Simultaneous determination of eight anticoagulant rodenticides in blood serum and liver.

Abstract:

:A liquid chromatographic method was developed for the analysis of indandione and 4-hydroxycoumarin anticoagulant rodenticides in blood serum and liver. The method enabled the measurement of serum and liver concentrations of eight anticoagulant rodenticides: brodifacoum, bromadiolone, chlorophacinone, coumafuryl, coumatetralyl, diphacinone, difenacoum, and warfarin. Anticoagulants were extracted from serum and liver with acetonitrile. Extracts were applied to solid-phase extraction columns, which contained mixed packings. Column eluates were evaporated to dryness, reconstituted, and subjected to reversed-phase liquid chromatography. Hydroxycoumarins were detected by fluorescence at an excitation wavelength of 318 nm and an emission wavelength of 390 nm. Indandiones were detected by UV absorption at 285 nm. Extraction efficiencies of greater than 75% for serum and greater than 69% for liver were obtained. The within-run precision (CV) ranged from 2.4 to 8.6% for serum and 2.6 to 8.7% for liver. The between-run precision (CV) ranged from 1.5 to 12.2% for serum and from 2.1 to 11.8% for liver. Hydroxycoumarin rodenticides were detected at 1 ng/mL of serum and 1 ng/g of liver. Indandiones were detected at 10 ng/mL of serum and 10 ng/g of liver.

journal_name

J Anal Toxicol

authors

Chalermchaikit T,Felice LJ,Murphy MJ

doi

10.1093/jat/17.1.56

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

56-61

issue

1

eissn

0146-4760

issn

1945-2403

journal_volume

17

pub_type

杂志文章
  • An online automatic sample cleanup system for the quantitative detection of the benzene exposure biomarker S-phenylmercapturic acid in human urine by electrospray ionization tandem mass spectrometry.

    abstract::An online automatic sample cleanup system was developed for use with electrospray ionization tandem mass spectrometry (ESI-MS-MS) for the quantitative detection of the benzene exposure biomarker S-phenylmercapturic acid (S-PMA) in human urine. The sample clean-up system was constructed with an autosampling device, a r...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/26.4.205

    authors: Liao PC,Li CM,Lin LC,Hung CW,Shih TS

    更新日期:2002-05-01 00:00:00

  • Cocaine Hydroxy Metabolites in Hair: Indicators for Cocaine Use Versus External Contamination☆.

    abstract::Given that external contamination must be considered in hair analysis, there is still a demand for reliable tools to differentiate between incorporation of drugs into the hair as a result of drug consumption and of the hair shaft by external contamination. With the aim of establishing alternative discrimination parame...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/bkz022

    authors: Scholz C,Quednow BB,Herdener M,Kraemer T,Baumgartner MR

    更新日期:2019-08-23 00:00:00

  • Concentration of oxymorphone in postmortem fluids and tissue.

    abstract::Oxymorphone, a semi-synthetic opioid analgesic, has been available as Numorphan since the 1950s in the form of injectable solutions and a suppository. Recently, in 2006, oxymorphone was approved by the Federal Drug Administration for use in the form of immediate and extended release tablets under the trade name Opana....

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/33.3.121

    authors: Garside D,Hargrove RL,Winecker RE

    更新日期:2009-04-01 00:00:00

  • Segmental Analysis of R/S-Methamphetamine and R/S-Amphetamine in Abusers' Head Hair.

    abstract::In this study, the relationships between the concentrations of R/S-methamphetamine (MA) and its metabolite R/S-amphetamine (AP), the AP/MA ratio in hair samples, and MA dependence were investigated by performing segmental hair analysis in MA users. Authentic hair samples collected from 10 chronic MA abusers were cut i...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/bkz089

    authors: Wang T,Shen B,Wu H,Gu J,Shen M,Xiang P

    更新日期:2020-07-31 00:00:00

  • One-step immunoassay for acetaminophen and salicylate in serum, plasma, and whole blood.

    abstract::Acetaminophen (APAP, n-acetyl-p-aminophenol) and salicylate (ASA, acetylsalicylic acid) are two of the most frequently overdosed drugs reported in the emergency room. Most APAP and ASA tests are performed with analytical instruments in laboratories, which are not always ideal for emergency situations. A rapid membrane...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/27.6.366

    authors: Song W,Dou C

    更新日期:2003-09-01 00:00:00

  • Three fatal drug overdoses involving bupropion.

    abstract::Bupropion is a "second generation" antidepressant agent structurally related to the phenethylamines. Postmortem toxicology data are presented from three suicidal drug overdoses in which bupropion was detected. In two cases in which bupropion was the major toxicology finding, peripheral blood levels of the parent drug ...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/17.7.436

    authors: Friel PN,Logan BK,Fligner CL

    更新日期:1993-11-01 00:00:00

  • Simultaneous assay of buprenorphine and norbuprenorphine by negative chemical ionization tandem mass spectrometry.

    abstract::A method for the simultaneous measurement of buprenorphine and its N-dealkylated metabolite, norbuprenorphine, in human plasma was developed with negative chemical ionization tandem mass spectrometry. Buprenorphine and norbuprenorphine were extracted from biological fluids by solid-phase extraction. The samples were d...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/20.4.229

    authors: Kuhlman JJ Jr,Magluilo J Jr,Cone E,Levine B

    更新日期:1996-07-01 00:00:00

  • Bone surface and whole bone as biomarkers for acute fluoride exposure.

    abstract::This study compares fluoride concentrations ([F]) in surface and whole bone for up to 27 days following an acute oral dose of F. Four groups of rats received single oral F dose (50 mg/kg body weight), and the control group received deionized water (n = 10/group). Groups were euthanized at 1, 3, 9, or 27 days after F a...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/29.8.810

    authors: Buzalaf MA,Caroselli EE,de Carvalho JG,de Oliveira RC,da Silva Cardoso VE,Whitford GM

    更新日期:2005-11-01 00:00:00

  • Further evidence for the presence of GHB in postmortem biological fluid: implications for the interpretation of findings.

    abstract::Analysis and interpretation of the findings for the drug of abuse gamma hydroxybutyric acid (GHB) in fatalities has become very problematic. This is primarily because of variable data in postmortem biological fluids resulting from the endogenous nature of the compound, possible postmortem production, and varying metho...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/28.1.20

    authors: Elliott SP

    更新日期:2004-01-01 00:00:00

  • Death by arsenic: a comparative evaluation of exhumed body tissues in the presence of external contamination.

    abstract::External contamination of hair or nails by inorganic arsenic compounds cannot effectively be removed with H2O or dilute HCl. The use of a modified Gutzeit apparatus allowed to establish or rule out external contamination. It was found that contaminated samples will generate large amounts of arsine gas when treated wit...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/7.5.216

    authors: Pirl JN,Townsend GF,Valaitis AK,Grohlich D,Spikes JJ

    更新日期:1983-09-01 00:00:00

  • Field Detection of Drugs of Abuse in Oral Fluid Using the Alere™ DDS®2 Mobile Test System with Confirmation by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).

    abstract::The collection and analysis of drugs in oral fluid (OF) at the roadside has become more feasible with the introduction of portable testing devices such as the Alere™ DDS®2 Mobile Test System (DDS®2). The objective of this study was to compare the on-site results for the DDS®2 to laboratory-based confirmatory assays wi...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/bkx105

    authors: Krotulski AJ,Mohr ALA,Friscia M,Logan BK

    更新日期:2018-04-01 00:00:00

  • Lethal poisoning with ethiofencarb and ethanol.

    abstract::Ethiofencarb is one of the carbamate compounds, which are, in general, less toxic than organophosphorus insecticides. This is due to their reversible acetylcholinesterase inhibition and relative inability to cross the blood-brain barrier. Generally, ethiofencarb is regarded to be of low toxicity (LD(50) > 200 mg/kg); ...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/33.7.389

    authors: Al-Samarraie MS,Karinen R,Rognum T,Hasvold I,Opdal Stokke M,Christophersen AS

    更新日期:2009-09-01 00:00:00

  • Determination of atropine in blood by gas chromatography/mass spectrometry.

    abstract::A gas chromatographic/mass spectrometric (GC/MS) method for the rapid quantitation of atropine (AT) in blood is presented. A 2.0-mL blood specimen containing deuterated N-methyl-atropine as the internal standard was alkaline-hydrolyzed to convert atropine to tropine. The tropine was extracted by organic solvent which ...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/13.5.296

    authors: Saady JJ,Poklis A

    更新日期:1989-09-01 00:00:00

  • Quantitation of diethylene glycol and its metabolites by gas chromatography mass spectrometry or ion chromatography mass spectrometry in rat and human biological samples.

    abstract::The misuse of the commonly used chemical diethylene glycol (DEG) has lead to many poisonings worldwide. Methods were developed for analysis of DEG and its potential metabolites; ethylene glycol, glycolic acid, oxalic acid, diglycolic acid and hydroxyethoxy acetic acid in human urine, serum and cerebrospinal fluid samp...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/bku018

    authors: Perala AW,Filary MJ,Bartels MJ,McMartin KE

    更新日期:2014-05-01 00:00:00

  • Pharmacokinetics of iso-α-acids in volunteers following the consumption of beer.

    abstract::Hop-derived iso-α-acid (IAA) ingredient congeners are specific to beer. Concentrations of IAAs were determined in blood of five volunteers over 6 h following the consumption of small volumes of beer containing relatively high (Pale Ale beer) or low (wheat beer) concentrations of IAAs. IAAs were quickly absorbed with p...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/bku038

    authors: Rodda LN,Gerostamoulos D,Drummer OH

    更新日期:2014-07-01 00:00:00

  • Gas chromatographic determination of urinary o-cresol for the monitoring of toluene exposure.

    abstract::A sensitive and reproducible gas chromatographic procedure for the quantitative determination of urinary o-cresol is described. The first step involves acid hydrolysis (2N HCl, 100 degrees C, 10 min), which yields unconjugated o-cresol. After extraction (methylene chloride, pH 2), the organic layer is concentrated by ...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/20.5.309

    authors: Truchon G,Tardif R,Brodeur J

    更新日期:1996-09-01 00:00:00

  • Analysis of 3,4-methylenedioxymethamphetamine (MDMA) and its metabolites in plasma and urine by HPLC-DAD and GC-MS.

    abstract::In Europe, the compound 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy, Adam), in addition to cannabis, is the most abused illicit drug at all-night "techno" parties. Methods for the determination of MDMA and its metabolites, 4-hydroxy-3-methoxymethamphetamine (HMMA), 3,4-dihydroxy-methamphetamine (HHMA), 3,4-methyl...

    journal_title:Journal of analytical toxicology

    pub_type: 临床试验,杂志文章

    doi:10.1093/jat/20.6.432

    authors: Helmlin HJ,Bracher K,Bourquin D,Vonlanthen D,Brenneisen R

    更新日期:1996-10-01 00:00:00

  • Hair analysis for drugs of abuse: evaluation of analytical methods, environmental issues, and development of reference materials.

    abstract::Methods for extraction of cocaine, some of its metabolites, morphine, and codeine from hair and methods for analyzing the extracts have been investigated. Results of these studies have shown that extractions with 0.1N HCl are efficient at removing the target compounds from hair and appear to be as effective as enzymat...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/17.7.389

    authors: Welch MJ,Sniegoski LT,Allgood CC,Habram M

    更新日期:1993-11-01 00:00:00

  • Chromatographic and spectral properties of some aryl-substituted phencyclidine analogs.

    abstract::As part of a search for phencyclidine (PCP) antagonists, a series of eleven aryl-substituted PCP analogs were prepared and characterized. TLC and GC elution profiles of these derivatives are described in a number of systems allowing for the tentative identification of the PCP analogs. Further, MS, NMR, UV/vis and IR s...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/7.5.252

    authors: Costa JF,Speaker TJ

    更新日期:1983-09-01 00:00:00

  • Selective determination of amitriptyline and nortriptyline in human plasma by HPLC with ultraviolet and particle beam mass spectrometry.

    abstract::A selective, sensitive, and reliable method was devised to determine concentrations of amitriptyline and its major metabolite, nortriptyline, in human plasma using high-performance liquid chromatography (HPLC) combined with UV and particle beam mass spectrometry (PBMS). Amitriptyline and nortriptyline were effectively...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/21.3.185

    authors: Kudo K,Jitsufuchi N,Imamura T

    更新日期:1997-05-01 00:00:00

  • Metabolism and the urinary excretion profile of the recently scheduled designer drug N-Benzylpiperazine (BZP) in the rat.

    abstract::The metabolism of N-benzylpiperazine (BZP), a recently scheduled designer drug, in the rat has been studied by analyzing its urinary metabolites. p-Hydroxy-BZP (p-OH-BZP) was unequivocally identified as the main metabolite along with a minor metabolite m-hydroxy-BZP (m-OH-BZP), using gas chromatography-mass spectromet...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/30.1.38

    authors: Tsutsumi H,Katagi M,Miki A,Shima N,Kamata T,Nakajima K,Inoue H,Kishi T,Tsuchihashi H

    更新日期:2006-01-01 00:00:00

  • Urine concentration Does Not Affect Biochemical Testing for Non-Adherence.

    abstract::Hypertension is one of the most important modifiable risk factor causing cardiovascular disease. Unfortunately, non-adherence to antihypertensive medications is frequently observed in hypertensive patients and can lead to an increase in morbidity and mortality. Until recently, there was no robust clinical method to ob...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/bkaa192

    authors: Burns AD,Alghamadi R,Iqbal A,Davies T,Lane D,Patel P,Gupta P

    更新日期:2020-12-10 00:00:00

  • Bovine blood lead reference material.

    abstract::A protocol is described for the preparation and characterization of bovine blood lead (Pb) reference material (RM). Adult bovine animals are orally dosed with Pb as Pb(NO3)2, and blood is collected directly from the jugular vein into 5-mL evacuated, low-Pb glass tubes containing EDTA as anticoagulant. The blood is coo...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/13.4.204

    authors: Cox DH,Duncan CE,Wyatt GP,Meadow JS,Robinson JB

    更新日期:1989-07-01 00:00:00

  • Plasma determination of flumazenil, a benzodiazepine antagonist, by immunotoxicology and by capillary gas chromatography/mass spectrometry.

    abstract::The method presented describes a sensitive and specific quantitative assay for the determination of flumazenil in human plasma. Flumazenil and an internal standard, midazolam, are isolated by a basic extraction. The final extract is separated on a 25-m BP-1 capillary column and drugs are detected by selected ion monit...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/15.4.202

    authors: Kintz P,Mangin P

    更新日期:1991-07-01 00:00:00

  • High-performance liquid chromatographic-mass spectrometric determination of methamphetamine and amphetamine enantiomers, desmethylselegiline and selegiline, in hair samples of long-term methamphetamine abusers or selegiline users.

    abstract::We devised a highly sensitive method for simultaneously determining methamphetamine (MA) and amphetamine (AP) enantiomers, desmethylselegiline (DMSG) and selegiline (SG), in human hair using a derivatization technique and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). M...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/30.4.232

    authors: Nishida K,Itoh S,Inoue N,Kudo K,Ikeda N

    更新日期:2006-05-01 00:00:00

  • Concentrations of heroin, 06-monoacetylmorphine, and morphine in a lethal case following an oral heroin overdose.

    abstract::A case of lethal overdose by heroin ingestion is presented. The concentrations of drugs were measured several hours after death. Heroin, 06-monoacetylmorphine, and morphine were identified and quantitated in blood, urine, and gastrointestinal contents by gas chromatography-mass spectrometry and high-performance liquid...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/21.3.232

    authors: Rop PP,Fornaris M,Salmon T,Burle J,Bresson M

    更新日期:1997-05-01 00:00:00

  • Determination of heroin, 6-acetylmorphine, and morphine in biological fluids using their propionyl derivatives with ion trap GC-MS.

    abstract::We present an analytical method for the determination of heroin, free 6-acetylmorphine (6-AM), and free morphine in blood, urine, and vitreous humor. This method is used in postmortem cases where heroin is the suspected cause of death. The analytical protocol includes the following steps: liquid-liquid extraction at p...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/21.2.127

    authors: Guillot JG,Lefebvre M,Weber JP

    更新日期:1997-03-01 00:00:00

  • Ethylene glycol and glycolic acid in postmortem blood from fatal poisonings.

    abstract::Ethylene glycol (EG), a relatively infrequent cause of fatal intoxication, presents an analytical challenge for forensic confirmation in postmortem toxicology. We report EG and glycolic acid (GA) quantification in postmortem blood by gas chromatography coupled with ion trap mass spectrometry (GC-MS) analysis using a m...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/33.8.508

    authors: Rosano TG,Swift TA,Kranick CJ,Sikirica M

    更新日期:2009-10-01 00:00:00

  • Validation of analysis of amphetamines, opiates, phencyclidine, cocaine, and benzoylecgonine in oral fluids by liquid chromatography-tandem mass spectrometry.

    abstract::The aim of the present study was to develop and validate a method for the detection and quantitation of drugs of abuse in oral fluids. Fortified oral fluid samples (made in-house) and samples from donors collected with Quantasil oral fluid collection kits from Immunalysis were screened on an Olympus 5400 using reagent...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/32.8.605

    authors: Kala SV,Harris SE,Freijo TD,Gerlich S

    更新日期:2008-10-01 00:00:00

  • Certification of phencyclidine in lyophilized human urine reference materials.

    abstract::The National Institute of Standards and Technology (formerly the National Bureau of Standards), in cooperation with the College of American Pathologists (CAP), has certified the concentrations of phencyclidine (PCP) in two new reference materials (RMs). One of these materials is Standard Reference Material (SRM) 1511,...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章

    doi:10.1093/jat/20.1.43

    authors: Tai SS,Christensen RG,Coakley K,Ellerbe P,Long T,Welch MJ

    更新日期:1996-01-01 00:00:00