当前位置： SCI文献检索 > JOURNAL OF ANALYTICAL TOXICOLOGY期刊下所有文献 > Determination of L-ephedrine, pseudoephedrine, and caffeine in rat plasma by liquid chromatography-tandem mass spectrometry.

Determination of L-ephedrine, pseudoephedrine, and caffeine in rat plasma by liquid chromatography-tandem mass spectrometry.

Abstract：

:A rapid and simple liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of L-ephedrine, pseudoephedrine, and caffeine in male Fisher-344 rat plasma at nanogram-per-milliliter concentrations for use in support of toxicology studies. Only 25 μL of plasma is required, and extraction is performed using a simple, single-step protein precipitation. The method was validated over a range of 2.09 to 5460 ng/mL for L-ephedrine, 2.09 to 5050 ng/mL for pseudoephedrine and 2.03 to 5340 ng/mL for caffeine. A binary gradient elution at 0.3 mL/min was used with a Waters XBridge Phenyl (2.1 × 150 mm, 3.5 μm) column and a Waters XBridge Phenyl 2.1- × 10-mm guard column at ambient temperature. The mobile phase consisted of 10 mM ammonium acetate in water (pH 5.0) and methanol. Caffeine trimethyl-(13)C(3) was used as the internal standard. The method was evaluated for linearity, recovery, precision, accuracy, and stability, and it was successfully applied in toxicokinetic studies of ephedrine, administered alone, in combination with caffeine, and in the herbal source Ma Huang.

journal_name

J Anal Toxicol

journal_title

Journal of analytical toxicology

authors

Cooper SD,Fletcher BL,Silinski MA,Brown SS,Lodge JW,Fernando RA,Collins BJ

doi

10.1093/anatox/35.6.341

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

341-8

issue

6

eissn

0146-4760

issn

1945-2403

journal_volume

35

pub_type

杂志文章

相关文献

JOURNAL OF ANALYTICAL TOXICOLOGY文献大全
  • The Lethal Cutting: An Unexpected Cause of Death.

    abstract：:Cocaine is among the illicit substances most frequently implicated in deaths related to the use of drugs of abuse both worldwide and in Italy. Cutting agents involved in the adulterations of this substance are many and the process of lacing can take place at various stages of the production of the drug. In this Report...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bkab006

    authors： Di Candia D,Boracchi M,Muccino E,Gentile G,Zoja R

    更新日期：2021-01-08 00:00:00

  • An HPLC method for the simultaneous quantitation of quinidine, procainamide, N-acetylprocainamide, and disopyramide.

    abstract：:A high performance liquid chromatographic method is reported, which incorporates three internal standards (I-cinchonidine, N-propylprocainamide, and para-chlorodisopyramide) for the simultaneous quantitation of four commonly prescribed antiarrhythmic drugs: quinidine, procainamide, N-acetylprocainamide, and disopyrami...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/7.2.65

    authors： Bridges RR,Jennison TA

    更新日期：1983-03-01 00:00:00

  • Quantitation of barbiturates by a modification of the EMIT-tox serum barbiturate assay.

    abstract：:A modification of the Syva EMIT-tox Serum Barbiturate Assay was developed to provide a rapid and practical method for quantitating serum barbiturates. Standard curves for eight selected barbiturates were run using specific sets of standards for each. Correlation coefficients, slopes, and y-intercepts for the curves of...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/7.6.257

    authors： Watson AT,Manno JE,Manno BR

    更新日期：1983-11-01 00:00:00

  • The production of amitriptyline from nortriptyline in formaldehyde-containing solutions.

    abstract：:The stability of nortriptyline in aqueous solutions containing various concentrations of formaldehyde was investigated. Amitriptyline, as a reaction product, was determined by gas chromatography/mass spectrometry (GC/MS) in these experiments. Factors that may contribute to this phenomenon, including pH, formaldehyde c...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/14.5.325

    authors： Dettling RJ,Briglia EJ,Dal Cortivo LA,Bidanset JH

    更新日期：1990-09-01 00:00:00

  • Excretion of cannabinoids in urine after ingestion of cannabis seed oil.

    abstract：:Gas chromatographic-mass spectrometric (GC-MS) quantitation of 25 cannabis sed oils determined delta 9-tetrahydrocannabinol (THC) concentrations from 3 to 1500 micrograms/g oil. In a pilot study, the morning urine of six volunteers who had ingested 11 or 22 g of the oil, which contained the highest THC content (1500 m...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/21.5.373

    authors： Lehmann T,Sager F,Brenneisen R

    更新日期：1997-09-01 00:00:00

  • A simple and sensitive quantitation of N,N-dimethyltryptamine by gas chromatography with surface ionization detection.

    abstract：:A simple and sensitive method for determination of N,N-dimethyltryptamine (DMT) by gas chromatography (GC) with surface ionization detection (SID) is presented. Whole blood or urine, containing DMT and gramine (internal standard), was subjected to solid-phase extraction with a Sep-Pak C18 cartridge before analysis by ...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/21.1.36

    authors： Ishii A,Seno H,Suzuki O,Hattori H,Kumazawa T

    更新日期：1997-01-01 00:00:00

  • Screening, identification, and quantitation of benzodiazepines in postmortem samples by HPLC with photodiode array detection.

    abstract：:A high-performance liquid chromatographic method has been developed for the simultaneous analysis of eight frequently used benzodiazepines and some of their metabolites in postmortem samples. The method includes a liquid-liquid extraction with n-hexane-ethyl acetate (70:30, v/v), gradient elution of a C8 reversed-phas...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/19.1.35

    authors： Lambert WE,Meyer E,Xue-Ping Y,De Leenheer AP

    更新日期：1995-01-01 00:00:00

  • Analysis of codeine positivity in urine of pain management patients.

    abstract：:The opioids codeine and morphine have legitimate uses in managing chronic pain conditions, but they are frequently abused. Patients prescribed opioids submit urine samples for medication compliance monitoring, and the interpretation of the results is complex. The purpose of this study was to evaluate the percentage of...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bkv031

    authors： Colby JM,Wu AH,Lynch KL

    更新日期：2015-06-01 00:00:00

  • Isotopic analogues as internal standards for quantitative analyses of drugs and metabolites by GC-MS--nonlinear calibration approaches.

    abstract：:In order to achieve accurate quantitation of drugs and metabolites (analytes) in complex matrices, 2H- (and less commonly 13C-) labeled analogues of the analytes are now routinely adapted as the internal standards (IS) using linear calibration models to fit data generated by selected ion monitoring gas chromatography-...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/25.3.179

    authors： Whiting TC,Liu RH,Chang WT,Bodapati MR

    更新日期：2001-04-01 00:00:00

  • Three fatal drug overdoses involving bupropion.

    abstract：:Bupropion is a "second generation" antidepressant agent structurally related to the phenethylamines. Postmortem toxicology data are presented from three suicidal drug overdoses in which bupropion was detected. In two cases in which bupropion was the major toxicology finding, peripheral blood levels of the parent drug ...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/17.7.436

    authors： Friel PN,Logan BK,Fligner CL

    更新日期：1993-11-01 00:00:00

  • False carbamazepine positives due to 10,11-dihydro-10-hydroxycarbamazepine breakdown in the GC-MS injector port.

    abstract：:During the investigation of aviation accidents, postmortem specimens from accident victims are submitted to the Federal Aviation Administration's Civil Aerospace Medical Institute (CAMI) for toxicological analysis. A case recently received by CAMI screened positive for the anticonvulsant medication carbamazepine (CBZ;...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bku064

    authors： Lewis RJ,Angier MK,Johnson RD

    更新日期：2014-10-01 00:00:00

  • Determination of tapentadol and its metabolite N-desmethyltapentadol in urine and oral fluid using liquid chromatography with tandem mass spectral detection.

    abstract：:An analytical procedure for the determination of the new pain medication tapentadol and its main metabolite N-desmethyltapentadol (DMT), in urine and oral fluid has been developed and validated using liquid chromatography with tandem mass spectral detection (LC-MS-MS). Oral fluid was collected using Quantisal™ devices...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/34.8.458

    authors： Coulter C,Taruc M,Tuyay J,Moore C

    更新日期：2010-10-01 00:00:00

  • Determination of opiates and cocaine and its metabolites in biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry.

    abstract：:A rapid, sensitive, and specific method for the determination of opiates and cocaine and metabolites in urine, plasma, and blood was established. A one-step extraction followed by liquid chromatography-electrospray ionization tandem mass spectrometry operating in multiple reaction monitoring mode was used. Two chromat...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/23.7.620

    authors： Cailleux A,Le Bouil A,Auger B,Bonsergent G,Turcant A,Allain P

    更新日期：1999-11-01 00:00:00

  • Detection of Nutmeg Abuse by Gas Chromatography-Mass Spectrometric Screening of Urine.

    abstract：:High doses of nutmeg (seeds from Myristica fragrans Houtt.) can be abused as a psychoactive drug due to phenylpropene ingredients. During controlled abstinence, e.g., in forensic psychiatric clinics, nutmeg abuse has to be distinguished from an ingestion of other spices having phenylpropene ingredients (e.g., black pe...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bkz054

    authors： Neukamm MA,Schwelm HM,Vieser S,Schiesel N,Auwärter V

    更新日期：2020-01-07 00:00:00

  • Analysis of 3,4-methylenedioxymethamphetamine (MDMA) and its metabolites in plasma and urine by HPLC-DAD and GC-MS.

    abstract：:In Europe, the compound 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy, Adam), in addition to cannabis, is the most abused illicit drug at all-night "techno" parties. Methods for the determination of MDMA and its metabolites, 4-hydroxy-3-methoxymethamphetamine (HMMA), 3,4-dihydroxy-methamphetamine (HHMA), 3,4-methyl...

    journal_title：Journal of analytical toxicology

    pub_type： 临床试验,杂志文章

    doi：10.1093/jat/20.6.432

    authors： Helmlin HJ,Bracher K,Bourquin D,Vonlanthen D,Brenneisen R

    更新日期：1996-10-01 00:00:00

  • fMRI: a new tool for the in vivo localization of drug actions in the brain.

    abstract：:Functional magnetic resonance imaging (fMRI), a still-emerging, non-invasive neuroimaging tool, has been applied to a wide range of questions in sensory, motor control, and cognitive psychology. Only more recently has it been applied to understand the sites and mechanisms of action of pharmacological agents within the...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章,评审

    doi：10.1093/jat/25.5.419

    authors： Stein EA

    更新日期：2001-07-01 00:00:00

  • Discrimination between patterns of drug exposure by toxicological analysis of decomposed skeletal tissues. Part II: Amitriptyline and citalopram.

    abstract：:Decomposed bone and plasma samples of rats exposed to amitriptyline (AMI) and citalopram (CIT) under different dosing patterns were analyzed. Wistar rats received one acute dose (120 mg AMI/kg and 40 mg CIT/kg; n = 5) or two doses (40 mg AMI/kg and 13 mg CIT/kg, n = 5) 40 min apart. After collection of perimortem bloo...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bkt078

    authors： Watterson JH,Cornthwaite HM

    更新日期：2013-10-01 00:00:00

  • Determination of benzodiazepines in oral fluid using LC-MS-MS.

    abstract：:A rapid, simple, highly sensitive procedure for the simultaneous analysis of bromazepam, alprazolam, clonazepam, lorazepam, oxazepam, diazepam, midazolam, flurazepam, flunitrazepam, nordiazepam, triazolam, temazepam, nitrazepam, and chlordiazepoxide in oral fluid is described, using liquid chromatography coupled to a ...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/31.9.596

    authors： Moore C,Coulter C,Crompton K,Zumwalt M

    更新日期：2007-11-01 00:00:00

  • Maprotiline fatality: case report and analytical determinations.

    abstract：:A new tetracyclic antidepressant drug, maprotiline hydrochloride was found to be the agent of death in a 23-year-old subject following massive injestion (4.5-6.0 g). The case may be the first reported maprotiline death (sole cause) in the United States. Analytical parameters were studies and various chromatographic an...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/6.4.199

    authors： Rejent TA,Doyle RE

    更新日期：1982-07-01 00:00:00

  • Evaluation of the Abbott ADx Amphetamine/Methamphetamine II abused drug assay: comparison to TDx, EMIT, and GC/MS methods.

    abstract：:Although the legitimate clinical use of amphetamine and amphetamine congeners is declining, the illicit use of these drugs remains high. There is a need for a rapid and conclusive method for detecting these compounds in routine urine drug testing, drug screening in drug rehabilitation centers, and as an aid in the dia...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/15.6.323

    authors： Przekop MA,Manno JE,Kunsman GW,Cockerham KR,Manno BR

    更新日期：1991-11-01 00:00:00

  • Detection of Nitrobenzodiazepines and Their 7-Amino Metabolites in Oral Fluid.

    abstract：:Clonazepam, nitrazepam and flunitrazepam are frequently used benzodiazepines, both as prescribed medication and as drugs of abuse. Little is, however, known about how these drugs are excreted in oral fluid. It has been claimed that the parent drugs are more likely to be detected in oral fluid than the 7-amino metaboli...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bkw020

    authors： Vindenes V,Strand DH,Koksæter P,Gjerde H

    更新日期：2016-05-01 00:00:00

  • Quantitation of dialkyl phosphate metabolites of organophosphate pesticides in human urine using GC-MS-MS with isotopic internal standards.

    abstract：:Human exposure to organophosphate pesticides can be estimated from the presence of urinary metabolites. An isotope-dilution gas chromatography-tandem mass spectrometry (GC-MS-MS) method was developed for quantitating the six dialkyl phosphate urinary metabolites of at least 29 organophosphate pesticides. Urine samples...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/26.5.245

    authors： Bravo R,Driskell WJ,Whitehead RD Jr,Needham LL,Barr DB

    更新日期：2002-07-01 00:00:00

  • Validation of a new homogeneous immunoassay for the detection of carisoprodol in urine.

    abstract：:The objective of this project was to validate a new high-throughput homogeneous enzyme immunoassay (HEIA) for the rapid detection of carisoprodol in human urine. Carisoprodol (Soma(®)) and meprobamate are widely prescribed as musculoskeletal pain relief drugs and are listed as one of the 10 most frequently identified ...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/anatox/35.2.108

    authors： Wang G,Huynh K,Barhate R,Rodrigues W,Moore C,Coulter C,Vincent M,Soares J

    更新日期：2011-03-01 00:00:00

  • Screening of basic drugs in biological samples using dual column capillary chromatography and nitrogen-phosphorus detectors.

    abstract：:A method is presented for the extraction and screening of basic and neutral drugs, including amphetamines. Two milliliters of blood are buffered to pH 9.0 and extracted with n-chlorobutane. Underivatized extracts are analyzed simultaneously on two complementary capillary columns placed together in one injection port u...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/10.5.198

    authors： Watts VW,Simonick TF

    更新日期：1986-09-01 00:00:00

  • Determination of phenol and pentachlorophenol in plasma and urine samples by gas liquid chromatography.

    abstract：:Gas chromatographic methods have been developed using a Supelco 1240-DA column for the determination of pentachlorophenol (PCP) in plasma and urine samples, and phenol in urine samples. These methods required no derivatization. Electron capture detection has been used for PCP determination, while flame ionization dete...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/6.6.297

    authors： Rick DL,Licht CF,McCarty LP

    更新日期：1982-11-01 00:00:00

  • Analysis of Dextromethorphan and Dextrorphan in Skeletal Remains Following Decomposition in Different Microclimate Conditions.

    abstract：:The effects of decomposition microclimate on the distribution of dextromethorphan (DXM) and dextrorphan (DXT) in skeletonized remains of rats acutely exposed to DXM were examined. Animals (n = 10) received DXM (75 mg/kg, i.p.), were euthanized 30 min post-dose and immediately allowed to decompose at either Site A (sha...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bkw083

    authors： Unger KA,Watterson JH

    更新日期：2016-10-01 00:00:00

  • Electrothermal atomic absorption spectrometric determination of cadmium and nickel in urine.

    abstract：:This paper describes two relatively simple methods for the microdetermination of urinary cadmium (U-Cd) and urinary nickel (U-Ni) using electrothermal atomic absorption spectrometry. Reduction of nonspecific absorption interferences was achieved by temperature programming and background correction using a deuterium ar...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/14.1.29

    authors： Ong CN,Chua LH,Lee BL,Ong HY,Chia KS

    更新日期：1990-01-01 00:00:00

  • Stability of Synthetic Piperazines in Human Whole Blood.

    abstract：:While circumventing legislative controls, synthetic piperazines are encountered as "legal" alternatives to ecstasy. Unforeseeable challenges may delay quantitative analysis of these compounds in biological fluids. Enzymatic reactions, matrix interferences and limited knowledge of analyte stability further complicate i...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bkx090

    authors： Lau T,LeBlanc R,Botch-Jones S

    更新日期：2018-03-01 00:00:00

  • Development and Validation of an Analytical Method for Volatiles with Endogenous Production in Putrefaction and Submersion Situations.

    abstract：:A group of 16 volatile substances (ethyl acetate, 2-propanol, 1-propanol, methanol, acetone, ethanol, acetaldehyde, diethyl ether, methyl ethyl ketone, 1-butanol, 2-butanol, t-butanol, isobutanol, 2-methyl-1-butanol, 3-methyl-1-butanol, 1-pentanol) were qualitatively and quantitatively analyzed through a method develo...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/bkaa154

    authors： Pinto M,Eusébio E,Monteiro C

    更新日期：2020-10-08 00:00:00

  • Comparison of EMIT II, CEDIA, and DPC RIA assays for the detection of lysergic acid diethylamide in forensic urine samples.

    abstract：:In an effort to determine a practical, efficient, and economical alternative for the use of a radioimmunoassay (RIA) for the detection of lysergic acid diethylamide (LSD) in human urine, the performance of two photometric immunoassays (Dade Behring EMIT II and Microgenics CEDIA) and the Diagnostics Products Corp. (DPC...

    journal_title：Journal of analytical toxicology

    pub_type： 杂志文章

    doi：10.1093/jat/26.7.519

    authors： Wiegand RF,Klette KL,Stout PR,Gehlhausen JM

    更新日期：2002-10-01 00:00:00