Abstract：
:A sensitive and robust ultra-high-performance liquid chromatography-tandem mass spectrometry method has been developed and validated for the quantification of acetaminophen, dexchlorpheniramine, caffeine, cotinine and salicylic acid in postmortem blood samples from children younger than 4 years. The sample was prepared by a protein precipitation with ice-cold methanol/acetonitrile mixture (85:15, v/v). The organic phase was evaporated to dryness and the residue was dissolved in the mobile phase. Separation, with gradient elution and an acidic mobile phase, was achieved on an Acquity UPLC® HSS T3 column. The compounds were quantified using a multiple reaction-monitoring mode. Two transitions were monitored for each compound and one for the deuterated internal standards. The mass spectrometric detection in the positive ion mode was performed for all the compounds except salicylic acid which was detected in the negative ionization mode. The limits of quantification were as follows: acetaminophen 0.30 mg/L, dexchlorpheniramine 0.0050 mg/L, caffeine 0.099 mg/L, cotinine 0.00035 mg/L and salicylic acid 1.3 mg/L. Between-assay and within-assay precisions were ≤15% (biases: -10% to 26%) and ≤10%, respectively. Extraction recoveries varied from 93% to 137%. The matrix effects in blood, corrected with deuterated internal standards, were 100% ± 10% for all compounds except dexchlorpheniramine (111%) and caffeine (138%).
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Krpo M,Arnestad M,Karinen Rdoi
10.1093/jat/bkx089subject
Has Abstractpub_date
2018-03-01 00:00:00pages
126-132issue
2eissn
0146-4760issn
1945-2403pii
4656136journal_volume
42pub_type
杂志文章abstract：:The aim of this study is to investigate the distribution of methamphetamine (MA) and its metabolite amphetamine (AP) in rat models of acute and subacute MA-ethanol combination abuse. Rats were fed with 20% ethanol for 4 weeks (chronic active-drinking group), and MA was injected intraperitoneally into chronically drink...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/bkr007
更新日期：2012-01-01 00:00:00
abstract：:The Rapid Stat assay, a point-of-collection drug-testing device for detection of amphetamines, cannabinoids, cocaine, opiates, methadone, and benzodiazepines in oral fluid, was evaluated for cannabis and amphetamine-type stimulants. The Rapid Stat tests (n = 134) were applied by police officers in routine traffic chec...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/34.3.155
更新日期：2010-04-01 00:00:00
abstract：:The synthetic chemical 4-aminobiphenyl (4-ABP) has been shown to be a bladder carcinogen in both man and animals. A valid radioimmunoassay for a metabolite of 4-ABP has been developed as a means to monitor potential human exposure to 4-ABP. The antibody was produced by the immunization of two female New Zealand White ...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/4.2.86
更新日期：1980-03-01 00:00:00
abstract：:Amoxicillin has been causally linked in the lay and medical literature to false-positive urine drug screens for cocaine metabolites. An exhaustive search of the peer-reviewed medical literature revealed no data to support this link. We hypothesized that amoxicillin does not cause false-positive urine drug screens for ...
journal_title：Journal of analytical toxicology
pub_type： 临床试验,杂志文章
doi：10.1093/jat/32.4.315
更新日期：2008-05-01 00:00:00
abstract：:A forensic standard procedure is described that combines enzyme-linked immunoassay for screening and gas chromatography-mass spectrometry (GC-MS) for confirmation to detect drugs of abuse in a sample before used to detect opioids and cocaine. We used two equal aliquots of the same previously selected cannabinoid posit...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/bkv120
更新日期：2016-07-01 00:00:00
abstract：:In Europe, the compound 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy, Adam), in addition to cannabis, is the most abused illicit drug at all-night "techno" parties. Methods for the determination of MDMA and its metabolites, 4-hydroxy-3-methoxymethamphetamine (HMMA), 3,4-dihydroxy-methamphetamine (HHMA), 3,4-methyl...
journal_title：Journal of analytical toxicology
pub_type： 临床试验,杂志文章
doi：10.1093/jat/20.6.432
更新日期：1996-10-01 00:00:00
abstract：:Bisphenol S (BPS) has been detected in personal care products, water, food and indoor house dust, demonstrating the potential for human exposure. Due to limited data to characterize the hazard of BPS, the National Toxicology Program (NTP) is investigating the toxicity of BPS in rodent models. Generating systemic expos...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/bkab008
更新日期：2021-01-09 00:00:00
abstract：:The abuse of the designer amphetamines such as 3,4-methylenedioxymethamphetamine (MDMA) is increasing throughout the world. They have become popular drugs at all night techno dance parties, and their detection is an important issue. The objective of the presented study was to identify an unknown compound detected by t...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/29.5.353
更新日期：2005-07-01 00:00:00
abstract：:The collection and analysis of drugs in oral fluid (OF) at the roadside has become more feasible with the introduction of portable testing devices such as the Alere™ DDS®2 Mobile Test System (DDS®2). The objective of this study was to compare the on-site results for the DDS®2 to laboratory-based confirmatory assays wi...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/bkx105
更新日期：2018-04-01 00:00:00
abstract：:A high performance liquid chromatographic method is reported, which incorporates three internal standards (I-cinchonidine, N-propylprocainamide, and para-chlorodisopyramide) for the simultaneous quantitation of four commonly prescribed antiarrhythmic drugs: quinidine, procainamide, N-acetylprocainamide, and disopyrami...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/7.2.65
更新日期：1983-03-01 00:00:00
abstract：:A rapid and simple liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of L-ephedrine, pseudoephedrine, and caffeine in male Fisher-344 rat plasma at nanogram-per-milliliter concentrations for use in support of toxicology studies. Only 25 μL of plasma is...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/anatox/35.6.341
更新日期：2011-07-01 00:00:00
abstract：:In methanol intoxication, increased levels of the metabolite formate are associated with metabolic acidosis and an increased risk for ocular and neurological dysfunction. A simple method for plasma formate measurement by adaptation of a manual enzymatic assay to a Cobas Mira S analyzer is presented. Six microliters of...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/25.2.77
更新日期：2001-03-01 00:00:00
abstract：:The aim of this study was to develop, optimize, and validate an analytical procedure to determine a group of eight amphetamines, amphetamine (AMP), ephedrine (EP), methcathinone (MTC), paramethoxyamphetamine (PMA), methamphetamine (MA), methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), and methyle...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/28.2.77
更新日期：2004-03-01 00:00:00
abstract：:Six cases involving the antidepressant mirtazapine were analyzed in detail at the San Diego County Medical Examiner's Office from 2004 to 2005. Mirtazapine was initially detected and confirmed in each of these cases by a liquid-liquid gas chromatography (GC)-MS basic drug screen. Following another liquid-liquid basic ...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/30.9.687
更新日期：2006-11-01 00:00:00
abstract：:Doxepin is a tricyclic antidepressant that is widely prescribed for the treatment of mild depression. In this study, hair samples collected from a patient receiving 25 mg of doxepin daily were analyzed. Doxepin was administered to the patient for 4 months (June 15 to October 15, 1996). Five hair samples were collected...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/22.6.531
更新日期：1998-10-01 00:00:00
abstract：:Quetiapine fumarate (Seroquel) is a dibenzothiazepine psychotropic agent that was introduced in 1997 for treating psychoses. Quetiapine is being found with increasing frequency in postmortem cases in Virginia. We report the postmortem results and histories of 20 quetiapine cases from the Office of the Chief Medical Ex...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/30.4.287
更新日期：2006-05-01 00:00:00
abstract：:Conditions for the enzymatic hydrolysis of benzodiazepine conjugates were systematically examined. Optimal recovery of the free drugs occurs when 1 mL of urine buffered to pH 4.5 is incubated with 5000 U of Helix pomatia beta-glucuronidase at 56 degrees C for 2 h. Urine specimens containing conjugates of temazepam, ox...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/18.7.382
更新日期：1994-11-01 00:00:00
abstract：:A separation system based on isotachophoresis is described for the cyanogenic glycoside linamarin in aqueous solution and in human urine. Isotachophoresis is a migration of a substance in an electric field, which is applied to a system of electrolytes of specific design. Detection is carried out by monitoring conducti...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/18.2.91
更新日期：1994-03-01 00:00:00
abstract：:A 5-mg dose of cocaine free base applied to the volar forearm skin surface of a volunteer resulted in a maximal urinary benzoylecgonine concentration of 55 ng/mL at 48 h, using discrete urine specimens. A total of 58 micrograms of benzoylecgonine, representing 1.2% of the dose, was excreted in the 96-h urine. An ident...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/14.6.383
更新日期：1990-11-01 00:00:00
abstract：:From a public health point of view, cocaine (COC) presents serious clinical problems and deaths from overdose and lifelong addiction patterns, not to mention its involvement in crime, in the United States. This study subjected rhesus monkeys to one intravenous administration of COC (1 mg/kg), which closely imitates th...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/19.7.571
更新日期：1995-11-01 00:00:00
abstract：:A novel mass spectrometry detection technique based on a multi-period and multi- experiment (MRM-EPI-MRM3) with library matching in a single run for fast and rapid screening and identification of amphetamine type stimulants (ATS) related drugs in whole blood, urine and dried blood stain was developed and validated. Th...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/bkz017
更新日期：2019-08-23 00:00:00
abstract：:Metabolism experiments were conducted with rats dosed with nine azo dyes based on dimethyl-, dimethoxy-, or dichlorobenzidine to determine whether the free amine congeners, their monoacetyl or diacetyl metabolites, or alkaline hydrolyzable conjugates were excreted in the urine. After preliminary tests of the dyes, 2-m...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/7.1.55
更新日期：1983-01-01 00:00:00
abstract：:First synthesized in 1970, propafenone is a frequently used 1C antiarrhythmic drug metabolized into two major metabolites, 5-hydroxypropafenone and norpropafenone. Paradoxically, fatal intoxication is rarely described, and only six cases have been reported in the literature. We report our experience with two patients ...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/27.8.595
更新日期：2003-11-01 00:00:00
abstract：:3,4-Methylenedioxymethamphetamine (MDMA), or ecstasy, is excreted as unchanged drug, 3,4-methylenedioxyamphetamine (MDA), and free and glucuronidated/sulfated 4-hydroxy-3-methoxymethamphetamine (HMMA), and 4-hydroxy-3-methoxyamphetamine (HMA) metabolites. The aim of this paper is to describe the pattern and timeframe ...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/33.8.439
更新日期：2009-10-01 00:00:00
abstract：:A method that measures 12 analytes in urine and reflects possible exposure to pesticides was developed. The sample preparation involves enzyme hydrolysis and solvent extraction through the use of laboratory robotics, followed by phase-transfer catalysis derivatization and silica cleanup. Samples are analyzed by capill...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/19.5.323
更新日期：1995-09-01 00:00:00
abstract：:Synthetic cathinones have recently emerged as a substitute for common drugs of abuse. Synthetic cathinones can elicit powerful adverse effects such as delusions, hallucinations and potentially dangerous behavior. To develop a method to analyze 10 synthetic cathinones in oral fluid, we implemented a combined approach o...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/bkt080
更新日期：2013-11-01 00:00:00
abstract：:Two cases of overdose due to ingestion of trazodone are described. In one case, blood collected several hours following ingestion of an estimated 4 or more grams contained a trazodone concentration of 25.7 mg/L. Recovery was uneventful. The second case did not survive and death was attributed to the ingestion of multi...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/8.2.91
更新日期：1984-03-01 00:00:00
abstract：:Publications reporting concentrations of caffeine in postmortem blood were reviewed if the cause of death was attributed to overdosing (poisoning) with drugs. Age and gender of the deceased, the manner of death (accident, suicide or undetermined) and types of co-ingested drugs were evaluated in relation to the concent...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章,评审
doi：10.1093/jat/bkx011
更新日期：2017-04-01 00:00:00
abstract：:There have been numerous reports of lead poisoning resulting from the ingestion of foreign bodies. A case involving the ingestion of spent air rifle pellets is described. No clinical symptoms were observed, despite the fact that the young child exhibited elevated blood lead levels as high as 2.7 micromol/L (56 microg/...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/26.6.370
更新日期：2002-09-01 00:00:00
abstract：:This study identified ethyl sulfate (EtS) in human urine and compared the excretion characteristics of EtS with that of ethanol and ethyl glucuronide (EtG). Urine samples were collected from healthy subjects after a single ethanol dose, and also selected from routine clinical samples. Simultaneous analysis of EtS and ...
journal_title：Journal of analytical toxicology
pub_type： 杂志文章
doi：10.1093/jat/29.5.270
更新日期：2005-07-01 00:00:00
