Abstract:
:Dynamic rearrangements of the actin cytoskeleton play a key role in numerous cellular processes. In Drosophila, fusion between a muscle founder cell and a fusion competent myoblast (FCM) is mediated by an invasive, F-actin-enriched podosome-like structure (PLS). Here, we show that the dynamics of the PLS is controlled by Blown fuse (Blow), a cytoplasmic protein required for myoblast fusion but whose molecular function has been elusive. We demonstrate that Blow is an FCM-specific protein that colocalizes with WASP, WIP/Solitary, and the actin focus within the PLS. Biochemically, Blow modulates the stability of the WASP-WIP complex by competing with WASP for WIP binding, leading to a rapid exchange of WASP, WIP and G-actin within the PLS, which, in turn, actively invades the adjacent founder cell to promote fusion pore formation. These studies identify a regulatory protein that modulates the actin cytoskeletal dynamics by controlling the stability of the WASP-WIP complex.
journal_name
Dev Celljournal_title
Developmental cellauthors
Jin P,Duan R,Luo F,Zhang G,Hong SN,Chen EHdoi
10.1016/j.devcel.2011.04.007subject
Has Abstractpub_date
2011-05-17 00:00:00pages
623-38issue
5eissn
1534-5807issn
1878-1551pii
S1534-5807(11)00158-4journal_volume
20pub_type
杂志文章abstract::The Cubitus interruptus (Ci)/Gli family of transcription factors can be degraded either completely or partially from a full-length form (Ci155/Gli(FL)) to a truncated repressor (Ci75/Gli(R)) by proteasomes to mediate Hedgehog (Hh) signaling. The mechanism by which proteasomes distinguish ubiquitinated Ci/Gli to carry ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2013.05.006
更新日期:2013-06-24 00:00:00
abstract::Integrin expression and activity have been strongly correlated with developmental and pathological processes involving cell invasion through basement membranes. The role of integrins in mediating these invasions, however, remains unclear. Utilizing the genetically and visually accessible model of anchor cell (AC) inva...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2009.06.006
更新日期:2009-08-01 00:00:00
abstract::The faithful alignment of homologous chromosomes during meiotic prophase requires the coordination of DNA double-strand break (DSB) repair with large-scale chromosome reorganization. Here we identify the phosphatase PP4 (Pph3/Psy2) as a mediator of this process in Saccharomyces cerevisiae. In pp4 mutants, early stages...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2010.09.006
更新日期:2010-10-19 00:00:00
abstract::Cells that undergo epithelial-to-mesenchymal transitions (EMTs) commonly switch from expressing E-cadherin to N-cadherin. But why this occurs is not well understood. In the current issue of Developmental Cell, Scarpa et al. (2015) identify a reason: cadherin switching controls Rac signaling to determine how cell locom...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2015.08.002
更新日期:2015-08-24 00:00:00
abstract::Alzheimer's disease remains a formidable challenge for therapeutic management. In a recent report in Nature Structural and Molecular Biology, Cohen et al. (2015) present intriguing results showing that "designer" molecular chaperones may hold the key to an evolutionarily conserved program for management of toxic oligo...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2015.03.009
更新日期:2015-03-23 00:00:00
abstract::Pontin and reptin are conserved AAA+ ATPases identified in chromatin-remodeling complexes. In a recent issue of Cell, Venteicher et al. provide new insight into the function of pontin and reptin in telomerase biogenesis, which is important for cellular senescence, aging, and cancer. These unexpected findings have impl...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2008.03.018
更新日期:2008-04-01 00:00:00
abstract::CLIPs are microtubule plus end-associated proteins that mediate interactions required for cell polarity and cell division. Here we demonstrate that budding yeast Bik1, unlike its human ortholog CLIP-170, is targeted to the microtubule plus end by a kinesin-dependent transport mechanism. Bik1 forms a complex with the k...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2004.05.001
更新日期:2004-06-01 00:00:00
abstract::Sox2 expression marks neural and sensory primordia at various stages of development. A 50 kb genomic region of chicken Sox2 was isolated and scanned for enhancer activity utilizing embryo electroporation, resulting in identification of a battery of enhancers. Although Sox2 expression in the early embryonic CNS appears...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(03)00088-1
更新日期:2003-04-01 00:00:00
abstract::We have used Drosophila ovarian follicle stem cells (FSCs) to study how stem cells are regulated by external signals and draw three main conclusions. First, the spatial definition of supportive niche positions for FSCs depends on gradients of Hh and JAK-STAT pathway ligands, which emanate from opposite, distant sites....
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2012.09.010
更新日期:2012-10-16 00:00:00
abstract::The regulatory circuits that control the activities of the two distinct target of rapamycin (TOR) complexes, TORC1 and TORC2, and of Akt have been a focus of intense research in recent years. It has become increasingly evident that these regulatory circuits control some of the most fundamental aspects of metabolism, c...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2007.03.020
更新日期:2007-04-01 00:00:00
abstract::The eukaryotic nucleus has been the neglected child of cell biology. The "International Symposium on Functional Organization of the Nucleus" held in January on Awaji Island, Japan, highlighted recent work on nuclear organization and function. Emerging from this conference was a holistic view in which diverse chemical ...
journal_title:Developmental cell
pub_type:
doi:10.1016/j.devcel.2007.02.014
更新日期:2007-03-01 00:00:00
abstract::The binding of Hedgehog (Hh) to its receptor Patched causes derepression of Smoothened (Smo), resulting in the activation of the Hh pathway. Here, we show that Smo activation is dependent on the levels of the phospholipid phosphatidylinositol-4 phosphate (PI4P). Loss of STT4 kinase, which is required for the generatio...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2010.06.007
更新日期:2010-07-20 00:00:00
abstract::Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asym...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2008.06.002
更新日期:2008-08-01 00:00:00
abstract::Migrating fibroblasts undergo contact inhibition of locomotion (CIL), a process that was discovered five decades ago and still is not fully understood at the molecular level. We identify the Slit2-Robo4-srGAP2 signaling network as a key regulator of CIL in fibroblasts. CIL involves highly dynamic contact protrusions w...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2015.09.002
更新日期:2015-10-12 00:00:00
abstract::Neutrophils, in response to a chemoattractant gradient, undergo dynamic F-actin remodeling, a process important for their directional migration or chemotaxis. However, signaling mechanisms for chemoattractants to regulate the process are incompletely understood. Here, we characterized chemoattractant-activated signali...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.10.023
更新日期:2011-12-13 00:00:00
abstract::The connection between cell fate transitions and metabolic shifts is gaining momentum in the study of cell differentiation in embryonic development, adult stem cells, and cancer pathogenesis. Here, we explore how metabolic transitions influence post-translational modifications (PTMs), which play central roles in the a...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2020.06.035
更新日期:2020-07-20 00:00:00
abstract::Cell locomotion entails functional and structural cooperation between cell surface adhesion and the actin cytoskeleton. A new paper by DeMali et al. provides new insights into the link between actin assembly and integrin adhesion at the leading edges of migrating cells. ...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/s1534-5807(02)00374-x
更新日期:2002-12-01 00:00:00
abstract::Mammalian neurulation is completed when the dorsolateral neural folds bend inwards, their tips make adhesive contacts across the midline, and the epithelia remodel to create a closed neural tube. Two recent papers (one by Camerer et al. in this issue of Developmental Cell) demonstrate a vital role for protease-activat...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2010.01.002
更新日期:2010-01-19 00:00:00
abstract::Developmental growth requires coordination between the growth rates of individual tissues and organs. Here, we examine how Drosophila neuromuscular synapses grow to match the size of their target muscles. We show that changes in muscle growth driven by autonomous modulation of insulin receptor signaling produce corres...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2020.05.015
更新日期:2020-07-06 00:00:00
abstract::A central enigma in epigenetics is how epigenetic factors are guided to specific genomic sites for their function. Previously, we reported that a Piwi-piRNA complex associates with the piRNA-complementary site in the Drosophila genome and regulates its epigenetic state. Here, we report that Piwi-piRNA complexes bind t...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2013.01.023
更新日期:2013-03-11 00:00:00
abstract::The extracellular signals and corresponding receptors that align the mitotic spindle of symmetrically dividing cells within an epithelial sheet are largely unknown. In this issue of Developmental Cell, Xia et al. (2015) identify semaphorin-plexin signaling as a regulator of spindle orientation critical for kidney deve...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2015.04.012
更新日期:2015-05-04 00:00:00
abstract::In the field of breast biology, there is a growing appreciation for the "gatekeeping function" of basal cells during development and disease processes yet mechanisms regulating the generation of these cells are poorly understood. Here, we report that the proliferation of basal cells is controlled by SLIT/ROBO1 signali...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.05.012
更新日期:2011-06-14 00:00:00
abstract::Transmission of the human parasite Brugia malayi relies on the sustained production of larvae in blood. In this issue of Developmental Cell,Foray et al. (2018) use methods developed in the model nematode C. elegans to reveal how a symbiotic bacterium supports the female germ cell development underlying this massive fe...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.04.006
更新日期:2018-04-23 00:00:00
abstract::Mouse anterior-posterior axis polarization is preceded by formation of the distal visceral endoderm (DVE) by unknown mechanisms. Here, we show by in vitro culturing of embryos immediately after implantation in microfabricated cavities that the external mechanical cues exerted on the embryo are crucial for DVE formatio...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2013.09.026
更新日期:2013-10-28 00:00:00
abstract::Metastasizing breast carcinoma cells have been hypothesized to mobilize tissue-invasive activity by co-opting the proteolytic systems employed by normal mammary epithelial cells undergoing branching morphogenesis. However, the critical effectors underlying morphogenesis remain unidentified, and their relationship to b...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2018.08.025
更新日期:2018-10-22 00:00:00
abstract::Merlin encoded by the Nf2 gene is a bona fide tumor suppressor that has been implicated in regulation of both the Hippo-Yap and Rac1-Pak1 pathways. Using genetically engineered murine liver models, we show that co-deletion of Rac1 with Nf2 blocks tumor initiation but paradoxically exacerbates hepatomegaly induced by N...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2016.09.027
更新日期:2016-11-21 00:00:00
abstract::Actomyosin flows are involved in a variety of cellular processes, including cytokinesis, cell migration, polarization, and morphogenesis. In epithelia, flow polarization orients cell deformations. It is unclear, however, how flows are polarized and how global patterns of junction remodeling emerge from flow polarizati...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2013.06.020
更新日期:2013-07-29 00:00:00
abstract::Polarity is a prominent feature of both chemotaxis and cytokinesis. In chemotaxis, polarity is established by local accumulation of PI(3,4,5)P3 at the cell's leading edge, achieved through temporal and spatial regulation of PI3 kinases and the tumor suppressor, PTEN. We find that as migrating D. discoideum cells round...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2005.02.010
更新日期:2005-04-01 00:00:00
abstract::Morphogenesis of wavy epidermal pavement cells in plants has fascinated researchers for decades. A mechanical mechanism had been proposed in which the anticlinal cell walls, forming the in-plane cell borders, feature contiguous stiff and soft zones that generate waves upon stretching. We replicated this model as desig...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2019.05.001
更新日期:2019-07-01 00:00:00
abstract::Plants assemble beneficial root-associated microbiomes to support growth, especially in nutrient-poor conditions. To do so, however, plants have to suppress their immune system. Reporting in Nature, Castrillo et al. (2017) identified PHOSPHATE STARVATION RESPONSE1 (PHR1) as a central regulator in this balance between ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2017.03.019
更新日期:2017-04-10 00:00:00