Abstract:
:A high-performance liquid chromatographic system based on gradient elution of an alumina packing material coated with polybutadiene is presented. The chromatographic eluent is a mixture of methanol and water, both containing 0.0125M NaOH. The effluent is monitored by photodiode array detection. The system allows detection and identification of more than 130 toxicologically relevant substances (mainly basic compounds) and has already been successfully applied to screen more than 500 extracts of fresh or postmortem specimens (blood, urine, tissue homogenates, and stomach contents).
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Lambert WE,Meyer E,De Leenheer APdoi
10.1093/jat/19.2.73subject
Has Abstractpub_date
1995-03-01 00:00:00pages
73-8issue
2eissn
0146-4760issn
1945-2403journal_volume
19pub_type
杂志文章abstract::This study evaluated the validity of measuring fentanyl concentrations in equine plasma using radioimmunoassay (RIA) by comparing it to the established technique of liquid chromatography-mass spectrometry (LC-MS). Equine plasma samples were analyzed using a solid-phase Coat-A-Count fentanyl RIA and a validated LC-MS m...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/32.9.754
更新日期:2008-11-01 00:00:00
abstract::A liquid chromatographic method was developed for the analysis of indandione and 4-hydroxycoumarin anticoagulant rodenticides in blood serum and liver. The method enabled the measurement of serum and liver concentrations of eight anticoagulant rodenticides: brodifacoum, bromadiolone, chlorophacinone, coumafuryl, couma...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/17.1.56
更新日期:1993-01-01 00:00:00
abstract::Pharmacogenomics is the study of the linkage between an individual's genotype and the disposition of drugs in the body. The first association between adverse drug reactions and inherited variations was recognized in the 1950s, and since then, pharmacogenomics has come a long way. The importance of pharmacogenomics is ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章,评审
doi:10.1093/jat/30.2.65
更新日期:2006-03-01 00:00:00
abstract::While earlier studies have attempted to resolve the challenges encountered when interpreting gamma-hydroxybutyric acid (GHB) concentrations in hair (primarily due to its endogenous presence), few have had large sample sizes. The first objective of this study was to evaluate the inter-individual variation of endogenous...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkaa080
更新日期:2020-10-12 00:00:00
abstract::3,4-Methylenedioxymethamphetamine (MDMA), a commonly encountered drug of abuse, has been shown in a variety of studies to cause neurotoxic effects. Because MDMA itself is not neurotoxic, identifying the potential neurotoxic metabolite(s) was of significant importance. Evaluation of urine and plasma concentrations of M...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/31.3.138
更新日期:2007-04-01 00:00:00
abstract::There have been numerous reports of lead poisoning resulting from the ingestion of foreign bodies. A case involving the ingestion of spent air rifle pellets is described. No clinical symptoms were observed, despite the fact that the young child exhibited elevated blood lead levels as high as 2.7 micromol/L (56 microg/...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/26.6.370
更新日期:2002-09-01 00:00:00
abstract::During periodate degradation of interfering ephedrine, pseudoephedrine, and phenylpropanolamine in the extraction of methamphetamine from urine, it was observed that a small amount of methamphetamine was demethylated to amphetamine. although all three interfering phenylpropanolamines could be degraded by periodate at ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/18.6.331
更新日期:1994-10-01 00:00:00
abstract::A simple, rapid, and reliable gas-liquid chromatographic (GLC) confirmation procedure was developed for urine samples found positive by the EMIT-dau benzodiazepine metabolite assay. The procedure involves acid hydrolysis, organic extraction, and identification using GLC-nitrogen-phosphorus detection (NPD). The method ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/6.6.305
更新日期:1982-11-01 00:00:00
abstract::This study describes a matrix-assisted laser-desorption-ionization (MALDI) mass spectrometry for rapid screening of 12 diuretics in spiked urine. C60 is used as the matrix for MALDI. Diuretics are directly analyzed by C60-MALDI without previous derivatization. The fact that most diuretic molecules contain sulfate grou...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/23.5.337
更新日期:1999-09-01 00:00:00
abstract::A simple and sensitive method for determination of N,N-dimethyltryptamine (DMT) by gas chromatography (GC) with surface ionization detection (SID) is presented. Whole blood or urine, containing DMT and gramine (internal standard), was subjected to solid-phase extraction with a Sep-Pak C18 cartridge before analysis by ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/21.1.36
更新日期:1997-01-01 00:00:00
abstract::Benzodiazepines are common drugs that cause intoxication. Benzodiazepines and their metabolites can be converted by hydrolysis in acid to the corresponding benzophenones, which are easier to be separated from matrices because of their hydrophobic properties. In this study, a new separation technique called solid-phase...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/23.1.54
更新日期:1999-01-01 00:00:00
abstract::High levels of verapamil (1) were found in the blood, stomach contents, and liver and kidney specimens in a fatal overdose case. The drug was extracted from both acid and enzyme digested tissue with ether, chloroform, and 1-chlorobutane and the digests analysed by both high-performance liquid chromatography and gas ch...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/5.3.105
更新日期:1981-05-01 00:00:00
abstract::Salicylate excretion was studied in the breast milk of a nursing mother (Patient A) taking chronic therapeutic doses of aspirin, and caffeine excretion was monitored in the breast milk of a nursing mother (Patient B) who was a heavy coffee drinker. Salicylate concentrations were maximal in serum at 2.25 hours (10.8 mg...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/6.2.64
更新日期:1982-03-01 00:00:00
abstract::Percutaneous vapor dosing studies have generally used saturated vapor concentration (SVC) measurements to estimate the exposure dose (Ct) of vapor produced from a volatile liquid within a closed system. The purpose of this study was to clarify whether the assumption was valid when translated to a biological system (pi...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/30.3.165
更新日期:2006-04-01 00:00:00
abstract::About 22,000 samples of oral fluid have been collected in five population studies in Norway using either the Intercept or Statsure oral fluid sampling devices. The prevalence of alcohol and drugs was found to be higher in oral fluid samples with small volumes than in those with large volumes for both sampling devices;...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/34.7.416
更新日期:2010-09-01 00:00:00
abstract::Functional magnetic resonance imaging (fMRI), a still-emerging, non-invasive neuroimaging tool, has been applied to a wide range of questions in sensory, motor control, and cognitive psychology. Only more recently has it been applied to understand the sites and mechanisms of action of pharmacological agents within the...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章,评审
doi:10.1093/jat/25.5.419
更新日期:2001-07-01 00:00:00
abstract::3-Fluorophenmetrazine (3-FPM) is a stimulant-like novel psychoactive substance (NPS) and fluorinated analog of phenmetrazine that has recently appeared on the recreational drug market, with limited published information. Likewise, the synthetic opioid U-47700 has gained popularity among recreational drug users and is ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkx060
更新日期:2017-11-01 00:00:00
abstract::A sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the quantitation of oxymorphone (OM) in human whole blood and liver. Sample preparation was done by solid-phase extraction, using deuterated OM as the internal standard. Separation was achieved using a Waters Aquity UPLC ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkt077
更新日期:2013-10-01 00:00:00
abstract::1-(4-Methylphenyl)-2-pyrrolidin-1-ylhexan-1-one (4'-methyl-alpha-pyrrolidinohexanophenone, MPHP) is a new designer drug that appeared on the illicit drug market. It is mainly metabolized to 4'-hydroxymethyl-alpha-pyrrolidinohexanophenone (HO-MPHP) followed by oxidation to the respective carboxylic acid. For studies on...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/33.4.190
更新日期:2009-05-01 00:00:00
abstract::Unknown compounds that were not amenable to GC/MS were found during routine benzodiazepine HPLC screening in a postmortem case. The apparent thermolability made the application of liquid chromatography with mass spectrometry mandatory. The moving-belt interface was used because of its value for identification based on...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/13.1.8
更新日期:1989-01-01 00:00:00
abstract::Analysis and interpretation of the findings for the drug of abuse gamma hydroxybutyric acid (GHB) in fatalities has become very problematic. This is primarily because of variable data in postmortem biological fluids resulting from the endogenous nature of the compound, possible postmortem production, and varying metho...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/28.1.20
更新日期:2004-01-01 00:00:00
abstract::Postmortem femoral blood concentrations of the antipsychotic drugs aripiprazole, chlorprothixene and its metabolite, and quetiapine were determined by LC-MS-MS in 25 cases for aripiprazole and 60 cases each for chlorprothixene and quetiapine. For cases where the cause of death was not related to the considered drugs, ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bku121
更新日期:2015-01-01 00:00:00
abstract::Oral fluid (OF) is increasingly used for clinical, forensic and workplace drug testing as an alternative to urine. Uncertainties surrounding OF collection device performance, drug stability and testing reproducibility may be partially responsible for delays in the implementation of OF testing in regulated drug testing...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkv093
更新日期:2015-10-01 00:00:00
abstract::We developed a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method to measure metabolites of atrazine, phenylurea, and sulfonylurea herbicides in human urine. The metabolites measured in the method include atrazine mercapturate, desethyl atrazine, and desisopropyl atrazine as markers of...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/31.4.181
更新日期:2007-05-01 00:00:00
abstract::This study identified ethyl sulfate (EtS) in human urine and compared the excretion characteristics of EtS with that of ethanol and ethyl glucuronide (EtG). Urine samples were collected from healthy subjects after a single ethanol dose, and also selected from routine clinical samples. Simultaneous analysis of EtS and ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/29.5.270
更新日期:2005-07-01 00:00:00
abstract::For many reasons, forensic toxicologists are being asked to determine and report their measurement uncertainty in blood alcohol analysis. While understood conceptually, the elements and computations involved in determining measurement uncertainty are generally foreign to most forensic toxicologists. Several establishe...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bks012
更新日期:2012-04-01 00:00:00
abstract::Use of methiopropamine (MPA), a synthetic metamfetamine analog, has been detected since 2011 in Europe, but there is limited information on its acute toxicity. A 30-year-old man was admitted to the emergency department in a confused state, with paranoid delusion, auditory and visual hallucinatory experiences, and inco...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkw073
更新日期:2016-11-01 00:00:00
abstract::Sampling and drug stability in oral fluid (OF) are crucial factors when interpreting forensic toxicological analysis, mainly because samples may not be analyzed immediately after collection, potentially altering drug concentrations. Therefore, the stability of some common drugs of abuse (morphine, codeine, 6-monoacety...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkz114
更新日期:2020-07-31 00:00:00
abstract::To determine the degree of ethanol absorption and the resultant formation and urinary excretion of its conjugated metabolites following intensive use of high ethanol content mouthwash, 10 subjects gargled with Listerine(®) antiseptic 4 times daily for 3¼ days. First morning void urine specimens were collected on each ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/anatox/35.5.264
更新日期:2011-06-01 00:00:00
abstract::We describe an assay for measuring cocaine and benzoylecgonine in meconium of infants born to mothers suspected of using cocaine during their pregnancy. The assay involves the use of fluorescence polarization immunoassay (FPIA) to screen for benzoylecgonine in a methanolic extract of the meconium. The FPIA is sensitiv...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/16.4.261
更新日期:1992-07-01 00:00:00