Abstract:
:The Connecticut Department of Public Safety laboratory recently addressed a legal challenge to a hospital alcohol dehydrogenase (ADH)-based serum ethanol determination based on the suggestion of interference by lactate dehydrogenase (LDH)-catalyzed oxidation of lactate. Both ADH- and LDH-oxidations require NAD(+) (present in excess in the assay). NADH produced by LDH-catalyzed lactate oxidation in the assay is interpreted as derived from ethanol. Hepatic trauma was suggested as the basis for elevated levels of lactate and LDH. Clinical laboratory results were evaluated, specifically serum hepatic enzymes, ions, and anion gap. Aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) were 229 and 144 U/L, respectively (approximately 8x and 4x reference range midpoint values). Na(+), K(+), Cl(-), and CO(2) levels were 143, 3.0, 112, and 20 meq/L, respectively, yielding an anion gap of 8 meq/L (ref. range 8-15). Serum lactate contributes to "unmeasured anions"; hence, the anion gap was inconsistent with a significant lactate elevation. Based on the slight elevation of ASAT and ALAT, LDH levels were estimated to be elevated to no more than 10-fold. Calculation of the amount of LDH and ADH present in the ethanol assay suggest an ADH/LDH ratio of 200:1. Hence, contribution by lactate oxidation to the serum ethanol concentration in this case would have been negligible.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Powers RH,Dean DEdoi
10.1093/jat/33.8.561subject
Has Abstractpub_date
2009-10-01 00:00:00pages
561-3issue
8eissn
0146-4760issn
1945-2403journal_volume
33pub_type
杂志文章abstract::In this study, the relationships between the concentrations of R/S-methamphetamine (MA) and its metabolite R/S-amphetamine (AP), the AP/MA ratio in hair samples, and MA dependence were investigated by performing segmental hair analysis in MA users. Authentic hair samples collected from 10 chronic MA abusers were cut i...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkz089
更新日期:2020-07-31 00:00:00
abstract::An existing GC-MS method for detecting benzoylecgonine (BZE) in urine was modified by changing derivatizing reagents. This method modification presents a cost-effective alternative derivatization procedure for the detection of BZE in urine by GC-MS. The combination of pentafluoropropanol and acetic anhydride was found...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkx007
更新日期:2017-05-01 00:00:00
abstract::A liquid chromatography-tandem mass spectrometry method was developed for the simultaneous quantification of 26 benzodiazepines and metabolites, zolpidem and zopiclone, in blood, urine, and hair. Drugs were extracted from all matrices by liquid-liquid extraction with 1-chlorobutane. Chromatography was achieved using a...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/29.7.616
更新日期:2005-10-01 00:00:00
abstract::A 59-year-old white male accidentally ingested a mouthful of a plant growth chemical, Cycocel, containing 11.8% of the active ingredient (2-chloroethyl)trimethylammonium chloride (chlormequat). He was seen by a family physician and then transferred to a hospital where he died as a result of ventricular fibrillation, w...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/14.4.257
更新日期:1990-07-01 00:00:00
abstract::We developed a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method to measure metabolites of atrazine, phenylurea, and sulfonylurea herbicides in human urine. The metabolites measured in the method include atrazine mercapturate, desethyl atrazine, and desisopropyl atrazine as markers of...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/31.4.181
更新日期:2007-05-01 00:00:00
abstract::In Europe, the compound 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy, Adam), in addition to cannabis, is the most abused illicit drug at all-night "techno" parties. Methods for the determination of MDMA and its metabolites, 4-hydroxy-3-methoxymethamphetamine (HMMA), 3,4-dihydroxy-methamphetamine (HHMA), 3,4-methyl...
journal_title:Journal of analytical toxicology
pub_type: 临床试验,杂志文章
doi:10.1093/jat/20.6.432
更新日期:1996-10-01 00:00:00
abstract::In order to achieve accurate quantitation of drugs and metabolites (analytes) in complex matrices, 2H- (and less commonly 13C-) labeled analogues of the analytes are now routinely adapted as the internal standards (IS) using linear calibration models to fit data generated by selected ion monitoring gas chromatography-...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/25.3.179
更新日期:2001-04-01 00:00:00
abstract::During periodate degradation of interfering ephedrine, pseudoephedrine, and phenylpropanolamine in the extraction of methamphetamine from urine, it was observed that a small amount of methamphetamine was demethylated to amphetamine. although all three interfering phenylpropanolamines could be degraded by periodate at ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/18.6.331
更新日期:1994-10-01 00:00:00
abstract::A fatality due to the ingestion of isoniazid, a tuberculostatic agent, is presented. Isoniazid was extracted by a single step extraction procedure, derivatized with trifluoroacetic anhydride, and identified and quantified by gas chromatography/mass spectrometry (GC/MS). The distribution of isoniazid was as follows: he...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/16.1.57
更新日期:1992-01-01 00:00:00
abstract::A method for the determination of the antidepressant drug trazodone is presented. 8-Hydroxyloxapine is used as the internal standard. A simple solid-phase extraction procedure utilizing disposable reversed-phase C18 columns is described. Samples are analyzed by gas chromatography with nitrogen-selective detection usin...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/12.3.150
更新日期:1988-05-01 00:00:00
abstract::Methoxphenidine (MXP, 2-MeO-diphenidine) is a dissociative anaesthetic drug of the diarylethylamine type, recently introduced for recreational purposes through the online-based sale of new psychoactive substances (NPS). The concentration of MXP in hair has never been reported, either in cases of chemsex use or in fata...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkab016
更新日期:2021-02-01 00:00:00
abstract::Calibration model selection is required for all quantitative methods in toxicology and more broadly in bioanalysis. This typically involves selecting the equation order (quadratic or linear) and weighting factor correctly modelizing the data. A mis-selection of the calibration model will generate lower quality control...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkx001
更新日期:2017-05-01 00:00:00
abstract::The application of gas chromatography/mass spectrometry (GC/MS) for the simultaneous quantitation of seven commonly encountered urinary benzodiazepine metabolites is described. After comparison of the signal-to-noise ratios of high mass ions of benzodiazepines using electron impact (EI), positive chemical ionization (...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/17.6.342
更新日期:1993-10-01 00:00:00
abstract::A simple, rapid, and sensitive method which allows us to simultaneously determine bromvalerylurea (BVU) and its three metabolites (3-methylbutyrylurea [MVU], alpha-(cystein-S-yl)isovalerylurea [CVU], and alpha-(N-acetylcystein-S-yl)isovalerylurea [AcCVU]) was investigated by frit-fast atom bombardment liquid chromatog...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/22.7.591
更新日期:1998-11-01 00:00:00
abstract::From a public health point of view, cocaine (COC) presents serious clinical problems and deaths from overdose and lifelong addiction patterns, not to mention its involvement in crime, in the United States. This study subjected rhesus monkeys to one intravenous administration of COC (1 mg/kg), which closely imitates th...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/19.7.571
更新日期:1995-11-01 00:00:00
abstract::The percent recovery of mercury from human hair digest samples, using the peak height cold-vapor atomic adsorption method is 73.0% +/- 10.3%. This value and its reproducibility are raised to 102.2% +/- 6.3% by use of peak area measurements in place of peak height. The so-called matrix effect is thus eliminated, and it...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/5.1.52
更新日期:1981-01-01 00:00:00
abstract::We report one fatal case of asphyxia caused by leaves of common ivy. Macroscopic examination of the corpse during the autopsy disclosed an incredible quantity of leaves of Hedera helix in the mouth and throat of the decedent. In order to rule out the possibility of poisoning by the toxic saponins contained in the plan...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/27.4.257
更新日期:2003-05-01 00:00:00
abstract::Estimating the equivalent whole blood ethanol level from a serum or plasma determination has been addressed by numerous articles in both the clinical and forensic literature. All previous studies have either involved sample sizes insufficient for adequate statistical evaluation or have utilized gas chromatography for ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/31.1.23
更新日期:2007-01-01 00:00:00
abstract::The collection and analysis of drugs in oral fluid (OF) at the roadside has become more feasible with the introduction of portable testing devices such as the Alere™ DDS®2 Mobile Test System (DDS®2). The objective of this study was to compare the on-site results for the DDS®2 to laboratory-based confirmatory assays wi...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkx105
更新日期:2018-04-01 00:00:00
abstract::A blood concentration of tetrahydrocannabinol (THC) in the low nanograms-per-milliliter range is often claimed to result from drug use more than 24-48 h previously. The present investigation determined concentrations of cannabinoids in blood collected at least 24 h from smoking in an in-patient setting. During samplin...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/32.2.160
更新日期:2008-03-01 00:00:00
abstract::The fraction of any surface-adsorbed contaminant available for absorption is considered the bioaccessible fraction. Applied previously to contaminants such as pesticides and heavy metals on surfaces such as soil, food and cosmetics, the term may also be used to describe the fraction of drug residue bound to work surfa...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bky073
更新日期:2019-03-01 00:00:00
abstract::Benzoylecgonine (BZE) extraction from urine was explored using Cerex Polycrom Clin II solid-phase extraction (SPE) columns and the Speedisk 48 Pressure Processor as an alternative to the Prep1 automated sample processor and XTRX Type RP/W columns. Linearity for urine standards extracted using the Cerex-Speedisk method...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/25.7.616
更新日期:2001-10-01 00:00:00
abstract::This report describes a possible suicide by overdosage with diltiazem. The decreased, a 39-year-old male who had been under treatment for an unidentified heart condition, was discovered on the bathroom floor in his residence. Comprehensive drug screens performed on available postmortem specimens revealed the presence ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/18.3.180
更新日期:1994-05-01 00:00:00
abstract::An analytical procedure for the determination of the new pain medication tapentadol and its main metabolite N-desmethyltapentadol (DMT), in urine and oral fluid has been developed and validated using liquid chromatography with tandem mass spectral detection (LC-MS-MS). Oral fluid was collected using Quantisal™ devices...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/34.8.458
更新日期:2010-10-01 00:00:00
abstract::Prolintane is a sympathomimetic amine with pharmacologic properties similar to d-amphetamine. Side effects include insomnia, nervousness, and irritability. Overdoses of prolintane may cause hallucinations, psychosis, and death. The drug is commonly prescribed in Africa, Australia, and Europe but is not available in th...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/31.7.415
更新日期:2007-09-01 00:00:00
abstract::Because mycotoxins occur worldwide in grain and grain products, evaluating their effects on the health of the population has become important. The development of a high performance liquid chromatographic (HPLC) procedure was investigated for the analysis of aflatoxin B1, citrinin, and ochratoxin A, from hydrolyzed hum...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/10.2.41
更新日期:1986-03-01 00:00:00
abstract::An automated solid-phase extraction (SPE) protocol followed by gas chromatography coupled with tandem mass spectrometry was developed for quantification of caffeine, cyamemazine, meprobamate, morphine and 6-monoacetylmorphine (6-MAM) in 11 biological matrices [blood, urine, bile, vitreous humor, liver, kidney, lung an...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bku029
更新日期:2014-06-01 00:00:00
abstract::Toxicological analyses of medicines and chemicals in formaldehyde-treated specimens are a component of forensic toxicology. This review involves two parts: (i) reactions of formaldehyde with substances in tissues fixed with formalin (aqueous formaldehyde solution) and (ii) the stability of substances in formalin-treat...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章,评审
doi:10.1093/jat/bkt055
更新日期:2013-11-01 00:00:00
abstract::A high-performance liquid chromatographic system based on gradient elution of an alumina packing material coated with polybutadiene is presented. The chromatographic eluent is a mixture of methanol and water, both containing 0.0125M NaOH. The effluent is monitored by photodiode array detection. The system allows detec...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/19.2.73
更新日期:1995-03-01 00:00:00
abstract::Quantitative analysis of Δ9-tetrahydrocannabinol (Δ9-THC) in oral fluid has gained increasing interest in clinical and forensic toxicology laboratories. New medicinal and/or recreational cannabinoid products require laboratories to distinguish different patterns of cannabinoid use. This study validated a high-performa...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkaa184
更新日期:2020-12-03 00:00:00