Abstract：
:A central enigma in epigenetics is how epigenetic factors are guided to specific genomic sites for their function. Previously, we reported that a Piwi-piRNA complex associates with the piRNA-complementary site in the Drosophila genome and regulates its epigenetic state. Here, we report that Piwi-piRNA complexes bind to numerous piRNA-complementary sequences throughout the genome, implicating piRNAs as a major mechanism that guides Piwi and Piwi-associated epigenetic factors to program the genome. To test this hypothesis, we demonstrate that inserting piRNA-complementary sequences to an ectopic site leads to Piwi, HP1a, and Su(var)3-9 recruitment to the site as well as H3K9me2/3 enrichment and reduced RNA polymerase II association, indicating that piRNA is both necessary and sufficient to recruit Piwi and epigenetic factors to specific genomic sites. Piwi deficiency drastically changed the epigenetic landscape and polymerase II profile throughout the genome, revealing the Piwi-piRNA mechanism as a major epigenetic programming mechanism in Drosophila.
journal_name
Dev Celljournal_title
Developmental cellauthors
Huang XA,Yin H,Sweeney S,Raha D,Snyder M,Lin Hdoi
10.1016/j.devcel.2013.01.023subject
Has Abstractpub_date
2013-03-11 00:00:00pages
502-16issue
5eissn
1534-5807issn
1878-1551pii
S1534-5807(13)00071-3journal_volume
24pub_type
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