A rapid pyrosequencing assay for the molecular detection of influenza viruses with reduced baloxavir susceptibility due to PA/I38X amino acid substitutions.


:Baloxavir marboxil is a novel endonuclease inhibitor licensed for treatment of otherwise healthy or high-risk individuals infected with influenza. Viruses with reduced baloxavir susceptibility due to amino acid substitutions at residue 38 of the PA have been detected in some individuals following treatment. Here, we describe a genotypic pyrosequencing method that can be used to rapidly screen circulating influenza A and B viruses for substitutions in the PA/I38 codon and to quantify mixed viral populations. This method is suitable for surveillance of baloxavir susceptibility and to analyse samples from hospitalised patients undergoing baloxavir treatment to aid in clinical decision making.


Koszalka P,Farrukee R,Mifsud E,Vijaykrishna D,Hurt AC




Has Abstract


2020-07-01 00:00:00












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    abstract:BACKGROUND:The 1918 influenza pandemic caused disproportionately high mortality among certain age groups. The mechanisms underlying these differences are not fully understood. OBJECTIVES:To explore the dynamics of the 1918 pandemic and to identify potential age-specific transmission patterns. METHODS:We examined 1915...

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    pub_type: 历史文章,杂志文章


    authors: Yang W,Petkova E,Shaman J

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  • Clinic accessibility and clinic-level predictors of the geographic variation in 2009 pandemic influenza vaccine coverage in Montreal, Canada.

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    pub_type: 杂志文章


    authors: Charland KM,de Montigny L,Brownstein JS,Buckeridge DL

    更新日期:2014-05-01 00:00:00

  • Detection of enterovirus 68 in serum from pediatric patients with pneumonia and their clinical outcomes.

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    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Imamura T,Suzuki A,Lupisan S,Kamigaki T,Okamoto M,Roy CN,Olveda R,Oshitani H

    更新日期:2014-01-01 00:00:00

  • 1918 pandemic morbidity: The first wave hits the poor, the second wave hits the rich.

    abstract:BACKGROUND:Whether morbidity from the 1918-19 influenza pandemic discriminated by socioeconomic status has remained a subject of debate for 100 years. In lack of data to study this issue, the recent literature has hypothesized that morbidity was "socially neutral." OBJECTIVES:To study the associations between influenz...

    journal_title:Influenza and other respiratory viruses

    pub_type: 历史文章,杂志文章


    authors: Mamelund SE

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    abstract:BACKGROUND:School-located influenza vaccination (SLV) programs have the potential to mass-vaccinate all enrolled children, but parental consent is required. OBJECTIVE:To examine parental attitudes and determine predictors of parental consent for vaccination of schoolchildren through SLV programs. PATIENTS/METHODS:Sur...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Cheung S,Wang HL,Mascola L,El Amin AN,Pannaraj PS

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  • The national inventory of core capabilities for pandemic influenza preparedness and response: an instrument for planning and evaluation.

    abstract:BACKGROUND:Reviews of the global response to the 2009 pandemic of influenza A/H1N1 affirmed the importance of assessment of preparedness and response capabilities. DESIGN:The U. S. Centers for Disease Control and Prevention (CDC) and partners developed the National Inventory of Core Capabilities for Pandemic Influenza...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: MacDonald G,Moen AC,St Louis ME

    更新日期:2014-03-01 00:00:00

  • Inter-seasonality of influenza in Australia.

    abstract:BACKGROUND:It appears inter-seasonal influenza notifications have been increasing in summer months in Australia. This study aims to determine changes in inter-seasonal influenza activity in Australia over time. METHODS:Routine influenza surveillance data and hospitalisations data were analysed to study the epidemiolog...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Moa AM,Adam DC,MacIntyre CR

    更新日期:2019-09-01 00:00:00

  • Medical masks vs N95 respirators for preventing COVID-19 in healthcare workers: A systematic review and meta-analysis of randomized trials.

    abstract:BACKGROUND:Respiratory protective devices are critical in protecting against infection in healthcare workers at high risk of novel 2019 coronavirus disease (COVID-19); however, recommendations are conflicting and epidemiological data on their relative effectiveness against COVID-19 are limited. PURPOSE:To compare medi...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章,meta分析


    authors: Bartoszko JJ,Farooqi MAM,Alhazzani W,Loeb M

    更新日期:2020-07-01 00:00:00

  • Unemployment and widespread influenza in America, 1999-2010.

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    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Cornwell B

    更新日期:2012-01-01 00:00:00

  • An evidence synthesis approach to estimating the incidence of seasonal influenza in the Netherlands.

    abstract:OBJECTIVES:To estimate, using Bayesian evidence synthesis, the age-group-specific annual incidence of symptomatic infection with seasonal influenza in the Netherlands over the period 2005-2007. METHODS:The Netherlands population and age group distribution for 2006 defined the base population. The number of influenza-l...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: McDonald SA,Presanis AM,De Angelis D,van der Hoek W,Hooiveld M,Donker G,Kretzschmar ME

    更新日期:2014-01-01 00:00:00

  • The efficacy of medical masks and respirators against respiratory infection in healthcare workers.

    abstract:OBJECTIVE:We aimed to examine the efficacy of medical masks and respirators in protecting against respiratory infections using pooled data from two homogenous randomised control clinical trials (RCTs). METHODS:The data collected on 3591 subjects in two similar RCTs conducted in Beijing, China, which examined the same ...

    journal_title:Influenza and other respiratory viruses

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: MacIntyre CR,Chughtai AA,Rahman B,Peng Y,Zhang Y,Seale H,Wang X,Wang Q

    更新日期:2017-11-01 00:00:00

  • Human bocavirus amongst an all-ages population hospitalised with acute lower respiratory infections in Cambodia.

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    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Arnott A,Vong S,Rith S,Naughtin M,Ly S,Guillard B,Deubel V,Buchy P

    更新日期:2013-03-01 00:00:00

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    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Učakar V,Sočan M,Trilar KP

    更新日期:2013-11-01 00:00:00

  • Safety and immunogenicity of Sinovac's prototype pandemic influenza H5N1 vaccines: a review on clinical trials.

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    pub_type: 杂志文章,随机对照试验,评审


    authors: Qiu YZ,Yin WD

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  • Evidence of reassortment of pandemic H1N1 influenza virus in swine in Argentina: are we facing the expansion of potential epicenters of influenza emergence?

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    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Pereda A,Rimondi A,Cappuccio J,Sanguinetti R,Angel M,Ye J,Sutton T,Dibárbora M,Olivera V,Craig MI,Quiroga M,Machuca M,Ferrero A,Perfumo C,Perez DR

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    authors: Belshe RB,Toback SL,Yi T,Ambrose CS

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    pub_type: 杂志文章


    authors: Bulimo WD,Garner JL,Schnabel DC,Bedno SA,Njenga MK,Ochieng WO,Amukoye E,Magana JM,Simwa JM,Ofula VO,Lifumo SM,Wangui J,Breiman RF,Martin SK

    更新日期:2008-05-01 00:00:00

  • Influenza vaccination coverage and effectiveness in young children in Thailand, 2011-2013.

    abstract:BACKGROUND:Since 2009, Thailand has recommended influenza vaccine for children aged 6 months through 2 years, but no estimates of influenza vaccine coverage or effectiveness are available for this target group. METHODS:During August 2011-May 2013, high-risk and healthy children aged ≤36 months were enrolled in a 2-yea...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Kittikraisak W,Suntarattiwong P,Levy J,Fernandez S,Dawood FS,Olsen SJ,Chotpitayasunondh T

    更新日期:2015-03-01 00:00:00

  • Mortality burden from seasonal influenza and 2009 H1N1 pandemic influenza in Beijing, China, 2007-2013.

    abstract:BACKGROUND:Data about influenza mortality burden in northern China are limited. This study estimated mortality burden in Beijing associated with seasonal influenza from 2007 to 2013 and the 2009 H1N1 pandemic. METHODS:We estimated influenza-associated excess mortality by fitting a negative binomial model using weekly ...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Wu S,Wei Z,Greene CM,Yang P,Su J,Song Y,Iuliano AD,Wang Q

    更新日期:2018-01-01 00:00:00

  • Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl.

    abstract::The ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimental...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Lebarbenchon C,Pantin-Jackwood M,Kistler WM,Luttrell MP,Spackman E,Stallknecht DE,Brown JD

    更新日期:2013-11-01 00:00:00

  • Assessment of the benefits of seasonal influenza vaccination: Elements of a framework to interpret estimates of vaccine effectiveness and support robust decision-making and communication.

    abstract::Systematic reviews and meta-analyses confirm that influenza vaccination reduces the risk of influenza illness by between about 40% and 60% in seasons when circulating influenza stains are well matched to vaccine strains. Influenza vaccine effectiveness (IVE) estimates, however, are often discordant and a source of con...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章,评审


    authors: Hollingsworth R,El Guerche-Séblain C,Tsai T,Vasiliev Y,Lee S,Bright H,Barbosa P

    更新日期:2021-01-01 00:00:00

  • Long-term vaccine-induced heterologous protection against H5N1 influenza viruses in the ferret model.

    abstract:BACKGROUND:Highly pathogenic H5N1 influenza viruses reemerged in humans in 2003 and have caused fatal human infections in Asia and Africa as well as ongoing outbreaks in poultry. These viruses have evolved substantially and are now so antigenically varied that a single vaccine antigen may not protect against all circul...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Ducatez MF,Webb A,Crumpton JC,Webby RJ

    更新日期:2013-07-01 00:00:00

  • Health-seeking behaviors of patients with acute respiratory infections during the outbreak of novel coronavirus disease 2019 in Wuhan, China.

    abstract::We conducted two surveys to evaluate the health-seeking behaviors of individuals with acute respiratory infections (ARI) during the COVID-19 outbreak in Wuhan, China. Among 351 participants reporting ARI (10.3%, 351/3,411), 36.5% sought medical assistance. Children were more likely to seek medical assistance than othe...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Yang J,Gong H,Chen X,Chen Z,Deng X,Qian M,Hou Z,Ajelli M,Viboud C,Yu H

    更新日期:2020-09-10 00:00:00

  • Influenza symptoms and their impact on elderly adults: randomised trial of AS03-adjuvanted or non-adjuvanted inactivated trivalent seasonal influenza vaccines.

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    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章,随机对照试验


    authors: van Essen GA,Beran J,Devaster JM,Durand C,Duval X,Esen M,Falsey AR,Feldman G,Gervais P,Innis BL,Kovac M,Launay O,Leroux-Roels G,McElhaney JE,McNeil S,Oujaa M,Richardus JH,Ruiz-Palacios G,Osborne RH,Oostvogels L

    更新日期:2014-07-01 00:00:00

  • Estimating the secondary attack rate and serial interval of influenza-like illnesses using social media.

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    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Yom-Tov E,Johansson-Cox I,Lampos V,Hayward AC

    更新日期:2015-07-01 00:00:00

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    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Bearden A,Friedrich TC,Goldberg TL,Byrne B,Spiegel C,Schult P,Safdar N

    更新日期:2012-09-01 00:00:00

  • Review on the impact of pregnancy and obesity on influenza virus infection.

    abstract::A myriad of risk factors have been linked to an increase in the severity of the pandemic H1N1 2009 influenza A virus [A(H1N1)pdm09] including pregnancy and obesity where death rates can be elevated as compared to the general population. The goal of this review is to provide an overview of the influence of pregnancy an...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章,评审


    authors: Karlsson EA,Marcelin G,Webby RJ,Schultz-Cherry S

    更新日期:2012-11-01 00:00:00

  • Matrix-M adjuvanted virosomal H5N1 vaccine confers protection against lethal viral challenge in a murine model.

    abstract:BACKGROUND:A candidate pandemic influenza H5N1 vaccine should provide rapid and long-lasting immunity against antigenically drifted viruses. As H5N1 viruses are poorly immunogenic, this may require a combination of immune potentiating strategies. An attractive approach is combining the intrinsic immunogenicity of viros...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Pedersen G,Major D,Roseby S,Wood J,Madhun AS,Cox RJ

    更新日期:2011-11-01 00:00:00

  • Frequency and patterns of contact with domestic poultry and potential risk of H5N1 transmission to humans living in rural Cambodia.

    abstract:BACKGROUND:Since 2004, H5N1 outbreaks have been recurrent in domestic poultry and humans in Cambodia. To date, seven human cases (100% CFR) and 22 outbreaks in poultry have been confirmed. Household ownership of backyard poultry (FAO Sector 4 poultry production) in rural Cambodia is high. An understanding of the extent...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章


    authors: Van Kerkhove MD,Ly S,Holl D,Guitian J,Mangtani P,Ghani AC,Vong S

    更新日期:2008-09-01 00:00:00

  • Influenza antivirals currently in late-phase clinical trial.

    abstract::Influenza antiviral drugs are important for the control of influenza, most specifically for the treatment of influenza patients with severe disease following infection with a seasonal influenza virus, a newly emerging influenza strain, or in the event of a pandemic. Many influenza antivirals that are currently under i...

    journal_title:Influenza and other respiratory viruses

    pub_type: 杂志文章,评审


    authors: Koszalka P,Tilmanis D,Hurt AC

    更新日期:2017-05-01 00:00:00