Abstract:
:The classical definitions of method detection limit (MDL) and limit of quantitation (LOQ) are redefined using calibration curve regression analysis. Traditional ways of determining these parameters provide scant information on the daily precision of the analysis at all concentration levels. These parameters are time consuming to acquire and hold only for the moment of acquisition. It is illustrated, with experimental data, using isotope dilution analyses of THC-COOH, a marijuana metabolite, that not only can MDL and LOQ be obtained directly from the calibration curve, but confidence intervals for the calibration curve can also be obtained, which define the precision of the analyses at all levels calibrated. If calibration and analyses of unknowns take place simultaneously, then an MDL and confidence intervals that are relevant to data acquisition are obtained. Confidence intervals at particular analyte concentrations of interest were of greater value than the MDL and LOQ in evaluation of the analytical method. The parameter from the calibration curve is defined as the calibrated quantitation limit.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Schep RAdoi
10.1093/jat/14.5.296subject
Has Abstractpub_date
1990-09-01 00:00:00pages
296-300issue
5eissn
0146-4760issn
1945-2403journal_volume
14pub_type
杂志文章abstract::The List of Prohibited Substances and Methods (the List), an International Standard published yearly by the World Anti-Doping Agency (WADA), determines which substances and methods are prohibited in sport in- and out-of-competition. Stimulants are included within drug class S.6 under the in-competition testing sectio...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/anatox/35.9.613
更新日期:2011-11-01 00:00:00
abstract::Metabolism experiments were conducted with rats dosed with nine azo dyes based on dimethyl-, dimethoxy-, or dichlorobenzidine to determine whether the free amine congeners, their monoacetyl or diacetyl metabolites, or alkaline hydrolyzable conjugates were excreted in the urine. After preliminary tests of the dyes, 2-m...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/7.1.55
更新日期:1983-01-01 00:00:00
abstract::Feces are a possible medium to be used for horse doping control. Efficient methods for detecting drugs in feces collected from various animals are routinely applied in institutes of food safety in Belgium. We have already tested whether they are applicable to horse feces. In this report, accelerated solvent extraction...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/30.5.323
更新日期:2006-06-01 00:00:00
abstract::The quantity of thiocyanate ion in vegetables has been determined due to its relationship with endemic goiter. The technique is based on the use of gas chromatography with electron capture detection (ECD), which provides good sensitivities. ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/12.6.307
更新日期:1988-11-01 00:00:00
abstract::An automated column-switching liquid chromatographic-atmospheric pressure chemical ionization-mass spectrometric (LC-APCI-MS) method for the simultaneous determination of frequently encountered benzodiazepine hypnotics and their relevant metabolites in urine has been established. A column-switching online solid-phase ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/26.2.87
更新日期:2002-03-01 00:00:00
abstract::A rapid, simple, highly sensitive procedure for the simultaneous analysis of bromazepam, alprazolam, clonazepam, lorazepam, oxazepam, diazepam, midazolam, flurazepam, flunitrazepam, nordiazepam, triazolam, temazepam, nitrazepam, and chlordiazepoxide in oral fluid is described, using liquid chromatography coupled to a ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/31.9.596
更新日期:2007-11-01 00:00:00
abstract::A sensitive and specific gas chromatography/mass spectrometry (GC/MS) method has been developed for the simultaneous analysis of methampthetamine and its metabolites (amphetamine, p-hydroxymethamphetamine, and p-hydroxyamphetamine) in monkey urine. The method has been applied to the analysis of urine samples from monk...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/13.2.117
更新日期:1989-03-01 00:00:00
abstract::The content, chemical form, and distribution of mercury and selenium in edible tissue were determined for several samples of fish and other marine animal organisms (mollusks, crustaceans and pods). For most fish samples, except Pacific blue marlin, 53 to 94% of the total mercury content was present as methylmercury, b...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/6.1.10
更新日期:1982-01-01 00:00:00
abstract::This article reports the impact of diuresis on urinary excretion of ethanol in seven healthy volunteers who drank 1000 mL of export beer (44 g ethanol) in 30 min and, 120 min later, ingested 500 or 1000 mL of water within 5 min. Urine was voided before drinking started and every 30-60 min for 360 min after the start o...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/23.7.565
更新日期:1999-11-01 00:00:00
abstract::A high-performance liquid chromatographic system based on gradient elution of an alumina packing material coated with polybutadiene is presented. The chromatographic eluent is a mixture of methanol and water, both containing 0.0125M NaOH. The effluent is monitored by photodiode array detection. The system allows detec...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/19.2.73
更新日期:1995-03-01 00:00:00
abstract::The method presented describes a sensitive and specific quantitative assay for the determination of flumazenil in human plasma. Flumazenil and an internal standard, midazolam, are isolated by a basic extraction. The final extract is separated on a 25-m BP-1 capillary column and drugs are detected by selected ion monit...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/15.4.202
更新日期:1991-07-01 00:00:00
abstract::Postmortem redistribution (PMR) constitutes a multifaceted process, which complicates the interpretation of drug concentrations by forensic toxicologists. The present study aimed to apply quantitative structure-activity relationship (QSAR) analysis for modeling PMR data of structurally related drugs, 10 benzodiazepine...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bku025
更新日期:2014-06-01 00:00:00
abstract::Part I. Expert 4: The object of this study was to investigate the applicability of commercially available expert system shells to interpretation in forensic toxicology. Amitriptyline toxicology was selected as a pilot trial. Blood and tissue concentrations of amitriptyline and nortriptyline in fatal and nonfatal amitr...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/12.4.216
更新日期:1988-07-01 00:00:00
abstract::Doxepin is a tricyclic antidepressant that is widely prescribed for the treatment of mild depression. In this study, hair samples collected from a patient receiving 25 mg of doxepin daily were analyzed. Doxepin was administered to the patient for 4 months (June 15 to October 15, 1996). Five hair samples were collected...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/22.6.531
更新日期:1998-10-01 00:00:00
abstract::Oral fluid (OF) is increasingly used for clinical, forensic and workplace drug testing as an alternative to urine. Uncertainties surrounding OF collection device performance, drug stability and testing reproducibility may be partially responsible for delays in the implementation of OF testing in regulated drug testing...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkv093
更新日期:2015-10-01 00:00:00
abstract::The Substance Abuse and Mental Health Services Administration (SAMHSA) is currently evaluating hydrocodone (HC) for inclusion in the Mandatory Guidelines for Federal Workplace Drug Testing Programs. This study evaluated the time course of HC, norhydrocodone (NHC), dihydrocodeine (DHC) and hydromorphone (HM) in paired ...
journal_title:Journal of analytical toxicology
pub_type: 临床试验,杂志文章
doi:10.1093/jat/bkv050
更新日期:2015-09-01 00:00:00
abstract::Dried blood spots (DBS) on filter paper have been used in human medicine since the 1960s, predominantly for screening in-borne metabolic disorders and more recently, for toxicology. Despite its 50-year existence, this technology has not been adopted by veterinarians for routine diagnoses and research. We have validate...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkt048
更新日期:2013-09-01 00:00:00
abstract::An analytical procedure for the determination of the new pain medication tapentadol and its main metabolite N-desmethyltapentadol (DMT), in urine and oral fluid has been developed and validated using liquid chromatography with tandem mass spectral detection (LC-MS-MS). Oral fluid was collected using Quantisal™ devices...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/34.8.458
更新日期:2010-10-01 00:00:00
abstract::An enzyme multiplied immunoassay technique (EMIT) was used to test for opiates (morphine, hydromorphone, and codeine) in extracts of blood, bile, and tissue homogenates. All immunoassay opiate positive specimens were then tested by a reversed-phase liquid chromatographic procedure using electrochemical detection (LCEC...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/8.2.78
更新日期:1984-03-01 00:00:00
abstract::Synthetic cannabinoids emerged on the designer drug market in recent years due to their ability to produce cannabis-like effects without the risk of detection by traditional drug testing techniques such as immunoassay and gas chromatography-mass spectrometry. As government agencies work to schedule existing synthetic ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkv137
更新日期:2016-04-01 00:00:00
abstract::A comparison was made of several cannabinoid urine assays. Two hundred randomly selected urine specimens were initially screened by two enzyme immunoassays (EMIT-st and EMIT-d.a.u.) and a radioimmunoassay (Abuscreen RIA). Selected specimens found positive by any of these methods were further analyzed by gas-liquid chr...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/8.4.192
更新日期:1984-07-01 00:00:00
abstract::In clinical and forensic toxicology, general unknown screening is used to detect and identify exogenous compounds. In this study, we aimed to develop a fast (15 min) comprehensive screening method for 500 toxicologically relevant analytes based on ultra-performance liquid chromatography (UPLC) coupled with a quadrupol...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/34.9.571
更新日期:2010-11-01 00:00:00
abstract::The measurement of cefoperazone concentrations in cerebral spinal fluid (CSF) is important to understanding the biodisposition and pharmacokinetic behavior of the drug. We have demonstrated that cefoperazone is unstable in methanol and at alkaline pH and these factors may affect the accurate quantitation of the drug i...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/6.2.85
更新日期:1982-03-01 00:00:00
abstract::A relatively simple procedure for the determination of carboxyhemoglobin percent saturation in postmortem samples is described. The procedure involves a quick and accurate method for calibration of the gas chromatograph to carbon monoxide. The mixed gases are extracted from blood prior to introduction into the gas chr...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/4.4.181
更新日期:1980-07-01 00:00:00
abstract::3,4-Methylenedioxymethamphetamine (MDMA), or ecstasy, is excreted as unchanged drug, 3,4-methylenedioxyamphetamine (MDA), and free and glucuronidated/sulfated 4-hydroxy-3-methoxymethamphetamine (HMMA), and 4-hydroxy-3-methoxyamphetamine (HMA) metabolites. The aim of this paper is to describe the pattern and timeframe ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/33.8.439
更新日期:2009-10-01 00:00:00
abstract::Radioactive methadone, phenytoin, phenobarbital, and diazepam were easily and efficiently removed from human urine samples by adsorption on C18 bonded silica phases. These techniques were simpler than conventional extractions at pH 9.3 or 5.2 with dichloromethane, XAD-2 and methanol, or Celite 560 and dichloromethane....
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/9.6.269
更新日期:1985-11-01 00:00:00
abstract::An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method was developed and validated for the simultaneous determination of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-Delta(9)-tetrahydrocannabinol (11-OH-THC), and 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH) in whole blo...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/32.5.349
更新日期:2008-06-01 00:00:00
abstract::Baclofen (Lioresal), a lipophilic analogue of c-aminobutyric acid (GABA), and carisoprodol (Soma), a central nervous system depressant with an unknown mechanism of pharmacologic action, are categorized as muscle relaxants. Baclofen is used clinically in the management of spasticity and its sequelae secondary to severe...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/27.5.275
更新日期:2003-07-01 00:00:00
abstract::A simple, rapid ethanol screen based on a microdiffusion technique (Analytical Systems, Marion Laboratories) has been evaluated and compared to an established enzymatic procedure (Abbott TDx). They showed agreement in 484 of 488 biological specimens and all control specimens. They disagreed on 4 specimens--3 positive ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/12.6.348
更新日期:1988-11-01 00:00:00
abstract::The testing for drugs of abuse in hair is increasingly used to detect illicit substances. Laboratories have implemented various decontamination, or washing, procedures in order to eliminate concerns regarding potential contamination of the hair with drug from the environment. However, the effect of these decontaminati...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/25.7.490
更新日期:2001-10-01 00:00:00