Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A Chromatin.

Abstract:

:Centromeres are essential for accurate chromosome segregation and are marked by centromere protein A (CENP-A) nucleosomes. Mis-targeted CENP-A chromatin has been shown to seed centromeres at non-centromeric DNA. However, the requirements for such de novo centromere formation and transmission in vivo remain unknown. Here, we employ Drosophila melanogaster and the LacI/lacO system to investigate the ability of targeted de novo centromeres to assemble and be inherited through development. De novo centromeres form efficiently at six distinct genomic locations, which include actively transcribed chromatin and heterochromatin, and cause widespread chromosomal instability. During tethering, de novo centromeres sometimes prevail, causing the loss of the endogenous centromere via DNA breaks and HP1-dependent epigenetic inactivation. Transient induction of de novo centromeres and chromosome healing in early embryogenesis show that, once established, these centromeres can be maintained through development. Our results underpin the ability of CENP-A chromatin to establish and sustain mitotic centromere function in Drosophila.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Palladino J,Chavan A,Sposato A,Mason TD,Mellone BG

doi

10.1016/j.devcel.2020.01.005

subject

Has Abstract

pub_date

2020-02-10 00:00:00

pages

379-394.e7

issue

3

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(20)30006-X

journal_volume

52

pub_type

杂志文章
  • Geometrical Details Matter for Mechanical Modeling of Cell Morphogenesis.

    abstract::Morphogenesis of wavy epidermal pavement cells in plants has fascinated researchers for decades. A mechanical mechanism had been proposed in which the anticlinal cell walls, forming the in-plane cell borders, feature contiguous stiff and soft zones that generate waves upon stretching. We replicated this model as desig...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2019.05.001

    authors: Bidhendi AJ,Geitmann A

    更新日期:2019-07-01 00:00:00

  • SLIT/ROBO1 signaling suppresses mammary branching morphogenesis by limiting basal cell number.

    abstract::In the field of breast biology, there is a growing appreciation for the "gatekeeping function" of basal cells during development and disease processes yet mechanisms regulating the generation of these cells are poorly understood. Here, we report that the proliferation of basal cells is controlled by SLIT/ROBO1 signali...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2011.05.012

    authors: Macias H,Moran A,Samara Y,Moreno M,Compton JE,Harburg G,Strickland P,Hinck L

    更新日期:2011-06-14 00:00:00

  • HIF1α Represses Cell Stress Pathways to Allow Proliferation of Hypoxic Fetal Cardiomyocytes.

    abstract::Transcriptional mediators of cell stress pathways, including HIF1α, ATF4, and p53, are key to normal development and play critical roles in disease, including ischemia and cancer. Despite their importance, mechanisms by which pathways mediated by these transcription factors interact with one another are not fully unde...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2015.04.021

    authors: Guimarães-Camboa N,Stowe J,Aneas I,Sakabe N,Cattaneo P,Henderson L,Kilberg MS,Johnson RS,Chen J,McCulloch AD,Nobrega MA,Evans SM,Zambon AC

    更新日期:2015-06-08 00:00:00

  • MT1-MMP-dependent control of skeletal stem cell commitment via a β1-integrin/YAP/TAZ signaling axis.

    abstract::In vitro, topographical and biophysical cues arising from the extracellular matrix (ECM) direct skeletal stem cell (SSC) commitment and differentiation. However, the mechanisms by which the SSC-ECM interface is regulated and the outcome of such interactions on stem cell fate in vivo remain unknown. Here we demonstrate...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2013.04.011

    authors: Tang Y,Rowe RG,Botvinick EL,Kurup A,Putnam AJ,Seiki M,Weaver VM,Keller ET,Goldstein S,Dai J,Begun D,Saunders T,Weiss SJ

    更新日期:2013-05-28 00:00:00

  • A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting.

    abstract::Local translation allows spatial control of gene expression. Here, we performed a dual protein-mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. 32 mRNAs displayed specific cytoplasmic localizations with local translation at unexpected locations, including cytoplasmic protrusi...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2020.07.010

    authors: Chouaib R,Safieddine A,Pichon X,Imbert A,Kwon OS,Samacoits A,Traboulsi AM,Robert MC,Tsanov N,Coleno E,Poser I,Zimmer C,Hyman A,Le Hir H,Zibara K,Peter M,Mueller F,Walter T,Bertrand E

    更新日期:2020-09-28 00:00:00

  • DNA Damage Promotes Epithelial Hyperplasia and Fate Mis-specification via Fibroblast Inflammasome Activation.

    abstract::DNA crosslinking agents are commonly used in cancer chemotherapy; however, responses of normal tissues to these agents have not been widely investigated. We reveal in mouse interfollicular epidermal, mammary and hair follicle epithelia that genotoxicity does not promote apoptosis but paradoxically induces hyperplasia ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2020.09.021

    authors: Seldin L,Macara IG

    更新日期:2020-12-07 00:00:00

  • Directional cell migration, but not proliferation, drives hair placode morphogenesis.

    abstract::Epithelial reorganization involves coordinated changes in cell shapes and movements. This restructuring occurs during formation of placodes, ectodermal thickenings that initiate the morphogenesis of epithelial organs including hair, mammary gland, and tooth. Signaling pathways in ectodermal placode formation are well ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2014.02.003

    authors: Ahtiainen L,Lefebvre S,Lindfors PH,Renvoisé E,Shirokova V,Vartiainen MK,Thesleff I,Mikkola ML

    更新日期:2014-03-10 00:00:00

  • The cytokinin-activated transcription factor ARR2 promotes plant immunity via TGA3/NPR1-dependent salicylic acid signaling in Arabidopsis.

    abstract::Cytokinins affect plant immunity to various pathogens; however, the mechanisms coupling plant-derived cytokinins to pathogen responses have been elusive. Here, we found that plant-derived cytokinins promote resistance of Arabidopsis to Pseudomonas syringae pv. tomato DC3000 (Pst). Modulated cytokinin levels or signali...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2010.07.011

    authors: Choi J,Huh SU,Kojima M,Sakakibara H,Paek KH,Hwang I

    更新日期:2010-08-17 00:00:00

  • EGF receptor signaling regulates pulses of cell delamination from the Drosophila ectoderm.

    abstract::Many different intercellular signaling pathways are known but, for most, it is unclear whether they can generate oscillating cell behaviors. Here we use time-lapse analysis of Drosophila embryogenesis to show that oenocytes delaminate from the ectoderm in discrete bursts of three. This pulsatile process has a 1 hour p...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2004.10.016

    authors: Brodu V,Elstob PR,Gould AP

    更新日期:2004-12-01 00:00:00

  • HEC of a job regulating stem cells.

    abstract::In this issue of Developmental Cell, Schuster et al. (2014) describe the signals regulated by the bHLH transcription factor HEC1 during Arabidopsis stem cell maintenance. HEC1 acts antagonistically with other factors, integrating multiple cues to provide a balance between cellular differentiation and proliferation. ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2014.02.006

    authors: Sparks EE,Benfey PN

    更新日期:2014-02-24 00:00:00

  • Epithelial Morphogenesis: Size Matters.

    abstract::In this issue of Developmental Cell, Gibson and colleagues (Ramanathan et al., 2019) investigate the relationship between size and shape in epithelial cells. They find that cell size impacts cell shape, with unexpected consequences for epithelial growth and morphogenesis, particularly the clonal dynamics of epithelial...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2019.09.015

    authors: Thompson BJ

    更新日期:2019-10-07 00:00:00

  • The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation.

    abstract::Centrosomes are important cell organizers. They consist of a pair of centrioles surrounded by pericentriolar material (PCM) that expands dramatically during mitosis-a process termed centrosome maturation. How centrosomes mature remains mysterious. Here, we identify a domain in Drosophila Cnn that appears to be phospho...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2014.02.013

    authors: Conduit PT,Feng Z,Richens JH,Baumbach J,Wainman A,Bakshi SD,Dobbelaere J,Johnson S,Lea SM,Raff JW

    更新日期:2014-03-31 00:00:00

  • Maintaining muscle mitochondria via transsynaptic signaling.

    abstract::Dominant VAPB mutations are implicated in neurodegenerative disease, including amyotrophic lateral sclerosis and spinal muscular atrophy. In the current issue, Han et al. (2012) uncover a mechanism through which the secreted VAPB MSP domain regulates actin organization and mitochondrial function in muscle cells throug...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2012.01.021

    authors: Long JB,Van Vactor D

    更新日期:2012-02-14 00:00:00

  • The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments.

    abstract::To ensure equal chromosome segregation during mitosis, the macromolecular kinetochore must remain attached to depolymerizing microtubules, which drive chromosome movements. How kinetochores associate with depolymerizing microtubules, which undergo dramatic structural changes forming curved protofilaments, has yet to b...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2012.09.012

    authors: Schmidt JC,Arthanari H,Boeszoermenyi A,Dashkevich NM,Wilson-Kubalek EM,Monnier N,Markus M,Oberer M,Milligan RA,Bathe M,Wagner G,Grishchuk EL,Cheeseman IM

    更新日期:2012-11-13 00:00:00

  • Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding.

    abstract::Morphological constancy is universal in developing systems. It is unclear whether precise morphogenesis stems from faithful mechanical interpretation of gene expression patterns. We investigate the formation of the cephalic furrow, an epithelial fold that is precisely positioned with a linear morphology. Fold initiati...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2020.02.012

    authors: Eritano AS,Bromley CL,Bolea Albero A,Schütz L,Wen FL,Takeda M,Fukaya T,Sami MM,Shibata T,Lemke S,Wang YC

    更新日期:2020-04-20 00:00:00

  • External mechanical cues trigger the establishment of the anterior-posterior axis in early mouse embryos.

    abstract::Mouse anterior-posterior axis polarization is preceded by formation of the distal visceral endoderm (DVE) by unknown mechanisms. Here, we show by in vitro culturing of embryos immediately after implantation in microfabricated cavities that the external mechanical cues exerted on the embryo are crucial for DVE formatio...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2013.09.026

    authors: Hiramatsu R,Matsuoka T,Kimura-Yoshida C,Han SW,Mochida K,Adachi T,Takayama S,Matsuo I

    更新日期:2013-10-28 00:00:00

  • C. elegans epidermal wounding induces a mitochondrial ROS burst that promotes wound repair.

    abstract::Reactive oxygen species (ROS) such as hydrogen peroxide are generated at wound sites and act as long-range signals in wound healing. The roles of other ROS in wound repair are little explored. Here, we reveal a cytoprotective role for mitochondrial ROS (mtROS) in Caenorhabditis elegans skin wound healing. We show that...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2014.08.002

    authors: Xu S,Chisholm AD

    更新日期:2014-10-13 00:00:00

  • Out of Phase: How IPMK Inhibits TFEB.

    abstract::The expression of autophagy and lysosomal genes is coordinated by the transcription factor EB (TFEB). In this issue of Developmental Cell, Chen et al. identify an evolutionary conserved mode of TFEB regulation, which entails the inhibition of TFEB phase separation in the nucleus by inositol polyphosphate multikinase. ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2020.11.005

    authors: Ferrari L,Martens S

    更新日期:2020-12-07 00:00:00

  • Plant development makes strides in Vermont.

    abstract::Plants are an excellent system for studying developmental biology. Depending on the species, they can be easily transformed and mutagenized, undergo grafts, tolerate changes in chromosome number, and provide fertile offspring after wide species crosses. Most importantly, they are perpetually embryonic, and thus develo...

    journal_title:Developmental cell

    pub_type:

    doi:10.1016/s1534-5807(02)00299-x

    authors: Fletcher JC,Hake S

    更新日期:2002-10-01 00:00:00

  • Dissecting mitochondrial fusion.

    abstract::Because mitochondria have outer and inner membranes, the fusion of two mitochondria requires the coordinated fusion of four lipid bilayers. Fusion of the outer membranes requires mitofusins. A new study published in Cell (Meeusen et al., 2006) shows that inner membrane fusion requires the dynamin-related protein Mgm1/...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2006.10.009

    authors: Chan DC

    更新日期:2006-11-01 00:00:00

  • A zyxin-mediated mechanism for actin stress fiber maintenance and repair.

    abstract::To maintain mechanical homeostasis, cells must recognize and respond to changes in cytoskeletal integrity. By imaging live cells expressing fluorescently tagged cytoskeletal proteins, we observed that actin stress fibers undergo local, acute, force-induced elongation and thinning events that compromise their stress tr...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2010.08.008

    authors: Smith MA,Blankman E,Gardel ML,Luettjohann L,Waterman CM,Beckerle MC

    更新日期:2010-09-14 00:00:00

  • Neuronal osmotransduction: push-activating TRPV1 with microtubules.

    abstract::Regulating the osmotic pressure of our body fluids relies on osmosensory neurons that depolarize when their volume decreases. Recently in Neuron, Prager-Khoutorsky et al. (2014) report that this depolarization arises from direct interactions between the transient receptor potential channel TRPV1 and microtubules, whic...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2014.08.012

    authors: Andrés M,Göpfert MC

    更新日期:2014-08-25 00:00:00

  • Intrinsic tumor suppression and epithelial maintenance by endocytic activation of Eiger/TNF signaling in Drosophila.

    abstract::Oncogenic alterations in epithelial tissues often trigger apoptosis, suggesting an evolutionary mechanism by which organisms eliminate aberrant cells from epithelia. In Drosophila imaginal epithelia, clones of cells mutant for tumor suppressors, such as scrib or dlg, lose their polarity and are eliminated by cell deat...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2009.01.002

    authors: Igaki T,Pastor-Pareja JC,Aonuma H,Miura M,Xu T

    更新日期:2009-03-01 00:00:00

  • The CKI1 Histidine Kinase Specifies the Female Gametic Precursor of the Endosperm.

    abstract::Since the discovery of double fertilization, it has been recognized that flowering plants produce two highly dimorphic female gametes, the egg cell and central cell. These give rise, respectively, to the embryo and the endosperm, a nourishing tissue unique to flowering plants. Here we show that in Arabidopsis, endospe...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2016.03.009

    authors: Yuan L,Liu Z,Song X,Johnson C,Yu X,Sundaresan V

    更新日期:2016-04-04 00:00:00

  • Foxf2 Is Required for Brain Pericyte Differentiation and Development and Maintenance of the Blood-Brain Barrier.

    abstract::Pericytes are critical for cerebrovascular maturation and development of the blood-brain barrier (BBB), but their role in maintenance of the adult BBB, and how CNS pericytes differ from those of other tissues, is less well understood. We show that the forkhead transcription factor Foxf2 is specifically expressed in pe...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2015.05.008

    authors: Reyahi A,Nik AM,Ghiami M,Gritli-Linde A,Pontén F,Johansson BR,Carlsson P

    更新日期:2015-07-06 00:00:00

  • The cell adhesion molecule echinoid functions as a tumor suppressor and upstream regulator of the Hippo signaling pathway.

    abstract::The Hippo (Hpo) signaling pathway controls tissue growth and organ size in species ranging from Drosophila to mammals and is deregulated in a wide range of human cancers. The core pathway consists of the Hpo/Warts (Wts) kinase cassette that phosphorylates and inactivates the transcriptional coactivator Yorkie (Yki). H...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2011.12.011

    authors: Yue T,Tian A,Jiang J

    更新日期:2012-02-14 00:00:00

  • Human Centromeres Produce Chromosome-Specific and Array-Specific Alpha Satellite Transcripts that Are Complexed with CENP-A and CENP-C.

    abstract::Human centromeres are defined by alpha satellite DNA arrays that are distinct and chromosome specific. Most human chromosomes contain multiple alpha satellite arrays that are competent for centromere assembly. Here, we show that human centromeres are defined by chromosome-specific RNAs linked to underlying organizatio...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2017.07.001

    authors: McNulty SM,Sullivan LL,Sullivan BA

    更新日期:2017-08-07 00:00:00

  • Short-term integration of Cdc25 dynamics controls mitotic entry during Drosophila gastrulation.

    abstract::Cells commit to mitosis by abruptly activating the mitotic cyclin-Cdk complexes. During Drosophila gastrulation, mitosis is associated with the transcriptional activation of cdc25(string), a phosphatase that activates Cdk1. Here, we demonstrate that the switch-like entry into mitosis observed in the Drosophila embryo ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2012.01.019

    authors: Di Talia S,Wieschaus EF

    更新日期:2012-04-17 00:00:00

  • Functional genomic analysis of the periodic transcriptome in the developing Drosophila wing.

    abstract::The eukaryotic cell cycle, driven by both transcriptional and posttranslational mechanisms, is the central molecular oscillator underlying tissue growth throughout animals. Although genome-wide studies have investigated cell-cycle-associated transcription in unicellular systems, global patterns of periodic transcripti...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2014.02.018

    authors: Liang L,Haug JS,Seidel CW,Gibson MC

    更新日期:2014-04-14 00:00:00

  • A Self-Organized PLT/Auxin/ARR-B Network Controls the Dynamics of Root Zonation Development in Arabidopsis thaliana.

    abstract::During organogenesis, coherent organ growth arises from spatiotemporally coordinated decisions of individual cells. In the root of Arabidopsis thaliana, this coordination results in the establishment of a division and a differentiation zone. Cells continuously move through these zones; thus, a major question is how th...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2020.04.004

    authors: Salvi E,Rutten JP,Di Mambro R,Polverari L,Licursi V,Negri R,Dello Ioio R,Sabatini S,Ten Tusscher K

    更新日期:2020-05-18 00:00:00