Abstract:
:While high levels of saturated fatty acids are associated with impairment of cardiovascular functions, n-3 polyunsaturated fatty acids (PUFAs) have been shown to exert protective effects. However the molecular mechanisms underlying this evidence are not completely understood. In the present study we have used rat H9c2 ventricular cardiomyoblasts as a cellular model of lipotoxicity to highlight the effects of palmitate, a saturated fatty acid, on genetic and epigenetic modulation of fatty acid metabolism and fate, and the ability of PUFAs, eicosapentaenoic acid, and docosahexaenoic acid, to contrast the actions that may contribute to cardiac dysfunction and remodeling. Treatment with a high dose of palmitate provoked mitochondrial depolarization, apoptosis, and hypertrophy of cardiomyoblasts. Palmitate also enhanced the mRNA levels of sterol regulatory element-binding proteins (SREBPs), a family of master transcription factors for lipogenesis, and it favored the expression of genes encoding key enzymes that metabolically activate palmitate and commit it to biosynthetic pathways. Moreover, miR-33a, a highly conserved microRNA embedded in an intronic sequence of the SREBP2 gene, was co-expressed with the SREBP2 messenger, while its target carnitine palmitoyltransferase-1b was down-regulated. Manipulation of the levels of miR-33a and SREBPs allowed us to understand their involvement in cell death and hypertrophy. The simultaneous addition of PUFAs prevented the effects of palmitate and protected H9c2 cells. These results may have implications for the control of cardiac metabolism and dysfunction, particularly in relation to dietary habits and the quality of fatty acid intake.
journal_name
Cellsjournal_title
Cellsauthors
Cetrullo S,D'Adamo S,Panichi V,Borzì RM,Pignatti C,Flamigni Fdoi
10.3390/cells9030537subject
Has Abstractpub_date
2020-02-26 00:00:00issue
3issn
2073-4409pii
cells9030537journal_volume
9pub_type
杂志文章相关文献
Cells文献大全abstract::Actin remodeling is frequently regulated by antagonistic activities driving protrusion and contraction downstream of Rac and Rho small GTPases, respectively. WAVE regulatory complex (WRC), which primarily operates downstream of Rac, plays pivotal roles in neuronal morphogenesis. Recently, two independent studies descr...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9061355
更新日期:2020-05-29 00:00:00
abstract::Background: Mutations in bone morphogenetic protein receptor type II (BMPR2) are leading to the development of hereditary pulmonary arterial hypertension (PAH). In non-hereditary forms of PAH, perturbations in the transforming growth factor-β (TGF-β)/BMP-axis are believed to cause deficient BMPR2 signaling by changes ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9061422
更新日期:2020-06-08 00:00:00
abstract::Transient receptor potential classical or canonical 4 (TRPC4) and TRPC5 channels are members of the classical or canonical transient receptor potential (TRPC) channel family of non-selective cation channels. TRPC4 and TRPC5 channels are widely accepted as receptor-operated cation channels that are activated in a phosp...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells7110218
更新日期:2018-11-20 00:00:00
abstract::All eukaryotes rely on the ubiquitin-proteasome system (UPS) and autophagy to control the abundance of key regulatory proteins and maintain a healthy intracellular environment. In the UPS, damaged or superfluous proteins are ubiquitinated and degraded in the proteasome, mediated by three types of ubiquitin enzymes: E1...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9102219
更新日期:2020-10-01 00:00:00
abstract::Background: In retinitis pigmentosa (RP), inherited rod death is followed by cone loss and blindness. Why cones die is still a matter of consideration. Here, we investigate the pathogenic role of the sympathetic transmission in the rd10 mouse model of RP. Methods: Retinal levels of beta adrenergic receptor (BAR) 2 and...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9092060
更新日期:2020-09-09 00:00:00
abstract::Transcriptional coactivator with a PDZ-binding motif (TAZ) is one of the mammalian orthologs of Drosophila Yorkie, a transcriptional coactivator of the Hippo pathway. TAZ has been suggested to function as a regulator that modulates the expression of cell proliferation and anti-apoptotic genes in order to stimulate cel...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9010171
更新日期:2020-01-09 00:00:00
abstract::The aim of this review is to outline emerging biomarkers that can serve as early diagnostic tools to identify patients with nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) and, among them, the subgroup of best candidates for clinical trials on emerging compounds. Regarding possible pre...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9041005
更新日期:2020-04-17 00:00:00
abstract::The Rho GTPases comprise a subfamily of the Ras superfamily of small GTPases. Their importance in regulation of cell morphology and cell migration is well characterized. According to the prevailing paradigm, Cdc42 regulates the formation of filopodia, Rac1 regulates the formation of lamellipodia, and RhoA triggers the...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8070759
更新日期:2019-07-21 00:00:00
abstract::Oral cavity cancer is the most frequent malignancy of the head and neck. Unfortunately, despite educational interventions for prevention and early diagnosis, oral cancer patients are often diagnosed in advanced stages associated with poor prognosis and life expectancy. Therefore, there is an urgent need to find noninv...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells8121653
更新日期:2019-12-17 00:00:00
abstract::As sessile organisms, plants must adjust their growth to withstand several environmental conditions. The root is a crucial organ for plant survival as it is responsible for water and nutrient acquisition from the soil and has high phenotypic plasticity in response to a lack or excess of them. How plants sense and tran...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9122576
更新日期:2020-12-01 00:00:00
abstract::Rac1 is a small GTPase that belongs to the Rho family. The Rho family of small GTPases is a subfamily of the Ras superfamily. The Rho family of GTPases mediate a plethora of cellular effects, including regulation of cytoarchitecture, cell size, cell adhesion, cell polarity, cell motility, proliferation, apoptosis/surv...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells7120263
更新日期:2018-12-11 00:00:00
abstract::Mitochondrial dysfunction and iron (Fe) dyshomeostasis are invoked among the mechanisms contributing to muscle aging, possibly via a detrimental mitochondrial-iron feed-forward loop. We quantified the labile Fe pool, Fe isotopes, and the expression of mitochondrial Fe handling proteins in muscle biopsies obtained from...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9122579
更新日期:2020-12-02 00:00:00
abstract::The pathogenesis of inflammatory bowel disease (IBD) is not well-understood; however, increased and persistent intestinal inflammation, due to inappropriate immune responses that are caused by interactions between genetic factors, gut microbiota, and environmental factors, are thought to lead to IBD. Various studies h...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells8050404
更新日期:2019-05-01 00:00:00
abstract::Triple-negative breast cancer (TNBC) is an aggressive breast tumor subtype that currently lacks targeted treatment options. The role played by the insulin-like growth factor-1 (IGF-1) and its cognate receptor IGF-1R in TNBC has been reported. Nevertheless, the molecular mechanisms by which the IGF-1/IGF-1R system may ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9041010
更新日期:2020-04-18 00:00:00
abstract::Macroautophagy (hereafter referred to as autophagy, a word derived from Greek meaning "auto-digestion") is a lysosome-dependent quality control process to degrade and turnover damaged or senescent organelles and proteins for cellular renewal [...]. ...
journal_title:Cells
pub_type: 社论
doi:10.3390/cells8070743
更新日期:2019-07-19 00:00:00
abstract::The plant circadian clock controls a large number of internal processes, including growth and metabolism. Scent emission displays a circadian pattern in many species such as the snapdragon. Here we show that knocking down LATE ELONGATED HYPOCOTYL in Antirrhinum majus affects growth and scent emission. In order to gain...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8080920
更新日期:2019-08-17 00:00:00
abstract::Through the combined action of ubiquitinating and deubiquitinating enzymes, conjugation of ubiquitin to a target protein acts as a reversible post-translational modification functionally similar to phosphorylation. Indeed, ubiquitination is more and more recognized as a central process for the fine regulation of many ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells3020386
更新日期:2014-05-06 00:00:00
abstract::Although G protein-coupled receptor kinases (GRKs) have long been known to regulate G protein-coupled receptor (GPCR) desensitization, their more recently characterized functions as scaffolds and signalling adapters underscore that this small family of proteins governs a larger array of physiological functions than or...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells10010075
更新日期:2021-01-05 00:00:00
abstract::Metastatic spread is mainly sustained by cancer stem cells (CSC), a subpopulation of cancer cells that displays stemness features. CSC are thought to be derived from cancer cells that undergo epithelial to mesenchymal transition (EMT), thus acquiring resistance to anoikis and anti-cancer drugs. After detachment from t...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8111434
更新日期:2019-11-13 00:00:00
abstract::The turnip Brassica rapa has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are targets of CULLIN3-bas...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8040336
更新日期:2019-04-10 00:00:00
abstract::Glioblastoma (GBM) is one of the most intransigent and aggressive brain tumors, and its treatment is extremely challenging and ineffective. To improve patients' expectancy and quality of life, new therapeutic approaches were investigated. Melatonin is an endogenous indoleamine with an incredible variety of properties....
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9030599
更新日期:2020-03-03 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPARs), members of the nuclear hormone receptor family, attract wide attention as promising therapeutic targets for the treatment of multiple diseases, and their target selective ligands were also intensively developed for pharmacological agents such as the approved drugs f...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9020352
更新日期:2020-02-03 00:00:00
abstract::Notch receptors are single-pass transmembrane proteins that play a critical role in cell fate decisions and have been implicated in the regulation of many developmental processes. The human Notch family comprises of four receptors (Notch 1 to 4) and five ligands. Their signaling can regulate extremely basic cellular p...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells10010016
更新日期:2020-12-24 00:00:00
abstract::Non-human primates (NHP) are important surrogate models for late preclinical development of advanced therapy medicinal products (ATMPs), including induced pluripotent stem cell (iPSC)-based therapies, which are also under development for heart failure repair. For effective heart repair by remuscularization, large numb...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9061349
更新日期:2020-05-29 00:00:00
abstract::The mitochondrial genetic disorder, Leber's hereditary optic neuropathy (LHON), is caused by a mutation in MT-ND4 gene, encoding NADH dehydrogenase subunit 4. It leads to the progressive death of retinal ganglion cells (RGCs) and causes visual impairment or even blindness. However, the precise mechanisms of LHON disea...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8060625
更新日期:2019-06-21 00:00:00
abstract::The pathological hallmark of Parkinson's disease (PD) is the loss of neuromelanin-containing dopaminergic neurons within the substantia nigra pars compacta (SNpc). Additionally, numerous studies indicate an altered synaptic function during disease progression. To gain new insights into the molecular processes underlyi...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9122580
更新日期:2020-12-02 00:00:00
abstract::Aquaporins are membrane channels in the broad family of major intrinsic proteins (MIPs), with 13 classes showing tissue-specific distributions in humans. As key physiological modulators of water and solute homeostasis, mutations, and dysfunctions involving aquaporins have been associated with pathologies in all major ...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells10020215
更新日期:2021-01-22 00:00:00
abstract::Tumor recurrence after radiotherapy due to the presence of breast cancer stem cells (BCSCs) is a clinical challenge, and the mechanism remains unclear. Low levels of ROS and enhanced antioxidant defenses are shown to contribute to increasing radioresistance. However, the role of Nrf2-Keap1-Bach1 signaling in the radio...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells10010083
更新日期:2021-01-06 00:00:00
abstract::Anti-Müllerian hormone (AMH) is responsible for the Müllerian ducts' regression in male fetuses. In cells of cancers with AMH receptors (AMHRII), AMH induces cell cycle arrest or apoptosis. As AMH occurs naturally and does not exhibit significant side effects while reducing neoplastic cell colonies, it can be consider...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9102312
更新日期:2020-10-17 00:00:00
abstract::For decades, cardiovascular disease (CVD) has been the leading cause of death throughout most developed countries. Several studies relate RNA splicing, and more recently also circular RNAs (circRNAs), to CVD. CircRNAs originate from linear transcripts and have been shown to exhibit tissue-specific expression profiles....
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9071616
更新日期:2020-07-04 00:00:00