Abstract：
:Sampling and drug stability in oral fluid (OF) are crucial factors when interpreting forensic toxicological analysis, mainly because samples may not be analyzed immediately after collection, potentially altering drug concentrations. Therefore, the stability of some common drugs of abuse (morphine, codeine, 6-monoacetylmorphine, cocaine, benzoylecgonine, Δ9-tetrahydrocannabinol, cannabidiol, amphetamine, 3,4-methylenedioxymethamphetamine, ketamine) and the more commonly consumed new psychoactive substances in our environment (mephedrone, and N-(adamantan-1-yl)-1-(5-fluoropentyl)-1H-indazole-3-carboxamide 5F-AKB48 also known as 5F-APINACA) was investigated in an OF pool for the presence and absence of M3 Reagent Buffer® up to 1 year of storage. Fortified OF samples were stored at three different temperatures (room temperature, 4 and -20°C) to determine the best storage conditions over time. Control fortified OF samples were stored at -80°C for reference purposes. Compounds with concentration changes within ±15% of initial value were considered stable. The drugs were significantly more stable in M3 Reagent Buffer® than in neat OF samples in all storage conditions. All analytes were stable for 1 year at 4°C and -20°C in M3 Reagent Buffer®. Drugs stability in OF varied depending on the analyte, the presence of a stabilizer, the storage duration and temperature. When immediate sample analysis is not possible, we suggest to store OF samples at 4 or -20°C and test them within 2 weeks. Alternatively, OF samples may be stored at 4 or -20°C with M3 Reagent Buffer® to be tested within 1 year.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Marchei E,Malaca S,Graziano S,Gottardi M,Pichini S,Busardò FPdoi
10.1093/jat/bkz114subject
Has Abstractpub_date
2020-07-31 00:00:00pages
570-579issue
6eissn
0146-4760issn
1945-2403pii
5722391journal_volume
44pub_type
杂志文章abstract：:A hair screening method has been developed for the detection of methamphetamine using an immunoassay analyzer (AxSYM) with a fluorescence polarization immunoassay (FPIA) technique. The method consisted of washing, cutting and digesting a hair sample (5 mg) with an enzymatic digestion solution. The digested hair sample...
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doi：10.1093/jat/13.5.296
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doi：10.1093/jat/8.5.244
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pub_type： 杂志文章
doi：10.1093/jat/31.6.342
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pub_type： 杂志文章
doi：10.1093/jat/16.4.261
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pub_type： 杂志文章
doi：10.1093/jat/6.2.64
更新日期：1982-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1093/jat/31.4.181
更新日期：2007-05-01 00:00:00
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journal_title：Journal of analytical toxicology
pub_type： 杂志文章,评审
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更新日期：2017-04-01 00:00:00
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journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/20.3.185
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pub_type： 杂志文章
doi：10.1093/jat/17.7.436
更新日期：1993-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1093/jat/7.1.11
更新日期：1983-01-01 00:00:00
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journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/11.5.210
更新日期：1987-09-01 00:00:00
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journal_title：Journal of analytical toxicology
pub_type： 临床试验,杂志文章
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更新日期：1990-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1980-11-01 00:00:00
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doi：10.1093/jat/11.6.237
更新日期：1987-11-01 00:00:00
