Abstract：
:Centromeres are essential for accurate chromosome segregation and are marked by centromere protein A (CENP-A) nucleosomes. Mis-targeted CENP-A chromatin has been shown to seed centromeres at non-centromeric DNA. However, the requirements for such de novo centromere formation and transmission in vivo remain unknown. Here, we employ Drosophila melanogaster and the LacI/lacO system to investigate the ability of targeted de novo centromeres to assemble and be inherited through development. De novo centromeres form efficiently at six distinct genomic locations, which include actively transcribed chromatin and heterochromatin, and cause widespread chromosomal instability. During tethering, de novo centromeres sometimes prevail, causing the loss of the endogenous centromere via DNA breaks and HP1-dependent epigenetic inactivation. Transient induction of de novo centromeres and chromosome healing in early embryogenesis show that, once established, these centromeres can be maintained through development. Our results underpin the ability of CENP-A chromatin to establish and sustain mitotic centromere function in Drosophila.
journal_name
Dev Celljournal_title
Developmental cellauthors
Palladino J,Chavan A,Sposato A,Mason TD,Mellone BGdoi
10.1016/j.devcel.2020.01.005subject
Has Abstractpub_date
2020-02-10 00:00:00pages
379-394.e7issue
3eissn
1534-5807issn
1878-1551pii
S1534-5807(20)30006-Xjournal_volume
52pub_type
杂志文章abstract：:Microtubules are non-covalent mesoscale polymers central to the eukaryotic cytoskeleton. Microtubule structure, dynamics, and mechanics are modulated by a cell's choice of tubulin isoforms and post-translational modifications, a "tubulin code," which is thought to support the diverse morphology and dynamics of microtu...
journal_title：Developmental cell
pub_type： 杂志文章,评审
doi：10.1016/j.devcel.2020.06.008
更新日期：2020-07-06 00:00:00
abstract：:We developed a comprehensive dataset that samples the mouse transcriptome every 6 hr, from gastrulation through organogenesis. We observe an abrupt increase in overall transcript diversity at the onset of organogenesis (e8.0); the genes that comprise these changes are preferentially clustered along chromosome 7 and co...
journal_title：Developmental cell
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abstract：:Cells that undergo epithelial-to-mesenchymal transitions (EMTs) commonly switch from expressing E-cadherin to N-cadherin. But why this occurs is not well understood. In the current issue of Developmental Cell, Scarpa et al. (2015) identify a reason: cadherin switching controls Rac signaling to determine how cell locom...
journal_title：Developmental cell
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journal_title：Developmental cell
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2012.05.014
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journal_title：Developmental cell
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journal_title：Developmental cell
pub_type： 杂志文章,评审
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journal_title：Developmental cell
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doi：10.1016/j.devcel.2020.01.025
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2011.06.013
更新日期：2011-07-19 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/s1534-5807(01)00047-8
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2012.12.015
更新日期：2013-02-11 00:00:00
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journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2017.03.019
更新日期：2017-04-10 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2016.05.002
更新日期：2016-06-06 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2014.08.027
更新日期：2014-10-27 00:00:00
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journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2013.02.005
更新日期：2013-02-25 00:00:00
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journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2010.01.002
更新日期：2010-01-19 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2010.05.010
更新日期：2010-06-15 00:00:00
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journal_title：Developmental cell
pub_type： 评论,杂志文章
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journal_title：Developmental cell
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journal_title：Developmental cell
pub_type： 评论,杂志文章
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pub_type： 传,社论,历史文章
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更新日期：2007-12-01 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
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journal_title：Developmental cell
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journal_title：Developmental cell
pub_type： 评论,杂志文章
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更新日期：2016-10-10 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章,评审
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更新日期：2002-03-01 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2020.02.012
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journal_title：Developmental cell
pub_type： 杂志文章
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更新日期：2003-10-01 00:00:00
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journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2018.08.025
更新日期：2018-10-22 00:00:00
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journal_title：Developmental cell
pub_type： 评论,杂志文章
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