Abstract:
:Rab GTPases define the vesicle trafficking pathways underpinning cell polarization and migration. Here, we find that Rab4, Rab11, and Rab14 and the candidate Rab GDP-GTP exchange factors (GEFs) FAM116A and AVL9 are required for cell migration. Rab14 and its GEF FAM116A localize to and act on an intermediate compartment of the transferrin-recycling pathway prior to Rab11 and after Rab5 and Rab4. This Rab14 intermediate recycling compartment has specific functions in migrating cells discrete from early and recycling endosomes. Rab14-depleted cells show increased N-cadherin levels at junctional complexes and cannot resolve cell-cell junctions. This is due to decreased shedding of cell-surface N-cadherin by the ADAM family protease ADAM10/Kuzbanian. In FAM116A- and Rab14-depleted cells, ADAM10 accumulates in a transferrin-positive endocytic compartment, and the cell-surface level of ADAM10 is correspondingly reduced. FAM116 and Rab14 therefore define an endocytic recycling pathway needed for ADAM protease trafficking and regulation of cell-cell junctions.
journal_name
Dev Celljournal_title
Developmental cellauthors
Linford A,Yoshimura S,Nunes Bastos R,Langemeyer L,Gerondopoulos A,Rigden DJ,Barr FAdoi
10.1016/j.devcel.2012.04.010subject
Has Abstractpub_date
2012-05-15 00:00:00pages
952-66issue
5eissn
1534-5807issn
1878-1551pii
S1534-5807(12)00187-6journal_volume
22pub_type
杂志文章abstract::In multicellular organisms, cell size is tightly controlled by nutrients and growth factors. Increasing ambient glucose induces enhanced protein synthesis and cell size. Continued exposure of cells to high glucose in vivo, as apparent under pathological conditions, results in cell hypertrophy and tissue damage. We dem...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2009.05.010
更新日期:2009-07-01 00:00:00
abstract::CPEB is a sequence-specific RNA binding protein that regulates translation during vertebrate oocyte maturation. Adult female CPEB knockout mice contained vestigial ovaries that were devoid of oocytes; ovaries from mid-gestation embryos contained oocytes that were arrested at the pachytene stage. Male CPEB null mice al...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(01)00025-9
更新日期:2001-08-01 00:00:00
abstract::For many years, biologists have focused on the role of Pitx2, expressed on the left side of developing embryos, in governing organ laterality. Here, we identify a different pathway during left-right asymmetry initiated by the right side of the embryo. Surprisingly, this conserved mechanism is orchestrated by the extra...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2018.08.002
更新日期:2018-09-10 00:00:00
abstract::Differentiated cells have evolved mechanisms to adapt the functions of the late secretory pathway to the specific needs of the organism. Reporting in this issue of Developmental Cell, Polishchuk et al. (2014) demonstrate that hepatocytes utilize a unique exocytic aspect of the late endosomal/lysosomal compartment to m...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2014.06.002
更新日期:2014-06-23 00:00:00
abstract::In this issue of Developmental Cell, Schuster et al. (2014) describe the signals regulated by the bHLH transcription factor HEC1 during Arabidopsis stem cell maintenance. HEC1 acts antagonistically with other factors, integrating multiple cues to provide a balance between cellular differentiation and proliferation. ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2014.02.006
更新日期:2014-02-24 00:00:00
abstract::A central enigma in epigenetics is how epigenetic factors are guided to specific genomic sites for their function. Previously, we reported that a Piwi-piRNA complex associates with the piRNA-complementary site in the Drosophila genome and regulates its epigenetic state. Here, we report that Piwi-piRNA complexes bind t...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2013.01.023
更新日期:2013-03-11 00:00:00
abstract::Cell competition promotes the elimination of weaker cells from a growing population. Here we investigate how cells of Drosophila wing imaginal discs distinguish "winners" from "losers" during cell competition. Using genomic and functional assays, we have identified several factors implicated in the process, including ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2010.05.010
更新日期:2010-06-15 00:00:00
abstract::The large GTPase Dynamin 2 (Dyn2) is markedly upregulated in pancreatic cancer, is a potent activator of metastatic migration, and is required for Rac1-mediated formation of lamellipodia. Here we demonstrate an unexpected mechanism of Dyn2 action in these contexts via direct binding to the Rac1 guanine nucleotide exch...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2013.02.010
更新日期:2013-03-25 00:00:00
abstract::In this issue of Developmental Cell, Isabella and Horne-Badovinac (2016) show that Rab10 directs site-specific secretion of basement membrane components, which assemble into fibrils that spool out to elongate the Drosophila egg chamber. These findings establish the basement membrane's active role in tissue sculpting. ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2016.06.028
更新日期:2016-07-11 00:00:00
abstract::Novel body structures are often generated by the redeployment of ancestral components of the genome. In this issue of Developmental Cell, Glassford et al. (2015) present a thorough analysis of the co-option of a gene regulatory network in the origin of an evolutionary novelty. ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2015.08.022
更新日期:2015-09-14 00:00:00
abstract::The cytoplasm is not a homogenous solution but instead consists of large dynamic assemblies that arise from transient molecular interactions. Some of these structures have been shown to represent liquid droplets of concentrated protein and RNA. Liquid phase separation of cytoplasm may be a fundamental principle of cyt...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.06.013
更新日期:2011-07-19 00:00:00
abstract::The adult, virgin mammary gland is a highly organized tree-like structure formed by ducts with hollowed lumen. Although lumen formation during pubertal development appears to involve apoptosis, the molecular mechanisms that regulate this process are not known. Here, we demonstrate that disruption of the BH3-only proap...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2006.12.003
更新日期:2007-02-01 00:00:00
abstract::Local translation allows spatial control of gene expression. Here, we performed a dual protein-mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. 32 mRNAs displayed specific cytoplasmic localizations with local translation at unexpected locations, including cytoplasmic protrusi...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2020.07.010
更新日期:2020-09-28 00:00:00
abstract::Serrate (SE) is a key component in RNA metabolism. Little is known about whether and how it can regulate epigenetic silencing. Here, we report histone methyltransferases ATXR5 and ATXR6 (ATXR5/6) as novel partners of SE. ATXR5/6 deposit histone 3 lysine 27 monomethylation (H3K27me1) to promote heterochromatin formatio...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2018.05.023
更新日期:2018-06-18 00:00:00
abstract::Small repeat-associated siRNAs (rasiRNAs) mediate silencing of retrotransposons and the Stellate locus. Mutations in the Drosophila rasiRNA pathway genes armitage and aubergine disrupt embryonic axis specification, triggering defects in microtubule polarization as well as asymmetric localization of mRNA and protein de...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2006.12.001
更新日期:2007-01-01 00:00:00
abstract::Constitutive exocytosis delivers newly synthesized proteins, lipids, and other molecules from the Golgi apparatus to the cell surface. This process is mediated by vesicles, which bud off the trans-Golgi network, move along cytoskeletal filaments, and fuse with the plasma membrane. Here, we show that the small GTPase R...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2007.06.010
更新日期:2007-08-01 00:00:00
abstract::CLIPs are microtubule plus end-associated proteins that mediate interactions required for cell polarity and cell division. Here we demonstrate that budding yeast Bik1, unlike its human ortholog CLIP-170, is targeted to the microtubule plus end by a kinesin-dependent transport mechanism. Bik1 forms a complex with the k...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2004.05.001
更新日期:2004-06-01 00:00:00
abstract::Numerous signals drive the proliferative expansion of the distal endoderm and the underlying mesenchyme during lung branching morphogenesis, but little is known about how these signals are integrated. Here, we show by analysis of conditional double mutants that the two T-box transcription factor genes Tbx2 and Tbx3 ac...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2016.08.007
更新日期:2016-10-24 00:00:00
abstract::Studies of myoblast fusion in Drosophila have revealed numerous signaling and structural proteins that regulate this critical event in muscle development. A paper by Chen et al. published in the September 19 issue of Cell adds ARF6, a GTPase, and one of its guanine nucleotide exchange factors to this set of players. ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(03)00298-3
更新日期:2003-10-01 00:00:00
abstract::Egg production declines with age in many species, a process linked with stem cell loss. Diet-dependent signaling has emerged as critical for stem cell maintenance during aging. Follicle stem cells (FSCs) in the Drosophila ovary are exquisitely responsive to diet-induced signals including Hedgehog (Hh) and insulin-IGF ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2018.08.008
更新日期:2018-09-24 00:00:00
abstract::Gene expression often requires interaction between promoters and distant enhancers, which occur within the context of highly organized topologically associating domains (TADs). Using a series of engineered chromosomal rearrangements at the Shh locus, we carried out an extensive fine-scale characterization of the facto...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2016.10.015
更新日期:2016-12-05 00:00:00
abstract::Tissue folding is a fundamental process that shapes epithelia into complex 3D organs. The initial positioning of folds is the foundation for the emergence of correct tissue morphology. Mechanisms forming individual folds have been studied, but the precise positioning of folds in complex, multi-folded epithelia is less...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2019.09.009
更新日期:2019-11-04 00:00:00
abstract::Tissues have a natural capacity to replace dying cells and to heal wounds. This ability resides in resident stem cells, which self-renew, preserve, and repair their tissue during homeostasis and following injury. The skin epidermis and its appendages are subjected to daily assaults from the external environment. A hig...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2017.10.001
更新日期:2017-11-20 00:00:00
abstract::Epithelial cells acquire mesenchymal characteristics during development, wound healing and inflammation, and in cancer and fibrosis. With increasing appreciation of different roles of epithelial-mesenchymal transition (EMT), we address the question of how to define and recognize EMT processes and discuss their propert...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2019.04.026
更新日期:2019-05-06 00:00:00
abstract::Many human genes generate mRNAs with alternative 3' untranslated regions (3' UTRs), which can modulate protein function. In a recent Cell paper, Ma and Mayr (2018) unveil a major membraneless organelle, termed the TIS granule-endoplasmic reticulum (TIGER), which enables specific 3' UTRs to encipher protein localizatio...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.11.022
更新日期:2018-12-03 00:00:00
abstract::Centromere chromatin containing histone H3 variant CENP-A is required for accurate chromosome segregation as a foundation for kinetochore assembly. Human centromere chromatin assembles on a part of the long α-satellite (alphoid) DNA array, where it is flanked by pericentric heterochromatin. Heterochromatin spreads int...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2016.05.006
更新日期:2016-06-06 00:00:00
abstract::Lysosomes degrade macromolecular cargos, recycle catabolites, and serve as signaling platforms to maintain cell homeostasis, but their role at the tissue level is unclear. Here, we investigate lysosome regulation and function during C. elegans molting, a specialized extracellular matrix (ECM) remodeling process essent...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2019.10.020
更新日期:2020-01-06 00:00:00
abstract::A balance between Six2-dependent self-renewal and canonical Wnt signaling-directed commitment regulates mammalian nephrogenesis. Intersectional studies using chromatin immunoprecipitation and transcriptional profiling identified direct target genes shared by each pathway within nephron progenitors. Wnt4 and Fgf8 are e...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2012.07.008
更新日期:2012-09-11 00:00:00
abstract::Lymphatic vessels are essential for tissue homeostasis and immune surveillance and contribute to pathological conditions. Lymphatic endothelium differentiates from veins and forms an independent vascular tree with only few connections to the venous circulation. Failure of blood and lymphatic vessel separation results ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2010.01.009
更新日期:2010-03-16 00:00:00
abstract::It is intuitive to speculate that nutrient availability may influence differentiation of mammalian cells. Nonetheless, a comprehensive complement of the molecular determinants involved in this process has not been elucidated yet. Here, we have investigated how nutrients (glucose) affect skeletal myogenesis. Glucose re...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2008.02.004
更新日期:2008-05-01 00:00:00