Abstract：
:Most protein-encoding genes in eukaryotes contain introns, which are interwoven with exons. Introns need to be removed from initial transcripts in order to generate the final messenger RNA (mRNA), which can be translated into an amino acid sequence. Precise excision of introns by the spliceosome requires conserved dinucleotides, which mark the splice sites. However, there are variations of the highly conserved combination of GT at the 5' end and AG at the 3' end of an intron in the genome. GC-AG and AT-AC are two major non-canonical splice site combinations, which have been known for years. Recently, various minor non-canonical splice site combinations were detected with numerous dinucleotide permutations. Here, we expand systematic investigations of non-canonical splice site combinations in plants across eukaryotes by analyzing fungal and animal genome sequences. Comparisons of splice site combinations between these three kingdoms revealed several differences, such as an apparently increased CT-AC frequency in fungal genome sequences. Canonical GT-AG splice site combinations in antisense transcripts are a likely explanation for this observation, thus indicating annotation errors. In addition, high numbers of GA-AG splice site combinations were observed in Eurytemoraaffinis and Oikopleuradioica. A variant in one U1 small nuclear RNA (snRNA) isoform might allow the recognition of GA as a 5' splice site. In depth investigation of splice site usage based on RNA-Seq read mappings indicates a generally higher flexibility of the 3' splice site compared to the 5' splice site across animals, fungi, and plants.
journal_name
Cellsjournal_title
Cellsauthors
Frey K,Pucker Bdoi
10.3390/cells9020458subject
Has Abstractpub_date
2020-02-18 00:00:00issue
2issn
2073-4409pii
cells9020458journal_volume
9pub_type
杂志文章相关文献
Cells文献大全abstract：:Gene expression is often altered by epigenetic modifications that can significantly influence the growth ability and progression of cancers. SAHA (Suberoylanilide hydroxamic acid, also known as Vorinostat), a well-known Histone deacetylase (HDAC) inhibitor, can stop cancer growth and metastatic processes through epige...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8010008
更新日期：2018-12-22 00:00:00
abstract：:There is a need to disentangle the etiological puzzle of age-related neurodegenerative diseases, whose clinical phenotypes arise from known, and as yet unknown, pathways that can act distinctly or in concert. Enhanced sub-phenotyping and the identification of in vivo biomarker-driven signature profiles could improve t...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9122581
更新日期：2020-12-02 00:00:00
abstract：:Leptin is one of the main adipokines secreted in breast tissue. Leptin promotes epithelial-mesenchymal transition (EMT), cell migration and invasion in epithelial breast cells, leading to tumor progression. Although, the molecular mechanisms that underlie these events are not fully understood, the activation of differ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8101133
更新日期：2019-09-24 00:00:00
abstract：:EGFR and some of the cognate ligands extensively traffic in extracellular vesicles (EVs) from different biogenesis pathways. EGFR belongs to a family of four homologous tyrosine kinase receptors (TKRs). This family are one of the major drivers of cancer and is involved in several of the most frequent malignancies such...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9112505
更新日期：2020-11-19 00:00:00
abstract：:Ulcerative colitis is a chronic inflammatory disease confined to the colon. Although the etiopathogenesis remains unknown, small bowel dysfunctions like histological and permeability alterations have been described in ulcerative colitis. We evaluated the molecular gene signature in the non-inflamed terminal ileum of 3...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9081793
更新日期：2020-07-28 00:00:00
abstract：:Cancer cells rely on aberrant transcription for growth and survival. Cyclin-dependent kinases (CDKs) play critical roles in regulating gene transcription by modulating the activity of RNA polymerase II (RNAPII). THZ1, a selective covalent inhibitor of CDK7, has antitumor effects in several human cancers. In this study...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8111469
更新日期：2019-11-19 00:00:00
abstract：:Aging is associated with metabolic changes and low-grade inflammation in several organs, which may be due to NLRP3 inflammasome activation. Methods: Here, we asked whether age-related liver changes such as lipid metabolism and fibrosis are reduced in aged mice lacking the NLRP3 inflammasome. We report reduced protein ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9102148
更新日期：2020-09-23 00:00:00
abstract：:Langerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studies of BG have histor...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9040974
更新日期：2020-04-15 00:00:00
abstract：:Cell plasticity, defined as the ability to undergo phenotypical transformation in a reversible manner, is a physiological process that also exerts important roles in disease progression. Two forms of cellular plasticity are epithelial-mesenchymal transition (EMT) and its inverse process, mesenchymal-epithelial transit...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8121644
更新日期：2019-12-15 00:00:00
abstract：:Lysosomal function has a central role in maintaining neuronal homeostasis, and, accordingly, lysosomal dysfunction has been linked to neurodegeneration and particularly to Parkinson's disease (PD). Lysosomes are the converging step where the substrates delivered by autophagy and endocytosis are degraded in order to re...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9112399
更新日期：2020-11-02 00:00:00
abstract：:Embryoid bodies (EBs) resemble self-organizing aggregates of pluripotent stem cells that recapitulate some aspects of early embryogenesis. Within few days, the cells undergo a transition from rather homogeneous epithelial-like pluripotent stem cell colonies into a three-dimensional organization of various cell types w...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9102270
更新日期：2020-10-11 00:00:00
abstract：:Vinyl chloride (VC) is a noninfective occupational risk factor. It is found in industrial chemicals, volatile organic compounds, cigarette smoke ingredients, etc. It is a kind of toxic gas that causes many diseases. VC exposure causes an increased risk of liver fibrosis and can result in angiosarcoma of the liver. Pre...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8060601
更新日期：2019-06-17 00:00:00
abstract：:The NF-κB transcription factor pathway is a crucial regulator of inflammation and immune responses. Additionally, aberrant NF-κB signaling has been identified in many types of cancer. Downstream of key oncogenic pathways, such as RAS, BCR-ABL, and Her2, NF-κB regulates transcription of target genes that promote cell s...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells5020016
更新日期：2016-04-06 00:00:00
abstract：:Identification of patients with activation of the immune system which indicates the presence of infection is essential, especially in the times of the global coronavirus 2019 (COVID-19) pandemic. The aim of the present study was to evaluate the reactive lymphocytes (RE-LYMP) parameter in COVID-19 and to correlate it w...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells10010082
更新日期：2021-01-06 00:00:00
abstract：:Glioblastoma multiforme is one of the most malignant types of cancer. This is mainly due to a cell subpopulation with an extremely aggressive potential, called glioblastoma stem-like cells (GSCs). These cells produce high levels of extracellular adenosine which has been associated with increased chemoresistance, migra...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9081914
更新日期：2020-08-18 00:00:00
abstract：:Actin and non-muscle myosins have long been known to play important roles in growth cone steering and neurite outgrowth. More recently, novel functions for non-muscle myosin have been described in axons and dendritic spines. Consequently, possible roles of actomyosin contraction in organizing and maintaining structura...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9092006
更新日期：2020-09-01 00:00:00
abstract：:Alcohol use disorder is associated with a wide array of hepatic pathologies ranging from steatosis to alcoholic-related cirrhosis (AC), alcoholic hepatitis (AH), or hepatocellular carcinoma (HCC). Biomarkers are categorized into two main categories: biomarkers associated with alcohol consumption and biomarkers of alco...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9030524
更新日期：2020-02-25 00:00:00
abstract：:Xanthohumol (XN), a prenylated chalcone from hops, has been reported to exhibit a variety of health-beneficial effects. However, poor bioavailability may limit its application in the prevention and therapy of diseases. The objective of this study was to determine whether a micellar solubilization of xanthohumol could ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8040359
更新日期：2019-04-17 00:00:00
abstract：:Extracellular matrix (ECM) remodeling is required for many physiological and pathological processes. Metalloproteinases (MMPs) are endopeptidases which are able to degrade different components of the ECM and nucleus matrix and to cleave numerous non-ECM proteins. Among pathological processes, MMPs are involved in adip...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8020158
更新日期：2019-02-14 00:00:00
abstract：:Tissue-resident macrophages play critical roles in controlling homeostasis, tissue repair, and immunity. Inflammatory macrophages can sustain tissue damage and promote the development of fibrosis during infections and sterile tissue injury. The NLRP3 inflammasome and its effector cytokine IL-1β have been identified as...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9010079
更新日期：2019-12-28 00:00:00
abstract：:In the fruit fly, Drosophila melanogaster, the larval somatic muscles or the adult thoracic flight and leg muscles are the major voluntary locomotory organs. They share several developmental and structural similarities with vertebrate skeletal muscles. To ensure appropriate activity levels for their functions such as ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9061543
更新日期：2020-06-25 00:00:00
abstract：:More than 50 years after the first description of Bronchopulmonary dysplasia (BPD) by Northway, this chronic lung disease affecting many preterm infants is still poorly understood. Additonally, approximately 40% of preterm infants suffering from severe BPD also suffer from Bronchopulmonary dysplasia-associated pulmona...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9081875
更新日期：2020-08-11 00:00:00
abstract：:Synchronous communication between the developing embryo and the receptive endometrium is crucial for embryo implantation. Thus, uterine receptivity evaluation is vital in managing recurrent implantation failure (RIF). The potential roles of small extracellular vesicle (sEV) miRNAs in pregnancy have been widely studied...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9030645
更新日期：2020-03-06 00:00:00
abstract：:Integrin activation is essential for creating functional transmembrane receptors capable of inducing downstream cellular effects such as cell migration, cell spreading, neurite outgrowth and axon regeneration. Integrins are bidirectional signalling molecules that mediate their effects by 'inside-out' and 'outside-in' ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells7030020
更新日期：2018-03-10 00:00:00
abstract：:Pharmaco-therapeutic strategies of atrial fibrillation (AF) are moderately effective and do not prevent AF onset and progression. Therefore, there is an urgent need to develop novel therapies. Previous studies revealed heat shock protein (HSP)-inducing compounds to mitigate AF onset and progression. Such an HSP induci...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071729
更新日期：2020-07-20 00:00:00
abstract：:Evaluation of myelin by magnetic resonance imaging (MRI) is a difficult challenge, but holds promise in demyelinating diseases, such as multiple sclerosis (MS). Although multiple techniques have been developed, no gold standard has been established. This study aims to evaluate the correlation between synthetic MRI mye...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9020393
更新日期：2020-02-08 00:00:00
abstract：:Nuclear lamins (NLs) are essential components of the animal cell nucleus involved in the regulation of a plethora of molecular and cellular processes. These include the nuclear envelope assembly and stability, mechanotransduction and chromatin organization, transcription, DNA replication, damage repair, and genomic in...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9061340
更新日期：2020-05-27 00:00:00
abstract：:Although G protein-coupled receptor kinases (GRKs) have long been known to regulate G protein-coupled receptor (GPCR) desensitization, their more recently characterized functions as scaffolds and signalling adapters underscore that this small family of proteins governs a larger array of physiological functions than or...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells10010075
更新日期：2021-01-05 00:00:00
abstract：:The 70 kDa heat shock protein (HSP70) is a stress-inducible protein that has been shown to protect the brain from various nervous system injuries. It allows cells to withstand potentially lethal insults through its chaperone functions. Its chaperone properties can assist in protein folding and prevent protein aggregat...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9092020
更新日期：2020-09-02 00:00:00
abstract：:In response to inflammatory stimuli, immune cells reconfigure their metabolism and bioenergetics to generate energy and substrates for cell survival and to launch immune effector functions. As a critical component of the innate immune system, the nucleotide-binding and oligomerization domain, leucine-rich repeat, and ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9081808
更新日期：2020-07-30 00:00:00
