Abstract：
:Some species of clover are reported to have beneficial effects in human diseases. However, little is known about the activity of the forage plant Trifolium repens, or white clover, which has been recently found to exert a hepatoprotective action. Scientific interest is increasingly focused on identifying new drugs, especially natural products and their derivatives, to treat human diseases including cancer. We analyzed the anticancer effects of T. repens in several cancer cell lines. The phytochemical components of T. repens were first extracted in a methanol solution and then separated into four fractions by ultra-high-performance liquid chromatography. The effects of the total extract and each fraction on cancer cell proliferation were analyzed by MTT assay and Western blotting. T. repens and, more robustly, its isoflavonoid-rich fraction showed high cytotoxic effects in chronic myelogenous leukemia (CML) K562 cells, with IC50 values of 1.67 and 0.092 mg/mL, respectively. The block of cell growth was associated with a total inhibition of BCR-ABL/STAT5 and activation of the p38 signaling pathways. In contrast, these strongly cytotoxic effects did not occur in normal cells. Our findings suggest that the development of novel compounds derived from phytochemical molecules contained in Trifolium might lead to the identification of new therapeutic agents active against CML.
journal_name
Cellsjournal_title
Cellsauthors
Sarno F,Pepe G,Termolino P,Carafa V,Massaro C,Merciai F,Campiglia P,Nebbioso A,Altucci Ldoi
10.3390/cells9020379subject
Has Abstractpub_date
2020-02-06 00:00:00issue
2issn
2073-4409pii
cells9020379journal_volume
9pub_type
杂志文章相关文献
Cells文献大全abstract：:The γ-aminobutyric acid type A receptor-associated protein (GABARAP) and its close paralogs GABARAPL1 and GABARAPL2 constitute a subfamily of the autophagy-related 8 (Atg8) protein family. Being associated with a variety of dynamic membranous structures of autophagic and non-autophagic origin, Atg8 proteins functional...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9051296
更新日期：2020-05-22 00:00:00
abstract：:The disease course of myelodysplastic syndromes (MDS) features chromosome instability and clonal evolution, leading to the sequential acquisition of novel cytogenetic aberrations and the accumulation of these abnormalities in the bone marrow. Although clonal cytogenetic abnormalities can be detected by conventional cy...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8040304
更新日期：2019-04-02 00:00:00
abstract：:For decades, cardiovascular disease (CVD) has been the leading cause of death throughout most developed countries. Several studies relate RNA splicing, and more recently also circular RNAs (circRNAs), to CVD. CircRNAs originate from linear transcripts and have been shown to exhibit tissue-specific expression profiles....
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071616
更新日期：2020-07-04 00:00:00
abstract：:Cancer is a complex expression of an altered state of cellular differentiation associated with severe clinical repercussions. The effort to characterize this pathological entity to understand its underlying mechanisms and visualize potential therapeutic strategies has been constant. In this context, some cellular (enh...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9071612
更新日期：2020-07-03 00:00:00
abstract：:Induced pluripotent stem cells (iPSCs) have revolutionized the study of human diseases as they can renew indefinitely, undergo multi-lineage differentiation, and generate disease-specific models. However, the difficulty of working with iPSCs is that they are prone to genetic instability. Furthermore, genetically unsta...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8091068
更新日期：2019-09-11 00:00:00
abstract：:The most common cystic fibrosis-causing mutation (F508del, present in ~85% of CF patients) leads to CFTR misfolding, which is recognized by the endoplasmic reticulum (ER) quality control (ERQC), resulting in ER retention and early degradation. It is known that CFTR exit from the ER is mediated by specific retention/so...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8040353
更新日期：2019-04-14 00:00:00
abstract：:Substantial variations in differentiation properties have been reported among human pluripotent cell lines (hPSC), which could affect their utility and clinical safety. We characterized the variable osteogenic capacity observed between different human pluripotent stem cell lines. By focusing on the miRNA expression pr...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8121523
更新日期：2019-11-26 00:00:00
abstract：:In this study we report the functional comparison of T cell receptor (TCR)-engineered major histocompatibility complex (MHC) class I-restricted CD4+ versus CD8+ T cells targeting a peptide from six transmembrane epithelial antigen of the prostate 1 (STEAP1) in the context of HLA-A*02:01. STEAP1 is a tumor-associated a...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071581
更新日期：2020-06-29 00:00:00
abstract：:In the neonate forebrain, network formation is driven by the spontaneous synchronized activity of pyramidal cells and interneurons, consisting of bursts of electrical activity and intracellular Ca2+ oscillations. By employing ratiometric Na+ imaging in tissue slices obtained from animals at postnatal day 2-4 (P2-4), w...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9010102
更新日期：2019-12-31 00:00:00
abstract：:This review briefly summarizes the single cell application of classical chemical dyes used to visualize cardiomyocyte physiology and their undesirable toxicities which have the potential to confound experimental observations. We will discuss, in detail, the more recent iterative development of fluorescent and biolumin...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells7060051
更新日期：2018-05-31 00:00:00
abstract：:Nonalcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver diseases with an increasing prevalence due to rising rates of obesity, metabolic syndrome and type II diabetes. Untreated NAFLD may progress to steatohepatitis (NASH) and ultimately liver cirrhosis. NAFLD is characterized by lipid accumulatio...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9061346
更新日期：2020-05-28 00:00:00
abstract：:The transcription factor Snail triggers epithelial-to-mesenchymal transition (EMT), endowing cancer cells with invasive properties during tumor progression. Extracellular vesicles (EVs) released from cancer cells at various stages of cancer progression are known to influence the tumor pre-metastatic niche and metastat...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071585
更新日期：2020-06-30 00:00:00
abstract：:ELISpot is one of the most commonly used immune monitoring assays, which allows the functional assessment of the immune system at the single cell level. With its outstanding sensitivity and ease of performance, the assay has recently advanced from the mere single function cell analysis to multifunctional analysis by i...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells3041102
更新日期：2014-11-27 00:00:00
abstract：OBJECTIVES:Synovial fluids of rheumatoid arthritis (RA) patients commonly contain high concentrations of soluble CD14 (sCD14). To investigate its potential role in RA pathogenesis, we tested whether sCD14 binding transmits a signal to fibroblast-like synoviocytes from RA patients (RA-FLS). METHODS:The induction of pro...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071689
更新日期：2020-07-14 00:00:00
abstract：:Accumulating studies report that microRNAs (miRNAs) are actively involved in skeletal myogenesis. Previously, our study revealed that miR-146b-3p was related to the growth of skeletal muscle. Here, we further report that miR-146b-3p is essential for the proliferation, differentiation, and apoptosis of chicken myoblast...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8070656
更新日期：2019-06-29 00:00:00
abstract：:Sarcopenia, a syndrome commonly seen in elderly populations, is often characterized by a gradual loss of skeletal muscle, leading to the decline of muscle strength and physical performance. Growing evidence suggests that the prevalence of sarcopenia increases in patients with heart failure (HF), which is a dominant pa...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9102284
更新日期：2020-10-13 00:00:00
abstract：:Lysyl oxidase (LOX) proteins comprise a family of five copper-dependent enzymes (LOX and four LOX-like isoenzymes (LOXL1-4)) critical for extracellular matrix (ECM) homeostasis and remodeling. The primary role of LOX enzymes is to oxidize lysyl and hydroxylysyl residues from collagen and elastin chains into highly rea...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8121483
更新日期：2019-11-21 00:00:00
abstract：:Glioblastomas are the most frequent and aggressive form of primary brain tumors with no efficient cure. However, they often exhibit specific metabolic shifts that include deficiency in the biosynthesis of and dependence on certain exogenous amino acids. Here, we evaluated, in vitro, a novel combinatory antiglioblastom...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9102217
更新日期：2020-09-30 00:00:00
abstract：:Breast cancer-related mortality remains high worldwide, despite tremendous advances in diagnostics and therapeutics; hence, the quest for better strategies for disease management, as well as the identification of modifiable risk factors, continues. With recent leaps in genomic technologies, microbiota have emerged as ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9051091
更新日期：2020-04-28 00:00:00
abstract：:Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the diffe...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells6030018
更新日期：2017-07-03 00:00:00
abstract：:Transcriptional coactivator with a PDZ-binding motif (TAZ) is one of the mammalian orthologs of Drosophila Yorkie, a transcriptional coactivator of the Hippo pathway. TAZ has been suggested to function as a regulator that modulates the expression of cell proliferation and anti-apoptotic genes in order to stimulate cel...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9010171
更新日期：2020-01-09 00:00:00
abstract：:We have previously discovered that HDAC6 regulates the DNA damage response (DDR) via modulating the homeostasis of a DNA mismatch repair protein, MSH2, through HDAC6's ubiquitin E3 ligase activity. Here, we have reported HDAC6's second potential E3 ligase substrate, a critical cell cycle checkpoint protein, Chk1. We h...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9102237
更新日期：2020-10-04 00:00:00
abstract：:Tissue-resident macrophages play critical roles in controlling homeostasis, tissue repair, and immunity. Inflammatory macrophages can sustain tissue damage and promote the development of fibrosis during infections and sterile tissue injury. The NLRP3 inflammasome and its effector cytokine IL-1β have been identified as...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9010079
更新日期：2019-12-28 00:00:00
abstract：:It is well known that fibroblast growth factor receptor 2 (FGFR2) interacts with its ligand of fibroblast growth factor (FGF) therefore exerting biological functions on cell proliferation and differentiation. In this study, we first reported that the FGFR2 gene could generate a circular RNA of circFGFR2, which regulat...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells7110199
更新日期：2018-11-06 00:00:00
abstract：:The transport of host proteins into and out of the nucleus is key to host function. However, nuclear transport is restricted by nuclear pores that perforate the nuclear envelope. Protein intrinsic disorder is an inherent feature of this selective transport barrier and is also a feature of the nuclear transport recepto...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9122654
更新日期：2020-12-10 00:00:00
abstract：:Cancer frequency and prevalence have been increasing in the past decades, with devastating impacts on patients and their families. Despite the great advances in targeted approaches, there is still a lack of methods to predict individual patient responses, and therefore treatments are tailored according to average resp...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9020293
更新日期：2020-01-25 00:00:00
abstract：:EGFR and some of the cognate ligands extensively traffic in extracellular vesicles (EVs) from different biogenesis pathways. EGFR belongs to a family of four homologous tyrosine kinase receptors (TKRs). This family are one of the major drivers of cancer and is involved in several of the most frequent malignancies such...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9112505
更新日期：2020-11-19 00:00:00
abstract：:The endoplasmic reticulum (ER) stress response and autophagy are important cellular responses that determine cell fate and whose dysregulation is implicated in the perturbation of homeostasis and diseases. Tonicity-responsive enhancer-binding protein (TonEBP, also called NFAT5) is a pleiotropic stress protein that med...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9091928
更新日期：2020-08-20 00:00:00
abstract：BACKGROUND:Regenerative therapies based on autologous mesenchymal stem cells (MSC) as well as stem cells in general are still facing an unmet need for non-invasive sampling, availability, and scalability. The only known adult source of autologous MSCs permanently available with no pain, discomfort, or infection risk is...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9092069
更新日期：2020-09-10 00:00:00
abstract：:Peripheral nerve injury (PNI) leads to the loss of motor, sensory, and autonomic functions, and often triggers neuropathic pain. During the last years, many efforts have focused on finding new therapies to increase axonal regeneration or to alleviate painful conditions. Still only a few of them have targeted both phen...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9040808
更新日期：2020-03-27 00:00:00
