Abstract:
:Conditions for the enzymatic hydrolysis of benzodiazepine conjugates were systematically examined. Optimal recovery of the free drugs occurs when 1 mL of urine buffered to pH 4.5 is incubated with 5000 U of Helix pomatia beta-glucuronidase at 56 degrees C for 2 h. Urine specimens containing conjugates of temazepam, oxazepam, lorazepam, alpha-hydroxyalprazolam, 2-hydroxyethylflurazepam, and N-desalkyl-3-hydroxyflurazepam as targeted benzodiazepines were used. The freed drugs were quantitated by gas chromatography-mass spectrometry.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Meatherall Rdoi
10.1093/jat/18.7.382subject
Has Abstractpub_date
1994-11-01 00:00:00pages
382-4issue
7eissn
0146-4760issn
1945-2403journal_volume
18pub_type
杂志文章abstract::We present an analytical method for the determination of heroin, free 6-acetylmorphine (6-AM), and free morphine in blood, urine, and vitreous humor. This method is used in postmortem cases where heroin is the suspected cause of death. The analytical protocol includes the following steps: liquid-liquid extraction at p...
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