An online automatic sample cleanup system for the quantitative detection of the benzene exposure biomarker S-phenylmercapturic acid in human urine by electrospray ionization tandem mass spectrometry.


:An online automatic sample cleanup system was developed for use with electrospray ionization tandem mass spectrometry (ESI-MS-MS) for the quantitative detection of the benzene exposure biomarker S-phenylmercapturic acid (S-PMA) in human urine. The sample clean-up system was constructed with an autosampling device, a reversed-phase C18 trap cartridge, a two-position switching valve, and controlling computer software and hardware. The sample cleanup system was interfaced directly with the ESI source of a triple-stage-quadrupole MS using multiple reaction monitoring of negative product ions derived from S-PMA and the internal standard as the detection mode. The calibration curve was linear using human urine spiked at concentrations from 0.23 to 100 mg/L S-PMA (R2 = 0.997). The detection limit of the analytical system for neat S-PMA standard solution was 0.04 microg/L, whereas the detection limit was estimated to be lower than 0.35 microg/L for a urine matrix containing trace amounts of S-PMA. Without tedious manual sample cleanup procedures, the analytical system is fully automatic and therefore useful for high-throughput urinary S-PMA determination. With the selectivity and the sensitivity provided by MS-MS detection, the analytical system can be used for high-throughput and accurate determination of S-PMA levels in human urinary samples as a biomarker for benzene exposure.


J Anal Toxicol


Liao PC,Li CM,Lin LC,Hung CW,Shih TS





Has Abstract


2002-05-01 00:00:00












  • Stability of Synthetic Piperazines in Human Whole Blood.

    abstract::While circumventing legislative controls, synthetic piperazines are encountered as "legal" alternatives to ecstasy. Unforeseeable challenges may delay quantitative analysis of these compounds in biological fluids. Enzymatic reactions, matrix interferences and limited knowledge of analyte stability further complicate i...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Lau T,LeBlanc R,Botch-Jones S

    更新日期:2018-03-01 00:00:00

  • Quantification of five compounds with heterogeneous physicochemical properties (morphine, 6-monoacetylmorphine, cyamemazine, meprobamate and caffeine) in 11 fluids and tissues, using automated solid-phase extraction and gas chromatography-tandem mass spec

    abstract::An automated solid-phase extraction (SPE) protocol followed by gas chromatography coupled with tandem mass spectrometry was developed for quantification of caffeine, cyamemazine, meprobamate, morphine and 6-monoacetylmorphine (6-MAM) in 11 biological matrices [blood, urine, bile, vitreous humor, liver, kidney, lung an...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Bévalot F,Bottinelli C,Cartiser N,Fanton L,Guitton J

    更新日期:2014-06-01 00:00:00

  • An Impaired Driver Found to be Under the Influence of Methoxetamine.

    abstract::The popularity of designer drugs has increased over the past few years as users seek new, cheap and sometimes "legal" ways to get high. This case report focuses on a case that happened in the City of Henderson, Nevada, which involved the designer drug methoxetamine. Methoxetamine is a psychoactive compound that is str...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Fassette T,Martinez A

    更新日期:2016-10-01 00:00:00

  • Separation and quantitation of the enantiomers of methamphetamine and its metabolites in urine by HPLC: precolumn derivatization and fluorescence detection.

    abstract::To study the disposition kinetics of methamphetamine (MAP), we have developed a sensitive high-performance liquid chromatographic (HPLC) assay to quantitate the enantiomers of MAP and its major metabolites, amphetamine (AP), p-hydroxymethamphetamine (p-OH-MAP), and p-hydroxyamphetamine (p-OH-AP), the latter two of whi...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Sukbuntherng J,Hutchaleelaha A,Chow HH,Mayersohn M

    更新日期:1995-05-01 00:00:00

  • Isotopic analogues as internal standards for quantitative analyses of drugs and metabolites by GC-MS--nonlinear calibration approaches.

    abstract::In order to achieve accurate quantitation of drugs and metabolites (analytes) in complex matrices, 2H- (and less commonly 13C-) labeled analogues of the analytes are now routinely adapted as the internal standards (IS) using linear calibration models to fit data generated by selected ion monitoring gas chromatography-...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Whiting TC,Liu RH,Chang WT,Bodapati MR

    更新日期:2001-04-01 00:00:00

  • An HPLC method for the simultaneous quantitation of quinidine, procainamide, N-acetylprocainamide, and disopyramide.

    abstract::A high performance liquid chromatographic method is reported, which incorporates three internal standards (I-cinchonidine, N-propylprocainamide, and para-chlorodisopyramide) for the simultaneous quantitation of four commonly prescribed antiarrhythmic drugs: quinidine, procainamide, N-acetylprocainamide, and disopyrami...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Bridges RR,Jennison TA

    更新日期:1983-03-01 00:00:00

  • Comparison of EMIT II, CEDIA, and DPC RIA assays for the detection of lysergic acid diethylamide in forensic urine samples.

    abstract::In an effort to determine a practical, efficient, and economical alternative for the use of a radioimmunoassay (RIA) for the detection of lysergic acid diethylamide (LSD) in human urine, the performance of two photometric immunoassays (Dade Behring EMIT II and Microgenics CEDIA) and the Diagnostics Products Corp. (DPC...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Wiegand RF,Klette KL,Stout PR,Gehlhausen JM

    更新日期:2002-10-01 00:00:00

  • Suicide due to cyclizine overdose.

    abstract::Cyclizine is an antihistamine with sedative effect used to treat motion sickness. A few studies have reported on cyclizine abuse among teenagers, and cyclizine abuse has been reported among opioid dependants receiving methadone, with the combination having been reported to produce strong psychoactive effects. Few repo...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Arnestad M,Eldor KB,Stray-Pedersen A,Bachs L,Karinen R

    更新日期:2014-03-01 00:00:00

  • Comparison of liquid chromatography-mass spectrometry and radioimmunoassay for measurement of fentanyl and determination of pharmacokinetics in equine plasma.

    abstract::This study evaluated the validity of measuring fentanyl concentrations in equine plasma using radioimmunoassay (RIA) by comparing it to the established technique of liquid chromatography-mass spectrometry (LC-MS). Equine plasma samples were analyzed using a solid-phase Coat-A-Count fentanyl RIA and a validated LC-MS m...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Thomasy SM,Mama KR,Stanley SD

    更新日期:2008-11-01 00:00:00

  • Procedure for the Selection and Validation of a Calibration Model I-Description and Application.

    abstract::Calibration model selection is required for all quantitative methods in toxicology and more broadly in bioanalysis. This typically involves selecting the equation order (quadratic or linear) and weighting factor correctly modelizing the data. A mis-selection of the calibration model will generate lower quality control...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Desharnais B,Camirand-Lemyre F,Mireault P,Skinner CD

    更新日期:2017-05-01 00:00:00

  • Simultaneous determination of monoacetylmorphine, morphine, codeine, and other opiates by GC/MS.

    abstract::A GC/MS method for the simultaneous determination of morphine, codeine, and O-6-monoacetylmorphine as well as other semisynthetic opiates in urine is described. The method employs 2H6-acetic anhydride to acetylate free hydroxyl groups on all of the opiates. As a result, the same hydrolysis and extraction procedure is ...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Bowie LJ,Kirkpatrick PB

    更新日期:1989-11-01 00:00:00

  • Applying quantitative structure-activity relationship (QSAR) methodology for modeling postmortem redistribution of benzodiazepines and tricyclic antidepressants.

    abstract::Postmortem redistribution (PMR) constitutes a multifaceted process, which complicates the interpretation of drug concentrations by forensic toxicologists. The present study aimed to apply quantitative structure-activity relationship (QSAR) analysis for modeling PMR data of structurally related drugs, 10 benzodiazepine...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Giaginis C,Tsantili-Kakoulidou A,Theocharis S

    更新日期:2014-06-01 00:00:00

  • Urine concentrations of fentanyl and norfentanyl during application of Duragesic transdermal patches.

    abstract::A study of the urinary concentration of fentanyl (F) and its major metabolite norfentanyl (NF) in chronic pain patients treated with the Duragesic continuous release transdermal patches is presented. These patches are available in 10, 20, 30, and 40 cm(2) sizes releasing 25, 50, 75, and 100 microg/h F, respectively. F...

    journal_title:Journal of analytical toxicology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Poklis A,Backer R

    更新日期:2004-09-01 00:00:00

  • Screening of basic drugs in biological samples using dual column capillary chromatography and nitrogen-phosphorus detectors.

    abstract::A method is presented for the extraction and screening of basic and neutral drugs, including amphetamines. Two milliliters of blood are buffered to pH 9.0 and extracted with n-chlorobutane. Underivatized extracts are analyzed simultaneously on two complementary capillary columns placed together in one injection port u...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Watts VW,Simonick TF

    更新日期:1986-09-01 00:00:00

  • Drugs of abuse: data collection systems of DEA and recent trends.

    abstract::The U.S. Drug Enforcement Administration has several different systems for collecting information on drugs of abuse. One is an early warning system, which collects information to provide scientific data and interpretation on drug abuse. Another is the collection of data from evidence submitted to DEA laboratories. Thi...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Frank RS

    更新日期:1987-11-01 00:00:00

  • Amphetamine as an artifact of methamphetamine during periodate degradation of interfering ephedrine, pseudoephedrine, and phenylpropanolamine: an improved procedure for accurate quantitation of amphetamines in urine.

    abstract::During periodate degradation of interfering ephedrine, pseudoephedrine, and phenylpropanolamine in the extraction of methamphetamine from urine, it was observed that a small amount of methamphetamine was demethylated to amphetamine. although all three interfering phenylpropanolamines could be degraded by periodate at ...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Paul BD,Past MR,McKinley RM,Foreman JD,McWhorter LK,Snyder JJ

    更新日期:1994-10-01 00:00:00

  • Optimization of storage conditions for cocaine and benzoylecgonine in urine: a review.

    abstract::The maximum stability conditions for cocaine and its metabolite benzoylecgonine (BE) in urine were analyzed. Suggestions were presented after careful review of many studies and personal communications concerning cocaine and BE stability in urine. The optimal storage conditions for urine specimens with cocaine and BE w...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章,评审


    authors: Hippenstiel MJ,Gerson B

    更新日期:1994-03-01 00:00:00

  • Extraction of benzoylecgonine from urine specimens with Cerex Polycrom Clin II solid-phase extraction columns and the Speedisk Pressure Processor.

    abstract::Benzoylecgonine (BZE) extraction from urine was explored using Cerex Polycrom Clin II solid-phase extraction (SPE) columns and the Speedisk 48 Pressure Processor as an alternative to the Prep1 automated sample processor and XTRX Type RP/W columns. Linearity for urine standards extracted using the Cerex-Speedisk method...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Kuhnle JA,Churley M,Kawasaki SY,Lyons TP,Bruins MR

    更新日期:2001-10-01 00:00:00

  • Bovine blood lead reference material.

    abstract::A protocol is described for the preparation and characterization of bovine blood lead (Pb) reference material (RM). Adult bovine animals are orally dosed with Pb as Pb(NO3)2, and blood is collected directly from the jugular vein into 5-mL evacuated, low-Pb glass tubes containing EDTA as anticoagulant. The blood is coo...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Cox DH,Duncan CE,Wyatt GP,Meadow JS,Robinson JB

    更新日期:1989-07-01 00:00:00

  • Toxicokinetics of the Synthetic Cathinone α-Pyrrolidinohexanophenone.

    abstract::Synthetic cathinones inhibit monoamine transporters, such as serotonin, norepinephrine, and dopamine transporters, and act on the central nervous system via increasing synaptic concentrations of monoamines. These compounds, which are highly addictive and potentially poisonous, are new psychoactive substances. In this ...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Fujita Y,Mita T,Usui K,Kamijo Y,Kikuchi S,Onodera M,Fujino Y,Inoue Y

    更新日期:2018-01-01 00:00:00

  • LC-MS-MS Method for Analysis of Opiates in Wastewater During Football Games II.

    abstract::Continuing our previous studies analyzing drugs of abuse in municipal wastewater, a method was developed for the analysis of opiates in wastewater samples using liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). Eight opiate drugs and metabolites were analyzed including codeine, hydrocodone, hydro...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Gul W,Stamper B,Godfrey M,Gul SW,ElSohly MA

    更新日期:2016-06-01 00:00:00

  • Background levels of bromide in human blood.

    abstract::A total of 183 random, whole-blood specimens was collected from healthy individuals within the State of Queensland (Australia), and the bromide concentration was determined by wavelength-dispersive X-ray fluorescence spectrometry (WDXRF). The intensity of the tube Compton scatter line was used to account for differenc...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Olszowy HA,Rossiter J,Hegarty J,Geoghegan P

    更新日期:1998-05-01 00:00:00

  • Determination of atropine in blood by gas chromatography/mass spectrometry.

    abstract::A gas chromatographic/mass spectrometric (GC/MS) method for the rapid quantitation of atropine (AT) in blood is presented. A 2.0-mL blood specimen containing deuterated N-methyl-atropine as the internal standard was alkaline-hydrolyzed to convert atropine to tropine. The tropine was extracted by organic solvent which ...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Saady JJ,Poklis A

    更新日期:1989-09-01 00:00:00

  • Toxicology Laboratory, Harris County Medical Examiner Office, Houston, Texas.

    abstract::A relatively simple procedure for the determination of carboxyhemoglobin percent saturation in postmortem samples is described. The procedure involves a quick and accurate method for calibration of the gas chromatograph to carbon monoxide. The mixed gases are extracted from blood prior to introduction into the gas chr...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Hobbs LD,Jachimczyk JA,Schloegel EL

    更新日期:1980-07-01 00:00:00

  • Determination of blood cyanide by HPLC-MS.

    abstract::An original high-performance liquid chromatographic-mass spectrometric (HPLC-MS) procedure was developed for the determination of cyanide (CN) in whole blood. After the addition of K13C15N as internal standard, blood was placed in a microdiffusion device, the inner well of which was filled with a mixture of taurine (5...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Tracqui A,Raul JS,Géraut A,Berthelon L,Ludes B

    更新日期:2002-04-01 00:00:00

  • Screening of stimulants including designer drugs in urine using a liquid chromatography tandem mass spectrometry system.

    abstract::A rapid, reproducible and sensitive reversed phase liquid chromatography-mass spectrometry method was developed and validated for the identification and semi-quantitative confirmation of stimulants in urine. The method is capable of separating compounds such as cocaine and metabolites, amphetamines, substituted cathin...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: O'Byrne PM,Kavanagh PV,McNamara SM,Stokes SM

    更新日期:2013-03-01 00:00:00

  • The production of amitriptyline from nortriptyline in formaldehyde-containing solutions.

    abstract::The stability of nortriptyline in aqueous solutions containing various concentrations of formaldehyde was investigated. Amitriptyline, as a reaction product, was determined by gas chromatography/mass spectrometry (GC/MS) in these experiments. Factors that may contribute to this phenomenon, including pH, formaldehyde c...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Dettling RJ,Briglia EJ,Dal Cortivo LA,Bidanset JH

    更新日期:1990-09-01 00:00:00

  • Concentrations of heroin, 06-monoacetylmorphine, and morphine in a lethal case following an oral heroin overdose.

    abstract::A case of lethal overdose by heroin ingestion is presented. The concentrations of drugs were measured several hours after death. Heroin, 06-monoacetylmorphine, and morphine were identified and quantitated in blood, urine, and gastrointestinal contents by gas chromatography-mass spectrometry and high-performance liquid...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Rop PP,Fornaris M,Salmon T,Burle J,Bresson M

    更新日期:1997-05-01 00:00:00

  • Direct determination of ethyl glucuronide and ethyl sulfate in postmortem urine specimens using hydrophilic interaction liquid chromatography-electrospray ionization-tandem mass spectrometry.

    abstract::This work was aimed at developing and validating a hydrophilic interaction chromatography (HILIC)-electrospray ionization (ESI)-ion trap-tandem mass spectrometric method for identification and quantification of ethyl glucuronide (ETG) and ethyl sulfate (ETS) as ethanol biomarkers and at employing this method for analy...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Al-Asmari AI,Anderson RA,Appelblad P

    更新日期:2010-06-01 00:00:00

  • Determination of phenanthrene and hydroxyphenanthrenes in various biological matrices at trace levels using gas chromatography-mass spectrometry.

    abstract::A fast and efficient multiresidue extraction-purification procedure was developed for low levels (ppb range) of phenanthrene and hydroxyphenanthrene in biological matrices, in order to quantify phenanthrene and metabolites in blood, milk, urine, and biological tissues of lactating goats. Detection and identification o...

    journal_title:Journal of analytical toxicology

    pub_type: 杂志文章


    authors: Grova N,Monteau F,Le Bizec B,Feidt C,Andre F,Rychen G

    更新日期:2005-04-01 00:00:00