A TRP channel-steroid marriage.

abstract：:The most dangerous insults to the genome's integrity are those that break both strands of the DNA. Double-strand breaks can be repaired by homologous recombination; in this conserved mechanism, a global genomic homology search finds sequences similar to those near the break, and uses them as a template for DNA synthes...

journal_title：Nature cell biology

authors： Agmon N,Liefshitz B,Zimmer C,Fabre E,Kupiec M

更新日期：2013-06-01 00:00:00

abstract：:We describe a genome-wide gain-of-function screen for regulators of NF-kappaB, and identify Rap1 (Trf2IP), as an essential modulator of NF-kappaB-mediated pathways. NF-kappaB is induced by ectopic expression of Rap1, whereas its activity is inhibited by Rap1 depletion. In addition to localizing on telomeres, mammalian...

journal_title：Nature cell biology

authors： Teo H,Ghosh S,Luesch H,Ghosh A,Wong ET,Malik N,Orth A,de Jesus P,Perry AS,Oliver JD,Tran NL,Speiser LJ,Wong M,Saez E,Schultz P,Chanda SK,Verma IM,Tergaonkar V

更新日期：2010-08-01 00:00:00

abstract：:Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose act...

journal_title：Nature cell biology

authors： Alvarado-Kristensson M,Rodríguez MJ,Silió V,Valpuesta JM,Carrera AC

更新日期：2009-09-01 00:00:00

abstract：:During interphase, centrosomes are held together by a proteinaceous linker that connects the proximal ends of the mother and daughter centriole. This linker is disassembled at the onset of mitosis in a process known as centrosome disjunction, thereby facilitating centrosome separation and bipolar spindle formation. Th...

journal_title：Nature cell biology

authors： Mardin BR,Lange C,Baxter JE,Hardy T,Scholz SR,Fry AM,Schiebel E

更新日期：2010-12-01 00:00:00

abstract：:The AP-1 transcription factor c-Jun is essential for cellular proliferation in many cell types, but the molecular link between growth factors and c-Jun activation has been enigmatic. In this study we identify a previously uncharacterized RING-domain-containing protein, RACO-1 (RING domain AP-1 co-activator-1), as a c-...

journal_title：Nature cell biology

authors： Davies CC,Chakraborty A,Cipriani F,Haigh K,Haigh JJ,Behrens A

更新日期：2010-10-01 00:00:00

abstract：:Influx of Ca(2+) through store-operated Ca(2+) channels (SOCs) is a central component of receptor-evoked Ca(2+) signals. Orai channels are SOCs that are gated by STIM1, a Ca(2+) sensor located in the ER but how it gates and regulates the Orai channels is unknown. Here, we report the molecular basis for gating of Orais...

journal_title：Nature cell biology

authors： Yuan JP,Zeng W,Dorwart MR,Choi YJ,Worley PF,Muallem S

更新日期：2009-03-01 00:00:00

abstract：:Chromophore-assisted laser inactivation (CALI) is a light-mediated technique used to selectively inactivate proteins within cells. Here, we demonstrate that GFP can be used as a CALI reagent to locally inactivate proteins in living cells. We show that focused laser irradiation of EGFP-alpha-actinin expressed in Swiss ...

journal_title：Nature cell biology

authors： Rajfur Z,Roy P,Otey C,Romer L,Jacobson K

更新日期：2002-04-01 00:00:00

abstract：:Pluripotent stem cells (PSCs) may provide a potential source of haematopoietic stem/progenitor cells (HSPCs) for transplantation; however, unknown molecular barriers prevent the self-renewal of PSC-HSPCs. Using two-step differentiation, human embryonic stem cells (hESCs) differentiated in vitro into multipotent haemat...

journal_title：Nature cell biology

authors： Dou DR,Calvanese V,Sierra MI,Nguyen AT,Minasian A,Saarikoski P,Sasidharan R,Ramirez CM,Zack JA,Crooks GM,Galic Z,Mikkola HK

更新日期：2016-06-01 00:00:00

abstract：:Although myosin II filaments are known to exist in non-muscle cells, their dynamics and organization are incompletely understood. Here, we combined structured illumination microscopy with pharmacological and genetic perturbations, to study the process of actomyosin cytoskeleton self-organization into arcs and stress f...

journal_title：Nature cell biology

authors： Hu S,Dasbiswas K,Guo Z,Tee YH,Thiagarajan V,Hersen P,Chew TL,Safran SA,Zaidel-Bar R,Bershadsky AD

更新日期：2017-02-01 00:00:00

abstract：:The vertebrate neural crest arises at the border of the neural plate during early stages of nervous system development; however, little is known about the molecular mechanisms underlying neural crest formation. Here we identify a secreted protein, Noelin-1, which has the ability to prolong neural crest production. Noe...

journal_title：Nature cell biology

authors： Barembaum M,Moreno TA,LaBonne C,Sechrist J,Bronner-Fraser M

更新日期：2000-04-01 00:00:00

abstract：:Phosphoinositides (PIs) undergo phosphorylation/dephosphorylation cycles through organelle-specific PI kinases and PI phosphatases that lead to distinct subcellular distributions of the individual PI species. Specific PIs control the correct timing and location of many trafficking events. Their ultimate mode of action...

journal_title：Nature cell biology

authors： De Matteis MA,Godi A

更新日期：2004-06-01 00:00:00

abstract：:Research on genome stability and integrity now extends far beyond the biochemistry of DNA repair to encompass signal transduction pathways that span numerous aspects of cellular life. Derailed genomic integrity pathways can result in debilitating genetic disorders, premature ageing, predisposition to cancer and degene...

journal_title：Nature cell biology

authors： Shiloh Y,Lehmann AR

更新日期：2004-10-01 00:00:00

abstract：:Here we show that human Zeste White 10 (Zw10) and Rough deal (Rod) are new components of the mitotic checkpoint, as cells lacking these proteins at kinetochores fail to arrest in mitosis when exposed to microtubule inhibitors. Checkpoint failure and premature mitotic exit may explain why cells defective for hZw10 and ...

journal_title：Nature cell biology

authors： Chan GK,Jablonski SA,Starr DA,Goldberg ML,Yen TJ

更新日期：2000-12-01 00:00:00

abstract：:The molecular requirements and morphology of migrating cells can vary depending on matrix geometry; therefore, predicting the optimal migration strategy or the effect of experimental perturbation is difficult. We present a model of cell motility that encompasses actin-polymerization-based protrusions, actomyosin contr...

journal_title：Nature cell biology

authors： Tozluoğlu M,Tournier AL,Jenkins RP,Hooper S,Bates PA,Sahai E

更新日期：2013-07-01 00:00:00

abstract：:Wiskott-Aldrich syndrome protein (WASP) and N-WASP have emerged as key proteins connecting signalling cascades to actin polymerization. Here we show that the amino-terminal WH1 domain, and not the polyproline-rich region, of N-WASP is responsible for its recruitment to sites of actin polymerization during Cdc42-indepe...

journal_title：Nature cell biology

authors： Moreau V,Frischknecht F,Reckmann I,Vincentelli R,Rabut G,Stewart D,Way M

更新日期：2000-07-01 00:00:00

abstract：:It is now well established that stromal interaction molecule 1 (STIM1) is the calcium sensor of endoplasmic reticulum stores required to activate store-operated calcium entry (SOC) channels at the surface of non-excitable cells. However, little is known about STIM1 in excitable cells, such as striated muscle, where th...

journal_title：Nature cell biology

authors： Stiber J,Hawkins A,Zhang ZS,Wang S,Burch J,Graham V,Ward CC,Seth M,Finch E,Malouf N,Williams RS,Eu JP,Rosenberg P

更新日期：2008-06-01 00:00:00

abstract：:We have identified Rab10 as an ER-specific Rab GTPase that regulates ER structure and dynamics. We show that Rab10 localizes to the ER and to dynamic ER-associated structures that track along microtubules and mark the position of new ER tubule growth. Rab10 depletion or expression of a Rab10 GDP-locked mutant alters E...

journal_title：Nature cell biology

authors： English AR,Voeltz GK

更新日期：2013-02-01 00:00:00

abstract：:Formation of a stable lamellipodium at the front of migrating cells requires localization of Rac activation to the leading edge. Restriction of alpha4 integrin phosphorylation to the leading edge limits the interaction of alpha4 with paxillin to the sides and rear of a migrating cell. The alpha4-paxillin complex inhib...

journal_title：Nature cell biology

authors： Nishiya N,Kiosses WB,Han J,Ginsberg MH

更新日期：2005-04-01 00:00:00

abstract：:The endoplasmic reticulum (ER), a network of membranous sheets and pipes, supports functions encompassing biogenesis of secretory proteins and delivery of functional solutes throughout the cell1,2. Molecular mobility through the ER network enables these functionalities, but diffusion alone is not sufficient to explain...

journal_title：Nature cell biology

authors： Holcman D,Parutto P,Chambers JE,Fantham M,Young LJ,Marciniak SJ,Kaminski CF,Ron D,Avezov E

更新日期：2018-10-01 00:00:00

abstract：:In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. ...

journal_title：Nature cell biology

authors： Okada M,Cheeseman IM,Hori T,Okawa K,McLeod IX,Yates JR 3rd,Desai A,Fukagawa T

更新日期：2006-05-01 00:00:00

abstract：:RNA interference has become an indispensable tool for loss-of-function studies across eukaryotes. By enabling stable and reversible gene silencing, shRNAs provide a means to study long-term phenotypes, perform pool-based forward genetic screens and examine the consequences of temporary target inhibition in vivo. Howev...

journal_title：Nature cell biology

authors： Fellmann C,Lowe SW

更新日期：2014-01-01 00:00:00

abstract：:Phosphorylation networks intimately regulate mechanisms of response to therapies. Mapping the phospho-catalytic profile of kinases in cells or tissues remains a challenge. Here, we introduce a practical high-throughput system to measure the enzymatic activity of kinases using biological peptide targets as phospho-sens...

journal_title：Nature cell biology

authors： Coppé JP,Mori M,Pan B,Yau C,Wolf DM,Ruiz-Saenz A,Brunen D,Prahallad A,Cornelissen-Steijger P,Kemper K,Posch C,Wang C,Dreyer CA,Krijgsman O,Lee PRE,Chen Z,Peeper DS,Moasser MM,Bernards R,van 't Veer LJ

更新日期：2019-06-01 00:00:00

abstract：:Enhancer of zeste homologue 2 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and catalyses the trimethylation of histone H3 on Lys 27 (H3K27), which represses gene transcription. EZH2 enhances cancer-cell invasiveness and regulates stem cell differentiation. Here, we demonstrate that EZH2 can ...

journal_title：Nature cell biology

authors： Wei Y,Chen YH,Li LY,Lang J,Yeh SP,Shi B,Yang CC,Yang JY,Lin CY,Lai CC,Hung MC

更新日期：2011-01-01 00:00:00

abstract：:While nuclear lamina abnormalities are hallmarks of human diseases, their interplay with epigenetic regulators and precise epigenetic landscape remain poorly understood. Here, we show that loss of the lysine acetyltransferase MOF or its associated NSL-complex members KANSL2 or KANSL3 leads to a stochastic accumulation...

journal_title：Nature cell biology

authors： Karoutas A,Szymanski W,Rausch T,Guhathakurta S,Rog-Zielinska EA,Peyronnet R,Seyfferth J,Chen HR,de Leeuw R,Herquel B,Kimura H,Mittler G,Kohl P,Medalia O,Korbel JO,Akhtar A

更新日期：2019-10-01 00:00:00

abstract：:Metaphase spindles are microtubule-based structures that use a multitude of proteins to modulate their morphology and function. Today, we understand many details of microtubule assembly, the role of microtubule-associated proteins, and the action of molecular motors. Ultimately, the challenge remains to understand how...

journal_title：Nature cell biology

authors： Reber SB,Baumgart J,Widlund PO,Pozniakovsky A,Howard J,Hyman AA,Jülicher F

更新日期：2013-09-01 00:00:00

abstract：:The directional movement of cells in a gradient of external stimulus is termed chemotaxis and is important in many aspects of development and differentiated cell function. Phophoinositide 3-kinases (PI(3)Ks) are thought to have critical roles within the gradient-sensing machinery of a variety of highly motile cells, s...

journal_title：Nature cell biology

authors： Ferguson GJ,Milne L,Kulkarni S,Sasaki T,Walker S,Andrews S,Crabbe T,Finan P,Jones G,Jackson S,Camps M,Rommel C,Wymann M,Hirsch E,Hawkins P,Stephens L

更新日期：2007-01-01 00:00:00

abstract：:During cell division, many animal cells transform into a spherical shape and assemble a contractile ring composed of actin filaments and myosin motors at the equator to separate the cell body into two. Although actomyosin regulatory proteins are spatio-temporally controlled during cytokinesis, the direct contribution ...

journal_title：Nature cell biology

authors： Miyazaki M,Chiba M,Eguchi H,Ohki T,Ishiwata S

更新日期：2015-04-01 00:00:00

abstract：:The physical characteristics of tumours are intricately linked to the tumour phenotype and difficulties during treatment. Many factors contribute to the increased stiffness of tumours; from increased matrix deposition, matrix remodelling by forces from cancer cells and stromal fibroblasts, matrix crosslinking, increas...

journal_title：Nature cell biology

authors： Mohammadi H,Sahai E

更新日期：2018-07-01 00:00:00

abstract：:Protein degradation in eukaryotes often requires the ubiquitin-selective chaperone p97 for substrate recruitment and ubiquitin-chain assembly. However, the physiological relevance of p97, and its role in developmental processes, remain unclear. Here, we discover an unanticipated function for CDC-48/p97 in myosin assem...

journal_title：Nature cell biology

authors： Janiesch PC,Kim J,Mouysset J,Barikbin R,Lochmüller H,Cassata G,Krause S,Hoppe T

更新日期：2007-04-01 00:00:00

abstract：:Small RNAs generated at DNA break sites are implicated in mammalian DNA repair. Now, a study shows that following the formation of DNA double-strand breaks, bidirectional transcription events adjacent to the break generate small RNAs that trigger the DNA damage response by local RNA:RNA interactions. ...

journal_title：Nature cell biology

authors： Storici F,Tichon AE

更新日期：2017-11-29 00:00:00