Abstract:
:An automated column-switching liquid chromatographic-atmospheric pressure chemical ionization-mass spectrometric (LC-APCI-MS) method for the simultaneous determination of frequently encountered benzodiazepine hypnotics and their relevant metabolites in urine has been established. A column-switching online solid-phase extraction technique was employed to enhance sensitivity and eliminate tedious sample pretreatment. The combination of an N-vinylacetamide-containing hydrophilic polymer online extraction column and a C18 semi-micro LC column provided the successful separations and MS determinations of the 11 benzodiazepines and 11 relevant metabolites tested in this study. The detection limits ranged 2-10 ng/mL and 10-50 ng/mL in the SIM and full-scan modes, respectively, using 50 microL of urine. The calibration curves were linear up to 500 ng/mL for all these analytes in the SIM mode. The present method provided successful forensic applications for the proof of benzodiazepine administration.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Miki A,Tatsuno M,Katagi M,Nishikawa M,Tsuchihashi Hdoi
10.1093/jat/26.2.87keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
87-93issue
2eissn
0146-4760issn
1945-2403journal_volume
26pub_type
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