Tanshinone IIA reduces SW837 colorectal cancer cell viability via the promotion of mitochondrial fission by activating JNK-Mff signaling pathways.


BACKGROUND:Mitochondrial homeostasis has been increasingly viewed as a potential target of cancer therapy, and mitochondrial fission is a novel regulator of mitochondrial function and apoptosis. The aim of our study was to determine the detailed role of mitochondrial fission in SW837 colorectal cancer cell viability, mobility and proliferation. In addition, the current study also investigated the therapeutic impact of Tanshinone IIA (Tan IIA), a type of anticancer adjuvant drug, on cancer mitochondrial homeostasis. RESULTS:The results of our data illustrated that Tan IIA promoted SW837 cell death, impaired cell migration and mediated cancer proliferation arrest in a dose-dependent manner. Functional investigation exhibited that Tan IIA treatment evoked mitochondrial injury, as witnessed by mitochondrial ROS overproduction, mitochondrial potential collapse, antioxidant factor downregulation, mitochondrial pro-apoptotic protein upregulation, and caspase-9-dependent apoptotic pathway activation. Furthermore, we confirmed that Tan IIA mediated mitochondrial damage by activating mitochondrial fission, and the inhibition of mitochondrial fission abrogated the proapoptotic effects of Tan IIA on SW837 cells. To this end, our results demonstrated that Tan IIA modulated mitochondrial fission via the JNK-Mff pathways. The blockade of the JNK-Mff axis inhibited Tan IIA-mediated mitochondrial fission and promoted the survival of SW837 cells. CONCLUSIONS:Altogether, our results identified mitochondrial fission as a new potential target to control cancer viability, mobility and proliferation. Furthermore, our findings demonstrate that Tan IIA is an effective drug to treat colorectal cancer by activating JNK-Mff-mitochondrial fission pathways.


BMC Cell Biol


BMC cell biology


Jieensinue S,Zhu H,Li G,Dong K,Liang M,Li Y




Has Abstract


2018-09-25 00:00:00












  • Ca2+ regulation in the absence of the iplA gene product in Dictyostelium discoideum.

    abstract:BACKGROUND:Stimulation of Dictyostelium discoideum with cAMP evokes an elevation of the cytosolic free Ca2+ concentration ([Ca2+]i). The [Ca2+]i-change is composed of liberation of stored Ca2+ and extracellular Ca2+-entry. The significance of the [Ca2+]i-transient for chemotaxis is under debate. Abolition of chemotacti...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Schaloske RH,Lusche DF,Bezares-Roder K,Happle K,Malchow D,Schlatterer C

    更新日期:2005-03-11 00:00:00

  • Quantitative digital in situ senescence-associated β-galactosidase assay.

    abstract:BACKGROUND:Cellular senescence plays important roles in the aging process of complex organisms, in tumor suppression and in response to stress. Several markers can be used to identify senescent cells, of which the most widely used is the senescence-associated β-galactosidase (SABG) activity. The main advantage of SABG ...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Shlush LI,Itzkovitz S,Cohen A,Rutenberg A,Berkovitz R,Yehezkel S,Shahar H,Selig S,Skorecki K

    更新日期:2011-04-15 00:00:00

  • Two glutamic acid residues in the DNA-binding domain are engaged in the release of STAT1 dimers from DNA.

    abstract:BACKGROUND:In interferon-γ-stimulated cells, the dimeric transcription factor STAT1 (signal transducer and activator of transcription 1) recognizes semi-palindromic motifs in the promoter regions of cytokine-driven target genes termed GAS (gamma-activated sites). However, the molecular steps that facilitate GAS binding...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Koch V,Staab J,Ruppert V,Meyer T

    更新日期:2012-08-24 00:00:00

  • Nucleolar localization of an isoform of the IGF-I precursor.

    abstract::BACKGROUND Alternative exons encode different isoforms of the human insulin-like growth factor-I (IGF-I) precursor without altering mature IGF-I. We hypothesized that the various IGF-I precursors may traffic IGF-I differently. Chimeric IGF-I precursors were made with green fluorescent protein (GFP) cloned between the ...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Tan DS,Cook A,Chew SL

    更新日期:2002-07-02 00:00:00

  • Upregulation of Toll-like receptor (TLR) expression and release of cytokines from P815 mast cells by GM-CSF.

    abstract:BACKGROUND:Recently, mast cells have been recognized to express several Toll-like receptors (TLRs) on their membrane surfaces, and granulocyte-macrophage colony-stimulating factor (GM-CSF) was reported to be able to alter expression of TLRs and cytokine production in neutrophils. However, whether GM-CSF modulates the e...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Yang H,Wei J,Zhang H,Lin L,Zhang W,He S

    更新日期:2009-05-09 00:00:00

  • Non-enzymatic glycosylation of a type I collagen matrix: effects on osteoblastic development and oxidative stress.

    abstract:BACKGROUND:The tissue accumulation of protein-bound advanced glycation endproducts (AGE) may be involved in the etiology of diabetic chronic complications, including osteopenia. The aim of this study was to investigate the effect of an AGE-modified type I collagen substratum on the adhesion, spreading, proliferation an...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: McCarthy AD,Etcheverry SB,Bruzzone L,Lettieri G,Barrio DA,Cortizo AM

    更新日期:2001-01-01 00:00:00

  • Interlobular and intralobular mammary stroma: genotype may not reflect phenotype.

    abstract:BACKGROUND:The normal growth and function of mammary epithelial cells depend on interactions with the supportive stroma. Alterations in this communication can lead to the progression or expansion of malignant growth. The human mammary gland contains two distinctive types of fibroblasts within the stroma. The epithelial...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Fleming JM,Long EL,Ginsburg E,Gerscovich D,Meltzer PS,Vonderhaar BK

    更新日期:2008-08-18 00:00:00

  • Monkey hybrid stem cells develop cellular features of Huntington's disease.

    abstract:BACKGROUND:Pluripotent stem cells that are capable of differentiating into different cell types and develop robust hallmark cellular features are useful tools for clarifying the impact of developmental events on neurodegenerative diseases such as Huntington's disease. Additionally, a Huntington's cell model that develo...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Laowtammathron C,Cheng ECh,Cheng PH,Snyder BR,Yang SH,Johnson Z,Lorthongpanich C,Kuo HC,Parnpai R,Chan AW

    更新日期:2010-02-05 00:00:00

  • Effect of cAMP derivates on assembly and maintenance of tight junctions in human umbilical vein endothelial cells.

    abstract:BACKGROUND:Endothelial tight and adherens junctions control a variety of physiological processes like adhesion, paracellular transport of solutes or trafficking of activated leukocytes. Formation and maintenance of endothelial junctions largely depend on the microenvironment of the specific vascular bed and on interact...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Beese M,Wyss K,Haubitz M,Kirsch T

    更新日期:2010-09-07 00:00:00

  • Control of CXCR2 activity through its ubiquitination on K327 residue.

    abstract:BACKGROUND:The interleukin-8 chemokine (IL-8) G-protein coupled receptor CXCR2 governs pro-inflammatory and pro-angiogenic responses in leukocytes and endothelial cells. At a molecular standpoint, CXCR2 is widely reported to operate through calcium flux, phosphoinoisitide 3 kinase (PI3K) and mitogen-activated protein k...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Leclair HM,Dubois SM,Azzi S,Dwyer J,Bidère N,Gavard J

    更新日期:2014-10-22 00:00:00

  • Ligands specify estrogen receptor alpha nuclear localization and degradation.

    abstract:BACKGROUND:The estrogen receptor alpha (ERα) is found predominately in the nucleus, both in hormone stimulated and untreated cells. Intracellular distribution of the ERα changes in the presence of agonists but the impact of different antiestrogens on the fate of ERα is a matter of debate. RESULTS:A MCF-7 cell line sta...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Kocanova S,Mazaheri M,Caze-Subra S,Bystricky K

    更新日期:2010-12-10 00:00:00

  • Melatonin enhances DNA repair capacity possibly by affecting genes involved in DNA damage responsive pathways.

    abstract:BACKGROUND:Melatonin, a hormone-like substance involved in the regulation of the circadian rhythm, has been demonstrated to protect cells against oxidative DNA damage and to inhibit tumorigenesis. RESULTS:In the current study, we investigated the effect of melatonin on DNA strand breaks using the alkaline DNA comet as...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Liu R,Fu A,Hoffman AE,Zheng T,Zhu Y

    更新日期:2013-01-07 00:00:00

  • Hypoxia-mimetic agents inhibit proliferation and alter the morphology of human umbilical cord-derived mesenchymal stem cells.

    abstract:BACKGROUND:The therapeutic efficacy of human mesenchymal stem cells (hMSCs) for the treatment of hypoxic-ischemic diseases is closely related to level of hypoxia in the damaged tissues. To elucidate the potential therapeutic applications and limitations of hMSCs derived from human umbilical cords, the effects of hypoxi...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Zeng HL,Zhong Q,Qin YL,Bu QQ,Han XA,Jia HT,Liu HW

    更新日期:2011-08-09 00:00:00

  • Crosstalk between the actin cytoskeleton and Ran-mediated nuclear transport.

    abstract:BACKGROUND:Transport of macromolecules into and out of the nucleus is a highly regulated process. The RanGTP/RanGDP gradient controls the trafficking of molecules exceeding the diffusion limit of the nuclear pore across the nuclear envelope. RESULTS:We found genetic interaction between genes establishing the Ran gradi...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Minakhina S,Myers R,Druzhinina M,Steward R

    更新日期:2005-08-24 00:00:00

  • Tissue and subcellular distribution of CLIC1.

    abstract:BACKGROUND:CLIC1 is a chloride channel whose cellular role remains uncertain. The distribution of CLIC1 in normal tissues is largely unknown and conflicting data have been reported regarding the cellular membrane fraction in which CLIC1 resides. RESULTS:New antisera to CLIC1 were generated and were found to be sensiti...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Ulmasov B,Bruno J,Woost PG,Edwards JC

    更新日期:2007-02-27 00:00:00

  • The interaction of Kinesin-1 with its adaptor protein JIP1 can be regulated via proteins binding to the JIP1-PTB domain.

    abstract:BACKGROUND:The regulatory mechanisms of motor protein-dependent intracellular transport are still not fully understood. The kinesin-1-binding protein, JIP1, can function as an adaptor protein that links kinesin-1 and other JIP1-binding "cargo" proteins. However, it is unknown whether these "cargo" proteins influence th...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Satake T,Otsuki K,Banba Y,Suenaga J,Hirano H,Yamanaka Y,Ohno S,Hirai S

    更新日期:2013-03-04 00:00:00

  • Differentiation potential of STRO-1+ dental pulp stem cells changes during cell passaging.

    abstract:BACKGROUND:Dental pulp stem cells (DPSCs) can be driven into odontoblast, osteoblast, and chondrocyte lineages in different inductive media. However, the differentiation potential of naive DPSCs after serial passaging in the routine culture system has not been fully elucidated. RESULTS:DPSCs were isolated from human/r...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Yu J,He H,Tang C,Zhang G,Li Y,Wang R,Shi J,Jin Y

    更新日期:2010-05-08 00:00:00

  • Involvement of CD147 in overexpression of MMP-2 and MMP-9 and enhancement of invasive potential of PMA-differentiated THP-1.

    abstract:BACKGROUND:During infection and inflammation, circulating blood monocytes migrate from the intravascular compartments to the extravascular compartments, where they mature into tissue macrophages. The maturation process prepares the cells to actively participate in the inflammatory and immune responses, and many factors...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Zhou J,Zhu P,Jiang JL,Zhang Q,Wu ZB,Yao XY,Tang H,Lu N,Yang Y,Chen ZN

    更新日期:2005-05-17 00:00:00

  • Fas induces apoptosis in human coronary artery endothelial cells in vitro.

    abstract:BACKGROUND:Published work suggests that some types of endothelial cells undergo apoptosis in response to ligation of the receptor Fas (CD95, APO1) but other types are resistant. Because heterogeneity among endothelial cells from different tissues, has been demonstrated, the purpose of this study was to determine, if Fa...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Filippatos G,Ang E,Gidea C,Dincer E,Wang R,Uhal BD

    更新日期:2004-01-22 00:00:00

  • Glycosylation, transport, and complex formation of palmitoyl protein thioesterase 1 (PPT1)--distinct characteristics in neurons.

    abstract:BACKGROUND:Neuronal ceroid lipofuscinoses (NCLs) are collectively the most common type of recessively inherited childhood encephalopathies. The most severe form of NCL, infantile neuronal ceroid lipofuscinosis (INCL), is caused by mutations in the CLN1 gene, resulting in a deficiency of the lysosomal enzyme, palmitoyl ...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Lyly A,von Schantz C,Salonen T,Kopra O,Saarela J,Jauhiainen M,Kyttälä A,Jalanko A

    更新日期:2007-06-12 00:00:00

  • Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells.

    abstract:BACKGROUND:There is a growing interest in cord blood as a source of primitive stem cells with the capacity for multilineage differentiation. Pure cell fractions are needed for the characterization and in vitro expansion of stem cells as well as for their use in preclinical research. However, enrichment of stem cells is...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Kekarainen T,Mannelin S,Laine J,Jaatinen T

    更新日期:2006-08-01 00:00:00

  • Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters.

    abstract:BACKGROUND:We have previously obtained a clonal population of cells from human foreskin that is able to differentiate into mesodermal, ectodermal and endodermal progenies. It is of great interest to know whether these cells could be further differentiated into functional insulin-producing cells. RESULTS:Sixty-one sing...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Bi D,Chen FG,Zhang WJ,Zhou GD,Cui L,Liu W,Cao Y

    更新日期:2010-06-25 00:00:00

  • Inclusion of Scar/WAVE3 in a similar complex to Scar/WAVE1 and 2.

    abstract:BACKGROUND:The Scar/WAVE family of proteins mediates signals to actin assembly by direct activation of the Arp2/3 complex. These proteins have been characterised as major regulators of lamellipodia formation downstream of Rac activation and as members of large protein complexes. RESULTS:We have investigated the intera...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Stovold CF,Millard TH,Machesky LM

    更新日期:2005-03-07 00:00:00

  • Xanthurenic acid translocates proapoptotic Bcl-2 family proteins into mitochondria and impairs mitochondrial function.

    abstract:BACKGROUND:Xanthurenic acid is an endogenous molecule produced by tryptophan degradation, produced in the cytoplasm and mitochondria. Its accumulation can be observed in aging-related diseases, e.g. senile cataract and infectious disease. We previously reported that xanthurenic acid provokes apoptosis, and now present ...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Malina HZ,Hess OM

    更新日期:2004-04-06 00:00:00

  • Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression.

    abstract:BACKGROUND:Nuclear size and shape are specific to a cell type, function, and location, and can serve as indicators of disease and development. We previously found that lamin A/C and associated nuclear envelope structural proteins were upregulated when murine embryonic stem (ES) cells differentiated to primitive endoder...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Smith ER,Meng Y,Moore R,Tse JD,Xu AG,Xu XX

    更新日期:2017-01-14 00:00:00

  • Clathrin expression in Trypanosoma cruzi.

    abstract:BACKGROUND:Clathrin-mediated vesicular trafficking, the mechanism by which proteins and lipids are transported between membrane-bound organelles, accounts for a large proportion of import from the plasma membrane (endocytosis) and transport from the trans-Golgi network towards the endosomal system. Clathrin-mediated ev...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Kalb LC,Frederico YC,Batista CM,Eger I,Fragoso SP,Soares MJ

    更新日期:2014-06-19 00:00:00

  • Smad7 and protein phosphatase 1alpha are critical determinants in the duration of TGF-beta/ALK1 signaling in endothelial cells.

    abstract:BACKGROUND:In endothelial cells (EC), transforming growth factor-beta (TGF-beta) can bind to and transduce signals through ALK1 and ALK5. The TGF-beta/ALK5 and TGF-beta/ALK1 pathways have opposite effects on EC behaviour. Besides differential receptor binding, the duration of TGF-beta signaling is an important specific...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Valdimarsdottir G,Goumans MJ,Itoh F,Itoh S,Heldin CH,ten Dijke P

    更新日期:2006-03-29 00:00:00

  • A STAT3-decoy oligonucleotide induces cell death in a human colorectal carcinoma cell line by blocking nuclear transfer of STAT3 and STAT3-bound NF-κB.

    abstract:BACKGROUND:The transcription factor STAT3 (signal transducer and activator of transcription 3) is frequently activated in tumor cells. Activated STAT3 forms homodimers, or heterodimers with other TFs such as NF-κB, which becomes activated. Cytoplasmic STAT3 dimers are activated by tyrosine phosphorylation; they interac...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Souissi I,Najjar I,Ah-Koon L,Schischmanoff PO,Lesage D,Le Coquil S,Roger C,Dusanter-Fourt I,Varin-Blank N,Cao A,Metelev V,Baran-Marszak F,Fagard R

    更新日期:2011-04-12 00:00:00

  • In vitro three-dimensional modeling of fallopian tube secretory epithelial cells.

    abstract:BACKGROUND:Fallopian tube secretory epithelial cells (FTSECs) have been implicated as a cell-of-origin for high-grade serous epithelial ovarian cancer. However, there are relatively few in vitro models of this tissue type available for use in studies of FTSEC biology and malignant transformation. In vitro three-dimensi...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Lawrenson K,Notaridou M,Lee N,Benjamin E,Jacobs IJ,Jones C,Gayther SA

    更新日期:2013-09-27 00:00:00

  • Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.

    abstract:BACKGROUND:Multilineage differentiation, immunomodulation and secretion of trophic factors render mesenchymal stromal cells (MSC) highly attractive for clinical application. Human platelet derivatives such as pooled human platelet lysate (pHPL) and thrombin-activated platelet releasate in plasma (tPRP) have been introd...

    journal_title:BMC cell biology

    pub_type: 杂志文章


    authors: Kinzebach S,Dietz L,Klüter H,Thierse HJ,Bieback K

    更新日期:2013-10-30 00:00:00