Abstract:
:Exposing cells to folding stress causes a subset of their proteins to misfold and accumulate in inclusion bodies (IBs). IB formation and clearance are both active processes, but little is known about their mechanism. To shed light on this issue, we performed a screen with over 4,000 fluorescently tagged yeast proteins for co-localization with a model misfolded protein that marks IBs during folding stress. We identified 13 proteins that co-localize to IBs. Remarkably, one of these IB proteins, the uncharacterized and conserved protein Iml2, exhibited strong physical interactions with lipid droplet (LD) proteins. Indeed, we here show that IBs and LDs are spatially and functionally linked. We further demonstrate a mechanism for IB clearance via a sterol-based metabolite emanating from LDs. Our findings therefore uncover a function for Iml2 and LDs in regulating a critical stage of cellular proteostasis.
journal_name
Dev Celljournal_title
Developmental cellauthors
Moldavski O,Amen T,Levin-Zaidman S,Eisenstein M,Rogachev I,Brandis A,Kaganovich D,Schuldiner Mdoi
10.1016/j.devcel.2015.04.015subject
Has Abstractpub_date
2015-06-08 00:00:00pages
603-10issue
5eissn
1534-5807issn
1878-1551pii
S1534-5807(15)00279-8journal_volume
33pub_type
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