Abstract:
:Buprenorphine, which is used in France as a substitution drug for opioid addiction, is widely abused, and several fatal cases have been reported. In order to confirm a recent intoxication or to establish retrospectively chronic abuse, a simple and reliable gas chromatographic-mass spectrometric method was developed and validated for quantitation of buprenorphine and its active metabolite norbuprenorphine in urine and hair. Two milliliters of urine or 50 mg of pulverized hair was submitted to a pretreatment (enzymatic hydrolysis for urine and decontamination with dichloromethane followed by incubation in 0.1 M HCI for hair). Buprenorphine-d4 was chosen as the internal standard. Selective solid-phase extraction with Bond Elut Certify columns provided recoveries higher than 85% for urine and 43% for hair. By using a mixture of MSTFA/TMSIM/TMCS (100:2:5), buprenorphine and norbuprenorphine produced stable silylated derivatives. The detection was carried out with a quadrupole mass detector working in El selected ion monitoring mode. Ions at m/z 450 and 468 were chosen for the quantitation of buprenorphine and norbuprenorphine, respectively (m/z 454 was used for the internal standard). Limits of quantitation were 0.25 and 0.20 ng/mL, respectively, for buprenorphine and norbuprenorphine in urine and 0.005 ng/mg for the two compounds in hair. Calibration curves were linear from 0 to 50 ng/mL in urine and from 0 to 0.4 ng/mg in hair. Between-day and within-day precisions were less than 8.4% in hair and 6.1% in urine for both molecules in all cases. This method was applied to urine and hair samples collected from patients in a withdrawal treatment program and demonstrated its good applicability in routine analysis and its benefit for clinicians. This technique, which requires instruments already available to many toxicology laboratories, offers an attractive alternative to more sophisticated techniques.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Vincent F,Bessard J,Vacheron J,Mallaret M,Bessard Gdoi
10.1093/jat/23.4.270keywords:
subject
Has Abstractpub_date
1999-07-01 00:00:00pages
270-9issue
4eissn
0146-4760issn
1945-2403journal_volume
23pub_type
杂志文章abstract::A method is described for the simultaneous determination of the ratio of l- and d-enantiomers of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDEA) in urine. The assay uses liquid-liquid extraction followed by deriv...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/23.6.518
更新日期:1999-10-01 00:00:00
abstract::Cyclizine is an antihistamine with sedative effect used to treat motion sickness. A few studies have reported on cyclizine abuse among teenagers, and cyclizine abuse has been reported among opioid dependants receiving methadone, with the combination having been reported to produce strong psychoactive effects. Few repo...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkt100
更新日期:2014-03-01 00:00:00
abstract::Analysis of drugs in hair differs from their analysis in other tissues due to the extended detection window, as well as the opportunity that segmental hair analysis offers for the detection of changes in drug intake over time. The antipsychotic drug chlorprothixene is widely used, but few reports exist on chlorprothix...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bky038
更新日期:2018-11-01 00:00:00
abstract::A relatively simple procedure for the determination of carboxyhemoglobin percent saturation in postmortem samples is described. The procedure involves a quick and accurate method for calibration of the gas chromatograph to carbon monoxide. The mixed gases are extracted from blood prior to introduction into the gas chr...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/4.4.181
更新日期:1980-07-01 00:00:00
abstract::Bisphenol S (BPS) has been detected in personal care products, water, food and indoor house dust, demonstrating the potential for human exposure. Due to limited data to characterize the hazard of BPS, the National Toxicology Program (NTP) is investigating the toxicity of BPS in rodent models. Generating systemic expos...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkab008
更新日期:2021-01-09 00:00:00
abstract::Urine specimens containing 11 common beta blockers were processed using solid-phase extraction technology to extract the drugs from the urine matrix, then converted to their cyclic methaneboronates by treatment with methaneboronic acid in ethyl acetate. The compounds tested included acebutolol, atenolol, alprenolol, b...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/22.2.135
更新日期:1998-03-01 00:00:00
abstract::Four kinds of volatile anesthetics (halothane, enflurane, isoflurane, and sevoflurane) that were dissolved in 3 microL of experimental plasma samples were examined by the pulse-heating gas chromatographic-mass spectrometric method, and this approach was found to be reliable for qualitative and quantitative analysis. T...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/19.2.115
更新日期:1995-03-01 00:00:00
abstract::A rapid and simple liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of L-ephedrine, pseudoephedrine, and caffeine in male Fisher-344 rat plasma at nanogram-per-milliliter concentrations for use in support of toxicology studies. Only 25 μL of plasma is...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/anatox/35.6.341
更新日期:2011-07-01 00:00:00
abstract::First synthesized in 1970, propafenone is a frequently used 1C antiarrhythmic drug metabolized into two major metabolites, 5-hydroxypropafenone and norpropafenone. Paradoxically, fatal intoxication is rarely described, and only six cases have been reported in the literature. We report our experience with two patients ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/27.8.595
更新日期:2003-11-01 00:00:00
abstract::A method for the detection and quantitation of methamphetamine and its major metabolite in hair, nails, sweat, and saliva from habitual users of methamphetamine by mass fragmentography has been developed. Hair and nail samples were washed with water and methanol to remove the external contamination, processed with 0.6...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/13.3.176
更新日期:1989-05-01 00:00:00
abstract::Caffeine is the most widely consumed drug in the world with coffee representing a major source of intake. Despite widespread availability, various medical conditions necessitate caffeine-restricted diets. Patients on certain prescription medications are advised to discontinue caffeine intake. Such admonition has impli...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/30.8.611
更新日期:2006-10-01 00:00:00
abstract::Hydrocodone in combination with acetaminophen is commonly used to control moderate pain and is metabolized by cytochrome P4502D6 to form the active metabolite, hydromorphone. The purpose of this study was to determine the metabolic relationship and variability between hydrocodone and its conversion to hydromorphone us...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bks019
更新日期:2012-05-01 00:00:00
abstract::Sixteen apparently intact procainamide tablets, in varied stages of drug depletion, were found distributed throughout the gastrointestinal tract of a man who died of arteriosclerotic heart disease with pulmonary complications. The tablets were found to contain procainamide by a thin layer chromatography system with ca...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/8.5.244
更新日期:1984-09-01 00:00:00
abstract::In this study, we investigated the effectiveness of the Roche Kinetic Interaction of Microparticles in Solution (KIMS) screening assay for cannabinoid metabolites. Urine specimens (N = 1689) were collected during elimination of cannabinoids from 25 subjects with a history of marijuana use. Specimens were analyzed conc...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/25.7.559
更新日期:2001-10-01 00:00:00
abstract::We report the validation of a quantitative method for paraquat in plasma and urine using high-performance liquid chromatography (HPLC) with ultraviolet detection (260 nm). Furthermore, we illustrate the use of this method in the clinic (over five years), in conjunction with a qualitative urine paraquat screen. Urine o...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/25.6.456
更新日期:2001-09-01 00:00:00
abstract::An automated column-switching liquid chromatographic-atmospheric pressure chemical ionization-mass spectrometric (LC-APCI-MS) method for the simultaneous determination of frequently encountered benzodiazepine hypnotics and their relevant metabolites in urine has been established. A column-switching online solid-phase ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/26.2.87
更新日期:2002-03-01 00:00:00
abstract::The efficiency of ethanolamine and potassium hydroxide (KOH) as trapping agents for CO2 was determined using a flow of 500 mL/min through CO2 absorption towers containing trapping solutions. Radiolabeled 14CO2 was produced by acidification of suspensions containing Ba14CO3. Both 100% ethanolamine and 5M ethanolamine i...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/10.1.24
更新日期:1986-01-01 00:00:00
abstract::A high performance liquid chromatographic method is reported, which incorporates three internal standards (I-cinchonidine, N-propylprocainamide, and para-chlorodisopyramide) for the simultaneous quantitation of four commonly prescribed antiarrhythmic drugs: quinidine, procainamide, N-acetylprocainamide, and disopyrami...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/7.2.65
更新日期:1983-03-01 00:00:00
abstract::The National Institute of Standards and Technology (formerly the National Bureau of Standards), in cooperation with the College of American Pathologists (CAP), has certified the concentrations of phencyclidine (PCP) in two new reference materials (RMs). One of these materials is Standard Reference Material (SRM) 1511,...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/20.1.43
更新日期:1996-01-01 00:00:00
abstract::Continuing our previous studies analyzing drugs of abuse in municipal wastewater, a method was developed for the analysis of opiates in wastewater samples using liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). Eight opiate drugs and metabolites were analyzed including codeine, hydrocodone, hydro...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkw022
更新日期:2016-06-01 00:00:00
abstract::The Abbott TDx Urine Amphetamine/Methamphetamine II fluorescence polarization immunoassay technique was applied to the determination of D-methamphetamine in hemolyzed whole blood. The assay was found to have 100% cross-reactivity with D-methamphetamine and only an 8% cross-reactivity with L-methamphetamine. Whole bloo...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/16.2.115
更新日期:1992-03-01 00:00:00
abstract::The selectivity of analytical methods used to determine ethanol in body fluids has always been an important concern in connection with chemical testing for intoxication. Allegations of false high breath-alcohol readings obtained because of the presence of volatile organic compounds (VOCs) other than ethanol is a well-...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/20.7.522
更新日期:1996-11-01 00:00:00
abstract::The aim of this study was to develop, optimize, and validate an analytical procedure to determine a group of eight amphetamines, amphetamine (AMP), ephedrine (EP), methcathinone (MTC), paramethoxyamphetamine (PMA), methamphetamine (MA), methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), and methyle...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/28.2.77
更新日期:2004-03-01 00:00:00
abstract::We devised a highly sensitive method for simultaneously determining methamphetamine (MA) and amphetamine (AP) enantiomers, desmethylselegiline (DMSG) and selegiline (SG), in human hair using a derivatization technique and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). M...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/30.4.232
更新日期:2006-05-01 00:00:00
abstract::Cannabinoids were extracted from serum with C18 SPE cartridges and analyzed as their trimethylsilyl (TMS) derivatives. A benchtop gas chromatography-tandem mass spectrometry (GC-MS-MS) system based on an ion trap with external ionization was used. Quantitation was done in relation to trideuterated internal standards i...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/24.5.359
更新日期:2000-07-01 00:00:00
abstract::The content, chemical form, and distribution of mercury and selenium in edible tissue were determined for several samples of fish and other marine animal organisms (mollusks, crustaceans and pods). For most fish samples, except Pacific blue marlin, 53 to 94% of the total mercury content was present as methylmercury, b...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/6.1.10
更新日期:1982-01-01 00:00:00
abstract::This manuscript reviews data available in the scientific literature relative to drug testing for the five mandated drug classes and circumstances other than abuse of the drug itself that could result in a positive test. For marijuana, passive inhalation, unknowing oral ingestion, and the use of Marinol are discussed. ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/19.6.450
更新日期:1995-10-01 00:00:00
abstract::3-Fluorophenmetrazine (3-FPM) is a stimulant-like novel psychoactive substance (NPS) and fluorinated analog of phenmetrazine that has recently appeared on the recreational drug market, with limited published information. Likewise, the synthetic opioid U-47700 has gained popularity among recreational drug users and is ...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/bkx060
更新日期:2017-11-01 00:00:00
abstract::Previous studies have shown that drug concentrations in blood can change during storage, especially at room temperature, but even labile drugs such as cocaine may be stable in dried blood spots (DBS). A new method has been developed for the analysis of hydrolytically labile drugs in blood spots on filter paper in orde...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/32.7.511
更新日期:2008-09-01 00:00:00
abstract::To study the disposition kinetics of methamphetamine (MAP), we have developed a sensitive high-performance liquid chromatographic (HPLC) assay to quantitate the enantiomers of MAP and its major metabolites, amphetamine (AP), p-hydroxymethamphetamine (p-OH-MAP), and p-hydroxyamphetamine (p-OH-AP), the latter two of whi...
journal_title:Journal of analytical toxicology
pub_type: 杂志文章
doi:10.1093/jat/19.3.139
更新日期:1995-05-01 00:00:00