Abstract:
:The Abbott TDx Urine Amphetamine/Methamphetamine II fluorescence polarization immunoassay technique was applied to the determination of D-methamphetamine in hemolyzed whole blood. The assay was found to have 100% cross-reactivity with D-methamphetamine and only an 8% cross-reactivity with L-methamphetamine. Whole blood was fortified with D-methamphetamine at concentrations ranging from 25 to 1000 ng/mL. These whole blood calibrators were used to evaluate the following sample preparation techniques: direct, diluted and buffer, and precipitated using methanol, acetone, sulfosalicylic acid, trichloroacetic acid, and zinc sulfate. Calibrators and samples were prepared by mixing 200 microL of whole blood and 200 microL precipitation reagent and centrifuging at 10,000 rpm for 5 min (9600 x g). A 50-microL aliquot of the supernatant was used for the assay. Using the zinc sulfate precipitation, blood calibration curves showed a linear range of 25-1000 ng/mL. The within-run precision for the 25-, 60-, and 200-ng/mL D-methamphetamine blood controls showed percent coefficients of variation of 17.8, 17.0, and 5.4, respectively. The TDx results were compared to RIA and GC/MS assays for the methamphetamine controls and for eight positive case specimens and found reliable for the screening of hemolyzed whole blood.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Simonick TF,Watts VWdoi
10.1093/jat/16.2.115keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
115-8issue
2eissn
0146-4760issn
1945-2403journal_volume
16pub_type
杂志文章abstract::A group of 16 volatile substances (ethyl acetate, 2-propanol, 1-propanol, methanol, acetone, ethanol, acetaldehyde, diethyl ether, methyl ethyl ketone, 1-butanol, 2-butanol, t-butanol, isobutanol, 2-methyl-1-butanol, 3-methyl-1-butanol, 1-pentanol) were qualitatively and quantitatively analyzed through a method develo...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkaa154
更新日期:2020-10-08 00:00:00
abstract::An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method was developed and validated for the simultaneous determination of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-Delta(9)-tetrahydrocannabinol (11-OH-THC), and 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH) in whole blo...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/32.5.349
更新日期:2008-06-01 00:00:00
abstract::A 24-year-old man known to consume illegal drugs was found dead in his apartment. A reclosable plastic zipper bag containing several hundred milligrams of a brown powder was found close to the dead body and the first assumption of the investigators was death due to heroin intoxication. Therefore, a legal autopsy was o...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkw096
更新日期:2016-11-01 00:00:00
abstract::The synthetic chemical 4-aminobiphenyl (4-ABP) has been shown to be a bladder carcinogen in both man and animals. A valid radioimmunoassay for a metabolite of 4-ABP has been developed as a means to monitor potential human exposure to 4-ABP. The antibody was produced by the immunization of two female New Zealand White ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/4.2.86
更新日期:1980-03-01 00:00:00
abstract::A case report involving a 34-year-old white male who was found dead at home by his roommate is presented. At the time of his death, he was being treated with tramadol/acetaminophen, metaxalone, oxycodone, and amitriptyline. The decedent's mother stated that he had been taking increasing amounts of pain medication in o...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/29.5.401
更新日期:2005-07-01 00:00:00
abstract::We report one fatal case of asphyxia caused by leaves of common ivy. Macroscopic examination of the corpse during the autopsy disclosed an incredible quantity of leaves of Hedera helix in the mouth and throat of the decedent. In order to rule out the possibility of poisoning by the toxic saponins contained in the plan...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/27.4.257
更新日期:2003-05-01 00:00:00
abstract::Synthetic cathinones have recently emerged as a substitute for common drugs of abuse. Synthetic cathinones can elicit powerful adverse effects such as delusions, hallucinations and potentially dangerous behavior. To develop a method to analyze 10 synthetic cathinones in oral fluid, we implemented a combined approach o...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkt080
更新日期:2013-11-01 00:00:00
abstract::This paper presents a case of fatal overdosage due to an accidental massive administration (750 mg instead of 170 mg) of cisplatin, an anticancer agent, to a 63-year-old patient suffering from lymphoma. Platinum was measured in various postmortem samples by means of inductively coupled plasma mass spectrometry. Heart ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/28.2.138
更新日期:2004-03-01 00:00:00
abstract::A rapid and simple liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of L-ephedrine, pseudoephedrine, and caffeine in male Fisher-344 rat plasma at nanogram-per-milliliter concentrations for use in support of toxicology studies. Only 25 μL of plasma is...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/anatox/35.6.341
更新日期:2011-07-01 00:00:00
abstract::In previous experimental studies on heroin metabolites excretion in urine, the first sample was often collected a few hours after intake. In forensic cases, it is sometimes questioned if a positive urine result is expected e.g., 30 min after intake. The aim of this study was to investigate urinary excretion of heroin ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkx017
更新日期:2017-06-01 00:00:00
abstract::Conditions for the enzymatic hydrolysis of benzodiazepine conjugates were systematically examined. Optimal recovery of the free drugs occurs when 1 mL of urine buffered to pH 4.5 is incubated with 5000 U of Helix pomatia beta-glucuronidase at 56 degrees C for 2 h. Urine specimens containing conjugates of temazepam, ox...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/18.7.382
更新日期:1994-11-01 00:00:00
abstract::A new tetracyclic antidepressant drug, maprotiline hydrochloride was found to be the agent of death in a 23-year-old subject following massive injestion (4.5-6.0 g). The case may be the first reported maprotiline death (sole cause) in the United States. Analytical parameters were studies and various chromatographic an...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/6.4.199
更新日期:1982-07-01 00:00:00
abstract::A rapid, simple, highly sensitive procedure for the simultaneous analysis of bromazepam, alprazolam, clonazepam, lorazepam, oxazepam, diazepam, midazolam, flurazepam, flunitrazepam, nordiazepam, triazolam, temazepam, nitrazepam, and chlordiazepoxide in oral fluid is described, using liquid chromatography coupled to a ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/31.9.596
更新日期:2007-11-01 00:00:00
abstract::Phenazepam and etizolam were the first uncontrolled benzodiazepines available for sale in the UK. Pyrazolam, flubromazepam and diclazepam are not used medicinally anywhere in the world; they are produced exclusively for the uncontrolled, recreational market. It is important to know whether potentially abused drugs lik...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkv122
更新日期:2016-03-01 00:00:00
abstract::New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkaa089
更新日期:2020-10-12 00:00:00
abstract::A sensitive method for identifying and quantifying brucine by means of liquid chromatography-tandem mass spectrometry is presented in this article. Based on a solid-phase extraction for human serum, the validation indicated limits of detection and quantification of 0.12 and 0.23 ng/mL, respectively. In one case of let...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/anatox/35.4.248
更新日期:2011-05-01 00:00:00
abstract::Methyl methacrylate polymer (PolyMMA) is widely used as the composite resin for the dental plate. During the fabrication process of PolyMMA for the polymerization reaction, benzoylperoxide (BPO) and N,N-dimethyl p-toluidine (DMPT) are added as the initiator and the stimulator, respectively. Because these compounds exh...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/17.2.73
更新日期:1993-03-01 00:00:00
abstract::Urine drug testing (UDT) is an emerging standard of care in the evaluation and treatment of chronic non-cancer pain patients with opioid analgesics. UDT may be used both to verify adherence with the opioid analgesic regimen and to monitor abstinence from non-prescribed or illicit controlled substances. In the former s...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bku109
更新日期:2015-01-01 00:00:00
abstract::A simple, rapid ethanol screen based on a microdiffusion technique (Analytical Systems, Marion Laboratories) has been evaluated and compared to an established enzymatic procedure (Abbott TDx). They showed agreement in 484 of 488 biological specimens and all control specimens. They disagreed on 4 specimens--3 positive ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/12.6.348
更新日期:1988-11-01 00:00:00
abstract::Previous studies have shown that drug concentrations in blood can change during storage, especially at room temperature, but even labile drugs such as cocaine may be stable in dried blood spots (DBS). A new method has been developed for the analysis of hydrolytically labile drugs in blood spots on filter paper in orde...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/32.7.511
更新日期:2008-09-01 00:00:00
abstract::Analytical methods were developed to determine the presence of ethylenediaminetetraacetic acid (EDTA) in dried bloodstains to provide probative information when allegations of evidence tampering have been made in criminal cases. A simple screening method using ion chromatography to analyze stains was found to be quant...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/21.7.521
更新日期:1997-11-01 00:00:00
abstract::During periodate degradation of interfering ephedrine, pseudoephedrine, and phenylpropanolamine in the extraction of methamphetamine from urine, it was observed that a small amount of methamphetamine was demethylated to amphetamine. although all three interfering phenylpropanolamines could be degraded by periodate at ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/18.6.331
更新日期:1994-10-01 00:00:00
abstract::A gas chromatographic-chemical ionization mass spectrometric (GC-CIMS) method is described for the determination of cocaine, benzoylecgonine, and norcocaine. The procedure uses stable isotopes as internal standards and a mixture of methane-ammonia as chemical ionization reagent gas. Run-to-run and within-run coefficie...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/4.1.37
更新日期:1980-01-01 00:00:00
abstract::At the end of 2010, the U.S. Drug Enforcement Administration (DEA) used its emergency scheduling authority to temporarily control five chemicals, JWH-018, JWH-073, JWH-200, CP-47497, and cannabicyclohexanol (CP-47497 C8), often referred to as "Spice", K2, or "synthetic cannabinoids" because of their reported cannabis-...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/anatox/35.7.424
更新日期:2011-09-01 00:00:00
abstract::Exemplars of current commercial breath-alcohol simulators were studied to ascertain their suitability for control tests and as calibrators in breath-alcohol analysis. Temperature, effluent volumes and pressures, effluent-alcohol concentration, and alcohol depletion were measured for simulators coupled with various cur...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/15.5.272
更新日期:1991-09-01 00:00:00
abstract::Quantitative analysis of Δ9-tetrahydrocannabinol (Δ9-THC) in oral fluid has gained increasing interest in clinical and forensic toxicology laboratories. New medicinal and/or recreational cannabinoid products require laboratories to distinguish different patterns of cannabinoid use. This study validated a high-performa...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkaa184
更新日期:2020-12-03 00:00:00
abstract::A selective, sensitive, and reliable method was devised to determine concentrations of amitriptyline and its major metabolite, nortriptyline, in human plasma using high-performance liquid chromatography (HPLC) combined with UV and particle beam mass spectrometry (PBMS). Amitriptyline and nortriptyline were effectively...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/21.3.185
更新日期:1997-05-01 00:00:00
abstract::While earlier studies have attempted to resolve the challenges encountered when interpreting gamma-hydroxybutyric acid (GHB) concentrations in hair (primarily due to its endogenous presence), few have had large sample sizes. The first objective of this study was to evaluate the inter-individual variation of endogenous...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkaa080
更新日期:2020-10-12 00:00:00
abstract::To determine the degree of ethanol absorption and the resultant formation and urinary excretion of its conjugated metabolites following intensive use of high ethanol content mouthwash, 10 subjects gargled with Listerine(®) antiseptic 4 times daily for 3¼ days. First morning void urine specimens were collected on each ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/anatox/35.5.264
更新日期:2011-06-01 00:00:00
abstract::Amoxicillin has been causally linked in the lay and medical literature to false-positive urine drug screens for cocaine metabolites. An exhaustive search of the peer-reviewed medical literature revealed no data to support this link. We hypothesized that amoxicillin does not cause false-positive urine drug screens for ...
journal_title:Journal of analytical toxicology
pub_type: 临床试验,杂志文章
doi:10.1093/jat/32.4.315
更新日期:2008-05-01 00:00:00