Abstract：
:Some species of clover are reported to have beneficial effects in human diseases. However, little is known about the activity of the forage plant Trifolium repens, or white clover, which has been recently found to exert a hepatoprotective action. Scientific interest is increasingly focused on identifying new drugs, especially natural products and their derivatives, to treat human diseases including cancer. We analyzed the anticancer effects of T. repens in several cancer cell lines. The phytochemical components of T. repens were first extracted in a methanol solution and then separated into four fractions by ultra-high-performance liquid chromatography. The effects of the total extract and each fraction on cancer cell proliferation were analyzed by MTT assay and Western blotting. T. repens and, more robustly, its isoflavonoid-rich fraction showed high cytotoxic effects in chronic myelogenous leukemia (CML) K562 cells, with IC50 values of 1.67 and 0.092 mg/mL, respectively. The block of cell growth was associated with a total inhibition of BCR-ABL/STAT5 and activation of the p38 signaling pathways. In contrast, these strongly cytotoxic effects did not occur in normal cells. Our findings suggest that the development of novel compounds derived from phytochemical molecules contained in Trifolium might lead to the identification of new therapeutic agents active against CML.
journal_name
Cellsjournal_title
Cellsauthors
Sarno F,Pepe G,Termolino P,Carafa V,Massaro C,Merciai F,Campiglia P,Nebbioso A,Altucci Ldoi
10.3390/cells9020379subject
Has Abstractpub_date
2020-02-06 00:00:00issue
2issn
2073-4409pii
cells9020379journal_volume
9pub_type
杂志文章相关文献
Cells文献大全abstract：:Chronic myeloid leukemia (CML) has been a "model disease" with a long history. Beginning with the first discovery of leukemia and the description of the Philadelphia Chromosome and ending with the current goal of achieving treatment-free remission after targeted therapies, we describe here the journey of CML, focusing...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells10010117
更新日期：2021-01-10 00:00:00
abstract：:Circulating tumor cells (CTCs) as real-time liquid biopsy [...]. ...
journal_title：Cells
pub_type： 社论
doi：10.3390/cells9030661
更新日期：2020-03-09 00:00:00
abstract：:One of the crucial challenges in the clinical management of cancer is resistance to chemotherapeutics. Multidrug resistance (MDR) has been intensively studied, and one of the most prominent mechanisms underlying MDR is overexpression of adenosine triphosphate (ATP)-binding cassette (ABC) transporters. Despite research...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells7080107
更新日期：2018-08-14 00:00:00
abstract：:Gene editing permits changing specific DNA sequences within the vast genomes of human cells. Stem cells are particularly attractive targets for gene editing interventions as their self-renewal and differentiation capabilities consent studying cellular differentiation processes, screening small-molecule drugs, modeling...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9040953
更新日期：2020-04-13 00:00:00
abstract：:Xanthohumol (XN), a prenylated chalcone from hops, has been reported to exhibit a variety of health-beneficial effects. However, poor bioavailability may limit its application in the prevention and therapy of diseases. The objective of this study was to determine whether a micellar solubilization of xanthohumol could ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8040359
更新日期：2019-04-17 00:00:00
abstract：:Calcific aortic valve stenosis (CAVS) is a common age-related disease characterized by active calcification of the leaflets of the aortic valve. How innate immune cells are involved in disease pathogenesis is not clear. In this study we investigate the role of the pattern recognition receptor Toll-like receptor 7 (TLR...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071710
更新日期：2020-07-16 00:00:00
abstract：:The experimental determination of the relative biological effectiveness of thermal neutron factors is fundamental in Boron Neutron Capture Therapy. The present values have been obtained while using mixed beams that consist of both neutrons and photons of various energies. A common weighting factor has been used for bo...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9102144
更新日期：2020-09-23 00:00:00
abstract：:Telomere dysfunction has been strongly implicated in the initiation of genomic instability and is suspected to be an early event in the carcinogenesis of human solid tumors. Recent findings have established the presence of telomere fusions in human breast and prostate malignancies; however, the onset of this genomic i...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8050414
更新日期：2019-05-04 00:00:00
abstract：:Transient receptor potential classical or canonical 4 (TRPC4) and TRPC5 channels are members of the classical or canonical transient receptor potential (TRPC) channel family of non-selective cation channels. TRPC4 and TRPC5 channels are widely accepted as receptor-operated cation channels that are activated in a phosp...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells7110218
更新日期：2018-11-20 00:00:00
abstract：:Embryoid bodies (EBs) resemble self-organizing aggregates of pluripotent stem cells that recapitulate some aspects of early embryogenesis. Within few days, the cells undergo a transition from rather homogeneous epithelial-like pluripotent stem cell colonies into a three-dimensional organization of various cell types w...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9102270
更新日期：2020-10-11 00:00:00
abstract：:Epidermal growth factor receptor (EGFR) is the first discovered member of the receptor tyrosine kinase superfamily and plays a fundamental role during embryogenesis and in adult tissues, being involved in growth, differentiation, maintenance and repair of various tissues and organs. The role of EGFR in the regulation ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9081887
更新日期：2020-08-12 00:00:00
abstract：:Siglecs (Sialic acid-binding immunoglobulin-type lectins) are a I-type lectin that typically binds sialic acid. Siglecs are predominantly expressed in immune cells and generate activating or inhibitory signals. They are also shown to be expressed on the surface of cells in the nervous system and have been shown to pla...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8101125
更新日期：2019-09-22 00:00:00
abstract：:Over a decade ago, the formation of neutrophil extracellular traps (NETs) was described as a novel mechanism employed by neutrophils to tackle infections. Currently applied methods for NETs release quantification are often limited by the use of unspecific dyes and technical difficulties. Therefore, we aimed to develop...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9020508
更新日期：2020-02-24 00:00:00
abstract：:Background: Mucopolysaccharidosis type I-Hurler (MPS1-H) is a severe genetic lysosomal storage disorder due to loss-of-function mutations in the IDUA gene. The subsequent complete deficiency of alpha l-iduronidase enzyme is directly responsible of a progressive accumulation of glycosaminoglycans (GAG) in lysosomes whi...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9122593
更新日期：2020-12-03 00:00:00
abstract：:The replication independent (RI) histone H2A.Z is one of the more extensively studied variant members of the core histone H2A family, which consists of many replication dependent (RD) members. The protein has been shown to be indispensable for survival, and involved in multiple roles from DNA damage to chromosome segr...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9051167
更新日期：2020-05-08 00:00:00
abstract：:Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by memory loss and multiple cognitive impairments. Several decades of intense research have revealed that multiple cellular changes are implicated in the development and progression of AD, including mitochondrial damage, synaptic dysfunc...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8050488
更新日期：2019-05-22 00:00:00
abstract：:The NF-κB transcription factor pathway is a crucial regulator of inflammation and immune responses. Additionally, aberrant NF-κB signaling has been identified in many types of cancer. Downstream of key oncogenic pathways, such as RAS, BCR-ABL, and Her2, NF-κB regulates transcription of target genes that promote cell s...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells5020016
更新日期：2016-04-06 00:00:00
abstract：:The turnip Brassica rapa has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are targets of CULLIN3-bas...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8040336
更新日期：2019-04-10 00:00:00
abstract：:Type 1 diabetes (T1D) is an autoimmune condition where the body's immune cells destroy their insulin-producing pancreatic beta cells leading to dysregulated glycaemia. Individuals with T1D control their blood glucose through exogenous insulin replacement therapy, often using multiple daily injections or pumps. However...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9112403
更新日期：2020-11-03 00:00:00
abstract：:Dendritic cells and macrophages are common components of the tumour immune microenvironment and can contribute to immune suppression in both solid and haematological cancers. The Bone Morphogenetic Protein (BMP) pathway has been reported to be involved in cancer, and more recently in leukaemia development and progress...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8070722
更新日期：2019-07-14 00:00:00
abstract：:Nuclear lamins (NLs) are essential components of the animal cell nucleus involved in the regulation of a plethora of molecular and cellular processes. These include the nuclear envelope assembly and stability, mechanotransduction and chromatin organization, transcription, DNA replication, damage repair, and genomic in...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9061340
更新日期：2020-05-27 00:00:00
abstract：:Monoglyceride lipase (MGLL) regulates metabolism by catabolizing monoacylglycerols (MAGs), including the endocannabinoid 2-arachidonoyl glycerol (2-AG) and some of its bioactive congeners, to the corresponding free fatty acids. Mgll knockout mice (Mgll-/-) exhibit elevated tissue levels of MAGs in association with res...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9122705
更新日期：2020-12-17 00:00:00
abstract：:Anchoring of heterochromatin to the nuclear envelope appears to be an important process ensuring the spatial organization of the chromatin structure and genome function in eukaryotic nuclei. Proteins of the inner nuclear membrane (INM) mediating these interactions are able to recognize lamina-associated heterochromati...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells7020011
更新日期：2018-02-06 00:00:00
abstract：:The failure of drug efficacy in clinical trials remains a big issue in cancer research. This is largely due to the limitations of two-dimensional (2D) cell cultures, the most used tool in drug screening. Nowadays, three-dimensional (3D) cultures, including spheroids, are acknowledged to be a better model of the in viv...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9030703
更新日期：2020-03-13 00:00:00
abstract：:Mycobacterium bovis (M. bovis) is a member of the Mycobacterium tuberculosis (Mtb) complex causing bovine tuberculosis (TB) and imposing a high zoonotic threat to human health. Kallikreins (KLKs) belong to a subgroup of secreted serine proteases. As their role is established in various physiological and pathological p...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8050415
更新日期：2019-05-05 00:00:00
abstract：:More than 50 years after the first description of Bronchopulmonary dysplasia (BPD) by Northway, this chronic lung disease affecting many preterm infants is still poorly understood. Additonally, approximately 40% of preterm infants suffering from severe BPD also suffer from Bronchopulmonary dysplasia-associated pulmona...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9081875
更新日期：2020-08-11 00:00:00
abstract：:(1) Aim: In the present paper we analyzed the transcriptome of CSCs (Cancer Stem Cells), in order to find defining molecular processes of breast cancer. (2) Methods: We performed RNA-Seq from CSCs isolated from the basal cell line MDA-MB-468. Enriched processes and networks were studied using the IPA (Ingenuity Pathwa...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9030709
更新日期：2020-03-13 00:00:00
abstract：:The bioactive lipid sphingosine-1-phosphate (S1P), along with its receptors, modulates lymphocyte trafficking and immune responses to regulate skin inflammation. Macrophages are important in the pathogenesis of psoriasiform skin inflammation and express various S1P receptors. How they respond to S1P in skin inflammati...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8080785
更新日期：2019-07-28 00:00:00
abstract：:Podoplanin and CD44 are transmembrane glycoproteins involved in inflammation and cancer. In this paper, we report that podoplanin is coordinately expressed with the CD44 standard (CD44s) and variant (CD44v) isoforms in vivo-in hyperplastic skin after a pro-inflammatory stimulus with 12-O-tetradecanoylphorbol-13-acetat...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9102200
更新日期：2020-09-29 00:00:00
abstract：:More than 50% of human cancers harbor TP53 mutations and increased expression of Mouse double minute 2 homolog(MDM2), which contribute to cancer progression and drug resistance. Renal cell carcinoma (RCC) has an unusually high incidence of wild-type p53, with a mutation rate of less than 4%. MDM2 is master regulator o...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9061475
更新日期：2020-06-16 00:00:00
