Abstract：
:Defects in membrane repair contribute to the development of some muscular dystrophies, highlighting the importance to decipher the membrane repair mechanisms in human skeletal muscle. In murine myofibers, the formation of a cap subdomain composed notably by annexins (Anx) is critical for membrane repair. We applied membrane damage by laser ablation to human skeletal muscle cells and assessed the behavior of annexin-A6 (AnxA6) tagged with GFP by correlative light and electron microscopy (CLEM). We show that AnxA6 was recruited to the site of membrane injury within a few seconds after membrane injury. In addition, we show that the deficiency in AnxA6 compromises human sarcolemma repair, demonstrating the crucial role played by AnxA6 in this process. An AnxA6-containing cap-subdomain was formed in damaged human myotubes in about one minute. Through transmission electron microscopy (TEM), we observed that extension of the sarcolemma occurred during membrane resealing, which participated in forming a dense lipid structure in order to plug the hole. By properties of membrane folding and curvature, AnxA6 helped in the formation of this tight structure. The compaction of intracellular membranes-which are used for membrane resealing and engulfed in extensions of the sarcolemma-may also facilitate elimination of the excess of lipid and protein material once cell membrane has been repaired. These data reinforce the role played by AnxA6 and the cap subdomain in membrane repair of skeletal muscle cells.
journal_name
Cellsjournal_title
Cellsauthors
Croissant C,Gounou C,Bouvet F,Tan S,Bouter Adoi
10.3390/cells9071742subject
Has Abstractpub_date
2020-07-21 00:00:00issue
7issn
2073-4409pii
cells9071742journal_volume
9pub_type
杂志文章相关文献
Cells文献大全abstract：:HDGF-related protein 2 (HRP-2) is a member of the Hepatoma-Derived Growth Factor-related protein family that harbors the structured PWWP and Integrase Binding Domain, known to associate with methylated histone tails or cellular and viral proteins, respectively. Interestingly, HRP-2 is a paralog of Lens Epithelium Deri...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells10010192
更新日期：2021-01-19 00:00:00
abstract：:Stem cell therapy represents a promising approach in the treatment of several neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). The beneficial effect of stem cells is exerted by paracrine mediators, as exosomes, suggesting a possible potential use of these extracellular vesicles as non-cell b...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8091087
更新日期：2019-09-14 00:00:00
abstract：:Retinal neurons, particularly retinal ganglion cells (RGCs), are susceptible to the degenerative damage caused by different inherited conditions and environmental insults, leading to irreversible vision loss and, ultimately, blindness. Numerous strategies are being tested in different models of degeneration to restore...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9081759
更新日期：2020-07-22 00:00:00
abstract：:As a key hub of malignant properties, the cancer microenvironment plays a crucial role intimately connected to tumor properties. Accumulating evidence supports that the lysophospholipid sphingosine-1-phosphate acts as a key signal in the cancer extracellular milieu. In this review, we have a particular focus on gliobl...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9020337
更新日期：2020-02-01 00:00:00
abstract：:Tumors lack a well-regulated vascular supply of O2 and often fail to balance O2 supply and demand. Net O2 tension within many tumors may not only depend on O2 delivery but also depend strongly on O2 demand. Thus, tumor O2 consumption rates may influence tumor hypoxia up to true anoxia. Recent reports have shown that m...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8121487
更新日期：2019-11-21 00:00:00
abstract：:CCN1 and CCN2 are members of the CCN family and play essential roles in the regulation of multiple female reproductive functions, including ovulation. Cyclooxygenase-2 (COX2) is a critical mediator of ovulation and can be induced by sphingosine-1-phosphate (S1P) through the S1P1/3-mediated Yes-associated protein (YAP)...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8111445
更新日期：2019-11-15 00:00:00
abstract：:The crosstalk between human gut microbiota and intestinal wall is essential for the organ's homeostasis and immune tolerance. The gut microbiota plays a role in healthy and pathological conditions mediated by inflammatory processes or by the gut-brain axes, both involving a possible role for S100B protein as a diffusi...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071697
更新日期：2020-07-15 00:00:00
abstract：:In liver transplantation (LT), organ shortage has led to the use of steatotic and non-steatotic grafts from donors after cardiocirculatory death (DCD). However, these grafts, especially those with steatosis, exhibit poor post-operative outcomes. To address this problem, we investigated the roles of gut-derived glucago...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8121599
更新日期：2019-12-09 00:00:00
abstract：:Lyme Borreliosis is an infectious disease caused by the spirochete Borrelia burgdorferi that is transmitted through the bite of infected ticks. Both B cell-mediated humoral immunity and T cell immunity develop during natural Borrelia infection. However, compared with humoral immunity, the T cell response to Borrelia i...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells2030607
更新日期：2013-09-13 00:00:00
abstract：:Angiogenesis is defined as the formation of new blood vessels and is a key phenomenon manifested in a host of cancers during which tyrosine kinases play a crucial role. Vascular endothelial growth factor receptor-2 (VEGFR-2) is pivotal in cancer angiogenesis, which warrants the urgency of discovering new anti-angiogen...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8030269
更新日期：2019-03-21 00:00:00
abstract：:Tumor-infiltrating lymphocytes are known to be critical in controlling tumor progression. While the role of T lymphocytes has been extensively studied, the function of B cells in this context is still ill-defined. In this review, we propose to explore the role of B cells in tumor immunity. First of all we define their...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8050449
更新日期：2019-05-13 00:00:00
abstract：:In this study, the mechanism by which GSK-3β regulates protein synthesis and lipid deposition was investigated in zebrafish (Danio rerio). The vector of pEGFP-N1-GSK-3β was constructed and injected into the muscle of zebrafish. It was found that the mRNA and protein expression of tuberous sclerosis complex 2 (TSC2) wa...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8030205
更新日期：2019-02-28 00:00:00
abstract：:Ulcerative colitis is a chronic inflammatory disease confined to the colon. Although the etiopathogenesis remains unknown, small bowel dysfunctions like histological and permeability alterations have been described in ulcerative colitis. We evaluated the molecular gene signature in the non-inflamed terminal ileum of 3...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9081793
更新日期：2020-07-28 00:00:00
abstract：:Breast cancer is a heterogeneous disease that can be subdivided into unique molecular subtypes based on protein expression of the Estrogen Receptor, Progesterone Receptor, and/or the Human Epidermal Growth Factor Receptor 2. Therapeutic approaches are designed to inhibit these overexpressed receptors either by endocri...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9102204
更新日期：2020-09-29 00:00:00
abstract：:Most protein-encoding genes in eukaryotes contain introns, which are interwoven with exons. Introns need to be removed from initial transcripts in order to generate the final messenger RNA (mRNA), which can be translated into an amino acid sequence. Precise excision of introns by the spliceosome requires conserved din...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9020458
更新日期：2020-02-18 00:00:00
abstract：:Blood vessel formation or angiogenesis is a key process for successful tooth regeneration. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) possess paracrine proangiogenic properties, which are, at least partially, induced by their extracellular vesicles (EVs). However, the isolation of BM-MSCs is associated wi...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9020312
更新日期：2020-01-28 00:00:00
abstract：:Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a component of the extracellular environment, where it mediates diverse processes including matrix regulation/turnover, inflammation and angiogenesis. Rare TIMP-3 risk alleles and mutations are directly linked with retinopathies such as age-related macular degenerati...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9010039
更新日期：2019-12-21 00:00:00
abstract：:Nuclear mRNA export plays an important role in gene expression. We describe the mechanisms of mRNA export including the importance of mRNP assembly, docking with the nuclear basket of the nuclear pore complex (NPC), transit through the central channel of the NPC and cytoplasmic release. We describe multiple mechanisms...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells4030452
更新日期：2015-08-28 00:00:00
abstract：:The current coronavirus disease-2019 (COVID-19) pandemic is due to the novel coronavirus SARS-CoV-2. The scientific community has mounted a strong response by accelerating research and innovation, and has quickly set the foundation for understanding the molecular determinants of the disease for the development of targ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9051267
更新日期：2020-05-20 00:00:00
abstract：:Sepsis is a leading cause of death in intensive care units, and cardiac dysfunction is an identified serious component of the multi-organ failure associated with this critical condition. This review summarized the current discoveries and hypothesizes of how autophagy changes in the heart during sepsis and the underlyi...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8020141
更新日期：2019-02-10 00:00:00
abstract：:Previously, we used a lentiviral vector to deliver furin-cleavable human insulin (INS-FUR) to the livers in several animal models of diabetes using intervallic infusion in full flow occlusion (FFO), with resultant reversal of diabetes, restoration of glucose tolerance and pancreatic transdifferentiation (PT), due to t...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9102227
更新日期：2020-10-02 00:00:00
abstract：:Hspb8 is a member of the small heat shock protein (sHSP) family. Its expression is known to be upregulated under heat shock. This protein interacts with different partners and can, therefore, be involved in various processes relevant to tissue integrity and functioning. In humans, mutations in the gene encoding Hspb8 ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9061562
更新日期：2020-06-26 00:00:00
abstract：:We developed tumor-targeting Salmonella typhimurium (S. typhimurium) A1-R, a facultative anaerobe that is an auxotroph of leucine and arginine. The tumor-targeting efficacy of S. typhimurium A1-R was demonstrated in vivo and vitro using several malignant cell lines including melanoma, sarcoma, glioma, breast, pancreat...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8060599
更新日期：2019-06-16 00:00:00
abstract：:(1) Background: Refractory acute graft-versus-host disease (R-aGvHD) remains a leading cause of death after allogeneic stem cell transplantation. Survival rates of 15% after four years are currently achieved; deaths are only in part due to aGvHD itself, but mostly due to adverse effects of R-aGvHD treatment with immun...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8121577
更新日期：2019-12-05 00:00:00
abstract：:The human gut relies on several cellular and molecular mechanisms to allow for an intact and dynamical intestinal barrier. Normally, only small amounts of luminal content pass the mucosa, however, if the control is broken it can lead to enhanced passage, which might damage the mucosa, leading to pathological condition...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8020193
更新日期：2019-02-22 00:00:00
abstract：:Cancer is a complex expression of an altered state of cellular differentiation associated with severe clinical repercussions. The effort to characterize this pathological entity to understand its underlying mechanisms and visualize potential therapeutic strategies has been constant. In this context, some cellular (enh...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9071612
更新日期：2020-07-03 00:00:00
abstract：:Commonly, circulating tumor cells (CTCs) are described as source of metastasis in cancer patients. However, in this process cancer cells of the primary tumor site need to survive the physical and biological challenges in the blood stream before leaving the circulation to become the seed of a new metastatic site in dis...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8070714
更新日期：2019-07-13 00:00:00
abstract：:The primary cilium is an organelle involved in cellular signalling. Mutations affecting proteins involved in cilia assembly or function result in diseases known as ciliopathies, which cause a wide variety of phenotypes across multiple tissues. These mutations disrupt various cellular processes, including regulation of...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9020278
更新日期：2020-01-23 00:00:00
abstract：:In order to provide better therapy we strive to increase our knowledge of how the immune system behaves and communicates in common pediatric immunological diseases, such as type 1 diabetes, allergic and celiac diseases. However, when dealing with pediatric diseases, where study subjects are almost exclusively children...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells1020141
更新日期：2012-05-29 00:00:00
abstract：:Cancer cells perform a metabolic rewiring to sustain an increased growth rate and compensate for the redox stress caused by augmented energy metabolism. The metabolic changes are not the same in all cancers. Some features, however, are considered hallmarks of this disease. As an example, all cancer cells rewire the am...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9092028
更新日期：2020-09-03 00:00:00
