cells (osteosarcoma-derived growth factor, ODGF) and shown that this factor has structural, functional and immunological characteristics in common with PDGF. We describe here a procedure for the preparation of homogeneous ODGF, and provide evidence that this factor, which binds to the PDGF receptor, has a structure similar to a homodimer of PDGF A-chains.
journal_name
Naturejournal_title
Natureauthors
Heldin CH,Johnsson A,Wennergren S,Wernstedt C,Betsholtz C,Westermark Bdoi
10.1038/319511a0subject
Has Abstractpub_date
1986-02-06 00:00:00pages
511-4issue
6053eissn
0028-0836issn
1476-4687journal_volume
319pub_type
杂志文章相关文献
NATURE文献大全abstract：:Proteolysis is essential for the execution of many cellular functions. These include removal of incorrectly folded or damaged proteins, the activation of transcription factors, the ordered degradation of proteins involved in cell cycle control, and the generation of peptides destined for presentation by class I molecu...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/33443
更新日期：1998-04-09 00:00:00
abstract：:Osteoarthritis-the most common form of age-related degenerative whole-joint disease1-is primarily characterized by cartilage destruction, as well as by synovial inflammation, osteophyte formation and subchondral bone remodelling2,3. However, the molecular mechanisms that underlie the pathogenesis of osteoarthritis are...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-0920-1
更新日期：2019-02-01 00:00:00
abstract：:The regulation of intracellular pH (pHi) is essential for normal cell function, and controlled changes in pHi may play a central role in cell activation. Sodium-dependent Cl-HCO3 exchange is the dominant mechanism of pHi regulation in the invertebrate cells examined, and also occurs in mammalian cells. The transporter...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/332262a0
更新日期：1988-03-17 00:00:00
abstract：:The human alpha-interferon (IFN-alpha) gene family consists of at least 14 potentially functional non-allelic members; the amino acid sequences they encode differ from each other by up to approximately 20% of their residues. Human IFN-beta, which is encoded by a single gene, is distantly related to the IFN-alpha famil...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/313698a0
更新日期：1985-02-21 00:00:00
abstract：:Transgenic mice bearing the cellular myc oncogene coupled to the immunoglobulin mu or kappa enhancer frequently develop a fatal lymphoma within a few months of birth. Since the tumours represent represent both immature and mature B lymphocytes, constitutive c-myc expression appears to be highly leukaemogenic at severa...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/318533a0
更新日期：1985-12-12 00:00:00
abstract：:With only a few exceptions that are well understood, conventional superconductivity does not coexist with long-range magnetic order (for example, ref. 1). Unconventional superconductivity, on the other hand, develops near a phase boundary separating magnetically ordered and magnetically disordered phases. A maximum in...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature04571
更新日期：2006-03-02 00:00:00
abstract：:During formation of the neuromuscular junction, acetylcholine receptors in the endplate membrane become metabolically stabilized under neural control, their half-life increasing from about 1 day to about 10 days. The metabolic stability of the receptors is regulated by the electrical activity induced in the muscle by ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/349337a0
更新日期：1991-01-24 00:00:00
abstract：:Human T-cell leukaemias and lymphomas associated with the human T-cell leukaemia viruses (HTLV) are invariably neoplasms of cells with mature T-lymphocyte phenotype. Epstein-Barr virus-transformed B- lymphoycte lines which are productively infected with HTLV may be isolated from patients with HTLV malignancies, but no...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/309276a0
更新日期：1984-05-17 00:00:00
abstract：:Visual transduction in vertebrate photoreceptors is thought to involve a diffusible internal transmitter which links photon absorption in disk membranes to the conductance decrease in the plasma membrane of the outer segment. The desensitisation of the photoresponse which occurs during and after illumination may also ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/283085a0
更新日期：1980-01-03 00:00:00
abstract：:To identify cis-acting DNA elements involved in the activation of the alpha-fetoprotein gene during differentiation, modified copies of the gene were introduced into murine F9 embryonal carcinoma cells. The differentiation of the transformants to either parietal or visceral endoderm was accompanied by induction of the...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/310562a0
更新日期：1984-08-16 00:00:00
abstract：:Diapycnal mixing (across density surfaces) is an important process in the global ocean overturning circulation. Mixing in the interior of most of the ocean, however, is thought to have a magnitude just one-tenth of that required to close the global circulation by the downward mixing of less dense waters. Some of this ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12432
更新日期：2013-09-19 00:00:00
abstract：:Collections in early Middle Miocene deposits at Pasalar in Turkey have yielded a very rich fauna. Included in this are two hominoid species referred here to Sivapithecus darwini (Abel) 1902 and Ramapithecus wickeri (Leakey) 1962. These are both more primitive morphologically and earlier in time than other species of t...
journal_title：Nature
pub_type： 历史文章,杂志文章
doi：10.1038/268699a0
更新日期：1977-08-25 00:00:00
abstract：:Control of protein synthesis is associated with changes in the ratio of intracellular Na+ to K+ in the cultured embryonic chick lens. Correlations of intracellular Na+/K+ ratios with crystallin synthesis and cataract formation in vitro suggest that the Na+/K+ ratio may have an important role in the regulation of prote...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/270406a0
更新日期：1977-12-01 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/d41586-019-00752-3
更新日期：2019-03-01 00:00:00
abstract：:High-temperature superconductivity is achieved by doping copper oxide insulators with charge carriers. The density of carriers in conducting materials can be determined from measurements of the Hall voltage--the voltage transverse to the flow of the electrical current that is proportional to an applied magnetic field....
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01890
更新日期：2003-08-21 00:00:00
abstract：:A plume of water vapour escapes from fissures crossing the south polar region of the Saturnian moon Enceladus. Tidal deformation of a thin surface crust above an internal ocean could result in tensile and compressive stresses that would affect the width of the fissures; therefore, the quantity of water vapour released...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature07542
更新日期：2008-11-27 00:00:00
abstract：:Thewissen et al. describe new fossils from India that apparently support a phylogeny that places Cetacea (that is, whales, dolphins, porpoises) as the sister group to the extinct family Raoellidae, and Hippopotamidae as more closely related to pigs and peccaries (that is, Suina) than to cetaceans. However, our reanaly...
journal_title：Nature
pub_type： 评论,杂志文章
doi：10.1038/nature07776
更新日期：2009-03-19 00:00:00
abstract：:The recently emerged fields of metamaterials and transformation optics promise a family of exciting applications such as invisibility, optical imaging with deeply subwavelength resolution and nanophotonics with the potential for much faster information processing. The possibility of creating optical negative-index met...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature09278
更新日期：2010-08-05 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 社论
doi：10.1038/d41586-018-07410-0
更新日期：2018-11-01 00:00:00
abstract：:Monocular lid suture during the sensitive period early in the life of a kitten disrupts normal development of inputs from the two eyes to the visual cortex, causing a decrease in the fraction of cortical cells responding to the deprived eye. Such an ocular dominance shift has been assumed to depend on patterned visual...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/324154a0
更新日期：1986-11-13 00:00:00
abstract：:Positron-emission tomography (PET) can localize functions of the human brain by imaging regional cerebral blood flow (CBF) during voluntary behaviour. Functional brain mapping with PET, however, has been hindered by PET's poor spatial resolution (typically greater than 1 cm). We have developed an image-analysis strate...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/323806a0
更新日期：1986-10-30 00:00:00
abstract：:In the absence of extraneously added template, standard preparations of Q beta replicase spontaneously synthesize RNA in vitro, possibly as a result of RNA contamination. Using special enzyme purifications, Sumper and Luce presented evidence that self-replicating RNA not present ab initio can grow out of 'template-fre...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/321089a0
更新日期：1986-05-01 00:00:00
abstract：:It is thought that in skeletal muscle excitation-contraction (EC) coupling, the release of Ca2+ from the sarcoplasmic reticulum is controlled by the dihydropyridine (DHP) receptor in the transverse tubular membrane, where it serves as the voltage sensor. We have shown previously that injection of an expression plasmid...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/346567a0
更新日期：1990-08-09 00:00:00
abstract：:The neurotrophin receptor TrkB is essential for normal function of the mammalian brain. It is expressed in three splice variants. Full-length receptors (TrkB(FL)) possess an intracellular tyrosine kinase domain and are considered as those TrkB receptors that mediate the crucial effects of brain-derived neurotrophic fa...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01983
更新日期：2003-11-06 00:00:00
abstract：:An attempt is made to understand the characteristics of pulsar slowdown in terms of various possible emission mechanisms. Though magnetic-dipole radiation seems to be dominant, the deviations from a pure dipolar decay are large compared with errors in measurement and indicate the presence of more complicated braking m...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/228445a0
更新日期：1970-10-31 00:00:00
abstract：:Whereas short-term memory lasts from minutes to hours, long-term memory (LTM) can last for days or even an entire lifetime. LTM generally forms after spaced repeated training sessions and involves the regulation of gene expression, thereby implicating transcription factors in the initial steps of LTM establishment. Ho...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02726
更新日期：2004-07-22 00:00:00
abstract：:Superantigens such as the staphylococcal enterotoxins bind to major histocompatibility complex (MHC) class II molecules and activate T cells through a specific interaction between the V beta region of the T-cell antigen receptor (TCR) and the toxin. The TCR beta-chain alone is sufficient to produce the interaction wit...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/359841a0
更新日期：1992-10-29 00:00:00
abstract：:Brain-derived neurotrophic factor (BDNF) and other neurotrophins have a vital role in the development of the rat and mouse nervous system by influencing the expression of many specific genes that promote differentiation, cell survival, synapse formation and, later, synaptic plasticity. Although nitric oxide (NO) is kn...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature07238
更新日期：2008-09-18 00:00:00
abstract：:53BP1 governs a specialized, context-specific branch of the classical non-homologous end joining DNA double-strand break repair pathway. Mice lacking 53bp1 (also known as Trp53bp1) are immunodeficient owing to a complete loss of immunoglobulin class-switch recombination1,2, and reduced fidelity of long-range V(D)J rec...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0362-1
更新日期：2018-08-01 00:00:00
abstract：:For more than three-quarters of a century it has been assumed that basal metabolic rate increases as body mass raised to some power p. However, there is no broad consensus regarding the value of p: whereas many studies have asserted that p is 3/4 (refs 1-4; 'Kleiber's law'), some have argued that it is 2/3 (refs 5-7),...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08920
更新日期：2010-04-01 00:00:00
