Abstract：
:Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba Dictyostelium discoideum as a relevant model to study autophagy. Several methods have been developed based on the tracking and observation of autophagosomes by microscopy, analysis of changes in expression of autophagy genes and proteins, and examination of the autophagic flux with various techniques. In this review, we discuss the pros and cons of the currently available techniques to assess autophagy in this organism.
journal_name
Cellsjournal_title
Cellsauthors
Domínguez-Martín E,Cardenal-Muñoz E,King JS,Soldati T,Coria R,Escalante Rdoi
10.3390/cells6030018subject
Has Abstractpub_date
2017-07-03 00:00:00issue
3issn
2073-4409pii
cells6030018journal_volume
6pub_type
杂志文章,评审相关文献
Cells文献大全abstract：:Recent advances in psychiatric genetics have led to the discovery of dozens of genomic loci associated with schizophrenia. However, a gap exists between the detection of genetic associations and understanding the underlying molecular mechanisms. This review describes the basic approaches used in the so-called post-GWA...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9010246
更新日期：2020-01-18 00:00:00
abstract：:As sessile organisms, plants must adjust their growth to withstand several environmental conditions. The root is a crucial organ for plant survival as it is responsible for water and nutrient acquisition from the soil and has high phenotypic plasticity in response to a lack or excess of them. How plants sense and tran...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9122576
更新日期：2020-12-01 00:00:00
abstract：:Network-based systems biology has become an important method for analyzing high-throughput gene expression data and gene function mining. Escherichia coli (E. coli) has long been a popular model organism for basic biological research. In this paper, weighted gene co-expression network analysis (WGCNA) algorithm was ap...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells7030019
更新日期：2018-03-08 00:00:00
abstract：:Antiphospholipid syndrome (APS) is a systemic autoimmune disease, characterized by thrombosis, obstetric complications and the presence of antiphospholipid antibodies (aPL), which drive endothelial injury and thrombophilia. Extracellular vesicles (EVs) have been implicated in endothelial and thrombotic pathologies. He...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9051211
更新日期：2020-05-13 00:00:00
abstract：:The NF-κB transcription factor pathway is a crucial regulator of inflammation and immune responses. Additionally, aberrant NF-κB signaling has been identified in many types of cancer. Downstream of key oncogenic pathways, such as RAS, BCR-ABL, and Her2, NF-κB regulates transcription of target genes that promote cell s...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells5020016
更新日期：2016-04-06 00:00:00
abstract：:Integrin activation is essential for creating functional transmembrane receptors capable of inducing downstream cellular effects such as cell migration, cell spreading, neurite outgrowth and axon regeneration. Integrins are bidirectional signalling molecules that mediate their effects by 'inside-out' and 'outside-in' ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells7030020
更新日期：2018-03-10 00:00:00
abstract：:Infectious endocarditis (IE) remains one of the deadliest heart diseases with a high death rate, generally following thrombo-embolic events. Today, therapy is based on surgery and antibiotic therapy. When thromboembolic complications in IE patients persist, this is often due to our lack of knowledge regarding the path...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9081899
更新日期：2020-08-13 00:00:00
abstract：:Multiple Sclerosis (MS) is a chronic demyelinating autoimmune disease primarily affecting young adults. Despite an unclear causal factor, symptoms and pathology arise from the infiltration of peripheral immune cells across the blood brain barrier. Accounting for the largest fraction of this infiltrate, macrophages are...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8060543
更新日期：2019-06-05 00:00:00
abstract：:The authors wish to make the following corrections to this paper [...]. ...
journal_title：Cells
pub_type： 已发布勘误
doi：10.3390/cells9102165
更新日期：2020-09-24 00:00:00
abstract：:The role of kinases in the regulation of cell cycle transitions is very well established, however, over the past decade, studies have identified the ever-growing importance of phosphatases in these processes. It is well-known that an intact or otherwise non-deformed nuclear envelope (NE) is essential for maintaining h...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8070687
更新日期：2019-07-07 00:00:00
abstract：:One of the crucial challenges in the clinical management of cancer is resistance to chemotherapeutics. Multidrug resistance (MDR) has been intensively studied, and one of the most prominent mechanisms underlying MDR is overexpression of adenosine triphosphate (ATP)-binding cassette (ABC) transporters. Despite research...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells7080107
更新日期：2018-08-14 00:00:00
abstract：:The replication independent (RI) histone H2A.Z is one of the more extensively studied variant members of the core histone H2A family, which consists of many replication dependent (RD) members. The protein has been shown to be indispensable for survival, and involved in multiple roles from DNA damage to chromosome segr...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9051167
更新日期：2020-05-08 00:00:00
abstract：:Receptor tyrosine kinases (RTKs) are membrane-based sensors that enable rapid communication between cells and their environment. Evidence is now emerging that interdependent regulatory mechanisms, such as membrane trafficking, ubiquitination, proteolysis and gene expression, have substantial effects on RTK signal tran...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells7030022
更新日期：2018-03-15 00:00:00
abstract：:Many cells rearrange proteins and other components into spatially distinct domains in a process called polarization. This asymmetric patterning is required for a number of biological processes including asymmetric division, cell migration, and embryonic development. Proteins involved in polarization are highly conserv...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9092036
更新日期：2020-09-05 00:00:00
abstract：:Differentiation therapy is an alternative strategy used to induce the differentiation of blast cells toward mature cells and to inhibit tumor cell proliferation for cancer treatment. Nobiletin (NOB), a polymethoxyflavone phytochemical, is present abundantly in citrus peels and has been reported to possess anti-cancer ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9040877
更新日期：2020-04-03 00:00:00
abstract：:Plasma exosomal microRNAs (miRNAs) are considered as valid circulating biomarkers for cancer diagnosis and prognosis. Quantitative real-time polymerase chain reaction (qRT-PCR), the most commonly used technique to assess circulating miRNA levels, requires a normalization step involving uniformly expressed endogenous m...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells10010101
更新日期：2021-01-08 00:00:00
abstract：:Cyclic AMP promotes EPAC1 and EPAC2 activation through direct binding to a specific cyclic nucleotide-binding domain (CNBD) within each protein, leading to activation of Rap GTPases, which control multiple cell responses, including cell proliferation, adhesion, morphology, exocytosis, and gene expression. As a result,...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8111425
更新日期：2019-11-12 00:00:00
abstract：:NFATc1, which is ubiquitous in many cell types, is the master regulator of osteoclastogenesis. However, the molecular mechanisms by which NFATc1 drives its transcriptional program to produce osteoclasts from macrophages (M) remains poorly understood. We performed quantitative PCR (QPCR) arrays and bioinformatic analys...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8020131
更新日期：2019-02-07 00:00:00
abstract：:Depression is a very common psychiatric disorder affecting approximately 300 million people worldwide with the prevalence being twice as high in women as in men. Despite intense research efforts in recent decades, the neurobiological basis underlying depression remains incompletely understood. However, the exposure to...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8091018
更新日期：2019-09-01 00:00:00
abstract：:Reactive oxygen species (ROS) are produced continuously throughout the cell as products of various redox reactions. Yet these products function as important signal messengers, acting through oxidation of specific target factors. Whilst excess ROS production has the potential to induce oxidative stress, physiological r...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells10020233
更新日期：2021-01-25 00:00:00
abstract：:Mutations in tubulin genes are responsible for a large spectrum of brain malformations secondary to abnormal neuronal migration, organization, differentiation and axon guidance and maintenance. Motor impairment, intellectual disability and epilepsy are the main clinical symptoms. In the present study 15 patients from ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8070669
更新日期：2019-07-02 00:00:00
abstract：:The genomes of all organisms abound with various cis-regulatory elements, which control gene activity. Transcriptional enhancers are a key group of such elements in eukaryotes and are DNA regions that form physical contacts with gene promoters and precisely orchestrate gene expression programs. Here, we follow gradual...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9071675
更新日期：2020-07-12 00:00:00
abstract：:Chronic inflammation and abnormalities in Toll-like receptor (TLR) signaling pathways are associated with major depressive disorder (MDD). Our previous work reported that impaired negative regulators for the TLR pathways are associated with MDD. This study aimed to assess the association between the severity of depres...
journal_title：Cells
pub_type： 临床试验,杂志文章
doi：10.3390/cells8070647
更新日期：2019-06-27 00:00:00
abstract：:Human Toll-like receptor (TLR) signaling plays a vital role in intestinal inflammation by activating the NF-B pathway. By querying GENT2 datasets, we identified the gene expression level of TLR2 and TLR4 as being substantially increased in colorectal cancer. Introduction of shRNAs for TLR4 but not TLR2 dramatically re...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9020397
更新日期：2020-02-09 00:00:00
abstract：:The failure of drug efficacy in clinical trials remains a big issue in cancer research. This is largely due to the limitations of two-dimensional (2D) cell cultures, the most used tool in drug screening. Nowadays, three-dimensional (3D) cultures, including spheroids, are acknowledged to be a better model of the in viv...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9030703
更新日期：2020-03-13 00:00:00
abstract：:The survival of most patients with advanced stage non-small cell lung cancer is prolonged by several months when they are treated with first- and next-generation inhibitors targeting ALK rearrangements, but resistance inevitably emerges. Some of the mechanisms of resistance are sensitive to novel ALK inhibitors but af...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells10010168
更新日期：2021-01-15 00:00:00
abstract：:One key issue in the post-genomic era is how to systematically describe the associations between small molecule transcripts or translations inside cells. With the rapid development of high-throughput "omics" technologies, the achieved ability to detect and characterize molecules with other molecule targets opens the p...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8080866
更新日期：2019-08-09 00:00:00
abstract：:: Brains that are affected by Alzheimer's disease (AD) are characterized by the overload of extracellular amyloid β (Aβ) peptides, but recent data from cellular and animal models propose that Aβ deposition is preceded by intraneuronal accumulation of the direct precursor of Aβ, C99. These studies indicate that C99 acc...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9051204
更新日期：2020-05-12 00:00:00
abstract：:Small GTPases regulate cytoskeletal dynamics, cell motility, and division under precise spatiotemporal control. Different small GTPases exhibit cross talks to exert feedback response or to act in concert during signal transduction. However, whether and how specific cytoskeletal components' feedback to upstream signali...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8101264
更新日期：2019-10-16 00:00:00
abstract：:Circular RNAs (circRNAs) are genome transcripts that are produced from back-splicing of specific regions of pre-mRNA. These single-stranded RNA molecules are widely expressed across diverse phyla and many of them are stable and evolutionary conserved between species. Growing evidence suggests that many circRNAs functi...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9061473
更新日期：2020-06-16 00:00:00
