Abstract：
:(1) Aim: In the present paper we analyzed the transcriptome of CSCs (Cancer Stem Cells), in order to find defining molecular processes of breast cancer. (2) Methods: We performed RNA-Seq from CSCs isolated from the basal cell line MDA-MB-468. Enriched processes and networks were studied using the IPA (Ingenuity Pathway Analysis) tool. Validation was performed with qRT-PCR and the analysis of relevant genes was evaluated by overexpression, flow cytometry and in vivo zebrafish studies. Finally, the clinical relevance of these results was assessed using reported cohorts. (3) Results: We found that CSCs presented marked differences from the non-CSCs, including enrichment in transduction cascades related to stemness, cellular growth, proliferation and apoptosis. Interestingly, CSCs overexpressed a module of co-regulated Chromosomal Passenger Proteins including BIRC5 (survivin), INCENP and AURKB. Overexpression of BIRC5 increased the number of CSCs, as assessed by in vitro and in vivo zebrafish xenotransplant analyses. Analysis of previously published cohorts showed that this co-regulated module was not only overexpressed in basal breast tumors but also associated with relapse-free and overall survival in these patients. (4) Conclusions: These results underline the importance of Cancer Stem Cells in breast cancer progression and point toward the possible use of chromosomal passenger proteins as prognostic factors.
journal_name
Cellsjournal_title
Cellsauthors
Schwarz-Cruz Y Celis A,Ceballos-Cancino G,Vazquez-Santillan K,Espinosa M,Zampedri C,Bahena I,Ruiz V,Maldonado V,Melendez-Zajgla Jdoi
10.3390/cells9030709subject
Has Abstractpub_date
2020-03-13 00:00:00issue
3issn
2073-4409pii
cells9030709journal_volume
9pub_type
杂志文章相关文献
Cells文献大全abstract：:Aquaporins (AQPs) facilitate transmembrane water and solute transport, and in addition to contributing to transepithelial water transport, they safeguard cell volume homeostasis. This study examined the expression and localization of AQP1 and AQP3 in the gills of Japanese medaka (Oryzias latipes) in response to osmoti...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8050422
更新日期：2019-05-08 00:00:00
abstract：:We present a hiPSC-based 3D in vitro system suitable to test neurotoxicity (NT). Human iPSCs-derived 3D neurospheres grown in 96-well plate format were characterized timewise for 6-weeks. Changes in complexity and homogeneity were followed by immunocytochemistry and transmission electron microscopy. Transcriptional ac...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9051122
更新日期：2020-05-01 00:00:00
abstract：:White adipose tissue (WAT) lipolysis contributes to energy balance during fasting. Lipolysis can proceed by the sequential hydrolysis of triglycerides (TGs) by adipose triglyceride lipase (ATGL), then of diacylglycerols (DGs) by hormone-sensitive lipase (HSL). We showed that the combined genetic deficiency of ATGL and...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8050395
更新日期：2019-04-29 00:00:00
abstract：:For years, moderate hypothermia (32 °C) has been proposed as an unorthodox therapy for liver injuries, with proven hepatoprotective potential. Yet, limited mechanistic understanding has largely denied its acceptance over conventional pharmaceuticals for hepatoprotection. Today, facing a high prevalence of acetaminophe...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9112354
更新日期：2020-10-26 00:00:00
abstract：:Sarcopenia, a syndrome commonly seen in elderly populations, is often characterized by a gradual loss of skeletal muscle, leading to the decline of muscle strength and physical performance. Growing evidence suggests that the prevalence of sarcopenia increases in patients with heart failure (HF), which is a dominant pa...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9102284
更新日期：2020-10-13 00:00:00
abstract：:Non-human primates (NHP) are important surrogate models for late preclinical development of advanced therapy medicinal products (ATMPs), including induced pluripotent stem cell (iPSC)-based therapies, which are also under development for heart failure repair. For effective heart repair by remuscularization, large numb...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9061349
更新日期：2020-05-29 00:00:00
abstract：:The co-expression of androgen (AR) and estrogen (ER) receptors, in terms of higher AR/ER ratio, has been recently associated with poor outcome in ER-positive (ER+) breast cancer (BC) patients. The aim of this study was to analyze if the biological aggressiveness, underlined in ER+ BC tumors with higher AR/ER ratio, co...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9041064
更新日期：2020-04-24 00:00:00
abstract：:The aggregation of proteins compromises cell fitness, either because it titrates functional proteins into non-productive inclusions or because it results in the formation of toxic assemblies. Accordingly, computational proteome-wide analyses suggest that prevention of aggregation upon misfolding plays a key role in se...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8080856
更新日期：2019-08-08 00:00:00
abstract：:Autophagy is a ubiquitous eukaryotic process that also occurs in trypanosomatid parasites, protist organisms belonging to the supergroup Excavata, distinct from the supergroup Opistokontha that includes mammals and fungi. Half of the known yeast and mammalian AuTophaGy (ATG) proteins were detected in trypanosomatids, ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells1030346
更新日期：2012-07-27 00:00:00
abstract：:Rab proteins compose the largest family of small GTPases and control the different steps of intracellular membrane traffic. More recently, they have been shown to also regulate cell signaling, division, survival, and migration. The regulation of these processes generally occurs through recruitment of effectors and reg...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8080909
更新日期：2019-08-16 00:00:00
abstract：:T cells play an essential role in the pathogenesis of both human rheumatoid arthritis (RA) and its murine models. A key molecule in T cell activation is ZAP-70, therefore we aimed to investigate the effects of partial ZAP-70 deficiency on the pathogenesis of recombinant human G1(rhG1)-induced arthritis (GIA), a well-e...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8050504
更新日期：2019-05-24 00:00:00
abstract：:The generation of new ribosomes is a coordinated process essential to sustain cell growth. As such, it is tightly regulated according to cell needs. As cancer cells require intense protein translation to ensure their enhanced growth rate, they exploit various mechanisms to boost ribosome biogenesis. In this review, we...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8091098
更新日期：2019-09-17 00:00:00
abstract：:Sepsis is a leading cause of death in intensive care units, and cardiac dysfunction is an identified serious component of the multi-organ failure associated with this critical condition. This review summarized the current discoveries and hypothesizes of how autophagy changes in the heart during sepsis and the underlyi...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8020141
更新日期：2019-02-10 00:00:00
abstract：:Dendritic cells and macrophages are common components of the tumour immune microenvironment and can contribute to immune suppression in both solid and haematological cancers. The Bone Morphogenetic Protein (BMP) pathway has been reported to be involved in cancer, and more recently in leukaemia development and progress...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8070722
更新日期：2019-07-14 00:00:00
abstract：:The disease course of myelodysplastic syndromes (MDS) features chromosome instability and clonal evolution, leading to the sequential acquisition of novel cytogenetic aberrations and the accumulation of these abnormalities in the bone marrow. Although clonal cytogenetic abnormalities can be detected by conventional cy...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8040304
更新日期：2019-04-02 00:00:00
abstract：:In response to inflammatory stimuli, immune cells reconfigure their metabolism and bioenergetics to generate energy and substrates for cell survival and to launch immune effector functions. As a critical component of the innate immune system, the nucleotide-binding and oligomerization domain, leucine-rich repeat, and ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9081808
更新日期：2020-07-30 00:00:00
abstract：:We have previously shown that the combination of radiotherapy with human umbilical-cord-derived mesenchymal stromal/stem cells (MSCs) cell therapy significantly reduces the size of the xenotumors in mice, both in the directly irradiated tumor and in the distant nonirradiated tumor or its metastasis. We have also shown...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9092015
更新日期：2020-09-02 00:00:00
abstract：:Langerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studies of BG have histor...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9040974
更新日期：2020-04-15 00:00:00
abstract：:The PIKfyve inhibitor apilimod is currently undergoing clinical trials for treatment of COVID-19. However, although apilimod might prevent viral invasion by inhibiting host cell proteases, the same proteases are critical for antigen presentation leading to T cell activation and there is good evidence from both in vitr...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells10010030
更新日期：2020-12-27 00:00:00
abstract：:MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are responsible for RNA silencing and post-transcriptional regulation of gene expression. They can mediate a fine-tuned crosstalk among coding and non-coding RNA molecules sharing miRNA response elements (MREs). In a suitable environment, both coding and n...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9071574
更新日期：2020-06-28 00:00:00
abstract：:Metabolic remodelling of the tumour microenvironment is a major mechanism by which cancer cells survive and resist treatment. The pro-oncogenic inflammatory cascade released by adipose tissue promotes oncogenic transformation, proliferation, angiogenesis, metastasis and evasion of apoptosis. STAT3 has emerged as an im...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9041043
更新日期：2020-04-22 00:00:00
abstract：:Angiopoietin-1 (Ang-1) is a ligand of Tie-2 receptors that promotes angiogenesis. It has been established that regulatory loops exist between angiogenic growth factors and distinct pro or anti-angiogenic miRNAs, but the nature and the roles of Ang-1-regulated miRNAs remain unclear. In this study, we assessed the role ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071602
更新日期：2020-07-02 00:00:00
abstract：:While high levels of saturated fatty acids are associated with impairment of cardiovascular functions, n-3 polyunsaturated fatty acids (PUFAs) have been shown to exert protective effects. However the molecular mechanisms underlying this evidence are not completely understood. In the present study we have used rat H9c2...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9030537
更新日期：2020-02-26 00:00:00
abstract：:Pharmaco-therapeutic strategies of atrial fibrillation (AF) are moderately effective and do not prevent AF onset and progression. Therefore, there is an urgent need to develop novel therapies. Previous studies revealed heat shock protein (HSP)-inducing compounds to mitigate AF onset and progression. Such an HSP induci...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071729
更新日期：2020-07-20 00:00:00
abstract：:Circular RNAs (circRNAs) belong to the ever-growing class of naturally occurring noncoding RNAs (ncRNAs) molecules. Unlike linear RNA, circRNAs are covalently closed transcripts mostly generated from precursor-mRNA by a non-canonical event called back-splicing. They are highly stable, evolutionarily conserved, and wid...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9030659
更新日期：2020-03-09 00:00:00
abstract：:Membrane-bound proteases play a key role in biology by degrading matrix proteins or shedding adhesion receptors. MT1-MMP metalloproteinase is critical during cancer invasion, angiogenesis, and development. MT1-MMP activity is strictly regulated by internalization, recycling, autoprocessing but also through its incorpo...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9020348
更新日期：2020-02-03 00:00:00
abstract：:The function and regulation of amyloid-beta (Aβ) in healthy and diseased liver remains unexplored. Because Aβ reduces the integrity of the blood-brain barrier we have examined its potential role in regulating the sinusoidal permeability of normal and cirrhotic liver. Aβ and key proteins that generate (beta-secretase 1...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9020452
更新日期：2020-02-17 00:00:00
abstract：:The primary cilium is an organelle involved in cellular signalling. Mutations affecting proteins involved in cilia assembly or function result in diseases known as ciliopathies, which cause a wide variety of phenotypes across multiple tissues. These mutations disrupt various cellular processes, including regulation of...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9020278
更新日期：2020-01-23 00:00:00
abstract：:Tubulin is a heterodimer of α and β subunits, both existing as isotypes differing in amino acid sequence encoded by different genes. Specific isotypes of tubulin have associations with cancer that are not well understood. Previous studies found that βII-tubulin is expressed in a number of transformed cells and that th...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8010025
更新日期：2019-01-07 00:00:00
abstract：:We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties. The differentiati...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9030682
更新日期：2020-03-11 00:00:00
