Abstract:
:A high-performance liquid chromatographic method has been developed for the simultaneous analysis of eight frequently used benzodiazepines and some of their metabolites in postmortem samples. The method includes a liquid-liquid extraction with n-hexane-ethyl acetate (70:30, v/v), gradient elution of a C8 reversed-phase column with an alkaline water-methanol eluent free of buffer salts, and photodiode array detection. This allows rapid detection, a purity check, identification, and quantitation of the eluting peaks. Recovery and linearity data are good for the chosen parent compounds. The validated procedure has been applied routinely in forensic toxicological analyses. The elimination of the buffer from the eluent results in a robust procedure, free of technical problems and long rinsing periods.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Lambert WE,Meyer E,Xue-Ping Y,De Leenheer APdoi
10.1093/jat/19.1.35subject
Has Abstractpub_date
1995-01-01 00:00:00pages
35-40issue
1eissn
0146-4760issn
1945-2403journal_volume
19pub_type
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