Abstract：
:Tight junctions contribute to epithelial barrier function by selectively regulating the quantity and type of molecules that cross the paracellular barrier. Experimental approaches to evaluate the effectiveness of tight junctions are typically global, tissue-scale measures. Here, we introduce Zinc-based Ultrasensitive Microscopic Barrier Assay (ZnUMBA), which we used in Xenopus laevis embryos to visualize short-lived, local breaches in epithelial barrier function. These breaches, or leaks, occur as cell boundaries elongate, correspond to visible breaks in the tight junction, and are followed by transient localized Rho activation, or Rho flares. We discovered that Rho flares restore barrier function by driving concentration of tight junction proteins through actin polymerization and ROCK-mediated localized contraction of the cell boundary. We conclude that Rho flares constitute a damage control mechanism that reinstates barrier function when tight junctions become locally compromised because of normally occurring changes in cell shape and tissue tension.
journal_name
Dev Celljournal_title
Developmental cellauthors
Stephenson RE,Higashi T,Erofeev IS,Arnold TR,Leda M,Goryachev AB,Miller ALdoi
10.1016/j.devcel.2019.01.016subject
Has Abstractpub_date
2019-02-25 00:00:00pages
445-459.e5issue
4eissn
1534-5807issn
1878-1551pii
S1534-5807(19)30044-9journal_volume
48pub_type
杂志文章abstract：:During embryogenesis, melanoblasts proliferate and migrate ventrally through the developing dermis and epidermis as single cells. Targeted deletion of Rac1 in melanoblasts during embryogenesis causes defects in migration, cell-cycle progression, and cytokinesis. Rac1 null cells migrate markedly less efficiently, but s...
journal_title：Developmental cell
pub_type： 杂志文章
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abstract：:During the first five rounds of cell division in the mouse embryo, spindles assemble in the absence of centrioles. Spindle formation initiates around chromosomes, but the microtubule nucleating process remains unclear. Here we demonstrate that Plk4, a protein kinase known as a master regulator of centriole formation, ...
journal_title：Developmental cell
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abstract：:Positive autoregulation is an effective mechanism for the long-term maintenance of a transcription factor's expression. This strategy is widely deployed in cell lineages, where the autoregulatory factor controls the activity of a battery of genes that constitute the differentiation program of a postmitotic cell type. ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2014.02.005
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abstract：:The conserved Hippo kinase pathway plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Whereas the function of the core kinase cascade, consisting of the serine/threonine kinases Hippo and Warts, in phosphorylating and thereby inactivating the transcri...
journal_title：Developmental cell
pub_type： 杂志文章
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abstract：:CLASPs are mammalian microtubule-stabilizing proteins that can mediate the interaction between distal microtubule ends and the cell cortex. Using mass spectrometry-based assays, we have identified two CLASP partners, LL5beta and ELKS. LL5beta and ELKS form a complex that colocalizes with CLASPs at the cortex of HeLa c...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2006.05.012
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abstract：:Microtubules are non-covalent mesoscale polymers central to the eukaryotic cytoskeleton. Microtubule structure, dynamics, and mechanics are modulated by a cell's choice of tubulin isoforms and post-translational modifications, a "tubulin code," which is thought to support the diverse morphology and dynamics of microtu...
journal_title：Developmental cell
pub_type： 杂志文章,评审
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abstract：:CLIPs are microtubule plus end-associated proteins that mediate interactions required for cell polarity and cell division. Here we demonstrate that budding yeast Bik1, unlike its human ortholog CLIP-170, is targeted to the microtubule plus end by a kinesin-dependent transport mechanism. Bik1 forms a complex with the k...
journal_title：Developmental cell
pub_type： 杂志文章
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abstract：:Pancreatic beta cells have been shown to be heterogeneous at multiple levels. However, spatially interrogating transcriptional heterogeneity in the intact tissue has been challenging. Here, we developed an optimized protocol for single-molecule transcript imaging in the intact pancreas and used it to identify a sub-po...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2018.11.001
更新日期：2019-01-07 00:00:00
abstract：:We have used Drosophila ovarian follicle stem cells (FSCs) to study how stem cells are regulated by external signals and draw three main conclusions. First, the spatial definition of supportive niche positions for FSCs depends on gradients of Hh and JAK-STAT pathway ligands, which emanate from opposite, distant sites....
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journal_title：Developmental cell
pub_type： 杂志文章,评审
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2007.06.010
更新日期：2007-08-01 00:00:00
abstract：:Centromeres are essential for accurate chromosome segregation and are marked by centromere protein A (CENP-A) nucleosomes. Mis-targeted CENP-A chromatin has been shown to seed centromeres at non-centromeric DNA. However, the requirements for such de novo centromere formation and transmission in vivo remain unknown. He...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2020.01.005
更新日期：2020-02-10 00:00:00
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journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2017.05.017
更新日期：2017-06-05 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2019.04.031
更新日期：2019-06-17 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2019.04.018
更新日期：2019-05-20 00:00:00
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journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2015.08.022
更新日期：2015-09-14 00:00:00
abstract：:A balance between Six2-dependent self-renewal and canonical Wnt signaling-directed commitment regulates mammalian nephrogenesis. Intersectional studies using chromatin immunoprecipitation and transcriptional profiling identified direct target genes shared by each pathway within nephron progenitors. Wnt4 and Fgf8 are e...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2012.07.008
更新日期：2012-09-11 00:00:00
abstract：:The generation and shedding of extracellular vesicles (EVs), including exosomes and microvesicles (MVs), by cells has emerged as a form of intercellular communication with important roles in several physiological processes and diseases such as cancer. These membrane-enclosed packets can transfer specific proteins, RNA...
journal_title：Developmental cell
pub_type： 杂志文章,评审
doi：10.1016/j.devcel.2016.04.019
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abstract：:We use the Dpp morphogen gradient in the Drosophila wing disc as a model to address the fundamental question of how a gradient of a growth factor can produce uniform growth. We first show that proper expression and subcellular localization of components in the Fat tumor-suppressor pathway, which have been argued to de...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2010.11.007
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journal_title：Developmental cell
pub_type： 评论,杂志文章
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abstract：:In eukaryotes, small RNAs (sRNAs) have key roles in development, gene expression regulation, and genome integrity maintenance. In ciliates, such as Paramecium, sRNAs form the heart of an epigenetic system that has evolved from core eukaryotic gene silencing components to selectively target DNA for deletion. In Paramec...
journal_title：Developmental cell
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doi：10.1016/j.devcel.2013.12.010
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journal_title：Developmental cell
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journal_title：Developmental cell
pub_type： 杂志文章
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更新日期：2019-07-01 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2016.02.010
更新日期：2016-03-07 00:00:00
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journal_title：Developmental cell
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doi：10.1016/j.devcel.2005.02.010
更新日期：2005-04-01 00:00:00
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journal_title：Developmental cell
pub_type： 评论,杂志文章
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更新日期：2007-11-01 00:00:00
abstract：:Manipulation of cellular identity in the laboratory is generally inefficient due to stochastic events, including epigenomic alterations. Recently in Science, Zuryn et al. (2014) demonstrate that the efficiency of a naturally occurring transdifferentiation event in Caenorhabditis elegans is ensured through stepwise epi...
journal_title：Developmental cell
pub_type： 评论,杂志文章
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journal_title：Developmental cell
pub_type： 杂志文章,评审
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更新日期：2019-05-20 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
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更新日期：2018-09-10 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2011.04.007
更新日期：2011-05-17 00:00:00
