Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A Chromatin.


:Centromeres are essential for accurate chromosome segregation and are marked by centromere protein A (CENP-A) nucleosomes. Mis-targeted CENP-A chromatin has been shown to seed centromeres at non-centromeric DNA. However, the requirements for such de novo centromere formation and transmission in vivo remain unknown. Here, we employ Drosophila melanogaster and the LacI/lacO system to investigate the ability of targeted de novo centromeres to assemble and be inherited through development. De novo centromeres form efficiently at six distinct genomic locations, which include actively transcribed chromatin and heterochromatin, and cause widespread chromosomal instability. During tethering, de novo centromeres sometimes prevail, causing the loss of the endogenous centromere via DNA breaks and HP1-dependent epigenetic inactivation. Transient induction of de novo centromeres and chromosome healing in early embryogenesis show that, once established, these centromeres can be maintained through development. Our results underpin the ability of CENP-A chromatin to establish and sustain mitotic centromere function in Drosophila.


Dev Cell


Developmental cell


Palladino J,Chavan A,Sposato A,Mason TD,Mellone BG




Has Abstract


2020-02-10 00:00:00














  • Wnt3a plays a major role in the segmentation clock controlling somitogenesis.

    abstract::The vertebral column derives from somites generated by segmentation of presomitic mesoderm (PSM). Somitogenesis involves a molecular oscillator, the segmentation clock, controlling periodic Notch signaling in the PSM. Here, we establish a novel link between Wnt/beta-catenin signaling and the segmentation clock. Axin2,...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Aulehla A,Wehrle C,Brand-Saberi B,Kemler R,Gossler A,Kanzler B,Herrmann BG

    更新日期:2003-03-01 00:00:00

  • Aging Suppresses Sphingosine-1-Phosphate Chaperone ApoM in Circulation Resulting in Maladaptive Organ Repair.

    abstract::Here, we show that the liver-derived apolipoprotein M (ApoM) protects the lung and kidney from pro-fibrotic insults and that this circulating factor is attenuated in aged mice. Aged mouse hepatocytes exhibit transcriptional suppression of ApoM. This leads to reduced sphingosine-1-phosphate (S1P) signaling via the S1P ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Ding BS,Yang D,Swendeman SL,Christoffersen C,Nielsen LB,Friedman SL,Powell CA,Hla T,Cao Z

    更新日期:2020-06-22 00:00:00

  • Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity.

    abstract::The NAD+-dependent deacetylase Sirtuin 1 (SIRT1) is down-regulated in triple-negative breast cancer. To determine the mechanistic basis by which reduced SIRT1 expression influences processes related to certain aggressive cancers, we examined the consequences of depleting breast cancer cells of SIRT1. We discovered tha...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Latifkar A,Ling L,Hingorani A,Johansen E,Clement A,Zhang X,Hartman J,Fischbach C,Lin H,Cerione RA,Antonyak MA

    更新日期:2019-05-06 00:00:00

  • Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding.

    abstract::Morphological constancy is universal in developing systems. It is unclear whether precise morphogenesis stems from faithful mechanical interpretation of gene expression patterns. We investigate the formation of the cephalic furrow, an epithelial fold that is precisely positioned with a linear morphology. Fold initiati...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Eritano AS,Bromley CL,Bolea Albero A,Schütz L,Wen FL,Takeda M,Fukaya T,Sami MM,Shibata T,Lemke S,Wang YC

    更新日期:2020-04-20 00:00:00

  • Probing intrinsic properties of a robust morphogen gradient in Drosophila.

    abstract::A remarkable feature of development is its reproducibility, the ability to correct embryo-to-embryo variations and instruct precise patterning. In Drosophila, embryonic patterning along the anterior-posterior axis is controlled by the morphogen gradient Bicoid (Bcd). In this article, we describe quantitative studies o...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: He F,Wen Y,Deng J,Lin X,Lu LJ,Jiao R,Ma J

    更新日期:2008-10-01 00:00:00

  • Live free or die: cell-cell adhesion regulates sensitivity to trail-induced apoptosis.

    abstract::The ability of the death ligand TRAIL to induce tumor cell apoptosis has led to the development of TRAIL-based cancer therapies. Reporting recently in Molecular Cell, Lu et al. (2014) show that the basis for differential TRAIL responses involves clustering of death receptor complexes by E-cadherin and the actin cytosk...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Gallegos LL,Brugge JS

    更新日期:2014-07-14 00:00:00

  • Actin Polymerization and ESCRT Trigger Recruitment of the Fusogens Syntaxin-2 and EFF-1 to Promote Membrane Repair in C. elegans.

    abstract::Membrane repair is essential for cell and organism survival. Exocytosis and endocytosis facilitate membrane repair in small wounds within a single cell; however, it remains unclear how large wounds in the plasma membrane are repaired in metazoans. Here, we show that wounding triggers rapid transcriptional upregulation...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Meng X,Yang Q,Yu X,Zhou J,Ren X,Zhou Y,Xu S

    更新日期:2020-09-14 00:00:00

  • Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.

    abstract::How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on th...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

    更新日期:2017-02-27 00:00:00

  • Drosophila neuralized is a ubiquitin ligase that promotes the internalization and degradation of delta.

    abstract::The Drosophila gene neuralized (neur) has long been recognized to be essential for the proper execution of a wide variety of processes mediated by the Notch (N) pathway, but its role in the pathway has been elusive. In this report, we present genetic and biochemical evidence that Neur is a RING-type, E3 ubiquitin liga...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Lai EC,Deblandre GA,Kintner C,Rubin GM

    更新日期:2001-12-01 00:00:00

  • Formation of the BMP activity gradient in the Drosophila embryo.

    abstract::The dorsoventral axis of the Drosophila embryo is patterned by a gradient of bone morphogenetic protein (BMP) ligands. In a process requiring at least three additional extracellular proteins, a broad domain of weak signaling forms and then abruptly sharpens into a narrow dorsal midline peak. Using experimental and com...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Mizutani CM,Nie Q,Wan FY,Zhang YT,Vilmos P,Sousa-Neves R,Bier E,Marsh JL,Lander AD

    更新日期:2005-06-01 00:00:00

  • Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS.

    abstract::Regulating cell proliferation and differentiation in CNS development requires both extraordinary complexity and precision. Neural progenitors receive graded overlapping signals from midline signaling centers, yet each makes a unique cell fate decision in a spatiotemporally restricted pattern. The Nde1-Lis1 complex reg...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Lanctot AA,Peng CY,Pawlisz AS,Joksimovic M,Feng Y

    更新日期:2013-05-13 00:00:00

  • Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells.

    abstract::The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result of subcellular variations in expansion rate that induce local ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Majda M,Grones P,Sintorn IM,Vain T,Milani P,Krupinski P,Zagórska-Marek B,Viotti C,Jönsson H,Mellerowicz EJ,Hamant O,Robert S

    更新日期:2017-11-06 00:00:00

  • An ECM-to-Nucleus Signaling Pathway Activates Lysosomes for C. elegans Larval Development.

    abstract::Lysosomes degrade macromolecular cargos, recycle catabolites, and serve as signaling platforms to maintain cell homeostasis, but their role at the tissue level is unclear. Here, we investigate lysosome regulation and function during C. elegans molting, a specialized extracellular matrix (ECM) remodeling process essent...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Miao R,Li M,Zhang Q,Yang C,Wang X

    更新日期:2020-01-06 00:00:00

  • Flower forms an extracellular code that reveals the fitness of a cell to its neighbors in Drosophila.

    abstract::Cell competition promotes the elimination of weaker cells from a growing population. Here we investigate how cells of Drosophila wing imaginal discs distinguish "winners" from "losers" during cell competition. Using genomic and functional assays, we have identified several factors implicated in the process, including ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Rhiner C,López-Gay JM,Soldini D,Casas-Tinto S,Martín FA,Lombardía L,Moreno E

    更新日期:2010-06-15 00:00:00

  • Recruitment of ectodermal attachment cells via an EGFR-dependent mechanism during the organogenesis of Drosophila proprioceptors.

    abstract::Drosophila proprioceptors (chordotonal organs) are structured as a linear array of four lineage-related cells: a neuron, a glial cell, and two accessory cells, called cap and ligament, between which the neuron is stretched. To function properly as stretch receptors, chordotonal organs must be stably anchored at both e...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Inbal A,Volk T,Salzberg A

    更新日期:2004-08-01 00:00:00

  • Fibronectin regulates epithelial organization during myocardial migration in zebrafish.

    abstract::Several genes have been implicated in heart tube formation, yet we know little about underlying cellular mechanisms. We analyzed the cellular architecture of the migrating myocardial precursors, and find that they form coherent epithelia that mature as they move medially. Mutant analyses indicate that the cardia bifid...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Trinh LA,Stainier DY

    更新日期:2004-03-01 00:00:00

  • Canonical Wnt Signaling Sets the Pace.

    abstract::In this issue of Developmental Cell, Ren et al. (2019) identify the embryonic origin of cardiac pacemaker cells in zebrafish and implicate Wnt5b in promoting their differentiation. Furthermore, canonical Wnt activation in human stem cell-derived cardiac progenitors produces functional pacemaker cells in vitro, advanci...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Burns CG,Burns CE

    更新日期:2019-09-23 00:00:00

  • Structural architecture and functional evolution of Wnts.

    abstract::The crystal structure of a Wnt morphogen bound to its Frizzled receptor ectodomain provides insights into the evolutionary provenance of this complex fold and offers an explanation for why Wnts utilize both lipid- and protein-mediated contacts to engage Frizzleds. ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Bazan JF,Janda CY,Garcia KC

    更新日期:2012-08-14 00:00:00

  • Sensory-neuron subtype-specific transcriptional programs controlling dendrite morphogenesis: genome-wide analysis of Abrupt and Knot/Collier.

    abstract::The transcription factors Abrupt (Ab) and Knot (Kn) act as selectors of distinct dendritic arbor morphologies in two classes of Drosophila sensory neurons, termed class I and class IV, respectively. We performed binding-site mapping and transcriptional profiling of these isolated neurons. Their profiles were similarly...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Hattori Y,Usui T,Satoh D,Moriyama S,Shimono K,Itoh T,Shirahige K,Uemura T

    更新日期:2013-12-09 00:00:00

  • Myoblasts fuse when loner meets ARF6.

    abstract::Studies of myoblast fusion in Drosophila have revealed numerous signaling and structural proteins that regulate this critical event in muscle development. A paper by Chen et al. published in the September 19 issue of Cell adds ARF6, a GTPase, and one of its guanine nucleotide exchange factors to this set of players. ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Donaldson JG

    更新日期:2003-10-01 00:00:00

  • The bacterial cytoskeleton: in vivo dynamics of the actin-like protein Mbl of Bacillus subtilis.

    abstract::Mbl is a bacterial actin homolog that controls cell morphogenesis in Bacillus subtilis. A functional GFP-Mbl fusion protein was used to examine the behavior of the helical cables formed by Mbl protein in live B. subtilis cells. The cables undergo dynamic changes during cell cycle progression. They are stable but not r...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Carballido-López R,Errington J

    更新日期:2003-01-01 00:00:00

  • Neutrophil Extracellular Traps: The Biology of Chromatin Externalization.

    abstract::Neutrophils are essential to the homeostatic mission of safeguarding host tissues, responding rapidly and diversely to breaches of the host's barriers to infection, and returning tissues to a sterile state. In response to specific stimuli, neutrophils extrude modified chromatin structures decorated with specific cytop...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审


    authors: Sollberger G,Tilley DO,Zychlinsky A

    更新日期:2018-03-12 00:00:00

  • Lipoprotein-heparan sulfate interactions in the Hh pathway.

    abstract::The Drosophila lipoprotein particle, Lipophorin, bears lipid-linked morphogens on its surface and is required for long-range signaling activity of Wingless and Hedgehog. Heparan sulfate proteoglycans are also critical for trafficking and signaling of these morphogens. Here we show that Lipophorin interacts with the he...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Eugster C,Panáková D,Mahmoud A,Eaton S

    更新日期:2007-07-01 00:00:00

  • Role of lipid metabolism in smoothened derepression in hedgehog signaling.

    abstract::The binding of Hedgehog (Hh) to its receptor Patched causes derepression of Smoothened (Smo), resulting in the activation of the Hh pathway. Here, we show that Smo activation is dependent on the levels of the phospholipid phosphatidylinositol-4 phosphate (PI4P). Loss of STT4 kinase, which is required for the generatio...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Yavari A,Nagaraj R,Owusu-Ansah E,Folick A,Ngo K,Hillman T,Call G,Rohatgi R,Scott MP,Banerjee U

    更新日期:2010-07-20 00:00:00

  • Directional cell migration, but not proliferation, drives hair placode morphogenesis.

    abstract::Epithelial reorganization involves coordinated changes in cell shapes and movements. This restructuring occurs during formation of placodes, ectodermal thickenings that initiate the morphogenesis of epithelial organs including hair, mammary gland, and tooth. Signaling pathways in ectodermal placode formation are well ...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Ahtiainen L,Lefebvre S,Lindfors PH,Renvoisé E,Shirokova V,Vartiainen MK,Thesleff I,Mikkola ML

    更新日期:2014-03-10 00:00:00

  • Apoptosis and Compensatory Proliferation Signaling Are Coupled by CrkI-Containing Microvesicles.

    abstract::Apoptosis has been implicated in compensatory proliferation signaling (CPS), whereby dying cells induce proliferation in neighboring cells as a means to restore homeostasis. The nature of signaling between apoptotic cells and their neighboring cells remains largely unknown. Here we show that a fraction of apoptotic ce...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Gupta KH,Goldufsky JW,Wood SJ,Tardi NJ,Moorthy GS,Gilbert DZ,Zayas JP,Hahm E,Altintas MM,Reiser J,Shafikhani SH

    更新日期:2017-06-19 00:00:00

  • Chemokine signaling directs trunk lymphatic network formation along the preexisting blood vasculature.

    abstract::The lymphatic system is crucial for fluid homeostasis, immune responses, and numerous pathological processes. However, the molecular mechanisms responsible for establishing the anatomical form of the lymphatic vascular network remain largely unknown. Here, we show that chemokine signaling provides critical guidance cu...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Cha YR,Fujita M,Butler M,Isogai S,Kochhan E,Siekmann AF,Weinstein BM

    更新日期:2012-04-17 00:00:00

  • Stopped in translation: EMT control meets eukaryotic elongation.

    abstract::Recently reporting in Molecular Cell, Hussey et al. (2011) find that hnRNP E1 inhibits the translation of several genes essential for the epithelial-to-mesenchymal transition (EMT) by blocking eEF1A1 release during translation elongation. Phosphorylation of hnRNP E1 in response to TGF-β signaling disrupts the hnRNP E1...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章


    authors: Zhang YE

    更新日期:2011-03-15 00:00:00

  • Architecture and Characteristics of Bacterial Nanotubes.

    abstract::Bacteria display an array of contact-dependent interaction systems that have evolved to facilitate direct cell-to-cell communication. We have previously identified a mode of bacterial communication mediated by nanotubes bridging neighboring cells. Here, we elucidate nanotube architecture, dynamics, and molecular compo...

    journal_title:Developmental cell

    pub_type: 杂志文章


    authors: Dubey GP,Malli Mohan GB,Dubrovsky A,Amen T,Tsipshtein S,Rouvinski A,Rosenberg A,Kaganovich D,Sherman E,Medalia O,Ben-Yehuda S

    更新日期:2016-02-22 00:00:00

  • Extracellular Vesicles: Satellites of Information Transfer in Cancer and Stem Cell Biology.

    abstract::The generation and shedding of extracellular vesicles (EVs), including exosomes and microvesicles (MVs), by cells has emerged as a form of intercellular communication with important roles in several physiological processes and diseases such as cancer. These membrane-enclosed packets can transfer specific proteins, RNA...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审


    authors: Desrochers LM,Antonyak MA,Cerione RA

    更新日期:2016-05-23 00:00:00