Abstract：
:Cells of multicellular organisms need to adopt specific morphologies. However, the molecular mechanisms bringing about membrane topology changes are far from understood-mainly because knowledge of membrane-shaping proteins that can promote local membrane curvatures is still limited. Our analyses unveiled that several members of a large, previously unrecognised protein family, which we termed N-Ank proteins, use a combination of their ankyrin repeat array and an amino (N)-terminal amphipathic helix to bind and shape membranes. Consistently, functional analyses revealed that the N-Ank protein ankycorbin (NORPEG/RAI14), which was exemplarily characterised further, plays an important, ankyrin repeat-based and N-terminal amphipathic helix-dependent role in early morphogenesis of neurons. This function furthermore required coiled coil-mediated self-assembly and manifested as ankycorbin nanodomains marked by protrusive membrane topologies. In summary, here, we unveil a class of powerful membrane shapers and thereby assign mechanistic and cell biological functions to the N-Ank protein superfamily.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Wolf D,Hofbrucker-MacKenzie SA,Izadi M,Seemann E,Steiniger F,Schwintzer L,Koch D,Kessels MM,Qualmann Bdoi
10.1038/s41556-019-0381-7subject
Has Abstractpub_date
2019-10-01 00:00:00pages
1191-1205issue
10eissn
1465-7392issn
1476-4679pii
10.1038/s41556-019-0381-7journal_volume
21pub_type
杂志文章abstract：:The most dangerous insults to the genome's integrity are those that break both strands of the DNA. Double-strand breaks can be repaired by homologous recombination; in this conserved mechanism, a global genomic homology search finds sequences similar to those near the break, and uses them as a template for DNA synthes...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2745
更新日期：2013-06-01 00:00:00
abstract：:The kinase AMPK, a sensor of cellular energy stress, has been shown to oppose the growth-promoting activity of YAP, the transcriptional co-activator downstream of the Hippo signalling pathway. This finding may help to explain why the antidiabetic drug metformin, for which AMPK is a key effector, is linked to cancer-pr...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3141
更新日期：2015-04-01 00:00:00
abstract：:Induced pluripotent stem cells (iPSCs) are generated via the expression of the transcription factors OCT4 (also known as POU5F1), SOX2, KLF4 and cMYC (OSKM) in somatic cells. In contrast to murine naive iPSCs, conventional human iPSCs are in a more developmentally advanced state called primed pluripotency. Here, we re...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0254-5
更新日期：2019-02-01 00:00:00
abstract：:Primordial germ cell (PGC) development in the human fetus remains relatively uncharted. A new study suggests that epigenetic reprogramming and sex differentiation in human PGCs occur asynchronously over an extended time period. This finding raises questions and implications for in vitro PGC differentiation. ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb2665
更新日期：2013-01-01 00:00:00
abstract：:For accurate segregation of chromosomes during cell division, microtubule fibres must attach sister kinetochores to opposite poles of the mitotic spindle (bi-orientation). Aurora kinases are linked to oncogenesis and have been implicated in the regulation of chromosome-microtubule attachments. Although loss of Aurora ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1102
更新日期：2004-03-01 00:00:00
abstract：:Cell division is fundamental for all organisms. Here we report a genome-scale RNA-mediated interference screen in HeLa cells designed to identify human genes that are important for cell division. We have used a library of endoribonuclease-prepared short interfering RNAs for gene silencing and have used DNA content ana...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1659
更新日期：2007-12-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature cell biology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41556-020-0479-y
更新日期：2020-03-01 00:00:00
abstract：:Obesity occurs when excess energy accumulates in white adipose tissue (WAT), whereas brown adipose tissue (BAT), specialized for energy expenditure through thermogenesis, potently counteracts obesity. Factors that induce brown adipocyte commitment and energy expenditure would be a promising defence against adiposity. ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2867
更新日期：2013-12-01 00:00:00
abstract：:Cellular senescence is a stable proliferation arrest induced by triggers such as short telomeres, activated oncogenes and genotoxic stress. Two studies show that cellular senescence induced by genotoxic stress depends on chronic DNA-damage signalling from irreparable damage to telomeres. Hence, dysfunctional or damage...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb2476
更新日期：2012-04-02 00:00:00
abstract：:Most invasive solid tumours display predominantly collective invasion, in which groups of cells invade the peritumoral stroma while maintaining cell-cell contacts. As the concepts and experimental models for functional analysis of collective cancer cell invasion are rapidly developing, we propose a framework for addre...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb2548
更新日期：2012-08-01 00:00:00
abstract：:BACE1 activity is significantly increased in the brains of Alzheimer's disease patients, potentially contributing to neurodegeneration. The voltage-gated sodium channel (Na(v)1) beta2-subunit (beta2), a type I membrane protein that covalently binds to Na(v)1 alpha-subunits, is a substrate for BACE1 and gamma-secretase...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1602
更新日期：2007-07-01 00:00:00
abstract：:In plants, cell shape is defined by the cell wall, and changes in cell shape and size are dictated by modification of existing cell walls and deposition of newly synthesized cell-wall material. In root hairs, expansion occurs by a process called tip growth, which is shared by root hairs, pollen tubes and fungal hyphae...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2294
更新日期：2011-07-17 00:00:00
abstract：:The transition from cellular quiescence (G0) into S phase is regulated by the mitogenic-activation of D-type cyclins and cyclin-dependent kinases (Cdks), the sequestration of the Cdk inhibitors (CDKIs), p21 and p27, and the hyperphosphorylation of Rb with release of E2F transcription factors. However, fibroblasts that...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1548
更新日期：2007-03-01 00:00:00
abstract：:The function and regulation of repetitive DNA, the 'dark matter' of the genome, is still only rudimentarily understood. Now a study investigating DNA replication of repetitive centromeric chromosome segments has started to expose a fascinating replication program that involves suppression of ATR signalling, in particu...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3367
更新日期：2016-05-27 00:00:00
abstract：:Small RNAs generated at DNA break sites are implicated in mammalian DNA repair. Now, a study shows that following the formation of DNA double-strand breaks, bidirectional transcription events adjacent to the break generate small RNAs that trigger the DNA damage response by local RNA:RNA interactions. ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3645
更新日期：2017-11-29 00:00:00
abstract：:Two forms of genetic instability have been described in colorectal cancer: microsatellite instability and chromosomal instability. Microsatellite instability results from mutations in mismatch repair genes; chromosomal instability is the hallmark of many colorectal cancers, although it is not completely understood at ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35070129
更新日期：2001-04-01 00:00:00
abstract：:Centromeres are specialized chromosomal domains that direct kinetochore assembly during mitosis. CENP-A (centromere protein A), a histone H3-variant present exclusively in centromeric nucleosomes, is thought to function as an epigenetic mark that specifies centromere identity. Here we identify the essential centromere...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1899
更新日期：2009-07-01 00:00:00
abstract：:The cell division axis determines the future positions of daughter cells and is therefore critical for cell fate. The positioning of the division axis has been mostly studied in systems such as embryos or yeasts, in which cell shape is well defined. In these cases, cell shape anisotropy and cell polarity affect spindl...
journal_title：Nature cell biology
pub_type： 信件
doi：10.1038/ncb1307
更新日期：2005-10-01 00:00:00
abstract：:Lingual keratinized epithelial cells, which constitute the filiform papillae of the tongue, have one of the most rapid tissue turnover rates in the mammalian body and are thought to be the source of squamous cell carcinoma of the tongue. However, the mechanism of tissue maintenance and regeneration is largely unknown ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2719
更新日期：2013-05-01 00:00:00
abstract：:MAPKAP kinase 2 (MK2) is one of several kinases that are regulated through direct phosphorylation by p38 MAP kinase. By introducing a targeted mutation into the mouse MK2 gene, we have determined the physiological function of MK2 in vivo. Mice that lack MK2 show increased stress resistance and survive LPS-induced endo...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/10061
更新日期：1999-06-01 00:00:00
abstract：:Cancer-associated fibroblasts (CAFs) promote tumour invasion and metastasis. We show that CAFs exert a physical force on cancer cells that enables their collective invasion. Force transmission is mediated by a heterophilic adhesion involving N-cadherin at the CAF membrane and E-cadherin at the cancer cell membrane. Th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3478
更新日期：2017-03-01 00:00:00
abstract：:A key step in nutrient sensing is activation of the master growth regulator, mTORC1 kinase, on the lysosomal membrane. Nutrients enable mTORC1 scaffolding by a complex composed of the Rag GTPases (Rags) and Ragulator, but the underlying mechanism of mTORC1 capture is poorly understood. Combining dynamic imaging in cel...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0148-6
更新日期：2018-09-01 00:00:00
abstract：:During apoptosis, dying cells are swiftly removed by phagocytes. It is not fully understood how apoptotic cells are recognized by phagocytes. Here we report the identification and characterization of the Caenorhabditis elegans ttr-52 gene, which encodes a transthyretin-like protein and is required for efficient cell c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2068
更新日期：2010-07-01 00:00:00
abstract：:Extracellular signal regulated kinases (ERKs) represent a signalling hub in many physiological responses and have pivotal functions in cell proliferation, differentiation, development and death, as well as in synaptic plasticity. Mitogen-activated protein kinase phosphatases (MKPs) selectively inactivate ERKs by depho...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1447
更新日期：2006-08-01 00:00:00
abstract：:Correct placement and orientation of the mitotic spindle is essential for segregation of localized components and positioning of daughter cells. Although these processes are important in many cells, few factors that regulate spindle placement are known. Previous work has shown that GPB-1, the Gbeta subunit of a hetero...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35060092
更新日期：2001-03-01 00:00:00
abstract：:A surprising functional association between TRPM3, a mysterious member of the family of transient receptor potential (TRP) cation channels, and the sulphated version of pregnenolone, 'mother' of all steroid hormones, has been identified. ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb1208-1383
更新日期：2008-12-01 00:00:00
abstract：:Canonical Wnt signalling has been implicated in mouse and human embryonic stem cell (ESC) maintenance; however, its requirement is controversial. β-catenin is the key component in this highly conserved Wnt pathway, acting as a transcriptional transactivator. However, β-catenin has additional roles at the plasma membra...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2260
更新日期：2011-06-19 00:00:00
abstract：:In response to DNA damage, eukaryotic cells use a system of checkpoint controls to delay cell-cycle progression. Checkpoint delays provide time for repair of damaged DNA before its replication in S phase and before segregation of chromatids in M phase. The Cds1 (Chk2) tumour-suppressor protein has been implicated in c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35036406
更新日期：2000-10-01 00:00:00
abstract：:The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane components. Here, simultaneous observations of quantum dot-labelled FcepsilonRI motion and GFP-tagged actin dynamics provide direct evidence that actin filament bundles define micron-sized domains that confine mobile receptors. Dyna...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1755
更新日期：2008-08-01 00:00:00
abstract：:A number of key regulators of mouse embryonic stem (ES) cell identity, including the transcription factor Nanog, show strong expression fluctuations at the single-cell level. The molecular basis for these fluctuations is unknown. Here we used a genetic complementation strategy to investigate expression changes during ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2603
更新日期：2012-11-01 00:00:00
