Abstract:
:Human exposure to organophosphate pesticides can be estimated from the presence of urinary metabolites. An isotope-dilution gas chromatography-tandem mass spectrometry (GC-MS-MS) method was developed for quantitating the six dialkyl phosphate urinary metabolites of at least 29 organophosphate pesticides. Urine samples were spiked with stable isotope analogues of the dialkyl phosphates, evaporated using azeotropic distillation, followed by chemical derivatization of the metabolites to their respective chloropropyl phosphate esters. The chloropropyl phosphate esters were concentrated and then analyzed using GC-MS-MS. The limits of detection (LODs) of the method were in the low-to-mid picogram-per-milliliter range (parts per trillion) with coefficients of variation of less than 20%. The use of stable isotope analogues as internal standards for each of these metabolites allows for the highest degree of accuracy and precision. Additionally, the low LODs allow the use of this method in general population studies.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Bravo R,Driskell WJ,Whitehead RD Jr,Needham LL,Barr DBdoi
10.1093/jat/26.5.245keywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
245-52issue
5eissn
0146-4760issn
1945-2403journal_volume
26pub_type
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