Abstract:
:Growth factors are important regulators during organ development. For many vertebrates (but not humans) it is known how they contribute to the formation and expansion of PDX1-positive cells during pancreas organogenesis. Here, the effects of the fibroblast growth factors FGF2, FGF7, FGF10, and epidermal growth factor (EGF) on pancreas development in humans were assessed by using human pluripotent stem cells (hPSCs). During this, FGF2 was identified as a potent anti-pancreatic factor whereas FGF7, FGF10, and EGF increased the cell mass while retaining PDX1-positivity. FGF2 increased the expression of the anti-pancreatic factor sonic hedgehog (SHH) while suppressing PDX1 in a dose-dependent manner. Differentiating cells secreted SHH to the medium and we interrogated the cells' secretome during differentiation to globally examine the composition of secreted signaling factors. Members of the TGF-beta-, Wnt-, and FGF-pathways were detected. FGF17 showed a suppressive anti-pancreatic effect comparable to FGF2. By inhibition of specific branches of FGF-receptor signaling, we allocated the SHH-induction by FGF2 to MEK/ERK-signaling and the anti-pancreatic effect of FGF2 to the receptor variant FGFR1c or 3c. Altogether, we report findings on the paracrine activity of differentiating hPSCs during generation of pancreatic progenitors. These observations suggest a different role for FGF2 in humans compared to animal models of pancreas organogenesis.
journal_name
Cellsjournal_title
Cellsauthors
Dettmer R,Cirksena K,Münchhoff J,Kresse J,Diekmann U,Niwolik I,Buettner FFR,Naujok Odoi
10.3390/cells9091927subject
Has Abstractpub_date
2020-08-20 00:00:00issue
9issn
2073-4409pii
cells9091927journal_volume
9pub_type
杂志文章相关文献
Cells文献大全abstract::Stromal immune cells constitute the tumor microenvironment. These immune cell subsets include myeloid cells, the so-called tumor-associated myeloid cells (TAMCs), which are of two types: tumor-associated macrophages (TAMs) and myeloid-derived suppressor cells (MDSCs). Breast tumors, particularly those in human epiderm...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9081785
更新日期:2020-07-27 00:00:00
abstract::Promyelocytic leukemia (PML) bodies are dynamic intracellular structures that recruit and release a variety of different proteins in response to stress, virus infection, DNA damage and cell cycle progression. While PML bodies primarily are regarded as nuclear compartments, they are forced to travel to the cytoplasm ea...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells8080893
更新日期:2019-08-14 00:00:00
abstract::Cyclic AMP promotes EPAC1 and EPAC2 activation through direct binding to a specific cyclic nucleotide-binding domain (CNBD) within each protein, leading to activation of Rap GTPases, which control multiple cell responses, including cell proliferation, adhesion, morphology, exocytosis, and gene expression. As a result,...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8111425
更新日期:2019-11-12 00:00:00
abstract::Defects in membrane repair contribute to the development of some muscular dystrophies, highlighting the importance to decipher the membrane repair mechanisms in human skeletal muscle. In murine myofibers, the formation of a cap subdomain composed notably by annexins (Anx) is critical for membrane repair. We applied me...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9071742
更新日期:2020-07-21 00:00:00
abstract::Obesity and its comorbidities like diabetes, hypertension and other cardiovascular disorders are the leading causes of death and disability worldwide. Metabolic diseases cause vascular dysfunction and loss of capillaries termed capillary rarefaction. Interestingly, obesity seems to affect capillary beds in an organ-sp...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9122683
更新日期:2020-12-14 00:00:00
abstract::Nuclear transport receptors of the karyopherin superfamily of proteins transport macromolecules from one compartment to the other and are critical for both cell physiology and pathophysiology. The nuclear transport machinery is tightly regulated and essential to a number of key cellular processes since the spatiotempo...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells7110221
更新日期:2018-11-21 00:00:00
abstract::Background: The evidence on high-dose ifosfamide (HD-IFO) use in patients with relapsed osteosarcoma is limited. We performed a retrospective study to analyze HD-IFO activity. Methods: Patients with osteosarcoma relapsed after standard treatment [methotrexate, doxorubicin, cisplatin +/- ifosfamide (MAP+/-I)] with meas...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9112389
更新日期:2020-10-31 00:00:00
abstract::Investigating adaptive potential and understanding the relative roles of selection and genetic drift in populations of endangered species are essential in conservation. Major histocompatibility complex (MHC) genes characterized by spectacular polymorphism and fitness association have become valuable adaptive markers. ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8040377
更新日期:2019-04-25 00:00:00
abstract::P-glycoprotein (P-gp) is an important determinant of multidrug resistance (MDR) because its overexpression is associated with increased efflux of various established chemotherapy drugs in many clinically resistant and refractory tumors. This leads to insufficient therapeutic targeting of tumor populations, representin...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8101286
更新日期:2019-10-21 00:00:00
abstract::The genomes of all organisms abound with various cis-regulatory elements, which control gene activity. Transcriptional enhancers are a key group of such elements in eukaryotes and are DNA regions that form physical contacts with gene promoters and precisely orchestrate gene expression programs. Here, we follow gradual...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9071675
更新日期:2020-07-12 00:00:00
abstract::The nucleolus is a prominent, membraneless compartment found within the nucleus of eukaryotic cells. It forms around ribosomal RNA (rRNA) genes, where it coordinates the transcription, processing, and packaging of rRNA to produce ribosomal subunits. Recent efforts to characterize the biophysical properties of the nucl...
journal_title:Cells
pub_type: 社论
doi:10.3390/cells9030526
更新日期:2020-02-25 00:00:00
abstract::Hepatocellular carcinoma (HCC) has emerged as a major cause of cancer-related death and is the most common type of liver cancer. Due to the current paucity of drugs for HCC therapy there is a pressing need to develop new therapeutic concepts. In recent years, the role of Serum Response Factor (SRF) and its coactivator...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9122540
更新日期:2020-11-25 00:00:00
abstract::Lipid exchange among biological membranes, lipoprotein particles, micelles, and liposomes is an important yet underrated phenomenon with repercussions throughout the life sciences. The premature loss of lipid molecules from liposomal formulations severely impacts therapeutic applications of the latter and thus limits ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9102213
更新日期:2020-09-29 00:00:00
abstract::Langerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studies of BG have histor...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9040974
更新日期:2020-04-15 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPARs), members of the nuclear hormone receptor family, attract wide attention as promising therapeutic targets for the treatment of multiple diseases, and their target selective ligands were also intensively developed for pharmacological agents such as the approved drugs f...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9020352
更新日期:2020-02-03 00:00:00
abstract::Transient Receptor Potential Vanilloid 1 (TRPV1) subunits form a polymodal cation channel responsive to capsaicin, heat, acidity and endogenous metabolites of polyunsaturated fatty acids. While originally reported to serve as a pain and heat detector in the peripheral nervous system, TRPV1 has been implicated in the m...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells3030914
更新日期:2014-09-12 00:00:00
abstract::Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the diffe...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells6030018
更新日期:2017-07-03 00:00:00
abstract::The mechanism of intercellular transmission of pathological agents in neurodegenerative diseases has received much recent attention. Huntington's disease (HD) is caused by a monogenic mutation in the gene encoding Huntingtin (HTT). Mutant HTT (mHTT) harbors a CAG repeat extension which encodes an abnormally long polyg...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells7060059
更新日期:2018-06-14 00:00:00
abstract::Embryoid bodies (EBs) resemble self-organizing aggregates of pluripotent stem cells that recapitulate some aspects of early embryogenesis. Within few days, the cells undergo a transition from rather homogeneous epithelial-like pluripotent stem cell colonies into a three-dimensional organization of various cell types w...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9102270
更新日期:2020-10-11 00:00:00
abstract::Stefin B (cystatin B) is an intracellular inhibitor of cysteine cathepsins and mutations in the stefin B gene, resulting in the development of Unverricht-Lundborg disease, which is a form of myoclonic epilepsy. It was suggested that a key mechanism behind stefin B-mediated disease progression was impaired redox homeos...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8121476
更新日期:2019-11-21 00:00:00
abstract::During embryonic development, the heart undergoes complex morphogenesis from a liner tube into the four chambers consisting of ventricles, atria and valves. At the same time, the cardiomyocytes compact into a dense, aligned, and highly vascularized myocardium. The extracellular matrix (ECM) is known to play an importa...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9020285
更新日期:2020-01-24 00:00:00
abstract::Nile tilapia is the third most cultivated fish worldwide and a novel model species for evolutionary studies. Aiming to improve productivity and contribute to the selection of traits of economic impact, biotechnological approaches have been intensively applied to species enhancement. In this sense, recent studies have ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9081752
更新日期:2020-07-22 00:00:00
abstract::The discovery of the isocitrate dehydrogenase (IDH) mutation in glioma led to a paradigm shift on how we see glioma biology. Difficulties in cultivating IDH mutant glioma stem cells (IDHmut GSCs) resulted in a paucity of preclinical models in IDHmut glioma, limiting the discovery of new effective chemotherapeutic agen...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9061389
更新日期:2020-06-03 00:00:00
abstract::Glioblastoma (GBM) is one of the most intransigent and aggressive brain tumors, and its treatment is extremely challenging and ineffective. To improve patients' expectancy and quality of life, new therapeutic approaches were investigated. Melatonin is an endogenous indoleamine with an incredible variety of properties....
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9030599
更新日期:2020-03-03 00:00:00
abstract::Circular RNAs (circRNAs) are genome transcripts that are produced from back-splicing of specific regions of pre-mRNA. These single-stranded RNA molecules are widely expressed across diverse phyla and many of them are stable and evolutionary conserved between species. Growing evidence suggests that many circRNAs functi...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9061473
更新日期:2020-06-16 00:00:00
abstract::Cancer frequency and prevalence have been increasing in the past decades, with devastating impacts on patients and their families. Despite the great advances in targeted approaches, there is still a lack of methods to predict individual patient responses, and therefore treatments are tailored according to average resp...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9020293
更新日期:2020-01-25 00:00:00
abstract::Type 1 diabetes (T1D) is an autoimmune condition where the body's immune cells destroy their insulin-producing pancreatic beta cells leading to dysregulated glycaemia. Individuals with T1D control their blood glucose through exogenous insulin replacement therapy, often using multiple daily injections or pumps. However...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9112403
更新日期:2020-11-03 00:00:00
abstract::One third of all human proteins are either transmembrane or soluble secretory proteins that first target the endoplasmic reticulum (ER). These proteins subsequently leave the ER and enter the Golgi apparatus via ER-Golgi intermediate vesicular structures. Live-cell imaging of cargos fused to fluorescent proteins (FPs)...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9102311
更新日期:2020-10-17 00:00:00
abstract::Mutations in tubulin genes are responsible for a large spectrum of brain malformations secondary to abnormal neuronal migration, organization, differentiation and axon guidance and maintenance. Motor impairment, intellectual disability and epilepsy are the main clinical symptoms. In the present study 15 patients from ...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells8070669
更新日期:2019-07-02 00:00:00
abstract::Patient-derived tumor organoids (PDOs) represent a promising preclinical cancer model that better replicates disease, compared with traditional cell culture models. We have established PDOs from various human tumors to accurately and efficiently recapitulate the tissue architecture and function. Molecular targeted the...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8050481
更新日期:2019-05-20 00:00:00