Abstract:
:The concentrations of amphetamine and methamphetamine in a new lyophilized human urine reference material (RM) were determined at the National Institute of Standards and Technology, in cooperation with the College of American Pathologists. In order to minimize the possibility of undetected bias, two different methods were developed. Both methods were based on isotope-dilution gas chromatography/mass spectrometry (GC/MS) with a deuterium-labeled standard. The first method used a solid phase extractor (SPE) to isolate the analytes, followed by forming the heptafluorobutyryl (HFB) derivatives for measurement by GC/MS. The second method used another kind of SPE to isolate the analytes, followed by forming the N-trifluoroacetyl-1-prolyl chloride (TPC) derivatives for measurement by GC/MS. The RM consists of three levels of amphetamine, three levels of methamphetamine, and a blank. For amphetamine, the concentrations are 291, 558, and 1081 ng/mL of human urine. For methamphetamine, the concentrations are 311, 573, and 1137 ng/mL of human urine.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Ellerbe P,Long T,Welch MJdoi
10.1093/jat/17.3.165subject
Has Abstractpub_date
1993-05-01 00:00:00pages
165-70issue
3eissn
0146-4760issn
1945-2403journal_volume
17pub_type
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