Abstract：
:The interaction between the immune system and endocrine cells in the pancreas is crucial for the initiation and progression of type 1 diabetes (T1D). Imaging mass cytometry (IMC) enables multiplexed assessment of the abundance and localization of more than 30 proteins on the same tissue section at 1-μm resolution. Herein, we have developed a panel of 33 antibodies that allows for the quantification of key cell types including pancreatic exocrine cells, islet cells, immune cells, and stromal components. We employed this panel to analyze 12 pancreata obtained from donors with clinically diagnosed T1D and 6 pancreata from non-diabetic controls. In the pancreata from donors with T1D, we simultaneously visualized significant alterations in islet architecture, endocrine cell composition, and immune cell presentation. Indeed, we demonstrate the utility of IMC to investigate complex events on the cellular level that will provide new insights on the pathophysiology of T1D.
journal_name
Cell Metabjournal_title
Cell metabolismauthors
Wang YJ,Traum D,Schug J,Gao L,Liu C,HPAP Consortium.,Atkinson MA,Powers AC,Feldman MD,Naji A,Chang KM,Kaestner KHdoi
10.1016/j.cmet.2019.01.003subject
Has Abstractpub_date
2019-03-05 00:00:00pages
769-783.e4issue
3eissn
1550-4131issn
1932-7420pii
S1550-4131(19)30003-8journal_volume
29pub_type
杂志文章相关文献
Cell Metabolism文献大全abstract：:Prohibitins form large protein and lipid scaffolds in the inner membrane of mitochondria that are required for mitochondrial morphogenesis, neuronal survival, and normal lifespan. Here, we have defined the interactome of PHB2 in mitochondria and identified DNAJC19, mutated in dilated cardiomyopathy with ataxia, as bin...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2014.04.016
更新日期：2014-07-01 00:00:00
abstract：:Reverse cholesterol transport (RCT) is an antiatherogenic process in which excessive cholesterol from peripheral tissues is transported to the liver and finally excreted from the body via the bile. The nuclear receptor liver receptor homolog 1 (LRH-1) drives expression of genes regulating RCT, and its activity can be ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2014.07.023
更新日期：2014-10-07 00:00:00
abstract：:Lipoprotein cholesterol is mobilized from lysosomes by actions of the NPC1 and NPC2 proteins. In this issue of Cell Metabolism, Harrison et al. report on identification of an NPC2-interacting protein, the Nogo-B receptor, that regulates NPC2 protein levels. NPC2 stabilization may represent a novel mechanism through wh...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2009.08.006
更新日期：2009-09-01 00:00:00
abstract：:The biogenesis and function of the mitochondrial respiratory chain (RC) involve the organization of RC enzyme complexes in supercomplexes or respirasomes through an unknown biosynthetic process. This leads to structural interdependences between RC complexes, which are highly relevant from biological and biomedical per...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2012.01.015
更新日期：2012-03-07 00:00:00
abstract：:Nicotinamide phosphoribosyltransferase (NAMPT), the key NAD(+) biosynthetic enzyme, has two different forms, intra- and extracellular (iNAMPT and eNAMPT), in mammals. However, the significance of eNAMPT secretion remains unclear. Here we demonstrate that deacetylation of iNAMPT by the mammalian NAD(+)-dependent deacet...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2015.04.002
更新日期：2015-05-05 00:00:00
abstract：:Transgenerational inheritance of epigenetic characteristics in plants has been reported, whereas nongenetic persistence of complex phenotypes in animals is controversial. A recent report by Anne Brunet and colleagues describes a fascinating example of persistence across generations of extended life span in worm and ex...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2011.12.012
更新日期：2012-01-04 00:00:00
abstract：:It is still unclear if the ability of key regulators of actin cytoskeletal remodeling to influence lipid metabolism contributes to kidney injury. In this issue of Cell Metabolism, Fu et al. (2020) show that junctional adhesion molecule-like (JAML) is a novel mediator of glomerular disease progression while suggesting ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2020.11.002
更新日期：2020-12-01 00:00:00
abstract：:Although the concept of Syndrome X was introduced in the Banting Medal address of 1988 (Reaven, 1988), the notion that led to its genesis had started approximately 50 years earlier. In this short history, an attempt will be made to trace the two paths of scientific discovery that were formally merged in New Orleans in...
journal_title：Cell metabolism
pub_type： 历史文章,杂志文章
doi：10.1016/j.cmet.2004.12.001
更新日期：2005-01-01 00:00:00
abstract：:C. difficile infection is a deadly disease that is influenced by the microbiome. In a recent article in Nature, Buffie et al. (2014) demonstrate that the ability of C. scindens to synthesize secondary bile acids is crucial to providing resistance to C. difficile infection. ...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2014.12.016
更新日期：2015-01-06 00:00:00
abstract：:The contribution of interleukin (IL)-6 signaling in obesity-induced inflammation remains controversial. To specifically define the role of hepatic IL-6 signaling in insulin action and resistance, we have generated mice with hepatocyte-specific IL-6 receptor (IL-6R) alpha deficiency (IL-6Ralpha(L-KO) mice). These anima...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2010.06.011
更新日期：2010-09-08 00:00:00
abstract：:The role of microbial load during aging of the adult fruit fly Drosophila melanogaster is incompletely understood. Here we show dramatic increases in aerobic and anaerobic bacterial load during aging, both inside the body and on the surface. Scanning electron microscopy and cell staining analyses of the surface of age...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2007.06.006
更新日期：2007-08-01 00:00:00
abstract：:Metabolic imaging using hyperpolarized magnetic resonance can increase the sensitivity of MRI, though its ability to inform on relevant changes to biochemistry in humans remains unclear. In this work, we image pyruvate metabolism in patients, assessing the reproducibility of delivery and conversion in the setting of p...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2019.08.024
更新日期：2020-01-07 00:00:00
abstract：:Regulatory T cells (Tregs) subdue immune responses. Central to Treg activation are changes in lipid metabolism that support their survival and function. Fatty acid binding proteins (FABPs) are a family of lipid chaperones required to facilitate uptake and intracellular lipid trafficking. One family member, FABP5, is e...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2019.11.021
更新日期：2020-02-04 00:00:00
abstract：:Peroxisome proliferator-activated receptor gamma coactivator-1 beta (PGC-1beta) is known to be a transcriptional coactivator for SREBP-1, the master regulator of hepatic lipogenesis. Here, we evaluated the role of PGC-1beta in the pathogenesis of fructose-induced insulin resistance by using an antisense oligonucletoid...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2009.01.011
更新日期：2009-03-01 00:00:00
abstract：:Leptin secreted by adipocytes acts on the brain to reduce food intake by regulating neuronal activity in the mediobasal hypothalamus (MBH). Obesity is associated with resistance to high circulating leptin levels. Here, we demonstrate that peripherally administered leptin activates its receptor (LepR) in median eminenc...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2013.12.015
更新日期：2014-02-04 00:00:00
abstract：:Individuals with elevated LDL-cholesterol levels have an increased risk for cardiovascular disease. Despite lipid lowering strategies, however, a significant cardiovascular risk remains. Bekkering et al. show that monocytes from patients with familial hypercholesterolemia have a trained immunity phenotype and that lip...
journal_title：Cell metabolism
pub_type： 信件
doi：10.1016/j.cmet.2019.05.014
更新日期：2019-07-02 00:00:00
abstract：:Skin aging is of considerable interest from various perspectives, ranging from aesthetics to cancer development. Zhang et al. (2019) elucidate an immunological consequence of aging in the adipose layer of skin. Age-dependent increases in TGF-β signaling impair fibroblast adipogenic potential that results in impaired a...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2019.02.006
更新日期：2019-03-05 00:00:00
abstract：:Signaling cascades during adipogenesis culminate in the expression of two essential adipogenic factors, PPARgamma and C/EBPalpha. Here we demonstrate that the IRE1alpha-XBP1 pathway, the most conserved branch of the unfolded protein response (UPR), is indispensable for adipogenesis. Indeed, XBP1-deficient mouse embryo...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2009.04.009
更新日期：2009-06-01 00:00:00
abstract：:Light is a powerful synchronizer of the circadian rhythms, and bright light therapy is known to improve metabolic and hormonal status of circadian rhythm sleep disorders, although its mechanism is poorly understood. In the present study, we revealed that light induces gene expression in the adrenal gland via the supra...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2005.09.009
更新日期：2005-11-01 00:00:00
abstract：:Understanding the neural framework behind appetite control is fundamental to developing effective therapies to combat the obesity epidemic. The paraventricular hypothalamus (PVH) is critical for appetite regulation, yet, the real-time, physiological response properties of PVH neurons to nutrients are unknown. Using a ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2018.10.016
更新日期：2019-03-05 00:00:00
abstract：:Oxidative stress causes mitochondrial dysfunction and metabolic complications through unknown mechanisms. Cardiolipin (CL) is a key mitochondrial phospholipid required for oxidative phosphorylation. Oxidative damage to CL from pathological remodeling is implicated in the etiology of mitochondrial dysfunction commonly ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2010.07.003
更新日期：2010-08-04 00:00:00
abstract：:We investigated relationships among immune, metabolic, and sleep abnormalities in mice with non-metastatic mammary cancer. Tumor-bearing mice displayed interleukin-6 (IL-6)-mediated peripheral inflammation, coincident with altered hepatic glucose processing and sleep. Tumor-bearing mice were hyperphagic, had reduced s...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2018.04.021
更新日期：2018-07-03 00:00:00
abstract：:Intestinal microbial communities regulate a range of host physiological functions, from energy harvest and glucose homeostasis to immune development and regulation. Suez et al. (2014) recently demonstrated that artificial sweeteners alter gut microbial communities, leading to glucose intolerance in both mice and human...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2014.10.012
更新日期：2014-11-04 00:00:00
abstract：:Type 2 diabetes is a genetically heterogeneous disease, with several relatively rare monogenic forms and a number of more common forms resulting from a complex interaction of genetic and environmental factors. Previous studies using a candidate gene approach, family linkage studies, and gene expression profiling uncov...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2008.08.006
更新日期：2008-09-01 00:00:00
abstract：:Using molecular, biochemical, and untargeted stable isotope tracing approaches, we identify a previously unappreciated glutamine-derived α-ketoglutarate (αKG) energy-generating anaplerotic flux to be critical in mitochondrial DNA (mtDNA) mutant cells that harbor human disease-associated oxidative phosphorylation defec...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2018.03.002
更新日期：2018-05-01 00:00:00
abstract：:Components involved in vesicle trafficking processes such as secretion, endocytosis, and autophagy are gaining recognition as important regulators and effectors of target of rapamycin (TOR) signaling. A recent report by now implicates Pmr1, a secretory pathway Ca(2+)/Mn(2+) ATPase located in the Golgi apparatus, as a ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2006.12.008
更新日期：2007-01-01 00:00:00
abstract：:Metabolic reprogramming plays an important role in supporting tumor growth. However, little is known about the metabolic alterations that promote cancer metastasis. In this study, we identify acyl-CoA thioesterase 12 (ACOT12) as a key player in hepatocellular carcinoma (HCC) metastasis. The expression of ACOT12 is sig...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2018.12.019
更新日期：2019-04-02 00:00:00
abstract：:The mechanism by which pharmacologic administration of the hormone FGF21 increases energy expenditure to cause weight loss in obese animals is unknown. Here we report that FGF21 acts centrally to exert its effects on energy expenditure and body weight in obese mice. Using tissue-specific knockout mice, we show that βK...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2014.07.012
更新日期：2014-10-07 00:00:00
abstract：:How circulating signals of hunger and satiety enter the brain to reach neurons that govern energy balance has long remained a matter of controversy and speculation. Balland et al. (2014) now elucidate molecular mechanisms by which a highly specialized hypothalamic glial cell regulates transport of leptin across the bl...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2014.01.008
更新日期：2014-02-04 00:00:00
abstract：:Mutations resulting in reduced signaling of the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis are associated with increased life- and healthspan across model organisms. Similar findings have been noted in human cohorts with functional mutations in the somatotropic axis, suggesting that this pathway may a...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2016.05.014
更新日期：2016-06-14 00:00:00
